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The map label for this gene is btuB [H]

Identifier: 119773273

GI number: 119773273

Start: 146791

End: 148878

Strand: Direct

Name: btuB [H]

Synonym: Sama_0131

Alternate gene names: 119773273

Gene position: 146791-148878 (Clockwise)

Preceding gene: 119773271

Following gene: 119773274

Centisome position: 3.41

GC content: 58.96

Gene sequence:

>2088_bases
ATGACACTCAAACCACCCGCTTTCGCACTATCGCTCTTGTCTCTTTCCCTGCTTCCCTTGCTGGGGCATGCCAGCGAGAC
CCGTGATCCTCTGCTCAATATTGCCGATGTGATTGTGGTCCGCGGTGAAAAGCCCACCGCCGAAGCGCTGGCAACCACCC
ATTGGCGTATTGATGCCGAGGATATTCGCAACAGCGGTGCCCAAACGCTGGATCAGGTGCTGGCTACGGTGCCCGGCATC
CATGTGCGTACCGGTGGGGAGGGTACGCCCCGAATTGATATCCGTGGTCTTAAAACCCGTCACGTTACCCTGCTGATTAA
CGGGGTGCCGGCCGCCAGTGCCGCAGATGGCCAGTTCGATCCCAGTGTGATCCCAACCAGCCAGATTGCCGCTGTCGATA
TCGCCGTGGGTCCTGCGTCCGTGCTCTATGGCCCGGGTGGTGCAGGTGGCGTAATCAACGTGATTACCCGCAGTGGTGAC
GAGGCTTCGGTATCCGGGAAGCTTGAGTTTGCCGGTTCGGATACCCTCAACGCTGACGTGGCATTGGGTGGCGGTGGCGA
CAACTGGCAGGGCTTTGTCAGCGCGGGTAAGCAAAAGCGCGAAAGCTGGCCGTTGTCGGATGATTACCAGGAAGCGCCCA
ATCAACCCGCAGGTGATCGCCTCAACGCCGACAGGGAAGTAGACAATTTTTATGGCCAGGGCAGCTACTGGCTGTCAGAT
GCCACTCAACTGACTGCCAACATTAATTTGCGCGAGGGCGACTGGGGTAAGCCGGGCCGTGACGGCAGCGGCTCAGGACG
TGCCAAGTTTGAACGTGTGGATGACTACCGGAATATCAGCAGTCAATTGGGCCTGGCCCATCGCTTTGGCAACGGCATGA
CGCTCAGGGGCTATGGCTACTACAACCGTACCGAAACCCTGGAGTCTGCCTACACCGACATCAGTTTCAGTGACCTCGAC
AGCCAGCAGCGCGGCGAGTCCACGGTGCAGGGGCTGAATCTACAACTTGTCACAGAGCTTGGCCGTCACAGTCTCCTTAC
TCTGGGTGCTGGTGCCGAAGAACAGCGCTGGGATGAGCTGGCGACTCAATATTCGACCTCTGACTCAGGCTCGGGCAGTG
GTTCAGGCGGTGGTTCAGGCGGTGGCTCTGGCAGTGGTACAGGCGGTGGTTCAGGCGGTGGCTCGGGCGGTGGTTCGGGC
AGTGGCTCGGGCGGAGGTTCAGGCAGTGGCTCCGGCGGCGGCTCCAGCAGCGGTGTACCCACAGAGACAGGCTTTGACGA
CAGTGCCTGGGTGTACAGTCTTGCGGCCGAGTATCAATATCAACGGGATAACTGGGGTGCAACCCTTGGCGGTGCCTGGT
ATCAGCGTGATGGCGATACCTCGGTGGATGAGCCTTCGGCCATGGCCGCACTGTACTGGCAGGCGCTGTCTCAGACACGT
CTGAGCGCTTCGGCGGCCCGCAAGGTGCGCTTCCCCTCCATGGAAAACCTGTATTCACTTTCTGCAGGCAATGGCGAACT
GACCGCTGAGACTTCAGAGCATTGGGAGTTAGGTCTGGAGCAGGGCTTCGCCCTCGGCAGTTTCGAGGTTTATGGTTACG
AGACCGGCGCCGATGATTACATCGCCAAGGGCACAGATGGTATCTACGCCAATCTGGGTGACTATCGATTCCGCGGTGTG
GATGCGCGGCTGTTGATATCACCTTTGCAAGCGCTGGATGTCTCTCTGAGCTACAGCTTCCTCGACAGTGAAGATAAAAC
ACCGGACAGCACCGAAGCGCTGCAATATCGCCCGCGCCATCAGTACGGCCTGACCCTGGATTATCGCTTCGACACCGATA
CCCGGGTGCACCTGTCCTGGCAGAAGGTGATGGATCAGATTTACTTTGAGCGCAGCGGCAAAACCATGGTCGAGCGTAAC
CTGCCGGACTACGACCTGCTTGACCTCAAGCTGTCGCAGTCGTTTTACGATGACAAGCTGGCGCTCTACATCAGGGCCAC
TAACCTGCTGGACGAAAACTACGCCGAGAGCGAGTCTCTGCCCCAGGCGGGTCGCCAGTGGTTTATCGGTATGGATTGGC
GCTTGTGA

Upstream 100 bases:

>100_bases
CAATAGGTCGATCGGGGGCAGATTTTGTCATTCTTTTGCTTGAGCCTGATTCGGCAGCTCTTTGAAATGACGCTATTCCT
ACGCCAAACGAAGAATCAGA

Downstream 100 bases:

>100_bases
CACAGCCGCCGCTCGTCAGCCTCGAGGACCTCGGCTACGGCCATGGTCCCGGGCAACGGCTGTTTGATGGCCTGTCGCTG
CATATTCATTCGGGCGAGTG

Product: TonB-dependent receptor

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 695; Mature: 694

Protein sequence:

>695_residues
MTLKPPAFALSLLSLSLLPLLGHASETRDPLLNIADVIVVRGEKPTAEALATTHWRIDAEDIRNSGAQTLDQVLATVPGI
HVRTGGEGTPRIDIRGLKTRHVTLLINGVPAASAADGQFDPSVIPTSQIAAVDIAVGPASVLYGPGGAGGVINVITRSGD
EASVSGKLEFAGSDTLNADVALGGGGDNWQGFVSAGKQKRESWPLSDDYQEAPNQPAGDRLNADREVDNFYGQGSYWLSD
ATQLTANINLREGDWGKPGRDGSGSGRAKFERVDDYRNISSQLGLAHRFGNGMTLRGYGYYNRTETLESAYTDISFSDLD
SQQRGESTVQGLNLQLVTELGRHSLLTLGAGAEEQRWDELATQYSTSDSGSGSGSGGGSGGGSGSGTGGGSGGGSGGGSG
SGSGGGSGSGSGGGSSSGVPTETGFDDSAWVYSLAAEYQYQRDNWGATLGGAWYQRDGDTSVDEPSAMAALYWQALSQTR
LSASAARKVRFPSMENLYSLSAGNGELTAETSEHWELGLEQGFALGSFEVYGYETGADDYIAKGTDGIYANLGDYRFRGV
DARLLISPLQALDVSLSYSFLDSEDKTPDSTEALQYRPRHQYGLTLDYRFDTDTRVHLSWQKVMDQIYFERSGKTMVERN
LPDYDLLDLKLSQSFYDDKLALYIRATNLLDENYAESESLPQAGRQWFIGMDWRL

Sequences:

>Translated_695_residues
MTLKPPAFALSLLSLSLLPLLGHASETRDPLLNIADVIVVRGEKPTAEALATTHWRIDAEDIRNSGAQTLDQVLATVPGI
HVRTGGEGTPRIDIRGLKTRHVTLLINGVPAASAADGQFDPSVIPTSQIAAVDIAVGPASVLYGPGGAGGVINVITRSGD
EASVSGKLEFAGSDTLNADVALGGGGDNWQGFVSAGKQKRESWPLSDDYQEAPNQPAGDRLNADREVDNFYGQGSYWLSD
ATQLTANINLREGDWGKPGRDGSGSGRAKFERVDDYRNISSQLGLAHRFGNGMTLRGYGYYNRTETLESAYTDISFSDLD
SQQRGESTVQGLNLQLVTELGRHSLLTLGAGAEEQRWDELATQYSTSDSGSGSGSGGGSGGGSGSGTGGGSGGGSGGGSG
SGSGGGSGSGSGGGSSSGVPTETGFDDSAWVYSLAAEYQYQRDNWGATLGGAWYQRDGDTSVDEPSAMAALYWQALSQTR
LSASAARKVRFPSMENLYSLSAGNGELTAETSEHWELGLEQGFALGSFEVYGYETGADDYIAKGTDGIYANLGDYRFRGV
DARLLISPLQALDVSLSYSFLDSEDKTPDSTEALQYRPRHQYGLTLDYRFDTDTRVHLSWQKVMDQIYFERSGKTMVERN
LPDYDLLDLKLSQSFYDDKLALYIRATNLLDENYAESESLPQAGRQWFIGMDWRL
>Mature_694_residues
TLKPPAFALSLLSLSLLPLLGHASETRDPLLNIADVIVVRGEKPTAEALATTHWRIDAEDIRNSGAQTLDQVLATVPGIH
VRTGGEGTPRIDIRGLKTRHVTLLINGVPAASAADGQFDPSVIPTSQIAAVDIAVGPASVLYGPGGAGGVINVITRSGDE
ASVSGKLEFAGSDTLNADVALGGGGDNWQGFVSAGKQKRESWPLSDDYQEAPNQPAGDRLNADREVDNFYGQGSYWLSDA
TQLTANINLREGDWGKPGRDGSGSGRAKFERVDDYRNISSQLGLAHRFGNGMTLRGYGYYNRTETLESAYTDISFSDLDS
QQRGESTVQGLNLQLVTELGRHSLLTLGAGAEEQRWDELATQYSTSDSGSGSGSGGGSGGGSGSGTGGGSGGGSGGGSGS
GSGGGSGSGSGGGSSSGVPTETGFDDSAWVYSLAAEYQYQRDNWGATLGGAWYQRDGDTSVDEPSAMAALYWQALSQTRL
SASAARKVRFPSMENLYSLSAGNGELTAETSEHWELGLEQGFALGSFEVYGYETGADDYIAKGTDGIYANLGDYRFRGVD
ARLLISPLQALDVSLSYSFLDSEDKTPDSTEALQYRPRHQYGLTLDYRFDTDTRVHLSWQKVMDQIYFERSGKTMVERNL
PDYDLLDLKLSQSFYDDKLALYIRATNLLDENYAESESLPQAGRQWFIGMDWRL

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG1629

COG function: function code P; Outer membrane receptor proteins, mostly Fe transport

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786798, Length=169, Percent_Identity=32.5443786982249, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI1788478, Length=105, Percent_Identity=34.2857142857143, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1790743, Length=157, Percent_Identity=34.3949044585987, Blast_Score=66, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010916
- InterPro:   IPR010917 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 74209; Mature: 74078

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLKPPAFALSLLSLSLLPLLGHASETRDPLLNIADVIVVRGEKPTAEALATTHWRIDAE
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHEEEEEEEECCCCCCHHHHHHEEEEEEHH
DIRNSGAQTLDQVLATVPGIHVRTGGEGTPRIDIRGLKTRHVTLLINGVPAASAADGQFD
HHHCCCHHHHHHHHHHCCCEEEEECCCCCCEEEEEECCEEEEEEEEECCCCCCCCCCCCC
PSVIPTSQIAAVDIAVGPASVLYGPGGAGGVINVITRSGDEASVSGKLEFAGSDTLNADV
CCCCCCCCEEEEEEEECCHHEEECCCCCCCEEEEEECCCCCCCCCEEEEECCCCCCCEEE
ALGGGGDNWQGFVSAGKQKRESWPLSDDYQEAPNQPAGDRLNADREVDNFYGQGSYWLSD
EECCCCCCCHHHHHHCHHHHHCCCCCCCHHHCCCCCCCCCCCCCCCHHHHCCCCCEEECC
ATQLTANINLREGDWGKPGRDGSGSGRAKFERVDDYRNISSQLGLAHRFGNGMTLRGYGY
CEEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHCCCCCEEEEECC
YNRTETLESAYTDISFSDLDSQQRGESTVQGLNLQLVTELGRHSLLTLGAGAEEQRWDEL
CCHHHHHHHHHCCCCHHHCCCHHCCCHHHHCCCCHHHHHCCCCEEEEECCCCCHHHHHHH
ATQYSTSDSGSGSGSGGGSGGGSGSGTGGGSGGGSGGGSGSGSGGGSGSGSGGGSSSGVP
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TETGFDDSAWVYSLAAEYQYQRDNWGATLGGAWYQRDGDTSVDEPSAMAALYWQALSQTR
CCCCCCCCHHHEEEHHHHEEECCCCCCEECCCEEECCCCCCCCCCHHHHHHHHHHHHHHH
LSASAARKVRFPSMENLYSLSAGNGELTAETSEHWELGLEQGFALGSFEVYGYETGADDY
HHHHHHHHCCCCCHHHHEEEECCCCCEEECCCHHHHHHHHCCCEECCEEEEEEECCCCCC
IAKGTDGIYANLGDYRFRGVDARLLISPLQALDVSLSYSFLDSEDKTPDSTEALQYRPRH
EECCCCCEEECCCCEEECCCCHHHHHHHHHHHCEEEEEEEECCCCCCCCCHHHHHCCCCC
QYGLTLDYRFDTDTRVHLSWQKVMDQIYFERSGKTMVERNLPDYDLLDLKLSQSFYDDKL
CCCCEEEEEECCCCEEEEEHHHHHHHHHHHCCCCCHHHCCCCCCCEEEEEECCCCCCCEE
ALYIRATNLLDENYAESESLPQAGRQWFIGMDWRL
EEEEEEEHHHCCCCHHHCCCCHHCCEEEEEECCCC
>Mature Secondary Structure 
TLKPPAFALSLLSLSLLPLLGHASETRDPLLNIADVIVVRGEKPTAEALATTHWRIDAE
CCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHEEEEEEEECCCCCCHHHHHHEEEEEEHH
DIRNSGAQTLDQVLATVPGIHVRTGGEGTPRIDIRGLKTRHVTLLINGVPAASAADGQFD
HHHCCCHHHHHHHHHHCCCEEEEECCCCCCEEEEEECCEEEEEEEEECCCCCCCCCCCCC
PSVIPTSQIAAVDIAVGPASVLYGPGGAGGVINVITRSGDEASVSGKLEFAGSDTLNADV
CCCCCCCCEEEEEEEECCHHEEECCCCCCCEEEEEECCCCCCCCCEEEEECCCCCCCEEE
ALGGGGDNWQGFVSAGKQKRESWPLSDDYQEAPNQPAGDRLNADREVDNFYGQGSYWLSD
EECCCCCCCHHHHHHCHHHHHCCCCCCCHHHCCCCCCCCCCCCCCCHHHHCCCCCEEECC
ATQLTANINLREGDWGKPGRDGSGSGRAKFERVDDYRNISSQLGLAHRFGNGMTLRGYGY
CEEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHCCCCCEEEEECC
YNRTETLESAYTDISFSDLDSQQRGESTVQGLNLQLVTELGRHSLLTLGAGAEEQRWDEL
CCHHHHHHHHHCCCCHHHCCCHHCCCHHHHCCCCHHHHHCCCCEEEEECCCCCHHHHHHH
ATQYSTSDSGSGSGSGGGSGGGSGSGTGGGSGGGSGGGSGSGSGGGSGSGSGGGSSSGVP
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TETGFDDSAWVYSLAAEYQYQRDNWGATLGGAWYQRDGDTSVDEPSAMAALYWQALSQTR
CCCCCCCCHHHEEEHHHHEEECCCCCCEECCCEEECCCCCCCCCCHHHHHHHHHHHHHHH
LSASAARKVRFPSMENLYSLSAGNGELTAETSEHWELGLEQGFALGSFEVYGYETGADDY
HHHHHHHHCCCCCHHHHEEEECCCCCEEECCCHHHHHHHHCCCEECCEEEEEEECCCCCC
IAKGTDGIYANLGDYRFRGVDARLLISPLQALDVSLSYSFLDSEDKTPDSTEALQYRPRH
EECCCCCEEECCCCEEECCCCHHHHHHHHHHHCEEEEEEEECCCCCCCCCHHHHHCCCCC
QYGLTLDYRFDTDTRVHLSWQKVMDQIYFERSGKTMVERNLPDYDLLDLKLSQSFYDDKL
CCCCEEEEEECCCCEEEEEHHHHHHHHHHHCCCCCHHHCCCCCCCEEEEEECCCCCCCEE
ALYIRATNLLDENYAESESLPQAGRQWFIGMDWRL
EEEEEEEHHHCCCCHHHCCCCHHCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA