Click here to switch to the map view.

The map label for this gene is birA [H]

Identifier: 119773338

GI number: 119773338

Start: 230961

End: 231914

Strand: Direct

Name: birA [H]

Synonym: Sama_0197

Alternate gene names: 119773338

Gene position: 230961-231914 (Clockwise)

Preceding gene: 119773337

Following gene: 119773340

Centisome position: 5.36

GC content: 50.52

Gene sequence:

>954_bases
ATGGAACAGTGGCAACGTAAACGCGACATTATTGCGATGCTGTCAACGCAGGAGTTTGTGTCAGGCGAAGCCCTTGCCGA
ACGACTTGGTATTTCACGTGCTGCGATCAGTAAGCATATTGATGTGCTTGAAACCTTGGGGCTCAGTATTTACAGCGTTA
AAGGCCGTGGCTACAAACTGGCAACGCCGGTTTCCCTGATTGATGAATCCAGGCTCAAACAAGGTATCGAGCGCCGGTGT
TTTTACTTTGATGACATTCCCAGCACCAATGCCTTTATGTTATCCCACTCAGAAGAGCTGGAAAGCGGCGATGTGTGTAT
AGCAGAGCACCAGTCTGCTGGACGTGGCCGAAGAGGTCGCAAATGGGTGTCTCCCTATGGCAGCCATCTGTACTTTTCCC
TATTTTGGCGTTTAACCGATGGCATGAGTAAAGCTATGGGGTTGAGCCTTGTTGTTGGTTGCTCTCTTGCCAAAGTACTT
AAGGACCTCGGTGTTGCAGGTATTGGACTTAAGTGGCCCAATGACGTCTATCTGGATGGTCGCAAACTTGCGGGTATTCT
GGTGGAGATGAAAGGTCAGGCCGATAGCAATTGTGAGCTGATTATTGGCATAGGCGTGAATATGGCCATGCCGGATGCAC
AGGGTGAAGTCATCGATCAGCCCTGGGCCGACTTGCAGGGCCTTGCCATGCCGGGTAAAACCGAGTTTGCAGTACGCTTA
CAACGGCAACTTCAGGCTGACCTTGCCTTGTTCGAGCGGGAAGGACTCAAAGCTTTTTTAGAACGTTGGCAAGCATCAGA
TAGCTTTATTGGTAAGCCGGTTAATTTGATTATGGCAGATAAGGTGGAAACCGGTATTTGCCGGGGCATTGATGAGCAAG
GGGCCTTGCTGCTGGAGGTCGCCGGTGAGAGCAAAAGCTATATCGGTGGGGAAATCAGTTTGCGGCCAGCGTAA

Upstream 100 bases:

>100_bases
ATGAGGTGCATACGCGCTTTGGTGTGCAGCTTGAACCTGAGCCCAATGTGATGGGTCGATTATCACTCTTAACCGAATAA
CTCTAAACAGGGAACTGCAA

Downstream 100 bases:

>100_bases
CACCCATTGAGCCAGATAATGCCGACCAAGCCTATATCCGCTGGTCGGCATTTTTTATGGTTCATTTACGCAGCAGTACC
CGATTCATCAAATGATCGGC

Product: biotin--protein ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MEQWQRKRDIIAMLSTQEFVSGEALAERLGISRAAISKHIDVLETLGLSIYSVKGRGYKLATPVSLIDESRLKQGIERRC
FYFDDIPSTNAFMLSHSEELESGDVCIAEHQSAGRGRRGRKWVSPYGSHLYFSLFWRLTDGMSKAMGLSLVVGCSLAKVL
KDLGVAGIGLKWPNDVYLDGRKLAGILVEMKGQADSNCELIIGIGVNMAMPDAQGEVIDQPWADLQGLAMPGKTEFAVRL
QRQLQADLALFEREGLKAFLERWQASDSFIGKPVNLIMADKVETGICRGIDEQGALLLEVAGESKSYIGGEISLRPA

Sequences:

>Translated_317_residues
MEQWQRKRDIIAMLSTQEFVSGEALAERLGISRAAISKHIDVLETLGLSIYSVKGRGYKLATPVSLIDESRLKQGIERRC
FYFDDIPSTNAFMLSHSEELESGDVCIAEHQSAGRGRRGRKWVSPYGSHLYFSLFWRLTDGMSKAMGLSLVVGCSLAKVL
KDLGVAGIGLKWPNDVYLDGRKLAGILVEMKGQADSNCELIIGIGVNMAMPDAQGEVIDQPWADLQGLAMPGKTEFAVRL
QRQLQADLALFEREGLKAFLERWQASDSFIGKPVNLIMADKVETGICRGIDEQGALLLEVAGESKSYIGGEISLRPA
>Mature_317_residues
MEQWQRKRDIIAMLSTQEFVSGEALAERLGISRAAISKHIDVLETLGLSIYSVKGRGYKLATPVSLIDESRLKQGIERRC
FYFDDIPSTNAFMLSHSEELESGDVCIAEHQSAGRGRRGRKWVSPYGSHLYFSLFWRLTDGMSKAMGLSLVVGCSLAKVL
KDLGVAGIGLKWPNDVYLDGRKLAGILVEMKGQADSNCELIIGIGVNMAMPDAQGEVIDQPWADLQGLAMPGKTEFAVRL
QRQLQADLALFEREGLKAFLERWQASDSFIGKPVNLIMADKVETGICRGIDEQGALLLEVAGESKSYIGGEISLRPA

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=311, Percent_Identity=49.8392282958199, Blast_Score=298, Evalue=3e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004408
- InterPro:   IPR004409
- InterPro:   IPR003142
- InterPro:   IPR004143
- InterPro:   IPR013196
- InterPro:   IPR008988
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02237 BPL_C; PF03099 BPL_LipA_LipB; PF08279 HTH_11 [H]

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 34856; Mature: 34856

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQWQRKRDIIAMLSTQEFVSGEALAERLGISRAAISKHIDVLETLGLSIYSVKGRGYKL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEECCCCEEE
ATPVSLIDESRLKQGIERRCFYFDDIPSTNAFMLSHSEELESGDVCIAEHQSAGRGRRGR
ECCHHHHHHHHHHHHHHHHCEEECCCCCCCEEEEECCCCCCCCCEEEEECCCCCCCCCCC
KWVSPYGSHLYFSLFWRLTDGMSKAMGLSLVVGCSLAKVLKDLGVAGIGLKWPNDVYLDG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCEEECC
RKLAGILVEMKGQADSNCELIIGIGVNMAMPDAQGEVIDQPWADLQGLAMPGKTEFAVRL
HHEEEEEEEECCCCCCCCEEEEEECCCEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
QRQLQADLALFEREGLKAFLERWQASDSFIGKPVNLIMADKVETGICRGIDEQGALLLEV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHCCCCCCCCEEEEE
AGESKSYIGGEISLRPA
CCCCCCCCCCEEEECCC
>Mature Secondary Structure
MEQWQRKRDIIAMLSTQEFVSGEALAERLGISRAAISKHIDVLETLGLSIYSVKGRGYKL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEECCCCEEE
ATPVSLIDESRLKQGIERRCFYFDDIPSTNAFMLSHSEELESGDVCIAEHQSAGRGRRGR
ECCHHHHHHHHHHHHHHHHCEEECCCCCCCEEEEECCCCCCCCCEEEEECCCCCCCCCCC
KWVSPYGSHLYFSLFWRLTDGMSKAMGLSLVVGCSLAKVLKDLGVAGIGLKWPNDVYLDG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCEEECC
RKLAGILVEMKGQADSNCELIIGIGVNMAMPDAQGEVIDQPWADLQGLAMPGKTEFAVRL
HHEEEEEEEECCCCCCCCEEEEEECCCEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
QRQLQADLALFEREGLKAFLERWQASDSFIGKPVNLIMADKVETGICRGIDEQGALLLEV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHCCCCCCCCEEEEE
AGESKSYIGGEISLRPA
CCCCCCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3899863; 3536662; 8265357; 9278503; 1311302; 1328157; 1409631; 11353844 [H]