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The map label for this gene is betT [H]

Identifier: 119774312

GI number: 119774312

Start: 1435364

End: 1437340

Strand: Direct

Name: betT [H]

Synonym: Sama_1175

Alternate gene names: 119774312

Gene position: 1435364-1437340 (Clockwise)

Preceding gene: 119774310

Following gene: 119774313

Centisome position: 33.33

GC content: 53.77

Gene sequence:

>1977_bases
ATGGCACTCAAATACAGTATTAATCGGCCGGTTTTTTTCGGTTCTCTTTTTCTTATCACCCTCCTGGTGGCGCTCGGTGC
CATCTGGCCGCAGCAGGCTCAACAATGGTTTGGTGCAGTGCAGCATTGGCTTGAAGTCAAAGCTGGCTGGCTTTATGTGC
TCGGTGTGGCCGTCTTTCTGGTTTTTATTGTCTTTGTGATGGTCAGCCGTTTCGGTGATATCAAGCTTGGTCCCGATCAT
GCCGAGCCCGACTACAGCTATAAGAGCTGGATAGCCATGCTGTTTTCGGCTGGCATGGGGATAGGGCTCATGTTTTTCGG
GGTGGCAGAGCCTGTTATGCACCTGATAGCCCCGCCGGATGCTACGCCCGAGTCAATAGAGGCCGCCAGGCAGGCGATGA
AAATTACCTTCTTTCACTGGGGCATTCATGCCTGGGCCATCTATGCTGTGGTGGCATTGAGTCTGGCTTACTTCGCCTAT
CGGCATAAGTTACCGCTGCTGCCAAGAAGCGCGCTTTATCCCTTGATTGGCGAGCGTATTCACGGACCGATTGGCCATGC
GGTGGATGCGTTTGCCGTGCTGGGCACCATGTTTGGTGTTGCCACATCGCTGGGTTTTGGTGTGTTGCAGGTGAATGCCG
GGCTTAATTTTCTTTTCGGTGACCAGTTTCCGGTCAGTACCCCCCTGCAGGTGGGGCTCATTGCGGTCATTACCCTGATT
GCCACAGTGTCTGTGTTCTCCGGGCTTGATAAAGGGGTTAAGCGCCTCAGCGAGCTCAATCTGCTGTTGGCGGTGGTATT
GATGCTGGTCGTGCTGCTGGTGGGGCCTACTGTGGCGCTGCTGCAGGCCATTGTACAAAACACTGGTGCTTATTTCTCTG
ATATTGTTGGCAAAACATTTAATCTATACGCCTATCAGCAGAAAAACGACTGGCTGGGCGGTTGGACCTTACTTTACTGG
GGGTGGTGGATTTCCTGGTCGCCTTTTGTGGGCACCTTTATTGCGCGGGTCTCCCGTGGCCGCACTATTCGTGAGTTTCT
GCTGGGGGTGCTCTTTGTGCCCAGTGGCTTTACCTTTTTGTGGATGACAGTATTTGGCAACTCGGCCATAGACCAGATTT
TGCATCAGGGTAATACAGTGCTCGCCGATGCCGTATCCAGCGACGTGTCTGTGGCGCTGTTTGTGTTCTTCCAGCAGTTG
CCGCTCACATCCTTGCTCTCGGCGGTGGCCCTGTGTCTGGTGGTCACCTTCTTTGTGACATCTTCCGATTCCGGCAGTCT
GGTGATTGATAACCTCACTTCCGGTGGCGATATGTCGTCCCCTGTATGGCAGCGCGTGTTTTGGGCGCTGATGCAGGGCG
TAGTGGCTTCTGTGCTCTTGCTGGCTGGCGGCTTGCAGGCACTGCAAACCGCTGCCATTGCCAGCGCCATGCCCTTTTTG
TTAGTGATGTTGCTGATTTGCCTTGGGCTTTTTAAGGCACTGCAGGATGACTGGCTTAAGCTATCCAGTGTGCAGTTGCA
TCACACCAGTGTGCAGTATGCCAGTGCCAACGTTGAATGGCGGGAGCGTCTGAAAGTGTTGGTGAATCAACCTTCCGCCA
AAGATGCCCGGGCCTTTATCCGCGGGCCTGCCCGCCAGGCGTTGAAGCAAGTGGCTTCTGCCTTCGTGAATGAAGGTATT
GATGCCCGCGTGGTGCAGCAAGAGGACAGGGTCAGGCTGCTGATTGCCAGCGACAATCATCCCGACTTCGTTTATGGTCT
GCGGCTGCGGCAGTATTCTAAAGTGCCGGTCCATGGTGTGGAGGAGACCGACGATGACTTTTACCGGGTGGAAGTGTTTT
TGGAGCACGGTGGACAGCATTATGATGTGCTTGGCTACACAGTTGAGCAGTTGCAGGCCGATGCAGTAACTCAGTATGAA
AAATATCTGCACTATCTGCATTTATCCGTGAGTGAAGCGGTTAACCCGGATAGCTGA

Upstream 100 bases:

>100_bases
GTCGCCTCTCCAGCAATTTGTGCAGGACCAGCCCCGACATTTCAGCTGTTTATATCAGTGTCACCAGGGACGAGGTTCTG
CCTTAGCCAACGGAGCTATA

Downstream 100 bases:

>100_bases
TGCGCCACGATTGCACCGGACTGATTGGCGCGTTATGCTGCGCCCAACATAGGCTGTAACCTGACTTTTATGACCCAAAG
TAAACTCGATAAGGTATTGG

Product: choline/carnitine/betaine transport

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 658; Mature: 657

Protein sequence:

>658_residues
MALKYSINRPVFFGSLFLITLLVALGAIWPQQAQQWFGAVQHWLEVKAGWLYVLGVAVFLVFIVFVMVSRFGDIKLGPDH
AEPDYSYKSWIAMLFSAGMGIGLMFFGVAEPVMHLIAPPDATPESIEAARQAMKITFFHWGIHAWAIYAVVALSLAYFAY
RHKLPLLPRSALYPLIGERIHGPIGHAVDAFAVLGTMFGVATSLGFGVLQVNAGLNFLFGDQFPVSTPLQVGLIAVITLI
ATVSVFSGLDKGVKRLSELNLLLAVVLMLVVLLVGPTVALLQAIVQNTGAYFSDIVGKTFNLYAYQQKNDWLGGWTLLYW
GWWISWSPFVGTFIARVSRGRTIREFLLGVLFVPSGFTFLWMTVFGNSAIDQILHQGNTVLADAVSSDVSVALFVFFQQL
PLTSLLSAVALCLVVTFFVTSSDSGSLVIDNLTSGGDMSSPVWQRVFWALMQGVVASVLLLAGGLQALQTAAIASAMPFL
LVMLLICLGLFKALQDDWLKLSSVQLHHTSVQYASANVEWRERLKVLVNQPSAKDARAFIRGPARQALKQVASAFVNEGI
DARVVQQEDRVRLLIASDNHPDFVYGLRLRQYSKVPVHGVEETDDDFYRVEVFLEHGGQHYDVLGYTVEQLQADAVTQYE
KYLHYLHLSVSEAVNPDS

Sequences:

>Translated_658_residues
MALKYSINRPVFFGSLFLITLLVALGAIWPQQAQQWFGAVQHWLEVKAGWLYVLGVAVFLVFIVFVMVSRFGDIKLGPDH
AEPDYSYKSWIAMLFSAGMGIGLMFFGVAEPVMHLIAPPDATPESIEAARQAMKITFFHWGIHAWAIYAVVALSLAYFAY
RHKLPLLPRSALYPLIGERIHGPIGHAVDAFAVLGTMFGVATSLGFGVLQVNAGLNFLFGDQFPVSTPLQVGLIAVITLI
ATVSVFSGLDKGVKRLSELNLLLAVVLMLVVLLVGPTVALLQAIVQNTGAYFSDIVGKTFNLYAYQQKNDWLGGWTLLYW
GWWISWSPFVGTFIARVSRGRTIREFLLGVLFVPSGFTFLWMTVFGNSAIDQILHQGNTVLADAVSSDVSVALFVFFQQL
PLTSLLSAVALCLVVTFFVTSSDSGSLVIDNLTSGGDMSSPVWQRVFWALMQGVVASVLLLAGGLQALQTAAIASAMPFL
LVMLLICLGLFKALQDDWLKLSSVQLHHTSVQYASANVEWRERLKVLVNQPSAKDARAFIRGPARQALKQVASAFVNEGI
DARVVQQEDRVRLLIASDNHPDFVYGLRLRQYSKVPVHGVEETDDDFYRVEVFLEHGGQHYDVLGYTVEQLQADAVTQYE
KYLHYLHLSVSEAVNPDS
>Mature_657_residues
ALKYSINRPVFFGSLFLITLLVALGAIWPQQAQQWFGAVQHWLEVKAGWLYVLGVAVFLVFIVFVMVSRFGDIKLGPDHA
EPDYSYKSWIAMLFSAGMGIGLMFFGVAEPVMHLIAPPDATPESIEAARQAMKITFFHWGIHAWAIYAVVALSLAYFAYR
HKLPLLPRSALYPLIGERIHGPIGHAVDAFAVLGTMFGVATSLGFGVLQVNAGLNFLFGDQFPVSTPLQVGLIAVITLIA
TVSVFSGLDKGVKRLSELNLLLAVVLMLVVLLVGPTVALLQAIVQNTGAYFSDIVGKTFNLYAYQQKNDWLGGWTLLYWG
WWISWSPFVGTFIARVSRGRTIREFLLGVLFVPSGFTFLWMTVFGNSAIDQILHQGNTVLADAVSSDVSVALFVFFQQLP
LTSLLSAVALCLVVTFFVTSSDSGSLVIDNLTSGGDMSSPVWQRVFWALMQGVVASVLLLAGGLQALQTAAIASAMPFLL
VMLLICLGLFKALQDDWLKLSSVQLHHTSVQYASANVEWRERLKVLVNQPSAKDARAFIRGPARQALKQVASAFVNEGID
ARVVQQEDRVRLLIASDNHPDFVYGLRLRQYSKVPVHGVEETDDDFYRVEVFLEHGGQHYDVLGYTVEQLQADAVTQYEK
YLHYLHLSVSEAVNPDS

Specific function: High-affinity uptake of choline driven by a proton- motive force [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=658, Percent_Identity=40.7294832826748, Blast_Score=503, Evalue=1e-143,
Organism=Escherichia coli, GI1786224, Length=508, Percent_Identity=27.1653543307087, Blast_Score=176, Evalue=4e-45,
Organism=Escherichia coli, GI1788102, Length=479, Percent_Identity=28.8100208768267, Blast_Score=173, Evalue=3e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 72576; Mature: 72445

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS01303 BCCT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALKYSINRPVFFGSLFLITLLVALGAIWPQQAQQWFGAVQHWLEVKAGWLYVLGVAVFL
CCEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VFIVFVMVSRFGDIKLGPDHAEPDYSYKSWIAMLFSAGMGIGLMFFGVAEPVMHLIAPPD
HHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC
ATPESIEAARQAMKITFFHWGIHAWAIYAVVALSLAYFAYRHKLPLLPRSALYPLIGERI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
HGPIGHAVDAFAVLGTMFGVATSLGFGVLQVNAGLNFLFGDQFPVSTPLQVGLIAVITLI
CCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEECCCCCCCCCHHHHHHHHHHHH
ATVSVFSGLDKGVKRLSELNLLLAVVLMLVVLLVGPTVALLQAIVQNTGAYFSDIVGKTF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHCCCE
NLYAYQQKNDWLGGWTLLYWGWWISWSPFVGTFIARVSRGRTIREFLLGVLFVPSGFTFL
EEEEEECCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHH
WMTVFGNSAIDQILHQGNTVLADAVSSDVSVALFVFFQQLPLTSLLSAVALCLVVTFFVT
HHHHHCCHHHHHHHHCCCCEEHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHC
SSDSGSLVIDNLTSGGDMSSPVWQRVFWALMQGVVASVLLLAGGLQALQTAAIASAMPFL
CCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
LVMLLICLGLFKALQDDWLKLSSVQLHHTSVQYASANVEWRERLKVLVNQPSAKDARAFI
HHHHHHHHHHHHHHHHHHHHHHCEEEECCCHHHHCCCCHHHHHHHHHHCCCCCHHHHHHH
RGPARQALKQVASAFVNEGIDARVVQQEDRVRLLIASDNHPDFVYGLRLRQYSKVPVHGV
CCHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEECCCCCCCEEHHHHHHCCCCCCCCC
EETDDDFYRVEVFLEHGGQHYDVLGYTVEQLQADAVTQYEKYLHYLHLSVSEAVNPDS
CCCCCCCEEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ALKYSINRPVFFGSLFLITLLVALGAIWPQQAQQWFGAVQHWLEVKAGWLYVLGVAVFL
CEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH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CCCCCCCEEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]