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The map label for this gene is dapF [H]

Identifier: 119718061

GI number: 119718061

Start: 4061990

End: 4062829

Strand: Reverse

Name: dapF [H]

Synonym: Noca_3841

Alternate gene names: 119718061

Gene position: 4062829-4061990 (Counterclockwise)

Preceding gene: 119718062

Following gene: 119718060

Centisome position: 81.49

GC content: 74.4

Gene sequence:

>840_bases
GTGACCACCACGCCCGCCCCGACAGCGGCTGACGGCTACCCGTTCCTCAAGGGTCACGGCACCGAGAACGACTTCGTGCT
GCTGCCCGACCTCGACGGCAGCCTCCACGGCGACCTGTCCGCCGACCGGGTCCGCGCGTTGTGCGACCGGCACGCCGGCA
TCGGTGGCGACGGGGTGCTGCGCGTGCTGCGCACCGACGCCGGGTGGTTCATGGACTACCGCAACGCCGACGGCTCGATC
GCGGAGATGTGCGGCAACGGGATCCGGGTCTTCGTGCGCCACCTGGTCGAGGAGGGGCTGGCCGATCCCGGTCAGCCGAT
CGAGGTCGACACCCGCGACGGGGTCAAGGTGCTCACCGTCGACGGCGACCTGATCACCGCGGACATGGGCCGGCCCCGGG
TGCTCGGCGAGACCGTGGTCGGCGTCGGCACGGTCAGCTGGCCCGCGCGCCACGTCGACATGGGCAACCCGCACGCGGTG
GCGTTCGTGGACTCGCTGGACCCGCACGGACCCGTGGGCCCGCTGCTCGACCCGCCCAGCCGCGACCCGGAGACCTTCCC
GGACGGGGCGAACGTGGAGTTCGTCGTACGCCGGGGCGAGCGGCACGTCGCGATGCGTGTGCATGAGCGCGGCTCGGGGG
AGACCCGGTCCTGCGGCACCGGCGCCTGCGCGGTGATGGTGGCGGCCGCGGTGGCCGACGGCGCCCGCCCCTCCGGCGCC
GACGTCGCCTACCGCGTCGACGTCCCCGGCGGCACCCTCACGGTCACCTGGACCGCCGACGACCGGGTCCTGCTCACCGG
CCCCGCCGTCATCGTCGCCCGGGGCACCACCGTGCTCTGA

Upstream 100 bases:

>100_bases
AGCTCCGGCTCGAGGAGCACGCCCGCAACCGCGAGTTCCTGTGCGCCCGGTACGCCGTGCTGCCGGGGCCTGACGCGGAG
CCGGCCAGTACCGTGGGGGC

Downstream 100 bases:

>100_bases
GCGGCGAACCCGGCGACCGCTCGGACCCGCACCGTGACAGTCCCACTGTGAGCATGGTTATGCTCGCGCGCATGGACATC
AACGGAGCCAGTGCCATCGT

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MTTTPAPTAADGYPFLKGHGTENDFVLLPDLDGSLHGDLSADRVRALCDRHAGIGGDGVLRVLRTDAGWFMDYRNADGSI
AEMCGNGIRVFVRHLVEEGLADPGQPIEVDTRDGVKVLTVDGDLITADMGRPRVLGETVVGVGTVSWPARHVDMGNPHAV
AFVDSLDPHGPVGPLLDPPSRDPETFPDGANVEFVVRRGERHVAMRVHERGSGETRSCGTGACAVMVAAAVADGARPSGA
DVAYRVDVPGGTLTVTWTADDRVLLTGPAVIVARGTTVL

Sequences:

>Translated_279_residues
MTTTPAPTAADGYPFLKGHGTENDFVLLPDLDGSLHGDLSADRVRALCDRHAGIGGDGVLRVLRTDAGWFMDYRNADGSI
AEMCGNGIRVFVRHLVEEGLADPGQPIEVDTRDGVKVLTVDGDLITADMGRPRVLGETVVGVGTVSWPARHVDMGNPHAV
AFVDSLDPHGPVGPLLDPPSRDPETFPDGANVEFVVRRGERHVAMRVHERGSGETRSCGTGACAVMVAAAVADGARPSGA
DVAYRVDVPGGTLTVTWTADDRVLLTGPAVIVARGTTVL
>Mature_278_residues
TTTPAPTAADGYPFLKGHGTENDFVLLPDLDGSLHGDLSADRVRALCDRHAGIGGDGVLRVLRTDAGWFMDYRNADGSIA
EMCGNGIRVFVRHLVEEGLADPGQPIEVDTRDGVKVLTVDGDLITADMGRPRVLGETVVGVGTVSWPARHVDMGNPHAVA
FVDSLDPHGPVGPLLDPPSRDPETFPDGANVEFVVRRGERHVAMRVHERGSGETRSCGTGACAVMVAAAVADGARPSGAD
VAYRVDVPGGTLTVTWTADDRVLLTGPAVIVARGTTVL

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=280, Percent_Identity=37.1428571428571, Blast_Score=130, Evalue=7e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 29272; Mature: 29141

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTPAPTAADGYPFLKGHGTENDFVLLPDLDGSLHGDLSADRVRALCDRHAGIGGDGVL
CCCCCCCCCCCCCCEEECCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCHHEE
RVLRTDAGWFMDYRNADGSIAEMCGNGIRVFVRHLVEEGLADPGQPIEVDTRDGVKVLTV
EEEECCCCEEEEEECCCCHHHHHHCCHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEEE
DGDLITADMGRPRVLGETVVGVGTVSWPARHVDMGNPHAVAFVDSLDPHGPVGPLLDPPS
CCCEEEECCCCCEEECCCEEEEECCCCCHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCC
RDPETFPDGANVEFVVRRGERHVAMRVHERGSGETRSCGTGACAVMVAAAVADGARPSGA
CCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
DVAYRVDVPGGTLTVTWTADDRVLLTGPAVIVARGTTVL
CEEEEEECCCCEEEEEEECCCEEEEECCEEEEECCCCCC
>Mature Secondary Structure 
TTTPAPTAADGYPFLKGHGTENDFVLLPDLDGSLHGDLSADRVRALCDRHAGIGGDGVL
CCCCCCCCCCCCCEEECCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCHHEE
RVLRTDAGWFMDYRNADGSIAEMCGNGIRVFVRHLVEEGLADPGQPIEVDTRDGVKVLTV
EEEECCCCEEEEEECCCCHHHHHHCCHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEEE
DGDLITADMGRPRVLGETVVGVGTVSWPARHVDMGNPHAVAFVDSLDPHGPVGPLLDPPS
CCCEEEECCCCCEEECCCEEEEECCCCCHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCC
RDPETFPDGANVEFVVRRGERHVAMRVHERGSGETRSCGTGACAVMVAAAVADGARPSGA
CCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
DVAYRVDVPGGTLTVTWTADDRVLLTGPAVIVARGTTVL
CEEEEEECCCCEEEEEEECCCEEEEECCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]