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The map label for this gene is lip2 [H]

Identifier: 119773665

GI number: 119773665

Start: 648743

End: 649699

Strand: Direct

Name: lip2 [H]

Synonym: Sama_0525

Alternate gene names: 119773665

Gene position: 648743-649699 (Clockwise)

Preceding gene: 119773664

Following gene: 119773669

Centisome position: 15.07

GC content: 55.8

Gene sequence:

>957_bases
ATGCAAGACTCTCCACGTGGCAAGGCGTCAGCAAAGCTGGCGGAGTTTCTGACCCAGGCCAATCACAACATTGCTCTGGT
GAGGGAGCAAAACATTCAGTTCACTCCGGCGCTGCTGAGGGAAAATCTGAATAAGCTCGCCGCCTTTATGACCTGTAAAC
CCGAGGTGAACTACATCGCCGACAAAGCCTGGCAACTGGATGGCCGAATTATAGCTGCGCGGGTATACAGTCCAGCGCCG
CACAGTGCCTTGCCTGTGGTGGTGCACTTTCACGGTGGTGGCCATATGTGCGGCAGCGTGGCGCTATACGACCCCATCTG
CCGCCAGTTGGCAAGCACAGCCCAATGTGTGGTGATAAGTGTTGAGTACCGTTTGGCGCCTGAATATCCCTATCCTGCGG
GGTTGGAGGATTGCGAGCATGCCCTGCGCCACTACGCCGAAGTGTTGGAGGATGTTGCCTTTATCGCCGGTCCCTGCATC
ATGGGTGACAGTGCAGGTGGCGCCATTTGCACCAGCCTCAGTGAGCGCAGTCTGGCGGATCCGACCCTCGATATTCGCAA
GCAAATTCTGATTTATCCCAGCGTGGATTACACCATGGCGAGCGCCTCTTACGAGAGCAACGGTGCAGGCTTCTTATTGG
AGACGCCAAGAGTGAAATGGTATTTCGAGCAATATTTTCAGGATGCAGCCCTGGATACTGAAAAGGTCAGAGCGGCGTCG
CCACTGCACGGCCGTATAGGATCAGGACTGCCTGAAACGCTGATTTTTACCGCTGGCTGCGATCCGCTGCGGGATGAAGG
CCGGGCCTATGCCGAGGCGTTACAGTGCGCGGGTGTAAAGGTAGAGCATCACGGGCTGGAGGATATGATCCACGCCTACA
TGCTGCTTCACGATCTGGTGGCCGAAGAGTGTTTGCGGACCTATGAGCACATTGCCCGCTTTATTAAGGCTGTTTGA

Upstream 100 bases:

>100_bases
GCAGCTATTTCGCCTCTTTTGTTCTTATTACTCTGCTGAGCGCCACCGTCTTTGTTGGCAGCTATTTTCTCCCTGAAACC
GGAAAACCCAAGGAGTAAGC

Downstream 100 bases:

>100_bases
CAGATGAGTCCGGTTCGGATGTCCCCAAATACTCCATCTGCAAGGCCTGGTATGTTTGGGTTTGTTTGAAATCGGCCATG
GCTTTATCAAATAGGGCTGT

Product: putative lipase

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MQDSPRGKASAKLAEFLTQANHNIALVREQNIQFTPALLRENLNKLAAFMTCKPEVNYIADKAWQLDGRIIAARVYSPAP
HSALPVVVHFHGGGHMCGSVALYDPICRQLASTAQCVVISVEYRLAPEYPYPAGLEDCEHALRHYAEVLEDVAFIAGPCI
MGDSAGGAICTSLSERSLADPTLDIRKQILIYPSVDYTMASASYESNGAGFLLETPRVKWYFEQYFQDAALDTEKVRAAS
PLHGRIGSGLPETLIFTAGCDPLRDEGRAYAEALQCAGVKVEHHGLEDMIHAYMLLHDLVAEECLRTYEHIARFIKAV

Sequences:

>Translated_318_residues
MQDSPRGKASAKLAEFLTQANHNIALVREQNIQFTPALLRENLNKLAAFMTCKPEVNYIADKAWQLDGRIIAARVYSPAP
HSALPVVVHFHGGGHMCGSVALYDPICRQLASTAQCVVISVEYRLAPEYPYPAGLEDCEHALRHYAEVLEDVAFIAGPCI
MGDSAGGAICTSLSERSLADPTLDIRKQILIYPSVDYTMASASYESNGAGFLLETPRVKWYFEQYFQDAALDTEKVRAAS
PLHGRIGSGLPETLIFTAGCDPLRDEGRAYAEALQCAGVKVEHHGLEDMIHAYMLLHDLVAEECLRTYEHIARFIKAV
>Mature_318_residues
MQDSPRGKASAKLAEFLTQANHNIALVREQNIQFTPALLRENLNKLAAFMTCKPEVNYIADKAWQLDGRIIAARVYSPAP
HSALPVVVHFHGGGHMCGSVALYDPICRQLASTAQCVVISVEYRLAPEYPYPAGLEDCEHALRHYAEVLEDVAFIAGPCI
MGDSAGGAICTSLSERSLADPTLDIRKQILIYPSVDYTMASASYESNGAGFLLETPRVKWYFEQYFQDAALDTEKVRAAS
PLHGRIGSGLPETLIFTAGCDPLRDEGRAYAEALQCAGVKVEHHGLEDMIHAYMLLHDLVAEECLRTYEHIARFIKAV

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=287, Percent_Identity=29.616724738676, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI68051721, Length=301, Percent_Identity=27.906976744186, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI206597554, Length=286, Percent_Identity=28.3216783216783, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI226423947, Length=309, Percent_Identity=26.8608414239482, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI61966717, Length=315, Percent_Identity=24.1269841269841, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI157041239, Length=153, Percent_Identity=32.6797385620915, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI21328446, Length=98, Percent_Identity=34.6938775510204, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI1786682, Length=246, Percent_Identity=27.2357723577236, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI72001146, Length=331, Percent_Identity=26.5861027190332, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17567059, Length=269, Percent_Identity=30.4832713754647, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17540028, Length=170, Percent_Identity=32.9411764705882, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI71996133, Length=170, Percent_Identity=31.7647058823529, Blast_Score=88, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 35011; Mature: 35011

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDSPRGKASAKLAEFLTQANHNIALVREQNIQFTPALLRENLNKLAAFMTCKPEVNYIA
CCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHH
DKAWQLDGRIIAARVYSPAPHSALPVVVHFHGGGHMCGSVALYDPICRQLASTAQCVVIS
HHHEECCCEEEEEEEECCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEEEE
VEYRLAPEYPYPAGLEDCEHALRHYAEVLEDVAFIAGPCIMGDSAGGAICTSLSERSLAD
EEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHCCCCC
PTLDIRKQILIYPSVDYTMASASYESNGAGFLLETPRVKWYFEQYFQDAALDTEKVRAAS
CCHHCCCEEEEECCCCCEEECCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCHHHHHCCC
PLHGRIGSGLPETLIFTAGCDPLRDEGRAYAEALQCAGVKVEHHGLEDMIHAYMLLHDLV
CCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHH
AEECLRTYEHIARFIKAV
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQDSPRGKASAKLAEFLTQANHNIALVREQNIQFTPALLRENLNKLAAFMTCKPEVNYIA
CCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHH
DKAWQLDGRIIAARVYSPAPHSALPVVVHFHGGGHMCGSVALYDPICRQLASTAQCVVIS
HHHEECCCEEEEEEEECCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEEEE
VEYRLAPEYPYPAGLEDCEHALRHYAEVLEDVAFIAGPCIMGDSAGGAICTSLSERSLAD
EEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHCCCCC
PTLDIRKQILIYPSVDYTMASASYESNGAGFLLETPRVKWYFEQYFQDAALDTEKVRAAS
CCHHCCCEEEEECCCCCEEECCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCHHHHHCCC
PLHGRIGSGLPETLIFTAGCDPLRDEGRAYAEALQCAGVKVEHHGLEDMIHAYMLLHDLV
CCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHH
AEECLRTYEHIARFIKAV
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1907455 [H]