BacMap

Definition	Nocardioides sp. JS614 chromosome, complete genome.
Accession	NC_008699
Length	4,985,871

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The map label for this gene is paaG [C]

Identifier: 119718329

GI number: 119718329

Start: 4341650

End: 4342447

Strand: Reverse

Name: paaG [C]

Synonym: Noca_4110

Alternate gene names: 119718329

Gene position: 4342447-4341650 (Counterclockwise)

Preceding gene: 119718331

Following gene: 119718328

Centisome position: 87.1

GC content: 71.93

Gene sequence:

>798_bases
ATGCCTCATGACGTCGTGTTGGTGGAGCGCCGCGACGGTGTCCGGCGGGTGATCTTGAACCGCCCCGAGGTGCTCAACGC
CTACGACACCGCCTTGTGCCAGCAACTCGGCGCGGCCTTGCTGGACTTCCAGCGCTGCGACGAGGACCGGGTGCTGGTGC
TCTCCGGTGCCGGCCGCGCGTTCTGCGTCGGCGGCGACGTCCGCAGCGAGGCCGAGGCGGTCGAGGGGGAGGAGCGCCAG
CTCGGGCACGGCATGGTGATGCGCGAGGGCATGCACTCGGTGCACCGCCTGCTGCACGCCCTGGACAAGCCGGTGATCGC
GCTGATCCACGGCCATGCGGTCGCGGGCGGCCTCTCGTTGGCGCTGCTCTGTGACTTCCGGATCGCCGCGCAGAGTGCGC
GCCTCGGCGACACGAGCGGTCGGGTCGGACTGCTCCCCGACGAGGGAGGCGCCTGGTTGTTCCCGCGGGCGATGGGCCAC
GACGCGGCGCTGCGAATGACGCTGCTCGGCGAGGTCTACGACGCGGCGGAGGCGCACCGGCTGGGGCTGGTCACCGAGGT
GGTCCCCGACGACCGGCTCCAGGAGCGGGGCGCGGAGCTTGCGGCGCAGATCGCCGCGAAGGCGCCGCTCGCGGTGCGGA
TGGCCAAGCGGATGATGCGGCGCTCCCGTGAGCAGACCTTCGAGGAGTCCCTCGTCGAGGCGGAGTACGCCGTCGAGATC
GTCAACCGCAGCGACGACGTGCGCGAGGGCGTCGAGGCCTTCGTCGCCAAGCGGACGCCGACGTTCACCGGCCACTGA

Upstream 100 bases:

>100_bases
GGATCCTGGCCGGCACTGAGCGTGCCCGAGCCTAGACGTCGCTTGAAGGTGACGTCAAAGTCAATTATGCTTCCCGTCAT
GTCCACGGGAGGGTCTCAGC

Downstream 100 bases:

>100_bases
TCCCGGATGGAGCCGCCGCACGCCTGGTCCTTCGACGACTTCGAGGCGGGCCAGGTGTTCACCGGCCAGGGCCGCACGAT
CACCGAGTCCGACCTCGTCT

Product: short chain enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MPHDVVLVERRDGVRRVILNRPEVLNAYDTALCQQLGAALLDFQRCDEDRVLVLSGAGRAFCVGGDVRSEAEAVEGEERQ
LGHGMVMREGMHSVHRLLHALDKPVIALIHGHAVAGGLSLALLCDFRIAAQSARLGDTSGRVGLLPDEGGAWLFPRAMGH
DAALRMTLLGEVYDAAEAHRLGLVTEVVPDDRLQERGAELAAQIAAKAPLAVRMAKRMMRRSREQTFEESLVEAEYAVEI
VNRSDDVREGVEAFVAKRTPTFTGH

Sequences:

>Translated_265_residues
MPHDVVLVERRDGVRRVILNRPEVLNAYDTALCQQLGAALLDFQRCDEDRVLVLSGAGRAFCVGGDVRSEAEAVEGEERQ
LGHGMVMREGMHSVHRLLHALDKPVIALIHGHAVAGGLSLALLCDFRIAAQSARLGDTSGRVGLLPDEGGAWLFPRAMGH
DAALRMTLLGEVYDAAEAHRLGLVTEVVPDDRLQERGAELAAQIAAKAPLAVRMAKRMMRRSREQTFEESLVEAEYAVEI
VNRSDDVREGVEAFVAKRTPTFTGH
>Mature_264_residues
PHDVVLVERRDGVRRVILNRPEVLNAYDTALCQQLGAALLDFQRCDEDRVLVLSGAGRAFCVGGDVRSEAEAVEGEERQL
GHGMVMREGMHSVHRLLHALDKPVIALIHGHAVAGGLSLALLCDFRIAAQSARLGDTSGRVGLLPDEGGAWLFPRAMGHD
AALRMTLLGEVYDAAEAHRLGLVTEVVPDDRLQERGAELAAQIAAKAPLAVRMAKRMMRRSREQTFEESLVEAEYAVEIV
NRSDDVREGVEAFVAKRTPTFTGH

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=263, Percent_Identity=30.4182509505703, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI37594469, Length=189, Percent_Identity=30.1587301587302, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI37594471, Length=189, Percent_Identity=30.1587301587302, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI45643119, Length=261, Percent_Identity=27.5862068965517, Blast_Score=95, Evalue=6e-20,
Organism=Homo sapiens, GI260275230, Length=261, Percent_Identity=27.5862068965517, Blast_Score=95, Evalue=6e-20,
Organism=Homo sapiens, GI260274832, Length=261, Percent_Identity=27.5862068965517, Blast_Score=95, Evalue=7e-20,
Organism=Homo sapiens, GI31542718, Length=256, Percent_Identity=27.734375, Blast_Score=91, Evalue=8e-19,
Organism=Homo sapiens, GI70995211, Length=261, Percent_Identity=28.3524904214559, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI4502327, Length=266, Percent_Identity=29.6992481203008, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI213417737, Length=237, Percent_Identity=24.4725738396624, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI157694516, Length=237, Percent_Identity=24.4725738396624, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI150378541, Length=261, Percent_Identity=29.1187739463602, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI4757966, Length=251, Percent_Identity=22.7091633466135, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI51538581, Length=251, Percent_Identity=22.7091633466135, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI25453481, Length=216, Percent_Identity=24.0740740740741, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI51538573, Length=216, Percent_Identity=24.0740740740741, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI68989263, Length=195, Percent_Identity=33.3333333333333, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI4757968, Length=183, Percent_Identity=26.2295081967213, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI49355787, Length=183, Percent_Identity=26.2295081967213, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI221136756, Length=227, Percent_Identity=24.2290748898678, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI221136753, Length=227, Percent_Identity=24.2290748898678, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI20127408, Length=256, Percent_Identity=28.515625, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI221307494, Length=227, Percent_Identity=24.2290748898678, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1787660, Length=263, Percent_Identity=36.5019011406844, Blast_Score=144, Evalue=4e-36,
Organism=Escherichia coli, GI1787659, Length=259, Percent_Identity=33.2046332046332, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI221142681, Length=260, Percent_Identity=32.3076923076923, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1788597, Length=267, Percent_Identity=30.3370786516854, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1790281, Length=184, Percent_Identity=30.9782608695652, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1788682, Length=226, Percent_Identity=33.1858407079646, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI87082183, Length=269, Percent_Identity=25.6505576208178, Blast_Score=76, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25145438, Length=252, Percent_Identity=31.3492063492063, Blast_Score=127, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17554946, Length=265, Percent_Identity=32.4528301886792, Blast_Score=124, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17540714, Length=220, Percent_Identity=31.8181818181818, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17536985, Length=242, Percent_Identity=30.9917355371901, Blast_Score=104, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17549921, Length=224, Percent_Identity=26.7857142857143, Blast_Score=103, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17534483, Length=263, Percent_Identity=28.1368821292776, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17535521, Length=260, Percent_Identity=26.5384615384615, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17560910, Length=263, Percent_Identity=29.6577946768061, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI25144157, Length=200, Percent_Identity=30, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI25144160, Length=200, Percent_Identity=30, Blast_Score=84, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17540306, Length=255, Percent_Identity=26.6666666666667, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17558304, Length=190, Percent_Identity=26.8421052631579, Blast_Score=70, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI25141363, Length=242, Percent_Identity=28.5123966942149, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17508951, Length=241, Percent_Identity=28.2157676348548, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI25144276, Length=241, Percent_Identity=28.2157676348548, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17508953, Length=241, Percent_Identity=28.2157676348548, Blast_Score=68, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=206, Percent_Identity=27.1844660194175, Blast_Score=97, Evalue=4e-21,
Organism=Drosophila melanogaster, GI20129971, Length=247, Percent_Identity=31.9838056680162, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24653477, Length=247, Percent_Identity=31.9838056680162, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24653139, Length=275, Percent_Identity=32, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI21357171, Length=267, Percent_Identity=32.2097378277154, Blast_Score=110, Evalue=8e-25,
Organism=Drosophila melanogaster, GI24650670, Length=258, Percent_Identity=32.9457364341085, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI19922422, Length=261, Percent_Identity=24.5210727969349, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI28571729, Length=181, Percent_Identity=29.8342541436464, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI19920382, Length=259, Percent_Identity=27.027027027027, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI28571730, Length=181, Percent_Identity=29.8342541436464, Blast_Score=82, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28959; Mature: 28827

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHDVVLVERRDGVRRVILNRPEVLNAYDTALCQQLGAALLDFQRCDEDRVLVLSGAGRA
CCCCEEEEECCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCE
FCVGGDVRSEAEAVEGEERQLGHGMVMREGMHSVHRLLHALDKPVIALIHGHAVAGGLSL
EEECCCHHHHHHHHCCHHHHHHCHHHHHHHHHHHHHHHHHHCCCCEEHHHCHHHHHHHHH
ALLCDFRIAAQSARLGDTSGRVGLLPDEGGAWLFPRAMGHDAALRMTLLGEVYDAAEAHR
HHHHHHHHHHHHCCCCCCCCCEEECCCCCCCEECCHHCCCHHHHHHHHHHHHHHHHHHHH
LGLVTEVVPDDRLQERGAELAAQIAAKAPLAVRMAKRMMRRSREQTFEESLVEAEYAVEI
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VNRSDDVREGVEAFVAKRTPTFTGH
HCCCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
PHDVVLVERRDGVRRVILNRPEVLNAYDTALCQQLGAALLDFQRCDEDRVLVLSGAGRA
CCCEEEEECCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCE
FCVGGDVRSEAEAVEGEERQLGHGMVMREGMHSVHRLLHALDKPVIALIHGHAVAGGLSL
EEECCCHHHHHHHHCCHHHHHHCHHHHHHHHHHHHHHHHHHCCCCEEHHHCHHHHHHHHH
ALLCDFRIAAQSARLGDTSGRVGLLPDEGGAWLFPRAMGHDAALRMTLLGEVYDAAEAHR
HHHHHHHHHHHHCCCCCCCCCEEECCCCCCCEECCHHCCCHHHHHHHHHHHHHHHHHHHH
LGLVTEVVPDDRLQERGAELAAQIAAKAPLAVRMAKRMMRRSREQTFEESLVEAEYAVEI
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VNRSDDVREGVEAFVAKRTPTFTGH
HCCCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA