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The map label for this gene is ispH [H]

Identifier: 119774064

GI number: 119774064

Start: 1120174

End: 1121115

Strand: Direct

Name: ispH [H]

Synonym: Sama_0927

Alternate gene names: 119774064

Gene position: 1120174-1121115 (Clockwise)

Preceding gene: 119774063

Following gene: 119774065

Centisome position: 26.01

GC content: 54.25

Gene sequence:

>942_bases
ATGACTGGACAGATGAACATTCTGCTCGCCAATCCACGCGGCTTTTGTGCCGGCGTAGATCGCGCTATCAGCATCGTTGA
GCGTGCTCTTGAGCTGTTCTCGCCACCTATATATGTGCGTCACGAAGTGGTGCATAACCGTTATGTGGTGCAAAACCTCA
AAGATCGTGGTGCTATCTTTGTTGAAGAGTTGGATCAGGTGCCGGACAACAGCATAGTGATCTTCTCTGCCCACGGGGTG
TCGCAGGCGGTGCGGGCCGAGGCCAAAAAGCGTGGTCTGAAGGTATTCGATGCCACCTGCCCATTGGTGACCAAGGTGCA
CCTGCAGGTAACCCGGGCCAGCCGCAAAGGGGTTGAATGCATCCTGATTGGCCATGAGGGTCATCCGGAAGTTGAAGGTA
CTATGGGCCAGTACGATAACCCTGAGGGTGGCGTTTATCTGATTGAGTCGGTGGAAGATGTCGAAGCGCTTCAGGTGAAA
GACCCGGAAAACCTGTGCTTCGTCACTCAAACTACATTGTCGGTTGACGATACGCTGGACATCATCGCGGCGCTGCAGGC
GAAGTTTCCGTCCATTGAGGGGCCACGCAAGGACGATATTTGTTACGCCACTCAAAATCGCCAGGATGCGGTGCGCAGTC
TGGCGACTCAGGCCAAGCTCTTTATCGTGGTGGGTTCGAAAAACAGCTCCAACTCCAACCGTTTGCGTGAGCTTGCCGAA
AAGTCGGGTGCACAGGCGTACTTGGTCGACAGTGCCGCCGATGTAAAGGCTGAGTGGTTTGCCAATGTAGATAAAGTGGC
TGTCACAGCCGGAGCATCAGCACCGGAAGTACTGGTAAAGCAGGTTATCGATGCAATTACCCATCTCGCGCCCAGTGTGG
TAACCGAGGTAGAGGGTCGTAAGGAAGACGTAGTTTTCGCCGTTCCGGTTGAACTCAGGTGA

Upstream 100 bases:

>100_bases
TTGTCCGTGATGTGGCTGGCGATTCTGTGACGGTCGATCTGAACCATCCTCTGGCCGGTCAGGCCGTGACCTTCGAGCTG
GAAGTGCTGGAGGAGCTCTG

Downstream 100 bases:

>100_bases
TTGTTTGCTAAGTTAATGTTAAAAAGAGGCCTAAGCCTCTTTTTTTATTGCCGGAAAGTTCTAGGTATAATGTTGTAGCT
TTTTAGATGCTATTGTTACT

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MTGQMNILLANPRGFCAGVDRAISIVERALELFSPPIYVRHEVVHNRYVVQNLKDRGAIFVEELDQVPDNSIVIFSAHGV
SQAVRAEAKKRGLKVFDATCPLVTKVHLQVTRASRKGVECILIGHEGHPEVEGTMGQYDNPEGGVYLIESVEDVEALQVK
DPENLCFVTQTTLSVDDTLDIIAALQAKFPSIEGPRKDDICYATQNRQDAVRSLATQAKLFIVVGSKNSSNSNRLRELAE
KSGAQAYLVDSAADVKAEWFANVDKVAVTAGASAPEVLVKQVIDAITHLAPSVVTEVEGRKEDVVFAVPVELR

Sequences:

>Translated_313_residues
MTGQMNILLANPRGFCAGVDRAISIVERALELFSPPIYVRHEVVHNRYVVQNLKDRGAIFVEELDQVPDNSIVIFSAHGV
SQAVRAEAKKRGLKVFDATCPLVTKVHLQVTRASRKGVECILIGHEGHPEVEGTMGQYDNPEGGVYLIESVEDVEALQVK
DPENLCFVTQTTLSVDDTLDIIAALQAKFPSIEGPRKDDICYATQNRQDAVRSLATQAKLFIVVGSKNSSNSNRLRELAE
KSGAQAYLVDSAADVKAEWFANVDKVAVTAGASAPEVLVKQVIDAITHLAPSVVTEVEGRKEDVVFAVPVELR
>Mature_312_residues
TGQMNILLANPRGFCAGVDRAISIVERALELFSPPIYVRHEVVHNRYVVQNLKDRGAIFVEELDQVPDNSIVIFSAHGVS
QAVRAEAKKRGLKVFDATCPLVTKVHLQVTRASRKGVECILIGHEGHPEVEGTMGQYDNPEGGVYLIESVEDVEALQVKD
PENLCFVTQTTLSVDDTLDIIAALQAKFPSIEGPRKDDICYATQNRQDAVRSLATQAKLFIVVGSKNSSNSNRLRELAEK
SGAQAYLVDSAADVKAEWFANVDKVAVTAGASAPEVLVKQVIDAITHLAPSVVTEVEGRKEDVVFAVPVELR

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family [H]

Homologues:

Organism=Escherichia coli, GI1786212, Length=309, Percent_Identity=67.3139158576052, Blast_Score=437, Evalue=1e-124,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003451 [H]

Pfam domain/function: PF02401 LYTB [H]

EC number: =1.17.1.2 [H]

Molecular weight: Translated: 34100; Mature: 33969

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGQMNILLANPRGFCAGVDRAISIVERALELFSPPIYVRHEVVHNRYVVQNLKDRGAIF
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCEE
VEELDQVPDNSIVIFSAHGVSQAVRAEAKKRGLKVFDATCPLVTKVHLQVTRASRKGVEC
EHHHHCCCCCCEEEEECCCHHHHHHHHHHHCCCEEEECCCCEEEEEEEEEEECCCCCCEE
ILIGHEGHPEVEGTMGQYDNPEGGVYLIESVEDVEALQVKDPENLCFVTQTTLSVDDTLD
EEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEECCCCCEEEEEEEEECCCHHHH
IIAALQAKFPSIEGPRKDDICYATQNRQDAVRSLATQAKLFIVVGSKNSSNSNRLRELAE
HHHHHHHCCCCCCCCCCCCEEEECCCHHHHHHHHHCCEEEEEEEECCCCCCHHHHHHHHH
KSGAQAYLVDSAADVKAEWFANVDKVAVTAGASAPEVLVKQVIDAITHLAPSVVTEVEGR
HCCCEEEEEECCCCCHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
KEDVVFAVPVELR
CCCEEEEEEEECC
>Mature Secondary Structure 
TGQMNILLANPRGFCAGVDRAISIVERALELFSPPIYVRHEVVHNRYVVQNLKDRGAIF
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCEE
VEELDQVPDNSIVIFSAHGVSQAVRAEAKKRGLKVFDATCPLVTKVHLQVTRASRKGVEC
EHHHHCCCCCCEEEEECCCHHHHHHHHHHHCCCEEEECCCCEEEEEEEEEEECCCCCCEE
ILIGHEGHPEVEGTMGQYDNPEGGVYLIESVEDVEALQVKDPENLCFVTQTTLSVDDTLD
EEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEECCCCCEEEEEEEEECCCHHHH
IIAALQAKFPSIEGPRKDDICYATQNRQDAVRSLATQAKLFIVVGSKNSSNSNRLRELAE
HHHHHHHCCCCCCCCCCCCEEEECCCHHHHHHHHHCCEEEEEEEECCCCCCHHHHHHHHH
KSGAQAYLVDSAADVKAEWFANVDKVAVTAGASAPEVLVKQVIDAITHLAPSVVTEVEGR
HCCCEEEEEECCCCCHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
KEDVVFAVPVELR
CCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]