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The map label for this gene is mpl [H]

Identifier: 119773906

GI number: 119773906

Start: 952789

End: 954165

Strand: Direct

Name: mpl [H]

Synonym: Sama_0769

Alternate gene names: 119773906

Gene position: 952789-954165 (Clockwise)

Preceding gene: 119773904

Following gene: 119773907

Centisome position: 22.13

GC content: 58.17

Gene sequence:

>1377_bases
ATGCACGTACACATTCTCGGGATTTGCGGCACCTTTATGGGTGGTCTGGCGCTGCTGGCGCGGGCCATGGGACACAAGGT
CACAGGATCGGATGCCAACGTGTATCCGCCCATGAGCACTCAGCTCGAAGAGCAGGGTATTGAACTCATTCAGGGTTTTG
ACCCTGTGCAGCTTGAAAGCGAACCGGATTTGGTGGTGATTGGCAATGCCATGAGCCGGGGCAATCCCTGCGTGGAAGCC
GTGCTCAACAAGGGCATGGCCTACACCTCCGGGCCACAATTTCTGCGCGATCACATACTGCCCGGTCGCTGGGTGCTGGC
GGTGGCCGGTACCCACGGTAAAACCTCCACCTCCAGCATGTTGGCGTGGGTATTGGAGCACTGCGGCTATGAGCCGGGGT
TCCTGATTGGTGGCGTACCACAAAACTTCGGCGTATCAGCCCGTTTGGGGAATTCCCCCTTCTTTGTGGTTGAGGCCGAC
GAGTACGATAGCGCCTTTTTCGATAAGCGTTCCAAGTTTGTTCACTATCAACCAAAAACCCTGGTTATCAATAACCTCGA
GTTTGACCACGCCGATATTTTCGACTCGCTTGCCGACATTCAACGCCAGTTTAGCCATGTCATCCGCACCGTGCCCGGCT
GTGGCAAGGTGATTTGGCCCAAAGGCAGCAAAGCGGTGGCCGAGGTTATCGAAAAGGGGCTGTGGAGCGAGCAGGAAACC
TTTAGCCATCAGGCGGACGCCGATTGGTCGGTGCGGCCGCTGGCAGCCGATGGCCATAAATTTGAAGTGTTGTTTGGCGG
CGAAGTTCAGGGCGTGCTCGACTGGCAGCTTATCGGCAACCACAACATGGAAAACGCGGTCATGGCCATTGCTGCGGCGC
GCCACGTGGGGGTCGCCCCTGCGGCGGCTATTGAGGCCCTCAGCGGTTTTATGCCGCCCAAACGCAGGCTGGAGCTGATT
GGTGAGGTAGGCGGTGTCAGCCTGTACGATGACTTTGCTCACCATCCAACGGCCATTGAAACCACTCTCAAGGGGCTCAG
GGCCAAGGTGGGCAATGCCCGTATTTTTGTGGTGCTCGAGCCCCGCTCAAACACCATGAAACGCGGTGTTCACAAGGACA
CCCTGGCCTTGTCTATGGCGCTGGCCGACGAGGCCTTCCTCTATCAGGCCGACAATATCGGCTGGGATATCCGTGATGCC
ATGGCCCAGGCCCCGCTGCCGGTAGATGTGGACTATGATATCGACAGCATAGTCGCCAAGGTGACCGCCAAAGTTCAGCG
CGGTGACCAGCTGGTGGTGATGAGCAACGGCGGCTTCGGGGGGATCCATGGCAAGCTGAAGGCGGCCATTGCCGCCAGAT
TTGCCGAGTCTGTCTGA

Upstream 100 bases:

>100_bases
ACCGAGCCAAGCTCGCCTTTGGGGGGAGGCGAAGGTACAATAGCCGCTGATTTTTCAATTCTTTTGGGTATGGTTTCCGT
ACCTGAATTGGCAGGAGCCT

Downstream 100 bases:

>100_bases
GCTGCTAAAGAGACGTGAATCAAGATGAATGAACACCAGCCCCTGATGCCAAAGGTAAACAGCATCAGCCTCGCCTGGAC
GGGCGCCTCCGGCGCGCCCT

Product: UDP-N-acetylmuramate

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MHVHILGICGTFMGGLALLARAMGHKVTGSDANVYPPMSTQLEEQGIELIQGFDPVQLESEPDLVVIGNAMSRGNPCVEA
VLNKGMAYTSGPQFLRDHILPGRWVLAVAGTHGKTSTSSMLAWVLEHCGYEPGFLIGGVPQNFGVSARLGNSPFFVVEAD
EYDSAFFDKRSKFVHYQPKTLVINNLEFDHADIFDSLADIQRQFSHVIRTVPGCGKVIWPKGSKAVAEVIEKGLWSEQET
FSHQADADWSVRPLAADGHKFEVLFGGEVQGVLDWQLIGNHNMENAVMAIAAARHVGVAPAAAIEALSGFMPPKRRLELI
GEVGGVSLYDDFAHHPTAIETTLKGLRAKVGNARIFVVLEPRSNTMKRGVHKDTLALSMALADEAFLYQADNIGWDIRDA
MAQAPLPVDVDYDIDSIVAKVTAKVQRGDQLVVMSNGGFGGIHGKLKAAIAARFAESV

Sequences:

>Translated_458_residues
MHVHILGICGTFMGGLALLARAMGHKVTGSDANVYPPMSTQLEEQGIELIQGFDPVQLESEPDLVVIGNAMSRGNPCVEA
VLNKGMAYTSGPQFLRDHILPGRWVLAVAGTHGKTSTSSMLAWVLEHCGYEPGFLIGGVPQNFGVSARLGNSPFFVVEAD
EYDSAFFDKRSKFVHYQPKTLVINNLEFDHADIFDSLADIQRQFSHVIRTVPGCGKVIWPKGSKAVAEVIEKGLWSEQET
FSHQADADWSVRPLAADGHKFEVLFGGEVQGVLDWQLIGNHNMENAVMAIAAARHVGVAPAAAIEALSGFMPPKRRLELI
GEVGGVSLYDDFAHHPTAIETTLKGLRAKVGNARIFVVLEPRSNTMKRGVHKDTLALSMALADEAFLYQADNIGWDIRDA
MAQAPLPVDVDYDIDSIVAKVTAKVQRGDQLVVMSNGGFGGIHGKLKAAIAARFAESV
>Mature_458_residues
MHVHILGICGTFMGGLALLARAMGHKVTGSDANVYPPMSTQLEEQGIELIQGFDPVQLESEPDLVVIGNAMSRGNPCVEA
VLNKGMAYTSGPQFLRDHILPGRWVLAVAGTHGKTSTSSMLAWVLEHCGYEPGFLIGGVPQNFGVSARLGNSPFFVVEAD
EYDSAFFDKRSKFVHYQPKTLVINNLEFDHADIFDSLADIQRQFSHVIRTVPGCGKVIWPKGSKAVAEVIEKGLWSEQET
FSHQADADWSVRPLAADGHKFEVLFGGEVQGVLDWQLIGNHNMENAVMAIAAARHVGVAPAAAIEALSGFMPPKRRLELI
GEVGGVSLYDDFAHHPTAIETTLKGLRAKVGNARIFVVLEPRSNTMKRGVHKDTLALSMALADEAFLYQADNIGWDIRDA
MAQAPLPVDVDYDIDSIVAKVTAKVQRGDQLVVMSNGGFGGIHGKLKAAIAARFAESV

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=454, Percent_Identity=61.4537444933921, Blast_Score=575, Evalue=1e-165,
Organism=Escherichia coli, GI1786279, Length=284, Percent_Identity=28.8732394366197, Blast_Score=121, Evalue=9e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 49549; Mature: 49549

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHVHILGICGTFMGGLALLARAMGHKVTGSDANVYPPMSTQLEEQGIELIQGFDPVQLES
CEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEECC
EPDLVVIGNAMSRGNPCVEAVLNKGMAYTSGPQFLRDHILPGRWVLAVAGTHGKTSTSSM
CCCEEEEECCCCCCCHHHHHHHHCCCCCCCCHHHHHHHCCCCCEEEEEECCCCCCCHHHH
LAWVLEHCGYEPGFLIGGVPQNFGVSARLGNSPFFVVEADEYDSAFFDKRSKFVHYQPKT
HHHHHHHCCCCCCEEECCCCCCCCCEEEECCCCEEEEECCCCCHHHHHCCCCEEEECCCE
LVINNLEFDHADIFDSLADIQRQFSHVIRTVPGCGKVIWPKGSKAVAEVIEKGLWSEQET
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHCCCCHHH
FSHQADADWSVRPLAADGHKFEVLFGGEVQGVLDWQLIGNHNMENAVMAIAAARHVGVAP
HCCCCCCCCCEEEEECCCCEEEEEECCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCH
AAAIEALSGFMPPKRRLELIGEVGGVSLYDDFAHHPTAIETTLKGLRAKVGNARIFVVLE
HHHHHHHHCCCCHHHHHHHHHHCCCEEEHHHHCCCCHHHHHHHHHHHHHCCCEEEEEEEC
PRSNTMKRGVHKDTLALSMALADEAFLYQADNIGWDIRDAMAQAPLPVDVDYDIDSIVAK
CCCCHHHCCCCHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHCCCCCEECCCCHHHHHHH
VTAKVQRGDQLVVMSNGGFGGIHGKLKAAIAARFAESV
HHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHVHILGICGTFMGGLALLARAMGHKVTGSDANVYPPMSTQLEEQGIELIQGFDPVQLES
CEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEECC
EPDLVVIGNAMSRGNPCVEAVLNKGMAYTSGPQFLRDHILPGRWVLAVAGTHGKTSTSSM
CCCEEEEECCCCCCCHHHHHHHHCCCCCCCCHHHHHHHCCCCCEEEEEECCCCCCCHHHH
LAWVLEHCGYEPGFLIGGVPQNFGVSARLGNSPFFVVEADEYDSAFFDKRSKFVHYQPKT
HHHHHHHCCCCCCEEECCCCCCCCCEEEECCCCEEEEECCCCCHHHHHCCCCEEEECCCE
LVINNLEFDHADIFDSLADIQRQFSHVIRTVPGCGKVIWPKGSKAVAEVIEKGLWSEQET
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHCCCCHHH
FSHQADADWSVRPLAADGHKFEVLFGGEVQGVLDWQLIGNHNMENAVMAIAAARHVGVAP
HCCCCCCCCCEEEEECCCCEEEEEECCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCH
AAAIEALSGFMPPKRRLELIGEVGGVSLYDDFAHHPTAIETTLKGLRAKVGNARIFVVLE
HHHHHHHHCCCCHHHHHHHHHHCCCEEEHHHHCCCCHHHHHHHHHHHHHCCCEEEEEEEC
PRSNTMKRGVHKDTLALSMALADEAFLYQADNIGWDIRDAMAQAPLPVDVDYDIDSIVAK
CCCCHHHCCCCHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHCCCCCEECCCCHHHHHHH
VTAKVQRGDQLVVMSNGGFGGIHGKLKAAIAARFAESV
HHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]