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The map label for this gene is selU

Identifier: 119773330

GI number: 119773330

Start: 217155

End: 218258

Strand: Reverse

Name: selU

Synonym: Sama_0189

Alternate gene names: 119773330

Gene position: 218258-217155 (Counterclockwise)

Preceding gene: 119773331

Following gene: 119773323

Centisome position: 5.07

GC content: 53.35

Gene sequence:

>1104_bases
ATGAGTACCAATACCGTCCCCGCCAAGCAATATCGGGAGATCTTCTTAAAAGGTCATCCCATTATGGATGTGCGCGCCCC
CATCGAGTTCAGCAAGGGCGCTTTTCCCAATGCGGTGAATCTGCCTCTGATGACCGACAGCGAACGGCAAAAGGTGGGAA
CCTGCTACAAAGAGCATGGCCAGGAAGCAGCTATAGAGCTTGGCCACCAGCTGGTTGCCGGTAAGGTCAAAGCGAACCGA
GTCGATGCCTGGAAAGCCTTTTTCAGCGAGCATCCAGATGGTTTTCTGTATTGCTTTCGTGGCGGGTTGAGATCGCGCAT
CACTCAAAGTTGGCTCAAAGAGGTCGGACTCGATATTCCCTTTATCGAAGGCGGCTACAAAGCAATGCGCCAATTCTTGA
TTGACGAGATTGACACTGCGCCAGGCAAAAGCGAAATGCTTATCCTCAGCGGCATTACCGGCAGCGGTAAAACTGACTTT
GTACGCCTTCGCAGTGAGTCGGTCGATCTGGAAGGCATCGCCAACCATCGGGGCTCCAGCTTTGGTCGCCAGCACGAGGG
GCAACCCAGTCAGATTGATTTTGAGAATCGGCTCGCCATCGAACTGCTGCGTCATCAGGAGCGCGAACAAGCCAAACTGC
TACTGGAAGATGAAAGCTTCCTCATTGGCCGCAATGCCATCCCCAAGACCTTTTTCGAAAAGATGCAGCAGGCGCCGGTA
TTGGTGCTGACTTCTACGCTCGAAGAACGTCTGCCTCGCCTGTTGGACGATTACGTCCACAAGATGCACGCGGGTTATGT
GGAACGTCTGGGTGAAGAGCAGGGCTTTATCGCTTATCGTGACTATCTGCTCGGCAGCCTCAAGGGCATCTACAAACGCC
TTGGCGGTAAGCTGCATGCTGAAATGACAGAGCTGATGAACAAAGCACTGGCCACGGAGCAAAGCCGCAGGGATACCAGT
GCCCACCTGGACTGGATATCACTGCTGCTGGAAAGTTATTACGACCCCATGTACCACTATCAACTGGGCAAAAAAGCCGC
CCGGGTGCGCTTTACCGGAACTCACGCTGAAATGCACGAGTATCTGAGTAGTCTCGGCAAATAG

Upstream 100 bases:

>100_bases
CCTTATGCGAGCTCCTCAGCAGCCACGGTATAACGCCTGTATGCATTGGCGAGCTGAAGGCGGGCCCGGGTAGACTGCTT
CTCAAATCCGGTGGTTTGGC

Downstream 100 bases:

>100_bases
CAAAAAGCCCTCGAAAGAGGGCTTTCATCTAAATGATGCCAAGTGGGCTCAGTTCAACCAGACCGTAATATCTTCATAGT
CGTAGTTATCAAAAAACGCG

Product: tRNA 2-selenouridine synthase

Products: NA

Alternate protein names: Selenophosphate-dependent tRNA 2-selenouridine synthase

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MSTNTVPAKQYREIFLKGHPIMDVRAPIEFSKGAFPNAVNLPLMTDSERQKVGTCYKEHGQEAAIELGHQLVAGKVKANR
VDAWKAFFSEHPDGFLYCFRGGLRSRITQSWLKEVGLDIPFIEGGYKAMRQFLIDEIDTAPGKSEMLILSGITGSGKTDF
VRLRSESVDLEGIANHRGSSFGRQHEGQPSQIDFENRLAIELLRHQEREQAKLLLEDESFLIGRNAIPKTFFEKMQQAPV
LVLTSTLEERLPRLLDDYVHKMHAGYVERLGEEQGFIAYRDYLLGSLKGIYKRLGGKLHAEMTELMNKALATEQSRRDTS
AHLDWISLLLESYYDPMYHYQLGKKAARVRFTGTHAEMHEYLSSLGK

Sequences:

>Translated_367_residues
MSTNTVPAKQYREIFLKGHPIMDVRAPIEFSKGAFPNAVNLPLMTDSERQKVGTCYKEHGQEAAIELGHQLVAGKVKANR
VDAWKAFFSEHPDGFLYCFRGGLRSRITQSWLKEVGLDIPFIEGGYKAMRQFLIDEIDTAPGKSEMLILSGITGSGKTDF
VRLRSESVDLEGIANHRGSSFGRQHEGQPSQIDFENRLAIELLRHQEREQAKLLLEDESFLIGRNAIPKTFFEKMQQAPV
LVLTSTLEERLPRLLDDYVHKMHAGYVERLGEEQGFIAYRDYLLGSLKGIYKRLGGKLHAEMTELMNKALATEQSRRDTS
AHLDWISLLLESYYDPMYHYQLGKKAARVRFTGTHAEMHEYLSSLGK
>Mature_366_residues
STNTVPAKQYREIFLKGHPIMDVRAPIEFSKGAFPNAVNLPLMTDSERQKVGTCYKEHGQEAAIELGHQLVAGKVKANRV
DAWKAFFSEHPDGFLYCFRGGLRSRITQSWLKEVGLDIPFIEGGYKAMRQFLIDEIDTAPGKSEMLILSGITGSGKTDFV
RLRSESVDLEGIANHRGSSFGRQHEGQPSQIDFENRLAIELLRHQEREQAKLLLEDESFLIGRNAIPKTFFEKMQQAPVL
VLTSTLEERLPRLLDDYVHKMHAGYVERLGEEQGFIAYRDYLLGSLKGIYKRLGGKLHAEMTELMNKALATEQSRRDTSA
HLDWISLLLESYYDPMYHYQLGKKAARVRFTGTHAEMHEYLSSLGK

Specific function: Catalyzes the transfer of selenium from selenophosphate for conversion of 2-thiouridine to 2-selenouridine at the wobble position in tRNA

COG id: COG2603

COG function: function code R; Predicted ATPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain

Homologues:

Organism=Escherichia coli, GI1786712, Length=357, Percent_Identity=46.4985994397759, Blast_Score=331, Evalue=4e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SELU_SHEAM (A1S1Z4)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_926070.1
- ProteinModelPortal:   A1S1Z4
- SMR:   A1S1Z4
- STRING:   A1S1Z4
- GeneID:   4602445
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_0189
- NMPDR:   fig|326297.7.peg.179
- eggNOG:   COG2603
- HOGENOM:   HBG452364
- OMA:   NLPLMND
- PhylomeDB:   A1S1Z4
- ProtClustDB:   PRK11784
- HAMAP:   MF_01622
- InterPro:   IPR001763
- InterPro:   IPR017582
- Gene3D:   G3DSA:3.40.250.10
- SMART:   SM00450
- TIGRFAMs:   TIGR03167

Pfam domain/function: PF00581 Rhodanese; SSF52821 Rhodanese-like

EC number: NA

Molecular weight: Translated: 41726; Mature: 41595

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS50206 RHODANESE_3

Important sites: ACT_SITE 98-98

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTNTVPAKQYREIFLKGHPIMDVRAPIEFSKGAFPNAVNLPLMTDSERQKVGTCYKEHG
CCCCCCCHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHC
QEAAIELGHQLVAGKVKANRVDAWKAFFSEHPDGFLYCFRGGLRSRITQSWLKEVGLDIP
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEHHCCHHHHHHHHHHHHHCCCCC
FIEGGYKAMRQFLIDEIDTAPGKSEMLILSGITGSGKTDFVRLRSESVDLEGIANHRGSS
CCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCEEEECCCCCCHHHHHHCCCCC
FGRQHEGQPSQIDFENRLAIELLRHQEREQAKLLLEDESFLIGRNAIPKTFFEKMQQAPV
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCEEECCCCCCHHHHHHHHHCCE
LVLTSTLEERLPRLLDDYVHKMHAGYVERLGEEQGFIAYRDYLLGSLKGIYKRLGGKLHA
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHH
EMTELMNKALATEQSRRDTSAHLDWISLLLESYYDPMYHYQLGKKAARVRFTGTHAEMHE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCHHHEEEEECCHHHHHH
YLSSLGK
HHHHHCC
>Mature Secondary Structure 
STNTVPAKQYREIFLKGHPIMDVRAPIEFSKGAFPNAVNLPLMTDSERQKVGTCYKEHG
CCCCCCHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHC
QEAAIELGHQLVAGKVKANRVDAWKAFFSEHPDGFLYCFRGGLRSRITQSWLKEVGLDIP
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEHHCCHHHHHHHHHHHHHCCCCC
FIEGGYKAMRQFLIDEIDTAPGKSEMLILSGITGSGKTDFVRLRSESVDLEGIANHRGSS
CCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCEEEECCCCCCHHHHHHCCCCC
FGRQHEGQPSQIDFENRLAIELLRHQEREQAKLLLEDESFLIGRNAIPKTFFEKMQQAPV
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCEEECCCCCCHHHHHHHHHCCE
LVLTSTLEERLPRLLDDYVHKMHAGYVERLGEEQGFIAYRDYLLGSLKGIYKRLGGKLHA
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHH
EMTELMNKALATEQSRRDTSAHLDWISLLLESYYDPMYHYQLGKKAARVRFTGTHAEMHE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCHHHEEEEECCHHHHHH
YLSSLGK
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA