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The map label for this gene is purB [H]

Identifier: 119718560

GI number: 119718560

Start: 4588817

End: 4590247

Strand: Reverse

Name: purB [H]

Synonym: Noca_4341

Alternate gene names: 119718560

Gene position: 4590247-4588817 (Counterclockwise)

Preceding gene: 119718561

Following gene: 119718558

Centisome position: 92.07

GC content: 72.47

Gene sequence:

>1431_bases
GTGACCGTCCCCAACGTCCTGGCCACCCGCTACGCCTCCGCCGAGCTGGCGCGGATCTGGTCGCCGGAGCACAAGATCGT
GCTGGAGCGGCAGCTCTGGATCGCGGTCCTGGAGGCGCAGCGCGACCTCGGGATCGCGGTGCCGGACGGTGTCGTCGAGG
CCTACCGCGGCGTCGCGGAGCTCGGTGAGAAGGGGGTGGACCTCGAGTCGATCGCGGCCCGCGAGCGGGTGACCCGGCAC
GACGTGAAGGCCCGGATCGAGGAGTTCTCGGCGCTGGCGGGTCACGAGCACATCCACAAGGGCATGACCTCGCGCGACCT
CACCGAGAACGTCGAGCAGCTGCAGGTCAGGCAGAGCGTGGCGCTGGTCCGCGACCGGGCGGTCGCGGCACTGGCCCGGC
TCGCCCGGCTGGCCGCCGAGCACGAGGCCACCGTGATGGCCGGACGCAGCCACAACGTCGCCGCCCAGGCGACCACGCTC
GGGAAGCGGTTCGCGACCGTGGCCGACGAGATGCTGGTCGCCCTCGACCGGGTCGAGGACCTGCTGGTGCGCTACCCGCT
GCGCGGGATCAAGGGGCCGATGGGGACCGCGCAGGACATGCTCGACCTGCTCGACGGCGACGCCGCCAAGCTGGCCGAGC
TCGAGCAGCGGGTCGCGGCGCACCTGGGCTTCGAGCGGGTGCTGACCAGCGTCGGGCAGGTCTACCCGCGCAGCCTCGAC
CTCGACGTCGTCAGCGCCCTGGTCCAGCTGGTGTCCGGACCGTCGAACCTCGCGACCACGATCCGGCTGATGGCCGGCAA
CGAGCTGGTGACCGAGGGCTTCAAGGAGGGCCAGGTCGGCTCCTCGGCGATGCCGCACAAGATGAACAGCCGCTCCGCCG
AGCGGGTCAACGGGCTCTCGGTCGTGCTCCGCGGCCACCTCTCGATGGTCAGCGAGCTGGCCGGCGACCAGTGGAACGAG
GGCGACGTCTCCGACTCCGTCGTCCGCCGGGTGGCCCTGCCGGACGCGTTCTTCGCCGCCGACGGGCTGTTCGAGACGTT
CCTGACGATCCTCGACGAGTTCGGGGCCTTCCCGGCCGTCATCCAGCGCGAGCTGGACCGCTACCTGCCGTTCCTCGCCA
CCACGAAGGTGCTGATGGCCGCGGTTCGCAACGGGGTGGGCCGGGAGGCCGCCCACGAGTCGATCAAGGAGGCGGCGGTC
GGCACCGCCCTCGACATGCGCCGCGGCCAGGCCGACAACGACGTCTTCGCCCGGCTGGCCGCCGACACCCGGCTGGGGCT
GACCTCCGACCAGCTGGCGTCCCTGGTGGCCGAGCCGATCACGTTCACCGGCGCGGCGGTCGCGCAGGTGCAGGCCGTCG
TACGCCGGGTCGCCGAGGTGACCGACCGGCACCCCGCGGCCGCGGCGTACGCACCCGGCGACATCCTCTAG

Upstream 100 bases:

>100_bases
GCCCGTTTACATCTTGTACCGACCCCGCTGCAACCTGGTGGGAACAGCAGGTAACCCGGCGTTACAGCCGGCGGCCGGGC
CCGTGGGAGAATGACCGCTC

Downstream 100 bases:

>100_bases
CCGGACCGGTCAGCCTGCGTCCTGCTGGCGCAGGGCGCGCACCGCCATGGCCAGCGCGGCGCACAGCACCACCACGCTCG
TGAGCAGCGCGGCGCGGAGC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MTVPNVLATRYASAELARIWSPEHKIVLERQLWIAVLEAQRDLGIAVPDGVVEAYRGVAELGEKGVDLESIAARERVTRH
DVKARIEEFSALAGHEHIHKGMTSRDLTENVEQLQVRQSVALVRDRAVAALARLARLAAEHEATVMAGRSHNVAAQATTL
GKRFATVADEMLVALDRVEDLLVRYPLRGIKGPMGTAQDMLDLLDGDAAKLAELEQRVAAHLGFERVLTSVGQVYPRSLD
LDVVSALVQLVSGPSNLATTIRLMAGNELVTEGFKEGQVGSSAMPHKMNSRSAERVNGLSVVLRGHLSMVSELAGDQWNE
GDVSDSVVRRVALPDAFFAADGLFETFLTILDEFGAFPAVIQRELDRYLPFLATTKVLMAAVRNGVGREAAHESIKEAAV
GTALDMRRGQADNDVFARLAADTRLGLTSDQLASLVAEPITFTGAAVAQVQAVVRRVAEVTDRHPAAAAYAPGDIL

Sequences:

>Translated_476_residues
MTVPNVLATRYASAELARIWSPEHKIVLERQLWIAVLEAQRDLGIAVPDGVVEAYRGVAELGEKGVDLESIAARERVTRH
DVKARIEEFSALAGHEHIHKGMTSRDLTENVEQLQVRQSVALVRDRAVAALARLARLAAEHEATVMAGRSHNVAAQATTL
GKRFATVADEMLVALDRVEDLLVRYPLRGIKGPMGTAQDMLDLLDGDAAKLAELEQRVAAHLGFERVLTSVGQVYPRSLD
LDVVSALVQLVSGPSNLATTIRLMAGNELVTEGFKEGQVGSSAMPHKMNSRSAERVNGLSVVLRGHLSMVSELAGDQWNE
GDVSDSVVRRVALPDAFFAADGLFETFLTILDEFGAFPAVIQRELDRYLPFLATTKVLMAAVRNGVGREAAHESIKEAAV
GTALDMRRGQADNDVFARLAADTRLGLTSDQLASLVAEPITFTGAAVAQVQAVVRRVAEVTDRHPAAAAYAPGDIL
>Mature_475_residues
TVPNVLATRYASAELARIWSPEHKIVLERQLWIAVLEAQRDLGIAVPDGVVEAYRGVAELGEKGVDLESIAARERVTRHD
VKARIEEFSALAGHEHIHKGMTSRDLTENVEQLQVRQSVALVRDRAVAALARLARLAAEHEATVMAGRSHNVAAQATTLG
KRFATVADEMLVALDRVEDLLVRYPLRGIKGPMGTAQDMLDLLDGDAAKLAELEQRVAAHLGFERVLTSVGQVYPRSLDL
DVVSALVQLVSGPSNLATTIRLMAGNELVTEGFKEGQVGSSAMPHKMNSRSAERVNGLSVVLRGHLSMVSELAGDQWNEG
DVSDSVVRRVALPDAFFAADGLFETFLTILDEFGAFPAVIQRELDRYLPFLATTKVLMAAVRNGVGREAAHESIKEAAVG
TALDMRRGQADNDVFARLAADTRLGLTSDQLASLVAEPITFTGAAVAQVQAVVRRVAEVTDRHPAAAAYAPGDIL

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=447, Percent_Identity=32.4384787472036, Blast_Score=238, Evalue=8e-63,
Organism=Homo sapiens, GI183227688, Length=385, Percent_Identity=32.4675324675325, Blast_Score=211, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI17508577, Length=455, Percent_Identity=30.5494505494506, Blast_Score=192, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI32564234, Length=347, Percent_Identity=31.4121037463977, Blast_Score=155, Evalue=5e-38,
Organism=Saccharomyces cerevisiae, GI6323391, Length=458, Percent_Identity=34.2794759825328, Blast_Score=246, Evalue=8e-66,
Organism=Drosophila melanogaster, GI24647570, Length=471, Percent_Identity=32.484076433121, Blast_Score=221, Evalue=7e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 51348; Mature: 51217

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVPNVLATRYASAELARIWSPEHKIVLERQLWIAVLEAQRDLGIAVPDGVVEAYRGVAE
CCCCHHHHHHHHHHHHHHHCCCCHHEEHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
LGEKGVDLESIAARERVTRHDVKARIEEFSALAGHEHIHKGMTSRDLTENVEQLQVRQSV
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
ALVRDRAVAALARLARLAAEHEATVMAGRSHNVAAQATTLGKRFATVADEMLVALDRVED
HHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLVRYPLRGIKGPMGTAQDMLDLLDGDAAKLAELEQRVAAHLGFERVLTSVGQVYPRSLD
HHHHHCHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCC
LDVVSALVQLVSGPSNLATTIRLMAGNELVTEGFKEGQVGSSAMPHKMNSRSAERVNGLS
HHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
VVLRGHLSMVSELAGDQWNEGDVSDSVVRRVALPDAFFAADGLFETFLTILDEFGAFPAV
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHH
IQRELDRYLPFLATTKVLMAAVRNGVGREAAHESIKEAAVGTALDMRRGQADNDVFARLA
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
ADTRLGLTSDQLASLVAEPITFTGAAVAQVQAVVRRVAEVTDRHPAAAAYAPGDIL
HHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TVPNVLATRYASAELARIWSPEHKIVLERQLWIAVLEAQRDLGIAVPDGVVEAYRGVAE
CCCHHHHHHHHHHHHHHHCCCCHHEEHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
LGEKGVDLESIAARERVTRHDVKARIEEFSALAGHEHIHKGMTSRDLTENVEQLQVRQSV
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
ALVRDRAVAALARLARLAAEHEATVMAGRSHNVAAQATTLGKRFATVADEMLVALDRVED
HHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLVRYPLRGIKGPMGTAQDMLDLLDGDAAKLAELEQRVAAHLGFERVLTSVGQVYPRSLD
HHHHHCHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCC
LDVVSALVQLVSGPSNLATTIRLMAGNELVTEGFKEGQVGSSAMPHKMNSRSAERVNGLS
HHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
VVLRGHLSMVSELAGDQWNEGDVSDSVVRRVALPDAFFAADGLFETFLTILDEFGAFPAV
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHH
IQRELDRYLPFLATTKVLMAAVRNGVGREAAHESIKEAAVGTALDMRRGQADNDVFARLA
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
ADTRLGLTSDQLASLVAEPITFTGAAVAQVQAVVRRVAEVTDRHPAAAAYAPGDIL
HHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]