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The map label for this gene is thlA [H]

Identifier: 119718361

GI number: 119718361

Start: 4373723

End: 4374916

Strand: Reverse

Name: thlA [H]

Synonym: Noca_4142

Alternate gene names: 119718361

Gene position: 4374916-4373723 (Counterclockwise)

Preceding gene: 119718363

Following gene: 119718360

Centisome position: 87.75

GC content: 74.2

Gene sequence:

>1194_bases
GTGACCCGGGCCGAGACCCGTGATGACGCGATCGTGGTGGTGGACGGGGCGCGGACCCCGATCGGCAGCTTCGGCGGGAT
GTTGAGGGACGTTCCCGCCCACGAGCTCGGCGCGACGGCGGCCCGCGAGGCACTCCGCCGCGCCGGCGTCGAGGCCGAGG
CGATCGACGAGGTCATCATGGGCTGCATCGGCCAGGTCGGGCCGGACGCCTACAACGCGCGCCGTGTCGCCCTGGCCGCG
GGGCTCCCCGACCGGACCCCGGCGTACACCGTCAACCGCCTGTGCGGCTCCGGCCTCCAGGCCGTGTGGTCGGCGGCCAT
GCAGATCCGCTGGGGCGCCGCGGAGCTCGCGCTCGCCGGCGGGGACGAGTCGATGACCCGGATGCCGTTCTACGACTTCG
GCGCGCGCGCCGGCTACAAGCTGGGCGACCGCGCGCTCGTCGACGGCACCGTGATGATGCTGACCGACCCGTTCCACGGC
ATCCACATGGGCGTCACGGCCGAGCGGGTCGCCGAGAAGTACGGCGTGACCCGCGCGGAGCAGGACGAGTTCGCGGCGGA
GTCGCAGCGGCGGGCGGCGACCCCCGAGGCACGGGCCGCGTTCGCCGAGGAGATCGTCCCGGTCGAGGTCGGCGGCCGCC
GGCCGTTCACCGCCAGCGAGGACGAGCACCCCAAGCCCGACACCACCGTCGAGACCCTGGCCCGGCTCCGTCCCGCGTTC
GCCGAGGGCGGCACCGTGACGGCCGGCAACGCCTCCGGGATCAACGACGGCGGCGCCGCGGTGGTGGTGGCGAGCAGCCG
CGTGGCCGCCGACCGCGGCCTCACGCCGCTGGTAGAGCTGGTGTCGGTGGCGACAGCAGCCCTGGAACCCGAGCTGATGG
GCTACGCCCCGGTCCTCGCGCTGCGCTCGCTCTTCGAACAGACGGGTACGACGCCGGGCGACATCGGGATCATCGAGCTG
AACGAGGCCTTCGCCGCGCAGGCGGTGGCATGCGTGCGCGACGCCGGCCTCGACCCCGAGCGGGTCAACCCGTACGGCGG
TGCCATCGCGCTCGGCCACCCGGTCGGCGCGACCGGCGCGAACCTGACGCTGCGAGTCGCCAAGGACCTGGTCCGCCGCG
ATCTCGAGCTCGGCATCGTGACGATGTGCATCGGCGGCGGGCAGGCCCTCGCCGCACTCTTCCGGAGAGTGTGA

Upstream 100 bases:

>100_bases
GGCTTCGAGGAAGAGGGTGGCGGTCCAGATAGTAGGACGTCCTAGTAAAAAGTAGTAGGGCGCCCTAGTAATTCCCTACT
GTGTCCCCGTGACCAGTGCT

Downstream 100 bases:

>100_bases
TGAGCGGGACCGTGCGCTACGAGCTCACCGACGGCGTCGCCCACCTGGTCCTCGACCGTCCCGACGCCGCCAACGCGATC
GACATGGACACCGCGCTCGC

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MTRAETRDDAIVVVDGARTPIGSFGGMLRDVPAHELGATAAREALRRAGVEAEAIDEVIMGCIGQVGPDAYNARRVALAA
GLPDRTPAYTVNRLCGSGLQAVWSAAMQIRWGAAELALAGGDESMTRMPFYDFGARAGYKLGDRALVDGTVMMLTDPFHG
IHMGVTAERVAEKYGVTRAEQDEFAAESQRRAATPEARAAFAEEIVPVEVGGRRPFTASEDEHPKPDTTVETLARLRPAF
AEGGTVTAGNASGINDGGAAVVVASSRVAADRGLTPLVELVSVATAALEPELMGYAPVLALRSLFEQTGTTPGDIGIIEL
NEAFAAQAVACVRDAGLDPERVNPYGGAIALGHPVGATGANLTLRVAKDLVRRDLELGIVTMCIGGGQALAALFRRV

Sequences:

>Translated_397_residues
MTRAETRDDAIVVVDGARTPIGSFGGMLRDVPAHELGATAAREALRRAGVEAEAIDEVIMGCIGQVGPDAYNARRVALAA
GLPDRTPAYTVNRLCGSGLQAVWSAAMQIRWGAAELALAGGDESMTRMPFYDFGARAGYKLGDRALVDGTVMMLTDPFHG
IHMGVTAERVAEKYGVTRAEQDEFAAESQRRAATPEARAAFAEEIVPVEVGGRRPFTASEDEHPKPDTTVETLARLRPAF
AEGGTVTAGNASGINDGGAAVVVASSRVAADRGLTPLVELVSVATAALEPELMGYAPVLALRSLFEQTGTTPGDIGIIEL
NEAFAAQAVACVRDAGLDPERVNPYGGAIALGHPVGATGANLTLRVAKDLVRRDLELGIVTMCIGGGQALAALFRRV
>Mature_396_residues
TRAETRDDAIVVVDGARTPIGSFGGMLRDVPAHELGATAAREALRRAGVEAEAIDEVIMGCIGQVGPDAYNARRVALAAG
LPDRTPAYTVNRLCGSGLQAVWSAAMQIRWGAAELALAGGDESMTRMPFYDFGARAGYKLGDRALVDGTVMMLTDPFHGI
HMGVTAERVAEKYGVTRAEQDEFAAESQRRAATPEARAAFAEEIVPVEVGGRRPFTASEDEHPKPDTTVETLARLRPAFA
EGGTVTAGNASGINDGGAAVVVASSRVAADRGLTPLVELVSVATAALEPELMGYAPVLALRSLFEQTGTTPGDIGIIELN
EAFAAQAVACVRDAGLDPERVNPYGGAIALGHPVGATGANLTLRVAKDLVRRDLELGIVTMCIGGGQALAALFRRV

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=393, Percent_Identity=41.7302798982188, Blast_Score=299, Evalue=3e-81,
Organism=Homo sapiens, GI167614485, Length=388, Percent_Identity=40.7216494845361, Blast_Score=289, Evalue=3e-78,
Organism=Homo sapiens, GI4501853, Length=396, Percent_Identity=38.8888888888889, Blast_Score=249, Evalue=3e-66,
Organism=Homo sapiens, GI4557237, Length=393, Percent_Identity=37.4045801526718, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI4504327, Length=423, Percent_Identity=31.6784869976359, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI194353979, Length=386, Percent_Identity=31.0880829015544, Blast_Score=150, Evalue=2e-36,
Organism=Escherichia coli, GI1788554, Length=392, Percent_Identity=46.9387755102041, Blast_Score=320, Evalue=8e-89,
Organism=Escherichia coli, GI1787663, Length=397, Percent_Identity=45.5919395465995, Blast_Score=306, Evalue=2e-84,
Organism=Escherichia coli, GI87082165, Length=388, Percent_Identity=42.2680412371134, Blast_Score=288, Evalue=4e-79,
Organism=Escherichia coli, GI48994986, Length=405, Percent_Identity=40.9876543209877, Blast_Score=263, Evalue=1e-71,
Organism=Escherichia coli, GI1788683, Length=417, Percent_Identity=31.89448441247, Blast_Score=169, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI133906874, Length=390, Percent_Identity=42.3076923076923, Blast_Score=303, Evalue=1e-82,
Organism=Caenorhabditis elegans, GI25147385, Length=397, Percent_Identity=35.5163727959698, Blast_Score=255, Evalue=2e-68,
Organism=Caenorhabditis elegans, GI17535921, Length=400, Percent_Identity=38.25, Blast_Score=246, Evalue=1e-65,
Organism=Caenorhabditis elegans, GI17551802, Length=420, Percent_Identity=29.2857142857143, Blast_Score=167, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI17535917, Length=403, Percent_Identity=29.7766749379653, Blast_Score=164, Evalue=8e-41,
Organism=Saccharomyces cerevisiae, GI6325229, Length=396, Percent_Identity=39.6464646464646, Blast_Score=249, Evalue=6e-67,
Organism=Saccharomyces cerevisiae, GI6322031, Length=404, Percent_Identity=37.3762376237624, Blast_Score=233, Evalue=4e-62,
Organism=Drosophila melanogaster, GI24655093, Length=389, Percent_Identity=43.9588688946015, Blast_Score=318, Evalue=3e-87,
Organism=Drosophila melanogaster, GI17648125, Length=393, Percent_Identity=41.4758269720102, Blast_Score=278, Evalue=4e-75,
Organism=Drosophila melanogaster, GI24640423, Length=391, Percent_Identity=39.386189258312, Blast_Score=258, Evalue=4e-69,
Organism=Drosophila melanogaster, GI17137578, Length=430, Percent_Identity=31.3953488372093, Blast_Score=182, Evalue=4e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 41433; Mature: 41302

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRAETRDDAIVVVDGARTPIGSFGGMLRDVPAHELGATAAREALRRAGVEAEAIDEVIM
CCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHH
GCIGQVGPDAYNARRVALAAGLPDRTPAYTVNRLCGSGLQAVWSAAMQIRWGAAELALAG
HHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCHHEEEEEC
GDESMTRMPFYDFGARAGYKLGDRALVDGTVMMLTDPFHGIHMGVTAERVAEKYGVTRAE
CCCCCEECCCHHCCCCCCCCCCCEEEECCEEEEEECCCCCEEECCHHHHHHHHHCCCCCC
QDEFAAESQRRAATPEARAAFAEEIVPVEVGGRRPFTASEDEHPKPDTTVETLARLRPAF
HHHHHHHHHHHCCCCHHHHHHHHHCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
AEGGTVTAGNASGINDGGAAVVVASSRVAADRGLTPLVELVSVATAALEPELMGYAPVLA
CCCCEEECCCCCCCCCCCCEEEEECCHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHH
LRSLFEQTGTTPGDIGIIELNEAFAAQAVACVRDAGLDPERVNPYGGAIALGHPVGATGA
HHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHCCCCHHHCCCCCCEEEECCCCCCCCC
NLTLRVAKDLVRRDLELGIVTMCIGGGQALAALFRRV
CCHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHCC
>Mature Secondary Structure 
TRAETRDDAIVVVDGARTPIGSFGGMLRDVPAHELGATAAREALRRAGVEAEAIDEVIM
CCCCCCCCEEEEECCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHH
GCIGQVGPDAYNARRVALAAGLPDRTPAYTVNRLCGSGLQAVWSAAMQIRWGAAELALAG
HHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCHHEEEEEC
GDESMTRMPFYDFGARAGYKLGDRALVDGTVMMLTDPFHGIHMGVTAERVAEKYGVTRAE
CCCCCEECCCHHCCCCCCCCCCCEEEECCEEEEEECCCCCEEECCHHHHHHHHHCCCCCC
QDEFAAESQRRAATPEARAAFAEEIVPVEVGGRRPFTASEDEHPKPDTTVETLARLRPAF
HHHHHHHHHHHCCCCHHHHHHHHHCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
AEGGTVTAGNASGINDGGAAVVVASSRVAADRGLTPLVELVSVATAALEPELMGYAPVLA
CCCCEEECCCCCCCCCCCCEEEEECCHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHH
LRSLFEQTGTTPGDIGIIELNEAFAAQAVACVRDAGLDPERVNPYGGAIALGHPVGATGA
HHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHCCCCHHHCCCCCCEEEECCCCCCCCC
NLTLRVAKDLVRRDLELGIVTMCIGGGQALAALFRRV
CCHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]