Click here to switch to the map view.

The map label for this gene is pleD [H]

Identifier: 119773886

GI number: 119773886

Start: 923436

End: 924374

Strand: Direct

Name: pleD [H]

Synonym: Sama_0748

Alternate gene names: 119773886

Gene position: 923436-924374 (Clockwise)

Preceding gene: 119773885

Following gene: 119773889

Centisome position: 21.44

GC content: 51.97

Gene sequence:

>939_bases
ATGATAAATCAGGAGCAGCCATTTAAAGGCTCAGATGATGGACCGCCCAAGGTGCTGATAGTTGACGATAAGCCATCCAA
CATTCAGATAGTCCATCACATCATCAAAGACCAGTACCAGGTGTTGATGGCCACCTCAGGGAAACGTGCCATTGAAATAT
GCCTGGAGATGTTGCCTGACTTGGTGTTGATGGATGTCATCATGCCCGAGCTCAGTGGCCTCGATACCTGCAAGCTGATG
AAGCAGCATCCCGAACTGGTTAATATCCCGGTTATTTTTATTACTGGCCTGCAAGGTCAACATGATGAAAACGCCTGCTG
GGATGCCGGTGGGGTGGATTTTATTTCTAAGCCCTTTAACGCTACCACCCTTAAAAATCGCATCAAGGCCCAGGTGACCC
TGAAACGCCAGAGTGACTTATTGCGTCGGCTGGCGTTTTTGGATGGCCTGACCGGGGTGAAAAATCGTCATTTCTTCGAT
CAATATCTCGAGACTCAGCTGATGTTGGCAAGGCGCCAGTCTCAGGCTATCTGTGTGCTGATGGTGGATATCGACTATTT
CAAGCAGTACAACGACAGTTTTGGTCATCAGATGGGGGATGAGGCCTTGCGCTCTGTGGCACAGGCGCTGGGGGAAAGCT
GCAGGCGCCCGGCGGATTTGGTGGCACGCTATGGCGGTGAAGAGTTTGTGGTCGTACTGCCCAACACCCCAAAAAACGGG
GTAGAGCGGGTTATTGAACGTATTCAGGGGGCAATCGCCGAGCTGGATATCCACCACCCGGCATCGGCAACCGGCATTCT
GACGGTGAGCATGGGTGGCATGATTTGGCTTGCCGACTACCAAAGCACAGAGGCCCTGCTCGGTGAGGCCGATAAACTAT
TGTATCGTGCCAAAAGTCTGGGGCGAAACCGGGCATGTATTGATGCCAATATCCCTTGA

Upstream 100 bases:

>100_bases
ATGCAGCGCTGACCGGGAAGTTAGTGGATGCAGCAAATAACTTGGATTTTAATCAATCACTTGTTCTACTATCGGCTTTT
GAGGGATTGGAGAGGATAGG

Downstream 100 bases:

>100_bases
ACACCAATAAAAACGGCAGAATTGGATTCTGCCGTTTGTTTACCACCAAGACCCGTCATCCGGGGGAGTGGATGAGGTGC
TTATTGAGCCTGACTCTCAT

Product: response regulator receiver protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MINQEQPFKGSDDGPPKVLIVDDKPSNIQIVHHIIKDQYQVLMATSGKRAIEICLEMLPDLVLMDVIMPELSGLDTCKLM
KQHPELVNIPVIFITGLQGQHDENACWDAGGVDFISKPFNATTLKNRIKAQVTLKRQSDLLRRLAFLDGLTGVKNRHFFD
QYLETQLMLARRQSQAICVLMVDIDYFKQYNDSFGHQMGDEALRSVAQALGESCRRPADLVARYGGEEFVVVLPNTPKNG
VERVIERIQGAIAELDIHHPASATGILTVSMGGMIWLADYQSTEALLGEADKLLYRAKSLGRNRACIDANIP

Sequences:

>Translated_312_residues
MINQEQPFKGSDDGPPKVLIVDDKPSNIQIVHHIIKDQYQVLMATSGKRAIEICLEMLPDLVLMDVIMPELSGLDTCKLM
KQHPELVNIPVIFITGLQGQHDENACWDAGGVDFISKPFNATTLKNRIKAQVTLKRQSDLLRRLAFLDGLTGVKNRHFFD
QYLETQLMLARRQSQAICVLMVDIDYFKQYNDSFGHQMGDEALRSVAQALGESCRRPADLVARYGGEEFVVVLPNTPKNG
VERVIERIQGAIAELDIHHPASATGILTVSMGGMIWLADYQSTEALLGEADKLLYRAKSLGRNRACIDANIP
>Mature_312_residues
MINQEQPFKGSDDGPPKVLIVDDKPSNIQIVHHIIKDQYQVLMATSGKRAIEICLEMLPDLVLMDVIMPELSGLDTCKLM
KQHPELVNIPVIFITGLQGQHDENACWDAGGVDFISKPFNATTLKNRIKAQVTLKRQSDLLRRLAFLDGLTGVKNRHFFD
QYLETQLMLARRQSQAICVLMVDIDYFKQYNDSFGHQMGDEALRSVAQALGESCRRPADLVARYGGEEFVVVLPNTPKNG
VERVIERIQGAIAELDIHHPASATGILTVSMGGMIWLADYQSTEALLGEADKLLYRAKSLGRNRACIDANIP

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=185, Percent_Identity=34.5945945945946, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI87081881, Length=173, Percent_Identity=39.3063583815029, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1787262, Length=167, Percent_Identity=34.7305389221557, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1786584, Length=171, Percent_Identity=32.7485380116959, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788085, Length=209, Percent_Identity=28.2296650717703, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87082007, Length=171, Percent_Identity=35.0877192982456, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1787802, Length=189, Percent_Identity=33.8624338624339, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI87081977, Length=162, Percent_Identity=32.0987654320988, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1787541, Length=174, Percent_Identity=30.4597701149425, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787816, Length=158, Percent_Identity=32.9113924050633, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788381, Length=177, Percent_Identity=31.0734463276836, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1789149, Length=110, Percent_Identity=33.6363636363636, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081974, Length=106, Percent_Identity=35.8490566037736, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788956, Length=175, Percent_Identity=31.4285714285714, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 34785; Mature: 34785

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINQEQPFKGSDDGPPKVLIVDDKPSNIQIVHHIIKDQYQVLMATSGKRAIEICLEMLPD
CCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHH
LVLMDVIMPELSGLDTCKLMKQHPELVNIPVIFITGLQGQHDENACWDAGGVDFISKPFN
HHHHHHHHHHHCCCHHHHHHHHCCCEEECCEEEEECCCCCCCCCCCCCCCCCHHHCCCCC
ATTLKNRIKAQVTLKRQSDLLRRLAFLDGLTGVKNRHFFDQYLETQLMLARRQSQAICVL
HHHHHHHHHHEEEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEE
MVDIDYFKQYNDSFGHQMGDEALRSVAQALGESCRRPADLVARYGGEEFVVVLPNTPKNG
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEEECCCCHHH
VERVIERIQGAIAELDIHHPASATGILTVSMGGMIWLADYQSTEALLGEADKLLYRAKSL
HHHHHHHHHHHHHHEECCCCCCCCEEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHC
GRNRACIDANIP
CCCCEEEECCCC
>Mature Secondary Structure
MINQEQPFKGSDDGPPKVLIVDDKPSNIQIVHHIIKDQYQVLMATSGKRAIEICLEMLPD
CCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHH
LVLMDVIMPELSGLDTCKLMKQHPELVNIPVIFITGLQGQHDENACWDAGGVDFISKPFN
HHHHHHHHHHHCCCHHHHHHHHCCCEEECCEEEEECCCCCCCCCCCCCCCCCHHHCCCCC
ATTLKNRIKAQVTLKRQSDLLRRLAFLDGLTGVKNRHFFDQYLETQLMLARRQSQAICVL
HHHHHHHHHHEEEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEE
MVDIDYFKQYNDSFGHQMGDEALRSVAQALGESCRRPADLVARYGGEEFVVVLPNTPKNG
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEEECCCCHHH
VERVIERIQGAIAELDIHHPASATGILTVSMGGMIWLADYQSTEALLGEADKLLYRAKSL
HHHHHHHHHHHHHHEECCCCCCCCEEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHC
GRNRACIDANIP
CCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA