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The map label for this gene is mdtN [H]

Identifier: 119774137

GI number: 119774137

Start: 1211970

End: 1213034

Strand: Direct

Name: mdtN [H]

Synonym: Sama_1000

Alternate gene names: 119774137

Gene position: 1211970-1213034 (Clockwise)

Preceding gene: 119774136

Following gene: 119774138

Centisome position: 28.15

GC content: 57.75

Gene sequence:

>1065_bases
ATGCCTCAGTCCGCCAACACATCCAAAAAAATCAGTACTTACCTCTTTGTAACCGTTCTTGGCATCTGGCTTTACAGCCT
CTGGGCCGACCGGGTGACACCCATGACGACCCAGGCGTTTGTACACGCTTATTTGGTGCGTATTACCCCTGAAGTCGCGG
GAAACATAGTCCAGGTGAATGTGGACAACGATAAGCGGGTTGAGGCCGGTGAACTCCTGTTTGAGATAGATGCAACCCAG
TATCAGATTGCCCGCGAAAAAGCCCAGGCCGATTTGGAACGGGTGGGGCAAAGTCTGGGTGCCAGCACCGCTGCAGTGCA
GGTTGCCCAGGCCAAGGTGGGTGATGCCCGCGCCGCGCTGGATAACGCCAAGGCTCAGGCTACCCGGGTTGAAACCCTCG
CCGAGCGGGGCGTGCTCAGCCAGGCAGAGCTGGATAATGCAAGGGAAGCTGCCCAGCGTGCCCAAGCCAGTCTGGAGGCT
GCGCAGGCGTCTTTATTACAGGCCAAACAAAATCTGGGGCCTGTAGGGGAAAATAATCCAGAACTCAAAGCCGCCATGGC
AGCTCTGGCCAAGGCCGAACTGGATCTTAAGCGCACCCGGGTGGTGGCACCCTCCCGGGGTGTGGTCACCAATGTGCAGC
TGTCACCGGGTCAATACATGGCGCCCGGACAGCCGGCGCTGACGTTTATCGATCCCCGTGAGGTCTGGATTTCAGCCATG
ATGCGGGAAAACTCCCTTGAGCATATTCGCGAAGGCGGAAAGGTTGGTCTGGTATTCGATGCCTTGCCCGGACGGGTATT
TGAAGGCGTGGTGGACAGCATCGGCTGGGGCAGTGGGGGGGCCAACAACATCGACCAGACTACGGGCTTTGAAACCGCCC
AAACCGGCAAGCAACAAGCAAAGCGATTCCCTGTACATATCCGTTTCGACACGGCGCCCATGGCCAACGAGCTGCGTTTT
GGCTCTCAGGTGACAGTGGCGTTTTACACCGGTGAAAGCAGTGTGGGAGAGACCCTTGCCCAACTGTGGCTGTGGTGCTG
GAGTAAGCTGACTTATGTCTCCTGA

Upstream 100 bases:

>100_bases
GCGTTTTTCTTTTGCTTATATAGCCTTAACGCGGTCTTGTCGGTGGCGAGAAAAGCGCAGGCGCGCTAGCATAACGTCAT
TAATCGGAGGGAAAGCCGCT

Downstream 100 bases:

>100_bases
ACGCACCGAGGGACTTCGCCTTTGGTATTTCAAAGGCTTTTCCAGCAGGGCTAACCCAATCCTGAGAATCGCCGTAGCTG
CGCTGGTACCGCTGTTTGTA

Product: multidrug resistance efflux pump

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MPQSANTSKKISTYLFVTVLGIWLYSLWADRVTPMTTQAFVHAYLVRITPEVAGNIVQVNVDNDKRVEAGELLFEIDATQ
YQIAREKAQADLERVGQSLGASTAAVQVAQAKVGDARAALDNAKAQATRVETLAERGVLSQAELDNAREAAQRAQASLEA
AQASLLQAKQNLGPVGENNPELKAAMAALAKAELDLKRTRVVAPSRGVVTNVQLSPGQYMAPGQPALTFIDPREVWISAM
MRENSLEHIREGGKVGLVFDALPGRVFEGVVDSIGWGSGGANNIDQTTGFETAQTGKQQAKRFPVHIRFDTAPMANELRF
GSQVTVAFYTGESSVGETLAQLWLWCWSKLTYVS

Sequences:

>Translated_354_residues
MPQSANTSKKISTYLFVTVLGIWLYSLWADRVTPMTTQAFVHAYLVRITPEVAGNIVQVNVDNDKRVEAGELLFEIDATQ
YQIAREKAQADLERVGQSLGASTAAVQVAQAKVGDARAALDNAKAQATRVETLAERGVLSQAELDNAREAAQRAQASLEA
AQASLLQAKQNLGPVGENNPELKAAMAALAKAELDLKRTRVVAPSRGVVTNVQLSPGQYMAPGQPALTFIDPREVWISAM
MRENSLEHIREGGKVGLVFDALPGRVFEGVVDSIGWGSGGANNIDQTTGFETAQTGKQQAKRFPVHIRFDTAPMANELRF
GSQVTVAFYTGESSVGETLAQLWLWCWSKLTYVS
>Mature_353_residues
PQSANTSKKISTYLFVTVLGIWLYSLWADRVTPMTTQAFVHAYLVRITPEVAGNIVQVNVDNDKRVEAGELLFEIDATQY
QIAREKAQADLERVGQSLGASTAAVQVAQAKVGDARAALDNAKAQATRVETLAERGVLSQAELDNAREAAQRAQASLEAA
QASLLQAKQNLGPVGENNPELKAAMAALAKAELDLKRTRVVAPSRGVVTNVQLSPGQYMAPGQPALTFIDPREVWISAMM
RENSLEHIREGGKVGLVFDALPGRVFEGVVDSIGWGSGGANNIDQTTGFETAQTGKQQAKRFPVHIRFDTAPMANELRFG
SQVTVAFYTGESSVGETLAQLWLWCWSKLTYVS

Specific function: Could be involved in resistance to puromycin, acriflavine and tetraphenylarsonium chloride [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790519, Length=317, Percent_Identity=30.9148264984227, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1790024, Length=263, Percent_Identity=31.1787072243346, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1790012, Length=291, Percent_Identity=31.9587628865979, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI87081951, Length=335, Percent_Identity=28.3582089552239, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1789637, Length=299, Percent_Identity=29.4314381270903, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1788711, Length=337, Percent_Identity=26.7062314540059, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789041, Length=335, Percent_Identity=26.865671641791, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1787013, Length=307, Percent_Identity=28.0130293159609, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1789900, Length=234, Percent_Identity=27.7777777777778, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 38320; Mature: 38189

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQSANTSKKISTYLFVTVLGIWLYSLWADRVTPMTTQAFVHAYLVRITPEVAGNIVQVN
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHCCCEEEEE
VDNDKRVEAGELLFEIDATQYQIAREKAQADLERVGQSLGASTAAVQVAQAKVGDARAAL
CCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCHHHHHH
DNAKAQATRVETLAERGVLSQAELDNAREAAQRAQASLEAAQASLLQAKQNLGPVGENNP
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
ELKAAMAALAKAELDLKRTRVVAPSRGVVTNVQLSPGQYMAPGQPALTFIDPREVWISAM
HHHHHHHHHHHHHHHHHHHEEECCCCCCEEEEEECCCCCCCCCCCCEEEECHHHHHHHHH
MRENSLEHIREGGKVGLVFDALPGRVFEGVVDSIGWGSGGANNIDQTTGFETAQTGKQQA
HHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHH
KRFPVHIRFDTAPMANELRFGSQVTVAFYTGESSVGETLAQLWLWCWSKLTYVS
HHCCEEEEECCCCCHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PQSANTSKKISTYLFVTVLGIWLYSLWADRVTPMTTQAFVHAYLVRITPEVAGNIVQVN
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHCCCEEEEE
VDNDKRVEAGELLFEIDATQYQIAREKAQADLERVGQSLGASTAAVQVAQAKVGDARAAL
CCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCHHHHHH
DNAKAQATRVETLAERGVLSQAELDNAREAAQRAQASLEAAQASLLQAKQNLGPVGENNP
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
ELKAAMAALAKAELDLKRTRVVAPSRGVVTNVQLSPGQYMAPGQPALTFIDPREVWISAM
HHHHHHHHHHHHHHHHHHHEEECCCCCCEEEEEECCCCCCCCCCCCEEEECHHHHHHHHH
MRENSLEHIREGGKVGLVFDALPGRVFEGVVDSIGWGSGGANNIDQTTGFETAQTGKQQA
HHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHH
KRFPVHIRFDTAPMANELRFGSQVTVAFYTGESSVGETLAQLWLWCWSKLTYVS
HHCCEEEEECCCCCHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]