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The map label for this gene is cobS

Identifier: 119773900

GI number: 119773900

Start: 946177

End: 946959

Strand: Direct

Name: cobS

Synonym: Sama_0763

Alternate gene names: 119773900

Gene position: 946177-946959 (Clockwise)

Preceding gene: 119773899

Following gene: 119773901

Centisome position: 21.97

GC content: 55.3

Gene sequence:

>783_bases
ATGTCGACCTTTAAGCGTGAACTTGAGTTGTTTTTGTTGGCGCTCACCTTTTTTACCCGCATTCCTGTGCCGGTAAAACT
CGATTACAGCGGCGAAGGGTTAAACCGTGCCAGTCGTTACTTTGGCCTGGTGGGCACTCTGGTGGGGTTGATTGCTGCCC
TGGTGTTTTACCTGACCCAGTTTATCTTTCCTGCCTCGGTGGCCGTGGTGCTGGCCATGATTGCCACCGTCTTGCTCACC
GGCGGTTTCCATGAAGATGGCCTTGCCGATACCGCAGACGGATTTGGCGGTGCCTTCGAGCGGGAACGTAAGCTCGAAAT
CATGAAAGACAGCCGGGTCGGTTCCTATGGTTCACTGGCGCTGATGTTGGCGTTGCTGCTGAAGTTTCAGCTGTTAAGCG
AACTGGCACTCTATTCAGTTTCAAGTGTTGCCGGTGGCCTGGTGTTGGGGCATACCTTAAGCCGTGCCTTCGCCGCCAGT
ATCATTTTCAACCATACCTATGTGCGAGAAGATGCAGAGAGCAAAGCCAAGCCGCTGGCTCAGTCGATGCACTGGGATGA
AGTGCTGTTTTTAGTGCTCAGCGCCGGGGTGATTTGCCTGCTGCTCACCGGTGTGGGCGCAACCCTGGTGATACTCGTTA
CCCTCTTTGTGGCCCGCAGCCTGCTTGCCCGTTGGCAGTCACGCCATATCGGCGGCTATACGGGCGACACGCTTGGTGCC
TGTCAGCAGATTCTCGAACTGGTGGTCTATCTGGTGCTCTTACTGCTCTGGAGCCAGTCATGA

Upstream 100 bases:

>100_bases
GCCTTGCCACTGGTGCAGGCAGCGGCCAATTTTTATCGTGAGATGGCGAGCTTCAGCGACGCCGGAATTACAGATGTAAC
GCCTTAGGGCGGGAGCAGTT

Downstream 100 bases:

>100_bases
TCCATCTGGTTCTCGGTGGTGCCCGCAGTGGCAAGAGTCGTTTTGCCGAGACCCAGATTGCCCGGGCTTCACTTCAGGGC
ATTTATCTTGCCACGGCCAC

Product: cobalamin 5'-phosphate synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MSTFKRELELFLLALTFFTRIPVPVKLDYSGEGLNRASRYFGLVGTLVGLIAALVFYLTQFIFPASVAVVLAMIATVLLT
GGFHEDGLADTADGFGGAFERERKLEIMKDSRVGSYGSLALMLALLLKFQLLSELALYSVSSVAGGLVLGHTLSRAFAAS
IIFNHTYVREDAESKAKPLAQSMHWDEVLFLVLSAGVICLLLTGVGATLVILVTLFVARSLLARWQSRHIGGYTGDTLGA
CQQILELVVYLVLLLLWSQS

Sequences:

>Translated_260_residues
MSTFKRELELFLLALTFFTRIPVPVKLDYSGEGLNRASRYFGLVGTLVGLIAALVFYLTQFIFPASVAVVLAMIATVLLT
GGFHEDGLADTADGFGGAFERERKLEIMKDSRVGSYGSLALMLALLLKFQLLSELALYSVSSVAGGLVLGHTLSRAFAAS
IIFNHTYVREDAESKAKPLAQSMHWDEVLFLVLSAGVICLLLTGVGATLVILVTLFVARSLLARWQSRHIGGYTGDTLGA
CQQILELVVYLVLLLLWSQS
>Mature_259_residues
STFKRELELFLLALTFFTRIPVPVKLDYSGEGLNRASRYFGLVGTLVGLIAALVFYLTQFIFPASVAVVLAMIATVLLTG
GFHEDGLADTADGFGGAFERERKLEIMKDSRVGSYGSLALMLALLLKFQLLSELALYSVSSVAGGLVLGHTLSRAFAASI
IFNHTYVREDAESKAKPLAQSMHWDEVLFLVLSAGVICLLLTGVGATLVILVTLFVARSLLARWQSRHIGGYTGDTLGAC
QQILELVVYLVLLLLWSQS

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin)

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family

Homologues:

Organism=Escherichia coli, GI1788301, Length=257, Percent_Identity=38.9105058365759, Blast_Score=140, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBS_SHEAM (A1S3L4)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_926640.1
- STRING:   A1S3L4
- GeneID:   4603015
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_0763
- NMPDR:   fig|326297.7.peg.716
- eggNOG:   COG0368
- HOGENOM:   HBG729392
- OMA:   HDSRIGA
- PhylomeDB:   A1S3L4
- HAMAP:   MF_00719
- InterPro:   IPR003805
- TIGRFAMs:   TIGR00317

Pfam domain/function: PF02654 CobS

EC number: 2.7.8.26

Molecular weight: Translated: 28241; Mature: 28109

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x113ed108)-; HASH(0x1190547c)-; HASH(0x10bf9ad8)-; HASH(0x1155a7a4)-; HASH(0x12349e5c)-; HASH(0x11c0f4f4)-;

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTFKRELELFLLALTFFTRIPVPVKLDYSGEGLNRASRYFGLVGTLVGLIAALVFYLTQ
CCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIFPASVAVVLAMIATVLLTGGFHEDGLADTADGFGGAFERERKLEIMKDSRVGSYGSLA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHH
LMLALLLKFQLLSELALYSVSSVAGGLVLGHTLSRAFAASIIFNHTYVREDAESKAKPLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSMHWDEVLFLVLSAGVICLLLTGVGATLVILVTLFVARSLLARWQSRHIGGYTGDTLGA
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
CQQILELVVYLVLLLLWSQS
HHHHHHHHHHHHHHHHHCCH
>Mature Secondary Structure 
STFKRELELFLLALTFFTRIPVPVKLDYSGEGLNRASRYFGLVGTLVGLIAALVFYLTQ
CHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIFPASVAVVLAMIATVLLTGGFHEDGLADTADGFGGAFERERKLEIMKDSRVGSYGSLA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHH
LMLALLLKFQLLSELALYSVSSVAGGLVLGHTLSRAFAASIIFNHTYVREDAESKAKPLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSMHWDEVLFLVLSAGVICLLLTGVGATLVILVTLFVARSLLARWQSRHIGGYTGDTLGA
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
CQQILELVVYLVLLLLWSQS
HHHHHHHHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA