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The map label for this gene is gshA

Identifier: 119774195

GI number: 119774195

Start: 1281529

End: 1283109

Strand: Direct

Name: gshA

Synonym: Sama_1058

Alternate gene names: 119774195

Gene position: 1281529-1283109 (Clockwise)

Preceding gene: 119774194

Following gene: 119774196

Centisome position: 29.76

GC content: 55.34

Gene sequence:

>1581_bases
TTGACAGCATTCAATGACAGACTCGGGGCTCTCTCCGATGCAGAAGGACGCAAGGCACTTGCCGGGCTGCGACGGGGTTT
GGAGCGTGAGGCTTTGCGTATCACCAGCTGTTGCCAGCTGGCCCTGGATCCCCATCCCAAAGCGCTTGGGTCGGCGCTGA
CCCACTCCAGGATCACCACTGATTACAGTGAGGCCTTGCTGGAGTTTATTACCCCGGTCAGTGGCAACATTGAAGACTTG
CTGGAAGGCCTGACTGAAACCCACGCCTATACCCTGAAACATTTGGATGGCCAGAAGCTGTGGCCGGTGAGCATGCCCTG
CTATGTTGGCGATGTGAAAGACATACCCATTGCCCAATATGGCACCTCCAATACCGGCAGGATGAAAACCCTCTATCGCA
AGGGGCTGACCTACAGATACGGCGCCCTGATGCAGATTATTTCCGGGGTGCATTTTAACTTTTCGCTCTCCAGCGACTTG
TGGCCAAGATTGCATGCACTCTCGGGCAGCAGCCTGTCGCTGGACGAGTTTATCTCCGAATCTTACTTTGGCCTTATCCG
CAATTACCGCCGCCTGGTATGGGTATTGCCTTACCTTTTTGGCGCGTCACCGGCGATTTGTGGCTCTTTCCTCAAAGGCC
AGAAAACCAGTCTCGAGTTTGAAAAAACAGCTGGCGGCACCCTGTATCTGCCGTACGCGACCTCGCTGAGAATGAGTGAC
CTTGGCTACACCAACAAAGAGCAGGCCAGCCTTAACATCAGCTATGACTCTCTGCATGATTATTTGCAGGGGATCCGTGA
GGCGATTTGCTTGCCATCGGCCAAGTTTGCCGAAATTGGCGTGAAAGTGGATGGTGAATATCGCCAGCTCAATGCCAATG
TGCTCCAGATTGAAAACGAATTTTACGCCCCCATTCGAGCCAAGCGGGTGACCCGCAAGGGTGAAAAGCCATCTGAGGCG
CTGGCTCGTGCCGGGGTTGAGTACATTGAAGTGCGGGCGCTGGACGTAAATCCCTTCAGCCCGGTTGGGGTTGAAGCATC
ACAGCTGCGTTTCCTGGATGTCTTCCTGCTTTATTGCCTGTTGTCGGATTCGCCAAAGAGCGACGCGGTCTGTGAATCTG
AAATCACGTCCAACCTCAGAGCCGTTATCCATGAAGGGCGCAAACCCGGACTGGCACTCAGTCGTCAGGGCGAGCCTGTG
ACACTGAAAGCCTGGCTGCTGGAACTCTTCGATGAGTTTGATAAGCTCGCCAGTCTGCTGGACATAGACGGCAGCGACTA
TGCCGAAGCGCTGGCGCAATGGCGCATGGCCGTTGAAGACCCCGCCCTGACGCTTTCTGGCAGGGTACATGCCGCGGTTG
TGGAGCAGGGCATGAATCATGGCGATTTCGTGATGGGACTGGCGGCCAAATATCAGGATTTCTTCCTGTCGTATCCGCTG
TCTCCCGGCGTGGAAGCGGGTTTCCAGGCCGAGGCGGCATCCTCCCTTGCCGCACAGGCCGAGATTGAAGCCAGTGATGA
AGAAAGCTTTGATGAGTATCTCAAGGGCTACTTCAAAGGTGTGCCATGCGCGCTGTCCTGA

Upstream 100 bases:

>100_bases
AATGGCATTATTTTGGACAAAAGCCGAAAGACCCGAGGGTCGCCACAGGTCTTGCTGTGTACGGCGGCGCAGCGCGCCAT
AGCAACCAGAGAAGAATCAA

Downstream 100 bases:

>100_bases
CCCTGACACTCTGCGCAGGACTGACGGCGTGCGCCAGCAGCGTTGACGACGCCAATCAGTGCGGCTGGGTCTCTGGCTAT
CAGGACCCGCCGGAGACAGA

Product: glutamate--cysteine ligase

Products: NA

Alternate protein names: Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MTAFNDRLGALSDAEGRKALAGLRRGLEREALRITSCCQLALDPHPKALGSALTHSRITTDYSEALLEFITPVSGNIEDL
LEGLTETHAYTLKHLDGQKLWPVSMPCYVGDVKDIPIAQYGTSNTGRMKTLYRKGLTYRYGALMQIISGVHFNFSLSSDL
WPRLHALSGSSLSLDEFISESYFGLIRNYRRLVWVLPYLFGASPAICGSFLKGQKTSLEFEKTAGGTLYLPYATSLRMSD
LGYTNKEQASLNISYDSLHDYLQGIREAICLPSAKFAEIGVKVDGEYRQLNANVLQIENEFYAPIRAKRVTRKGEKPSEA
LARAGVEYIEVRALDVNPFSPVGVEASQLRFLDVFLLYCLLSDSPKSDAVCESEITSNLRAVIHEGRKPGLALSRQGEPV
TLKAWLLELFDEFDKLASLLDIDGSDYAEALAQWRMAVEDPALTLSGRVHAAVVEQGMNHGDFVMGLAAKYQDFFLSYPL
SPGVEAGFQAEAASSLAAQAEIEASDEESFDEYLKGYFKGVPCALS

Sequences:

>Translated_526_residues
MTAFNDRLGALSDAEGRKALAGLRRGLEREALRITSCCQLALDPHPKALGSALTHSRITTDYSEALLEFITPVSGNIEDL
LEGLTETHAYTLKHLDGQKLWPVSMPCYVGDVKDIPIAQYGTSNTGRMKTLYRKGLTYRYGALMQIISGVHFNFSLSSDL
WPRLHALSGSSLSLDEFISESYFGLIRNYRRLVWVLPYLFGASPAICGSFLKGQKTSLEFEKTAGGTLYLPYATSLRMSD
LGYTNKEQASLNISYDSLHDYLQGIREAICLPSAKFAEIGVKVDGEYRQLNANVLQIENEFYAPIRAKRVTRKGEKPSEA
LARAGVEYIEVRALDVNPFSPVGVEASQLRFLDVFLLYCLLSDSPKSDAVCESEITSNLRAVIHEGRKPGLALSRQGEPV
TLKAWLLELFDEFDKLASLLDIDGSDYAEALAQWRMAVEDPALTLSGRVHAAVVEQGMNHGDFVMGLAAKYQDFFLSYPL
SPGVEAGFQAEAASSLAAQAEIEASDEESFDEYLKGYFKGVPCALS
>Mature_525_residues
TAFNDRLGALSDAEGRKALAGLRRGLEREALRITSCCQLALDPHPKALGSALTHSRITTDYSEALLEFITPVSGNIEDLL
EGLTETHAYTLKHLDGQKLWPVSMPCYVGDVKDIPIAQYGTSNTGRMKTLYRKGLTYRYGALMQIISGVHFNFSLSSDLW
PRLHALSGSSLSLDEFISESYFGLIRNYRRLVWVLPYLFGASPAICGSFLKGQKTSLEFEKTAGGTLYLPYATSLRMSDL
GYTNKEQASLNISYDSLHDYLQGIREAICLPSAKFAEIGVKVDGEYRQLNANVLQIENEFYAPIRAKRVTRKGEKPSEAL
ARAGVEYIEVRALDVNPFSPVGVEASQLRFLDVFLLYCLLSDSPKSDAVCESEITSNLRAVIHEGRKPGLALSRQGEPVT
LKAWLLELFDEFDKLASLLDIDGSDYAEALAQWRMAVEDPALTLSGRVHAAVVEQGMNHGDFVMGLAAKYQDFFLSYPLS
PGVEAGFQAEAASSLAAQAEIEASDEESFDEYLKGYFKGVPCALS

Specific function: Glutathione biosynthesis; first step. [C]

COG id: COG2918

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1789044, Length=505, Percent_Identity=50.2970297029703, Blast_Score=491, Evalue=1e-140,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSH1_SHEAM (A1S4F9)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_926935.1
- ProteinModelPortal:   A1S4F9
- SMR:   A1S4F9
- STRING:   A1S4F9
- GeneID:   4603310
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_1058
- NMPDR:   fig|326297.7.peg.997
- eggNOG:   COG2918
- HOGENOM:   HBG289023
- OMA:   FGLIRNY
- PhylomeDB:   A1S4F9
- ProtClustDB:   PRK02107
- HAMAP:   MF_00578
- InterPro:   IPR007370
- InterPro:   IPR006334
- TIGRFAMs:   TIGR01434

Pfam domain/function: PF04262 Glu_cys_ligase

EC number: =6.3.2.2

Molecular weight: Translated: 57965; Mature: 57834

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAFNDRLGALSDAEGRKALAGLRRGLEREALRITSCCQLALDPHPKALGSALTHSRITT
CCCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCC
DYSEALLEFITPVSGNIEDLLEGLTETHAYTLKHLDGQKLWPVSMPCYVGDVKDIPIAQY
CHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCEEECCCCCCCCCCC
GTSNTGRMKTLYRKGLTYRYGALMQIISGVHFNFSLSSDLWPRLHALSGSSLSLDEFISE
CCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCCCCCHHHHHCCCCCCCHHHHHHH
SYFGLIRNYRRLVWVLPYLFGASPAICGSFLKGQKTSLEFEKTAGGTLYLPYATSLRMSD
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEECCCCCEEEECCCCCEEHHH
LGYTNKEQASLNISYDSLHDYLQGIREAICLPSAKFAEIGVKVDGEYRQLNANVLQIENE
CCCCCCCCCEEECCHHHHHHHHHHHHHHHHCCCCCHHHCCEEECCCHHHHCCEEEEECCC
FYAPIRAKRVTRKGEKPSEALARAGVEYIEVRALDVNPFSPVGVEASQLRFLDVFLLYCL
CCCCHHHHHHHHCCCCHHHHHHHCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHH
LSDSPKSDAVCESEITSNLRAVIHEGRKPGLALSRQGEPVTLKAWLLELFDEFDKLASLL
HCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHH
DIDGSDYAEALAQWRMAVEDPALTLSGRVHAAVVEQGMNHGDFVMGLAAKYQDFFLSYPL
CCCCCHHHHHHHHHHHHHCCCCEEECCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCC
SPGVEAGFQAEAASSLAAQAEIEASDEESFDEYLKGYFKGVPCALS
CCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TAFNDRLGALSDAEGRKALAGLRRGLEREALRITSCCQLALDPHPKALGSALTHSRITT
CCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCC
DYSEALLEFITPVSGNIEDLLEGLTETHAYTLKHLDGQKLWPVSMPCYVGDVKDIPIAQY
CHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCEEECCCCCCCCCCC
GTSNTGRMKTLYRKGLTYRYGALMQIISGVHFNFSLSSDLWPRLHALSGSSLSLDEFISE
CCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCCCCCHHHHHCCCCCCCHHHHHHH
SYFGLIRNYRRLVWVLPYLFGASPAICGSFLKGQKTSLEFEKTAGGTLYLPYATSLRMSD
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEECCCCCEEEECCCCCEEHHH
LGYTNKEQASLNISYDSLHDYLQGIREAICLPSAKFAEIGVKVDGEYRQLNANVLQIENE
CCCCCCCCCEEECCHHHHHHHHHHHHHHHHCCCCCHHHCCEEECCCHHHHCCEEEEECCC
FYAPIRAKRVTRKGEKPSEALARAGVEYIEVRALDVNPFSPVGVEASQLRFLDVFLLYCL
CCCCHHHHHHHHCCCCHHHHHHHCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHH
LSDSPKSDAVCESEITSNLRAVIHEGRKPGLALSRQGEPVTLKAWLLELFDEFDKLASLL
HCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHH
DIDGSDYAEALAQWRMAVEDPALTLSGRVHAAVVEQGMNHGDFVMGLAAKYQDFFLSYPL
CCCCCHHHHHHHHHHHHHCCCCEEECCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCC
SPGVEAGFQAEAASSLAAQAEIEASDEESFDEYLKGYFKGVPCALS
CCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA