Click here to switch to the map view.

The map label for this gene is tsf

Identifier: 119774277

GI number: 119774277

Start: 1390156

End: 1391007

Strand: Direct

Name: tsf

Synonym: Sama_1140

Alternate gene names: 119774277

Gene position: 1390156-1391007 (Clockwise)

Preceding gene: 119774276

Following gene: 119774278

Centisome position: 32.28

GC content: 50.35

Gene sequence:

>852_bases
ATGGCAATTTCTGCTGCCCTGGTTAAAGAACTGCGTGAGCGCACTGGCGCCGGCATGATGGATTGTAAAAAGGCTCTGGA
AGAGACTAACGGTGATATCGAGCTTGCTATCGATAACATGCGTAAGAGTGGCGCTGCCAAAGCTGCCAAGAAAGCCGGTA
ACATCGCTGCTGAAGGTACCATCCTGATCAAGCAAGGTGCTGGTTTCACTGCTCTGCTGGAAGTTAACTGCCAAACTGAC
TTCGTAGCCAAAGATTCTAACTTCCTGGCTTTCGCTAACCAGGTTCTGGAAGTGGCTGCTGCCGGTAAAGTATCCGTTGA
AGACCTGAAAGCACAGTTTGAAGAAGCTCGTGTTGCTCTGGTTGCCAAAATCGGTGAAAACATCAACGTACGTCGCGTTG
AGTACATCGACGGTGAAAACGTGGCTGCTTACCGCCACGGCGACCGTATCGGTGTAGTAGTTACCGGTACCGCTGACGAA
GAAACTCTGAAGCACCTGGCCATGCACGTTGCTGCATCCAAGCCTGAGTACGTAAATCCAGAAGACGTACCAGCTGAAGT
GGTTGCCAAAGAGCGTGAAGTTCAGGTTGAAATCGCCATCAACGAAGGCAAGCCAAAAGAAATCGCCGAGAAGATGGTTG
AAGGCCGTATGAAGAAGTTCACCGGTGAGGTTTCTCTGACCGGTCAGCCATTCATCATGGAGCCAAAGAAGACTGTTGGC
GAATTCCTGAAAGAGAAAGGCGCTACTGTTGCTAACTTCATCCGCCTGGAAGTGGGTGAAGGTATCGAGAAGAAAGAAGA
AGATTTCGCGGCCGAAGTGGCCAAGCAAATCGCTGCTTCTCAAAAGGCCTAA

Upstream 100 bases:

>100_bases
TTAACCAAGATCTATGATTGGTTAACAGGGGCCCGTGGGCCCCTGTTTTATACCTGCTTACTCGGCAAATGATTGCCGAA
ACTCAAAGAGGAATTTAACA

Downstream 100 bases:

>100_bases
TCGCCGCTAGGTAAGTACCCCAAGACCGCAGCAGCCGCTGCGGTCTTGTTATAATCAGGATAAAAATCATGAGCACCAAT
CCAAAACCAGCATTTCGACG

Product: elongation factor Ts

Products: NA

Alternate protein names: EF-Ts

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MAISAALVKELRERTGAGMMDCKKALEETNGDIELAIDNMRKSGAAKAAKKAGNIAAEGTILIKQGAGFTALLEVNCQTD
FVAKDSNFLAFANQVLEVAAAGKVSVEDLKAQFEEARVALVAKIGENINVRRVEYIDGENVAAYRHGDRIGVVVTGTADE
ETLKHLAMHVAASKPEYVNPEDVPAEVVAKEREVQVEIAINEGKPKEIAEKMVEGRMKKFTGEVSLTGQPFIMEPKKTVG
EFLKEKGATVANFIRLEVGEGIEKKEEDFAAEVAKQIAASQKA

Sequences:

>Translated_283_residues
MAISAALVKELRERTGAGMMDCKKALEETNGDIELAIDNMRKSGAAKAAKKAGNIAAEGTILIKQGAGFTALLEVNCQTD
FVAKDSNFLAFANQVLEVAAAGKVSVEDLKAQFEEARVALVAKIGENINVRRVEYIDGENVAAYRHGDRIGVVVTGTADE
ETLKHLAMHVAASKPEYVNPEDVPAEVVAKEREVQVEIAINEGKPKEIAEKMVEGRMKKFTGEVSLTGQPFIMEPKKTVG
EFLKEKGATVANFIRLEVGEGIEKKEEDFAAEVAKQIAASQKA
>Mature_282_residues
AISAALVKELRERTGAGMMDCKKALEETNGDIELAIDNMRKSGAAKAAKKAGNIAAEGTILIKQGAGFTALLEVNCQTDF
VAKDSNFLAFANQVLEVAAAGKVSVEDLKAQFEEARVALVAKIGENINVRRVEYIDGENVAAYRHGDRIGVVVTGTADEE
TLKHLAMHVAASKPEYVNPEDVPAEVVAKEREVQVEIAINEGKPKEIAEKMVEGRMKKFTGEVSLTGQPFIMEPKKTVGE
FLKEKGATVANFIRLEVGEGIEKKEEDFAAEVAKQIAASQKA

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family

Homologues:

Organism=Homo sapiens, GI171846268, Length=292, Percent_Identity=26.027397260274, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI291084500, Length=313, Percent_Identity=24.6006389776358, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI291084498, Length=115, Percent_Identity=39.1304347826087, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI291084502, Length=115, Percent_Identity=39.1304347826087, Blast_Score=68, Evalue=9e-12,
Organism=Escherichia coli, GI1786366, Length=274, Percent_Identity=72.2627737226277, Blast_Score=390, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17561440, Length=292, Percent_Identity=23.972602739726, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): EFTS_SHEAM (A1S4P1)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_927017.1
- ProteinModelPortal:   A1S4P1
- SMR:   A1S4P1
- STRING:   A1S4P1
- GeneID:   4603392
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_1140
- NMPDR:   fig|326297.7.peg.1072
- eggNOG:   COG0264
- HOGENOM:   HBG713289
- OMA:   YLHGTRI
- PhylomeDB:   A1S4P1
- ProtClustDB:   PRK09377
- GO:   GO:0005737
- HAMAP:   MF_00050
- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- InterPro:   IPR000449
- Gene3D:   G3DSA:3.30.479.20
- PANTHER:   PTHR11741
- TIGRFAMs:   TIGR00116

Pfam domain/function: PF00889 EF_TS; PF00627 UBA; SSF54713 EF_TS; SSF46934 UBA_like

EC number: NA

Molecular weight: Translated: 30489; Mature: 30358

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS01126 EF_TS_1; PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAISAALVKELRERTGAGMMDCKKALEETNGDIELAIDNMRKSGAAKAAKKAGNIAAEGT
CCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECHHHCCHHHHHHHCCCEEECCE
ILIKQGAGFTALLEVNCQTDFVAKDSNFLAFANQVLEVAAAGKVSVEDLKAQFEEARVAL
EEEECCCCEEEEEEECCCCCEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
VAKIGENINVRRVEYIDGENVAAYRHGDRIGVVVTGTADEETLKHLAMHVAASKPEYVNP
HHHHCCCCCEEEEEEECCCCEEEEECCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCC
EDVPAEVVAKEREVQVEIAINEGKPKEIAEKMVEGRMKKFTGEVSLTGQPFIMEPKKTVG
CCCCHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEECCCCEEECCHHHHH
EFLKEKGATVANFIRLEVGEGIEKKEEDFAAEVAKQIAASQKA
HHHHHCCCHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AISAALVKELRERTGAGMMDCKKALEETNGDIELAIDNMRKSGAAKAAKKAGNIAAEGT
CCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECHHHCCHHHHHHHCCCEEECCE
ILIKQGAGFTALLEVNCQTDFVAKDSNFLAFANQVLEVAAAGKVSVEDLKAQFEEARVAL
EEEECCCCEEEEEEECCCCCEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
VAKIGENINVRRVEYIDGENVAAYRHGDRIGVVVTGTADEETLKHLAMHVAASKPEYVNP
HHHHCCCCCEEEEEEECCCCEEEEECCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCC
EDVPAEVVAKEREVQVEIAINEGKPKEIAEKMVEGRMKKFTGEVSLTGQPFIMEPKKTVG
CCCCHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEECCCCEEECCHHHHH
EFLKEKGATVANFIRLEVGEGIEKKEEDFAAEVAKQIAASQKA
HHHHHCCCHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA