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The map label for this gene is thcD [H]

Identifier: 119718501

GI number: 119718501

Start: 4524522

End: 4525733

Strand: Reverse

Name: thcD [H]

Synonym: Noca_4282

Alternate gene names: 119718501

Gene position: 4525733-4524522 (Counterclockwise)

Preceding gene: 119718502

Following gene: 119718500

Centisome position: 90.77

GC content: 75.0

Gene sequence:

>1212_bases
ATGAGCGACCCCACGGTGGTCATCATCGGCGGCGGCCAGGCCGGCGCGGTCGCCACGCGCGCCCTGCGCCGCAAGGGTCA
CGCCGGGCGGATCGTCCTGGTGGGCGAGGAGCCGCTGCGGCCCTACCAGCGCCCGCCGCTCTCGAAGGAGTACCTCGCGA
CCGGTGACGCCTCCGGGCTCGACCTGCTCCCCGAGACCTGGACCGAGGAGAACGACGTCGAGGTGCGCACCGGCGCCCGA
GCCCTGAAGATCAGCGCCGCCGACGCCGGCGTGCTCCTCGACGACGGTACGACGCTGCCCGCGGACCGGGTGCTGATCGC
CACGGGCGGCCGCCCGCGGCGGCTCGCCGACGCCGACGGCGACCGGGTGCGCTACCTGCGCACCCGCGCGGACGCCGACG
CCCTCGACGACCTGATGACGCCGGGCGGCCGCCTGGTCGTCGTCGGCGGCGGGTTCGTCGGCGCCGAGGTCGCCTCGACC
GCGCTGGCCCGCGGGCTGCACGTGACCGTGCTCGAGGCGGCCCCGGTGCCGATGGGACGCGCACTCGGCGCGGAGCTCGG
CGCCCGCTGCGGCCGGCTGCAGGTCGCCGCGGGCGTCGACCTCCGGCTCGACGCTGCGGTCGGGCGGGTGCGGCAGGAGG
GCGACGAGGTGGTCGTGGAGACCTCGACCGGCACCGTCGCCGGTGACTGCGTGCTGGTCGGGATCGGCATGGTGCCCAAC
GACGAGGTCGCCCGGGACTCGGGCATCGAGACCGGCAACGGGATCCTGGTGGACGAGCACTGCCGCACGAGCCTGCCGAA
CGTCTTCGCCGCTGGCGACGTCGCCAACCACCACCACCCGCTGTTCGGGACCCGGATGCGCGTCGAGCACTTCGACAACG
CCAGCCGGCAGGCCGCGGTGGCGGCCGACAACCTGCTCGGCCGGAGCACCGTCTTCGACGACCCGCACTGGTTCTGGTCC
GACCAGTACGGCGCGAACCTGCAGTTCGTCGGCCACGCCGAGGGCGCGGACCAGGTCGTCACCCGCGGGGACCCCGAGTC
CGAGAGCTGGTCGGCCTTCTTCCTCCGCGAGGGCCGGGTGCGCGCCGCCTTCGCCCTGAACCAGGGCGAGGACGTCGCCG
TCGCTCGCGAGCTGATCGCGCTCGGGATCGCCGTACCGCCGGGGACGCTCGCCGACCCCGACGCCGACCTGATGGAGGCC
TTCGATGAGTGA

Upstream 100 bases:

>100_bases
GGGAGCCCGACGGCCAGGACGGCTGCCAGCCGACGTACCCGGTGCGGGTGGCGGAGGACGGGACGATCTCGGTCGCCCTC
GAGACCTCGGTGGTGACCCG

Downstream 100 bases:

>100_bases
ACCGGAGCTGCGGGGCAACTTCGTCGGCGGGCACTGGGTGGGCGCCTCGGGCGGGCGCACCTTCGAGCGCCGCAACCCCG
CCGACCCCGCCGACGTCGTC

Product: FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MSDPTVVIIGGGQAGAVATRALRRKGHAGRIVLVGEEPLRPYQRPPLSKEYLATGDASGLDLLPETWTEENDVEVRTGAR
ALKISAADAGVLLDDGTTLPADRVLIATGGRPRRLADADGDRVRYLRTRADADALDDLMTPGGRLVVVGGGFVGAEVAST
ALARGLHVTVLEAAPVPMGRALGAELGARCGRLQVAAGVDLRLDAAVGRVRQEGDEVVVETSTGTVAGDCVLVGIGMVPN
DEVARDSGIETGNGILVDEHCRTSLPNVFAAGDVANHHHPLFGTRMRVEHFDNASRQAAVAADNLLGRSTVFDDPHWFWS
DQYGANLQFVGHAEGADQVVTRGDPESESWSAFFLREGRVRAAFALNQGEDVAVARELIALGIAVPPGTLADPDADLMEA
FDE

Sequences:

>Translated_403_residues
MSDPTVVIIGGGQAGAVATRALRRKGHAGRIVLVGEEPLRPYQRPPLSKEYLATGDASGLDLLPETWTEENDVEVRTGAR
ALKISAADAGVLLDDGTTLPADRVLIATGGRPRRLADADGDRVRYLRTRADADALDDLMTPGGRLVVVGGGFVGAEVAST
ALARGLHVTVLEAAPVPMGRALGAELGARCGRLQVAAGVDLRLDAAVGRVRQEGDEVVVETSTGTVAGDCVLVGIGMVPN
DEVARDSGIETGNGILVDEHCRTSLPNVFAAGDVANHHHPLFGTRMRVEHFDNASRQAAVAADNLLGRSTVFDDPHWFWS
DQYGANLQFVGHAEGADQVVTRGDPESESWSAFFLREGRVRAAFALNQGEDVAVARELIALGIAVPPGTLADPDADLMEA
FDE
>Mature_402_residues
SDPTVVIIGGGQAGAVATRALRRKGHAGRIVLVGEEPLRPYQRPPLSKEYLATGDASGLDLLPETWTEENDVEVRTGARA
LKISAADAGVLLDDGTTLPADRVLIATGGRPRRLADADGDRVRYLRTRADADALDDLMTPGGRLVVVGGGFVGAEVASTA
LARGLHVTVLEAAPVPMGRALGAELGARCGRLQVAAGVDLRLDAAVGRVRQEGDEVVVETSTGTVAGDCVLVGIGMVPND
EVARDSGIETGNGILVDEHCRTSLPNVFAAGDVANHHHPLFGTRMRVEHFDNASRQAAVAADNLLGRSTVFDDPHWFWSD
QYGANLQFVGHAEGADQVVTRGDPESESWSAFFLREGRVRAAFALNQGEDVAVARELIALGIAVPPGTLADPDADLMEAF
DE

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=393, Percent_Identity=32.3155216284987, Blast_Score=166, Evalue=2e-41,
Organism=Homo sapiens, GI226437568, Length=393, Percent_Identity=32.3155216284987, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI65787454, Length=393, Percent_Identity=32.3155216284987, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI4757732, Length=340, Percent_Identity=27.9411764705882, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI22202629, Length=340, Percent_Identity=27.9411764705882, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI195927006, Length=175, Percent_Identity=29.7142857142857, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1788892, Length=400, Percent_Identity=36.5, Blast_Score=214, Evalue=7e-57,
Organism=Escherichia coli, GI1789765, Length=322, Percent_Identity=27.9503105590062, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1789065, Length=389, Percent_Identity=21.8508997429306, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17559934, Length=402, Percent_Identity=31.0945273631841, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI32564386, Length=338, Percent_Identity=28.698224852071, Blast_Score=114, Evalue=8e-26,
Organism=Drosophila melanogaster, GI24639257, Length=398, Percent_Identity=29.1457286432161, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI281359715, Length=398, Percent_Identity=29.1457286432161, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI281359713, Length=398, Percent_Identity=29.1457286432161, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24639250, Length=398, Percent_Identity=29.1457286432161, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI18543267, Length=398, Percent_Identity=29.1457286432161, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24639252, Length=398, Percent_Identity=29.1457286432161, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24585130, Length=396, Percent_Identity=25.7575757575758, Blast_Score=124, Evalue=8e-29,
Organism=Drosophila melanogaster, GI28573993, Length=356, Percent_Identity=28.0898876404494, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24581020, Length=356, Percent_Identity=28.0898876404494, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 42411; Mature: 42280

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDPTVVIIGGGQAGAVATRALRRKGHAGRIVLVGEEPLRPYQRPPLSKEYLATGDASGL
CCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHEECCCCCCC
DLLPETWTEENDVEVRTGARALKISAADAGVLLDDGTTLPADRVLIATGGRPRRLADADG
CCCCCCCCCCCCEEEECCCEEEEEEECCCCEEEECCCCCCCCEEEEEECCCCCCCCCCCC
DRVRYLRTRADADALDDLMTPGGRLVVVGGGFVGAEVASTALARGLHVTVLEAAPVPMGR
CEEEEEEHHCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCCCCH
ALGAELGARCGRLQVAAGVDLRLDAAVGRVRQEGDEVVVETSTGTVAGDCVLVGIGMVPN
HHHHHHHCCCCEEEEECCCCEEEHHHHHHHHCCCCEEEEEECCCCEECCEEEEEECCCCC
DEVARDSGIETGNGILVDEHCRTSLPNVFAAGDVANHHHPLFGTRMRVEHFDNASRQAAV
CHHHHHCCCCCCCCEEEEHHHHCCCCCEEEECCCCCCCCCCCCCEEEEHHCCCCCHHHHH
AADNLLGRSTVFDDPHWFWSDQYGANLQFVGHAEGADQVVTRGDPESESWSAFFLREGRV
HHHHHCCCCCCCCCCCEEECCCCCCCEEEEECCCCCCCCEECCCCCCCCCEEEEEECCCE
RAAFALNQGEDVAVARELIALGIAVPPGTLADPDADLMEAFDE
EEEEEECCCCCHHHHHHHHHHEEECCCCCCCCCCHHHHHHHCC
>Mature Secondary Structure 
SDPTVVIIGGGQAGAVATRALRRKGHAGRIVLVGEEPLRPYQRPPLSKEYLATGDASGL
CCCEEEEEECCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHEECCCCCCC
DLLPETWTEENDVEVRTGARALKISAADAGVLLDDGTTLPADRVLIATGGRPRRLADADG
CCCCCCCCCCCCEEEECCCEEEEEEECCCCEEEECCCCCCCCEEEEEECCCCCCCCCCCC
DRVRYLRTRADADALDDLMTPGGRLVVVGGGFVGAEVASTALARGLHVTVLEAAPVPMGR
CEEEEEEHHCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCCCCH
ALGAELGARCGRLQVAAGVDLRLDAAVGRVRQEGDEVVVETSTGTVAGDCVLVGIGMVPN
HHHHHHHCCCCEEEEECCCCEEEHHHHHHHHCCCCEEEEEECCCCEECCEEEEEECCCCC
DEVARDSGIETGNGILVDEHCRTSLPNVFAAGDVANHHHPLFGTRMRVEHFDNASRQAAV
CHHHHHCCCCCCCCEEEEHHHHCCCCCEEEECCCCCCCCCCCCCEEEEHHCCCCCHHHHH
AADNLLGRSTVFDDPHWFWSDQYGANLQFVGHAEGADQVVTRGDPESESWSAFFLREGRV
HHHHHCCCCCCCCCCCEEECCCCCCCEEEEECCCCCCCCEECCCCCCCCCEEEEEECCCE
RAAFALNQGEDVAVARELIALGIAVPPGTLADPDADLMEAFDE
EEEEEECCCCCHHHHHHHHHHEEECCCCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]