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The map label for this gene is proS

Identifier: 119774298

GI number: 119774298

Start: 1416155

End: 1417873

Strand: Reverse

Name: proS

Synonym: Sama_1161

Alternate gene names: 119774298

Gene position: 1417873-1416155 (Counterclockwise)

Preceding gene: 119774299

Following gene: 119774297

Centisome position: 32.93

GC content: 56.37

Gene sequence:

>1719_bases
ATGCGAGTCAGCAAATACCTGCTATCGACACAGAAAGAAACCCCAGCCAATGCCGAGGTAGTCAGTCATCAACTCATGCT
GCGTGCGGGCATGATCCGCCGCAATGCGTCAGGCCTTTACAGCTACCTGCCCACAGGTTTGCGGGTACTGCGCAAAGTAG
AAGCTATTGTTCGCGAAGAAATGAACAAGGCTGGCGCCATTGAGATCCTGATGCCCATGGTGCAGCCGGGTGATTTGTGG
GTCGAAACAGGTCGTTGGGATCAGTTTGGTCCCGAGCTTTTGCGCTTTACCGACCGCCACAACCGCGACTTCGTGCTGGG
CCCAACCCACGAAGAAGTGATCACTGACCTTATCCGTAAAGAAGTAAGCTCGTACAAGCAGCTGCCGCTGAACCTGTATC
AGATCCAGACCAAGTTCCGTGACGAAGTGCGCCCCCGTTTCGGTGTGATGCGCTCACGGGAATTCCTGATGAAGGATGCC
TACTCCTTCCACCTGACACAGGAGACTCTGGACGAAACCTATCAGGCCATGTATACCGCCTACAGTAACATCTTCAGCCG
TATGGGTCTGGCATTCCGCCCCGTGCTGGCCGATACCGGCTCCATCGGTGGCAGCGTGTCCCACGAATTCCACGTACTGG
CCCAGAGCGGTGAAGACCTGATTGCCTACTCCACCGGCAGCGATTACGCCGCCAACATCGAGAAGGCCGAAGCGCCCCTG
CCCACCGAGCCTCGCGGCGAAGCAACCGAAGCGCTGCGCACCGTGGACACCCCCAATGCCAAAACCATCGCCGAGTTGGT
TGAGCAGTTTGGCGTGGCCATCGAAAAGACCATCAAGACTTTGATTGTTAAAGGCGCCACCGAAGAAGCCCCTCTGGTTG
CTCTGCTGGTTCGTGGCGACCACGAACTGAACGAAATCAAGGCCGACAAGCTGGAACTGGTGGCCTCGCCGCTGGAATTT
GCCGGTGAAGCTGAAATCCGCGCCGCCGTGGGTGCTGGTACCGGCTCTATCGGTCCGGTTAACCTCAAGATGCCTATCAT
TGCTGACCACAGCGTACTGGTAATGAGCGACTTCGCCGCCGGTGCCAACGAAGATGGCAAGCACTTCTTCGGTATCAACT
GGGAGCGCGATCTGCCGCTGGTTCAGGGCGCCGACATCCGCAACGTCGTAGAAGGCGAAGCCACACCCGATGGCAAGGGC
ACCTATGCCTTTGCCCGCGGTATCGAAGTGGGTCACATCTTCCAACTGGGTAACAAGTACTCAGAAGCCATGAACGCCAC
CGTACTTGATGAAAACGGCAAGTCACAGATCATGCTGATGGGCTGTTATGGTGTGGGCGTGAGCCGTATCGTGGCCGCTG
CCATCGAGCAGAACAATGACGAGCGCGGCATTGTATGGCCTGAGGCCATTGCCCCGTTCACCGTTGGCATTTTGCCGATG
AACATGCACAAGTCTCATCGGGTTACCGATACCGCTGAAGCCCTGTACAAAGAACTGACCGAAGCCGGTTTCGAAGTACT
GTTTGACGACCGTAAAGAGCGCCCTGGCGTGATGTTTGCCGATATGGAGCTGCTGGGCATACCTCACACTGTGGTCATAG
GCGATCGCAACATAGACACAGGTGTCTATGAGTACAAGAATCGCCGCACCGGTGAAAAGACCGAAGTGCCTTTTGCAGAG
CTGGTGAGCTTCCTCAAAGCCCAGTTTCAGCAAGGCTAA

Upstream 100 bases:

>100_bases
AAACTCACGTCTGGTCAGGGTTTTTGCTGCATGCTGAGCGTGACAACACAGATGCAGGTGATACAATGCAGGCCATTCTT
TTCAACTATGGACACCGGGA

Downstream 100 bases:

>100_bases
GGCTTAAAAAGATTCATAAAGGCACCCTTCGGGGTGCTTTTTTTTGCCTGCAGCATTGCCAACACTGTCAGCGAACGGCT
TTAATTAGCCTAGGGAAAGA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 572; Mature: 572

Protein sequence:

>572_residues
MRVSKYLLSTQKETPANAEVVSHQLMLRAGMIRRNASGLYSYLPTGLRVLRKVEAIVREEMNKAGAIEILMPMVQPGDLW
VETGRWDQFGPELLRFTDRHNRDFVLGPTHEEVITDLIRKEVSSYKQLPLNLYQIQTKFRDEVRPRFGVMRSREFLMKDA
YSFHLTQETLDETYQAMYTAYSNIFSRMGLAFRPVLADTGSIGGSVSHEFHVLAQSGEDLIAYSTGSDYAANIEKAEAPL
PTEPRGEATEALRTVDTPNAKTIAELVEQFGVAIEKTIKTLIVKGATEEAPLVALLVRGDHELNEIKADKLELVASPLEF
AGEAEIRAAVGAGTGSIGPVNLKMPIIADHSVLVMSDFAAGANEDGKHFFGINWERDLPLVQGADIRNVVEGEATPDGKG
TYAFARGIEVGHIFQLGNKYSEAMNATVLDENGKSQIMLMGCYGVGVSRIVAAAIEQNNDERGIVWPEAIAPFTVGILPM
NMHKSHRVTDTAEALYKELTEAGFEVLFDDRKERPGVMFADMELLGIPHTVVIGDRNIDTGVYEYKNRRTGEKTEVPFAE
LVSFLKAQFQQG

Sequences:

>Translated_572_residues
MRVSKYLLSTQKETPANAEVVSHQLMLRAGMIRRNASGLYSYLPTGLRVLRKVEAIVREEMNKAGAIEILMPMVQPGDLW
VETGRWDQFGPELLRFTDRHNRDFVLGPTHEEVITDLIRKEVSSYKQLPLNLYQIQTKFRDEVRPRFGVMRSREFLMKDA
YSFHLTQETLDETYQAMYTAYSNIFSRMGLAFRPVLADTGSIGGSVSHEFHVLAQSGEDLIAYSTGSDYAANIEKAEAPL
PTEPRGEATEALRTVDTPNAKTIAELVEQFGVAIEKTIKTLIVKGATEEAPLVALLVRGDHELNEIKADKLELVASPLEF
AGEAEIRAAVGAGTGSIGPVNLKMPIIADHSVLVMSDFAAGANEDGKHFFGINWERDLPLVQGADIRNVVEGEATPDGKG
TYAFARGIEVGHIFQLGNKYSEAMNATVLDENGKSQIMLMGCYGVGVSRIVAAAIEQNNDERGIVWPEAIAPFTVGILPM
NMHKSHRVTDTAEALYKELTEAGFEVLFDDRKERPGVMFADMELLGIPHTVVIGDRNIDTGVYEYKNRRTGEKTEVPFAE
LVSFLKAQFQQG
>Mature_572_residues
MRVSKYLLSTQKETPANAEVVSHQLMLRAGMIRRNASGLYSYLPTGLRVLRKVEAIVREEMNKAGAIEILMPMVQPGDLW
VETGRWDQFGPELLRFTDRHNRDFVLGPTHEEVITDLIRKEVSSYKQLPLNLYQIQTKFRDEVRPRFGVMRSREFLMKDA
YSFHLTQETLDETYQAMYTAYSNIFSRMGLAFRPVLADTGSIGGSVSHEFHVLAQSGEDLIAYSTGSDYAANIEKAEAPL
PTEPRGEATEALRTVDTPNAKTIAELVEQFGVAIEKTIKTLIVKGATEEAPLVALLVRGDHELNEIKADKLELVASPLEF
AGEAEIRAAVGAGTGSIGPVNLKMPIIADHSVLVMSDFAAGANEDGKHFFGINWERDLPLVQGADIRNVVEGEATPDGKG
TYAFARGIEVGHIFQLGNKYSEAMNATVLDENGKSQIMLMGCYGVGVSRIVAAAIEQNNDERGIVWPEAIAPFTVGILPM
NMHKSHRVTDTAEALYKELTEAGFEVLFDDRKERPGVMFADMELLGIPHTVVIGDRNIDTGVYEYKNRRTGEKTEVPFAE
LVSFLKAQFQQG

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=214, Percent_Identity=46.2616822429907, Blast_Score=211, Evalue=2e-54,
Organism=Escherichia coli, GI1786392, Length=571, Percent_Identity=71.4535901926445, Blast_Score=846, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115532348, Length=259, Percent_Identity=38.6100386100386, Blast_Score=177, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI193203271, Length=105, Percent_Identity=36.1904761904762, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6320931, Length=202, Percent_Identity=40.5940594059406, Blast_Score=174, Evalue=3e-44,
Organism=Drosophila melanogaster, GI24656200, Length=234, Percent_Identity=39.7435897435897, Blast_Score=182, Evalue=4e-46,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_SHEAM (A1S4R2)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_927038.1
- ProteinModelPortal:   A1S4R2
- SMR:   A1S4R2
- STRING:   A1S4R2
- GeneID:   4603413
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_1161
- NMPDR:   fig|326297.7.peg.1093
- eggNOG:   COG0442
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- PhylomeDB:   A1S4R2
- ProtClustDB:   PRK09194
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.90.960.10
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 63362; Mature: 63362

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVSKYLLSTQKETPANAEVVSHQLMLRAGMIRRNASGLYSYLPTGLRVLRKVEAIVREE
CCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
MNKAGAIEILMPMVQPGDLWVETGRWDQFGPELLRFTDRHNRDFVLGPTHEEVITDLIRK
HCCCCCEEEEECCCCCCCEEEECCCCCCCCHHHHHHHCCCCCCEEECCCHHHHHHHHHHH
EVSSYKQLPLNLYQIQTKFRDEVRPRFGVMRSREFLMKDAYSFHLTQETLDETYQAMYTA
HHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHH
YSNIFSRMGLAFRPVLADTGSIGGSVSHEFHVLAQSGEDLIAYSTGSDYAANIEKAEAPL
HHHHHHHCCCCEEHHHHCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCHHCCCCC
PTEPRGEATEALRTVDTPNAKTIAELVEQFGVAIEKTIKTLIVKGATEEAPLVALLVRGD
CCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCEEEEEEECC
HELNEIKADKLELVASPLEFAGEAEIRAAVGAGTGSIGPVNLKMPIIADHSVLVMSDFAA
CHHHHHHHHHHHHHHCCHHHCCCCCEEEEECCCCCCCCCEEEEEEEEECCCEEEEECCCC
GANEDGKHFFGINWERDLPLVQGADIRNVVEGEATPDGKGTYAFARGIEVGHIFQLGNKY
CCCCCCCEEEECCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHCCCCHHHHHHHHHHH
SEAMNATVLDENGKSQIMLMGCYGVGVSRIVAAAIEQNNDERGIVWPEAIAPFTVGILPM
HHHCCCEEEECCCCCEEEEEEECHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCEEEEEEE
NMHKSHRVTDTAEALYKELTEAGFEVLFDDRKERPGVMFADMELLGIPHTVVIGDRNIDT
CCCCCCCCCHHHHHHHHHHHHHHHHEEECCCCCCCCEEEECHHEECCCEEEEEECCCCCC
GVYEYKNRRTGEKTEVPFAELVSFLKAQFQQG
CHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRVSKYLLSTQKETPANAEVVSHQLMLRAGMIRRNASGLYSYLPTGLRVLRKVEAIVREE
CCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
MNKAGAIEILMPMVQPGDLWVETGRWDQFGPELLRFTDRHNRDFVLGPTHEEVITDLIRK
HCCCCCEEEEECCCCCCCEEEECCCCCCCCHHHHHHHCCCCCCEEECCCHHHHHHHHHHH
EVSSYKQLPLNLYQIQTKFRDEVRPRFGVMRSREFLMKDAYSFHLTQETLDETYQAMYTA
HHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHH
YSNIFSRMGLAFRPVLADTGSIGGSVSHEFHVLAQSGEDLIAYSTGSDYAANIEKAEAPL
HHHHHHHCCCCEEHHHHCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCHHCCCCC
PTEPRGEATEALRTVDTPNAKTIAELVEQFGVAIEKTIKTLIVKGATEEAPLVALLVRGD
CCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCEEEEEEECC
HELNEIKADKLELVASPLEFAGEAEIRAAVGAGTGSIGPVNLKMPIIADHSVLVMSDFAA
CHHHHHHHHHHHHHHCCHHHCCCCCEEEEECCCCCCCCCEEEEEEEEECCCEEEEECCCC
GANEDGKHFFGINWERDLPLVQGADIRNVVEGEATPDGKGTYAFARGIEVGHIFQLGNKY
CCCCCCCEEEECCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHCCCCHHHHHHHHHHH
SEAMNATVLDENGKSQIMLMGCYGVGVSRIVAAAIEQNNDERGIVWPEAIAPFTVGILPM
HHHCCCEEEECCCCCEEEEEEECHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCEEEEEEE
NMHKSHRVTDTAEALYKELTEAGFEVLFDDRKERPGVMFADMELLGIPHTVVIGDRNIDT
CCCCCCCCCHHHHHHHHHHHHHHHHEEECCCCCCCCEEEECHHEECCCEEEEEECCCCCC
GVYEYKNRRTGEKTEVPFAELVSFLKAQFQQG
CHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA