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The map label for this gene is pckA

Identifier: 119773306

GI number: 119773306

Start: 193980

End: 195521

Strand: Reverse

Name: pckA

Synonym: Sama_0165

Alternate gene names: 119773306

Gene position: 195521-193980 (Counterclockwise)

Preceding gene: 119773307

Following gene: 119773305

Centisome position: 4.54

GC content: 57.39

Gene sequence:

>1542_bases
ATGGCGGAAGGAATTAACCGCGTACACCGTAATCCCTCAACGGCCGAGCTGGTAGAACTGGCGCTGAGAAGAGGTGAAGG
AGAATTAACCGCCAATGGCGCCCTGGTTGCCAAAACCGGAGCCCGTACAGGCCGTTCTCCCAATGACCGCTTCATCGTCA
AAGAAGCCGGTTCTGAAGCCGATATCGAATGGGGCAACGTCAACAAGCCCTTCGCTCCAGAGGCCTTCAATGCCCTGTGG
GATCGCGTAGCCGCTTACCTGGCCGACAAAGAAGTGTTCGTGTCCGATCTGGAAGTCGGTGCCGATCCAGAGCACTACCT
GCCAGTGACAGTGACCACCGAATATGCGTGGCATCAGCTGTTTGCCCGCAACCTGTTTATTATCCCTGAACACTTCAATC
AGGCCGGCAAGCCCACCTGGCAAATCATGAACGCCCCTGGCTTCGTGTGTGAGCCAGAACGCGATGGCACAGCCTCAGAA
GCCACAGTTATCATCAACTTTGCCGAGCGCAAAGTGCTGCTCGCCGGTCTCAAGTACGCCGGTGAGATGAAAAAATCCAT
GTTCTCCGTGCAGAACTTCCTGCTGCCTGCCAAGGGCGTGCTGCCAATGCACTGCTCCGCCAACGTAGGCAAAGACGGCG
ACACCACCCTGTTCTTCGGCCTGTCCGGTACCGGTAAGACCACCCTGTCCGCCGATCCCAAGCGTTTCCTCATCGGTGAC
GACGAGCACGGCTGGGCACCCGGCAGCGTGTTCAATATCGAAGGCGGCTGCTACGCCAAGTGTATCGACCTGAGCCAGAA
GAATGAGCCGGTAATTTGGGATGCCATCCGTTTCGGCACTGTGCTGGAAAACGTGGTACTGGACGACAAGCGCGTACCTG
ATTACAAAAACGCCAGCCTGACCGAAAACACCCGCGCCGCCTACCCACTGGAACACATTGCCCAGCGCCAGGAAGAAAAC
CGTGGCGGTGAGCCACGCGCCGTGGTGTTCCTGACCTGTGACGTATCCGGTGTGCTGCCTCCAGTGTCCAAGCTGACCAA
GGAACAGGCCGCATACCACTTCCTGTCTGGCTATACCGCCAAAGTAGGTTCTACTGAGATGGGCTCAACCGCCGCCATCC
AGTCAACCTTCTCTACCTGTTTCGGTGCCCCATTCTTCCCACGCCCAGCCGGTGTTTACGCCGAACTGCTGATGAAGCGT
ATCGAAGAATTCGGCAGCCAGGTTTATCTGGTGAACACCGGCTGGACCGGCGGTCCTTATGGCGTGGGTAAGCGTTTCGA
CATCCCAACCACCCGTGCCATCGTTGATGCCATCGTCAGCGGCGAACTGGCCAGTGTTGAAACCGTTCACCTGGAGAAGC
TGAATCTGGAAGTGCCTGTGGCCGTTCCAGGTGTTGAAACAGCCCTGCTGAATCCGGTAAACACCTGGGCCGACAAGGCC
AAGTACCAGGAATACGCGCAGAAGCTGGCTGAAGAGTTCCAGGCCAACTTTGCCAAGTATCAGGTACCTGACTCCATCAA
GAACGCCGGCCCCAAGGCCTGA

Upstream 100 bases:

>100_bases
GCCGCGGGCGCCCGACCCGATAAATCAAGGCGCACCACAAACCGGCAACACAGATCGGTACCCATAAAATAAAACCTCTT
GATATTTGGAGACCTTACCT

Downstream 100 bases:

>100_bases
TAGCATCTTGATGTAAAAAGCATTAAAACGGCTCCTGCGGGGGCCGTTTTTTATTGTCCGCAACAGGCAAATCCTGTGAT
TTACGCTCAAATACCCCGCA

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase

Number of amino acids: Translated: 513; Mature: 512

Protein sequence:

>513_residues
MAEGINRVHRNPSTAELVELALRRGEGELTANGALVAKTGARTGRSPNDRFIVKEAGSEADIEWGNVNKPFAPEAFNALW
DRVAAYLADKEVFVSDLEVGADPEHYLPVTVTTEYAWHQLFARNLFIIPEHFNQAGKPTWQIMNAPGFVCEPERDGTASE
ATVIINFAERKVLLAGLKYAGEMKKSMFSVQNFLLPAKGVLPMHCSANVGKDGDTTLFFGLSGTGKTTLSADPKRFLIGD
DEHGWAPGSVFNIEGGCYAKCIDLSQKNEPVIWDAIRFGTVLENVVLDDKRVPDYKNASLTENTRAAYPLEHIAQRQEEN
RGGEPRAVVFLTCDVSGVLPPVSKLTKEQAAYHFLSGYTAKVGSTEMGSTAAIQSTFSTCFGAPFFPRPAGVYAELLMKR
IEEFGSQVYLVNTGWTGGPYGVGKRFDIPTTRAIVDAIVSGELASVETVHLEKLNLEVPVAVPGVETALLNPVNTWADKA
KYQEYAQKLAEEFQANFAKYQVPDSIKNAGPKA

Sequences:

>Translated_513_residues
MAEGINRVHRNPSTAELVELALRRGEGELTANGALVAKTGARTGRSPNDRFIVKEAGSEADIEWGNVNKPFAPEAFNALW
DRVAAYLADKEVFVSDLEVGADPEHYLPVTVTTEYAWHQLFARNLFIIPEHFNQAGKPTWQIMNAPGFVCEPERDGTASE
ATVIINFAERKVLLAGLKYAGEMKKSMFSVQNFLLPAKGVLPMHCSANVGKDGDTTLFFGLSGTGKTTLSADPKRFLIGD
DEHGWAPGSVFNIEGGCYAKCIDLSQKNEPVIWDAIRFGTVLENVVLDDKRVPDYKNASLTENTRAAYPLEHIAQRQEEN
RGGEPRAVVFLTCDVSGVLPPVSKLTKEQAAYHFLSGYTAKVGSTEMGSTAAIQSTFSTCFGAPFFPRPAGVYAELLMKR
IEEFGSQVYLVNTGWTGGPYGVGKRFDIPTTRAIVDAIVSGELASVETVHLEKLNLEVPVAVPGVETALLNPVNTWADKA
KYQEYAQKLAEEFQANFAKYQVPDSIKNAGPKA
>Mature_512_residues
AEGINRVHRNPSTAELVELALRRGEGELTANGALVAKTGARTGRSPNDRFIVKEAGSEADIEWGNVNKPFAPEAFNALWD
RVAAYLADKEVFVSDLEVGADPEHYLPVTVTTEYAWHQLFARNLFIIPEHFNQAGKPTWQIMNAPGFVCEPERDGTASEA
TVIINFAERKVLLAGLKYAGEMKKSMFSVQNFLLPAKGVLPMHCSANVGKDGDTTLFFGLSGTGKTTLSADPKRFLIGDD
EHGWAPGSVFNIEGGCYAKCIDLSQKNEPVIWDAIRFGTVLENVVLDDKRVPDYKNASLTENTRAAYPLEHIAQRQEENR
GGEPRAVVFLTCDVSGVLPPVSKLTKEQAAYHFLSGYTAKVGSTEMGSTAAIQSTFSTCFGAPFFPRPAGVYAELLMKRI
EEFGSQVYLVNTGWTGGPYGVGKRFDIPTTRAIVDAIVSGELASVETVHLEKLNLEVPVAVPGVETALLNPVNTWADKAK
YQEYAQKLAEEFQANFAKYQVPDSIKNAGPKA

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family

Homologues:

Organism=Escherichia coli, GI1789807, Length=518, Percent_Identity=47.4903474903475, Blast_Score=433, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6322950, Length=524, Percent_Identity=45.0381679389313, Blast_Score=432, Evalue=1e-122,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): PCKA_SHEAM (A1S1X0)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_926046.1
- ProteinModelPortal:   A1S1X0
- SMR:   A1S1X0
- STRING:   A1S1X0
- GeneID:   4606033
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_0165
- NMPDR:   fig|326297.7.peg.156
- eggNOG:   COG1866
- HOGENOM:   HBG293387
- OMA:   FAQDLYG
- PhylomeDB:   A1S1X0
- ProtClustDB:   PRK09344
- GO:   GO:0005737
- GO:   GO:0006094
- HAMAP:   MF_00453
- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994
- Gene3D:   G3DSA:3.90.228.20
- Gene3D:   G3DSA:3.40.449.10
- PIRSF:   PIRSF006294
- TIGRFAMs:   TIGR00224

Pfam domain/function: PF01293 PEPCK_ATP; SSF68923 PEP_carboxykinase_N

EC number: =4.1.1.49

Molecular weight: Translated: 55975; Mature: 55844

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEGINRVHRNPSTAELVELALRRGEGELTANGALVAKTGARTGRSPNDRFIVKEAGSEA
CCCCHHHHHCCCCHHHHHHHHHHCCCCCEEECCEEEEECCCCCCCCCCCEEEEEECCCCC
DIEWGNVNKPFAPEAFNALWDRVAAYLADKEVFVSDLEVGADPEHYLPVTVTTEYAWHQL
CCEECCCCCCCCCHHHHHHHHHHHHHHHCCHHHEEHHHCCCCCCCEEEEEEECHHHHHHH
FARNLFIIPEHFNQAGKPTWQIMNAPGFVCEPERDGTASEATVIINFAERKVLLAGLKYA
HHCCEEEEEHHHCCCCCCEEEEECCCCCEECCCCCCCCCCEEEEEEECCCEEHHHHHHHH
GEMKKSMFSVQNFLLPAKGVLPMHCSANVGKDGDTTLFFGLSGTGKTTLSADPKRFLIGD
HHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEEECCCCCEEECCCCCEEEECC
DEHGWAPGSVFNIEGGCYAKCIDLSQKNEPVIWDAIRFGTVLENVVLDDKRVPDYKNASL
CCCCCCCCCEEEECCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCCCC
TENTRAAYPLEHIAQRQEENRGGEPRAVVFLTCDVSGVLPPVSKLTKEQAAYHFLSGYTA
CCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCEE
KVGSTEMGSTAAIQSTFSTCFGAPFFPRPAGVYAELLMKRIEEFGSQVYLVNTGWTGGPY
ECCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCC
GVGKRFDIPTTRAIVDAIVSGELASVETVHLEKLNLEVPVAVPGVETALLNPVNTWADKA
CCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEEECEEEEEECCCCHHHHCCCHHHHHHHH
KYQEYAQKLAEEFQANFAKYQVPDSIKNAGPKA
HHHHHHHHHHHHHHHHHHEEECCHHHHCCCCCC
>Mature Secondary Structure 
AEGINRVHRNPSTAELVELALRRGEGELTANGALVAKTGARTGRSPNDRFIVKEAGSEA
CCCHHHHHCCCCHHHHHHHHHHCCCCCEEECCEEEEECCCCCCCCCCCEEEEEECCCCC
DIEWGNVNKPFAPEAFNALWDRVAAYLADKEVFVSDLEVGADPEHYLPVTVTTEYAWHQL
CCEECCCCCCCCCHHHHHHHHHHHHHHHCCHHHEEHHHCCCCCCCEEEEEEECHHHHHHH
FARNLFIIPEHFNQAGKPTWQIMNAPGFVCEPERDGTASEATVIINFAERKVLLAGLKYA
HHCCEEEEEHHHCCCCCCEEEEECCCCCEECCCCCCCCCCEEEEEEECCCEEHHHHHHHH
GEMKKSMFSVQNFLLPAKGVLPMHCSANVGKDGDTTLFFGLSGTGKTTLSADPKRFLIGD
HHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEEECCCCCEEECCCCCEEEECC
DEHGWAPGSVFNIEGGCYAKCIDLSQKNEPVIWDAIRFGTVLENVVLDDKRVPDYKNASL
CCCCCCCCCEEEECCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCCCC
TENTRAAYPLEHIAQRQEENRGGEPRAVVFLTCDVSGVLPPVSKLTKEQAAYHFLSGYTA
CCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCEE
KVGSTEMGSTAAIQSTFSTCFGAPFFPRPAGVYAELLMKRIEEFGSQVYLVNTGWTGGPY
ECCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCC
GVGKRFDIPTTRAIVDAIVSGELASVETVHLEKLNLEVPVAVPGVETALLNPVNTWADKA
CCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEEECEEEEEECCCCHHHHCCCHHHHHHHH
KYQEYAQKLAEEFQANFAKYQVPDSIKNAGPKA
HHHHHHHHHHHHHHHHHHEEECCHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA