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The map label for this gene is murI [H]

Identifier: 119773335

GI number: 119773335

Start: 222531

End: 223331

Strand: Direct

Name: murI [H]

Synonym: Sama_0194

Alternate gene names: 119773335

Gene position: 222531-223331 (Clockwise)

Preceding gene: 119773333

Following gene: 119773337

Centisome position: 5.17

GC content: 50.94

Gene sequence:

>801_bases
GTGGAGTTTGGCTTGGCACGTCCCATTCTGGTTTTCGATTCAGGTATAGGTGGTTTGTCGGTGTTGTCTGAGATACGGCA
GCTATTGCCATCCAATGACTTCTATTACCTGTTTGATAATGCGCGCCTGCCATACGGTGAACTTACCGAGCAGGTGCTGG
TGGACGGCTGTGTTGAGCTTGTTGTGTCAGCAGCTACCAAAATCAACGCCGCTCTGGTGGTTATCGCCTGTAATACGGCC
AGTACGCTGGTGTTGCCTGTTTTAAGGAGCAAGTTGACGATACCCGTCGTAGGAGTCGTTCCTGCAATCAAACCTGCCGC
AAGTCATACTCTCTCTGGTCATATAGGCCTACTCGCTACTCCAGCCACGGTAAAGCGTTCTTACACCCATGAGCTGGTCA
CCAAATTTGCCAGTGATTGTCAGGTGCATATGTTTGGCAGTTCGGAACTGGTGATGATGGCAGAGCAAAAGATGGCTGGA
GTGCCTGTAGACATGGAGAAGTTAACCGGAATTCTGGCGCCCATCCAGGCAACGCAAGTGGATGTGTTGGTACTGGGATG
TACGCATTTTCCCATGTTGGCATACGAATTATCCCAGATATTGGGGCCCGGGATTAAACTGCTCGATTCGGGCTTTGCCA
TTGCCAGCAGGGTGCAGCATGTACTGGAGGGCTTGGGTGAGCATCAGCATCCTGCGCCAAATAGTATGAAGGCGTGGTAT
ACCACGCCTTCACTGACACTGGGCTTAATCCAAAGCCTGACAGACTATGGATTCAGCGAAATAGCGCCTTTTACCCATTG
A

Upstream 100 bases:

>100_bases
AAATGGCCGCAAATTTTACCCCAGCGAAACCGTATGTCCAGCTTTCTGGCTTTGCCCTTTATTTTAGGCTGTGGGAAAAT
TCACGGGTTTTCATTTTCAG

Downstream 100 bases:

>100_bases
ACCAAGACTCAGTCGTCAGACTTCTCAGACTCATTGTCCTGATTCTTGGCTTCTCTTACTTTTAATGTCCTTTCCTGGAA
GGTGTAGTCATTCAACTTGA

Product: glutamate racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MEFGLARPILVFDSGIGGLSVLSEIRQLLPSNDFYYLFDNARLPYGELTEQVLVDGCVELVVSAATKINAALVVIACNTA
STLVLPVLRSKLTIPVVGVVPAIKPAASHTLSGHIGLLATPATVKRSYTHELVTKFASDCQVHMFGSSELVMMAEQKMAG
VPVDMEKLTGILAPIQATQVDVLVLGCTHFPMLAYELSQILGPGIKLLDSGFAIASRVQHVLEGLGEHQHPAPNSMKAWY
TTPSLTLGLIQSLTDYGFSEIAPFTH

Sequences:

>Translated_266_residues
MEFGLARPILVFDSGIGGLSVLSEIRQLLPSNDFYYLFDNARLPYGELTEQVLVDGCVELVVSAATKINAALVVIACNTA
STLVLPVLRSKLTIPVVGVVPAIKPAASHTLSGHIGLLATPATVKRSYTHELVTKFASDCQVHMFGSSELVMMAEQKMAG
VPVDMEKLTGILAPIQATQVDVLVLGCTHFPMLAYELSQILGPGIKLLDSGFAIASRVQHVLEGLGEHQHPAPNSMKAWY
TTPSLTLGLIQSLTDYGFSEIAPFTH
>Mature_266_residues
MEFGLARPILVFDSGIGGLSVLSEIRQLLPSNDFYYLFDNARLPYGELTEQVLVDGCVELVVSAATKINAALVVIACNTA
STLVLPVLRSKLTIPVVGVVPAIKPAASHTLSGHIGLLATPATVKRSYTHELVTKFASDCQVHMFGSSELVMMAEQKMAG
VPVDMEKLTGILAPIQATQVDVLVLGCTHFPMLAYELSQILGPGIKLLDSGFAIASRVQHVLEGLGEHQHPAPNSMKAWY
TTPSLTLGLIQSLTDYGFSEIAPFTH

Specific function: Provides the (R)-glutamate required for cell wall biosynthesis [H]

COG id: COG0796

COG function: function code M; Glutamate racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aspartate/glutamate racemases family [H]

Homologues:

Organism=Escherichia coli, GI87082355, Length=235, Percent_Identity=52.3404255319149, Blast_Score=219, Evalue=1e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015942
- InterPro:   IPR001920
- InterPro:   IPR018187
- InterPro:   IPR004391 [H]

Pfam domain/function: PF01177 Asp_Glu_race [H]

EC number: =5.1.1.3 [H]

Molecular weight: Translated: 28483; Mature: 28483

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS00923 ASP_GLU_RACEMASE_1 ; PS00924 ASP_GLU_RACEMASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFGLARPILVFDSGIGGLSVLSEIRQLLPSNDFYYLFDNARLPYGELTEQVLVDGCVEL
CCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
VVSAATKINAALVVIACNTASTLVLPVLRSKLTIPVVGVVPAIKPAASHTLSGHIGLLAT
HHHHHHHCCEEEEEEEECCCHHHHHHHHHCCCCCCEEHHHCCCCCHHCCCCCCCCEEEEC
PATVKRSYTHELVTKFASDCQVHMFGSSELVMMAEQKMAGVPVDMEKLTGILAPIQATQV
CHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEHHHHCCCCCCHHHHHHHHCCCCCCEE
DVLVLGCTHFPMLAYELSQILGPGIKLLDSGFAIASRVQHVLEGLGEHQHPAPNSMKAWY
EEEEECCCCHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEE
TTPSLTLGLIQSLTDYGFSEIAPFTH
ECCHHHHHHHHHHHHCCHHHCCCCCC
>Mature Secondary Structure
MEFGLARPILVFDSGIGGLSVLSEIRQLLPSNDFYYLFDNARLPYGELTEQVLVDGCVEL
CCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
VVSAATKINAALVVIACNTASTLVLPVLRSKLTIPVVGVVPAIKPAASHTLSGHIGLLAT
HHHHHHHCCEEEEEEEECCCHHHHHHHHHCCCCCCEEHHHCCCCCHHCCCCCCCCEEEEC
PATVKRSYTHELVTKFASDCQVHMFGSSELVMMAEQKMAGVPVDMEKLTGILAPIQATQV
CHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEHHHHCCCCCCHHHHHHHHCCCCCCEE
DVLVLGCTHFPMLAYELSQILGPGIKLLDSGFAIASRVQHVLEGLGEHQHPAPNSMKAWY
EEEEECCCCHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEE
TTPSLTLGLIQSLTDYGFSEIAPFTH
ECCHHHHHHHHHHHHCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA