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The map label for this gene is rumB [H]

Identifier: 119717975

GI number: 119717975

Start: 3971620

End: 3972759

Strand: Reverse

Name: rumB [H]

Synonym: Noca_3753

Alternate gene names: 119717975

Gene position: 3972759-3971620 (Counterclockwise)

Preceding gene: 119717978

Following gene: 119717974

Centisome position: 79.68

GC content: 74.12

Gene sequence:

>1140_bases
ATGCTCTGCTCCCACTTCGACGCCGGACGCTGCCGGTCCTGCACCTGGCTCGGCACGGCGTACGCCGCGCAGCTGGGCGC
CAAGCAGCACACCGTCGAGGAGCTGCTCGCCCGGTCGGTCGGCGCCGCCGGGGCGACGGCCTGGCTGCCGCCGGTCGCCA
GCGCCCAGCTGGGCGTGCGCAACAAGGCGAAGATGGTGGTCAGCGGCACCCTCGCGGAGCCGACGCTCGGCATCCTGAAC
CCGGCCGGCGCCGGGGTGGACCTGCGCGACTGCCCGCTGCACACGCCGGGCATCCAGGCAGCGCTCCCGGTGCTCGCCGA
CTTCGTGACCCGGGCCGCGCTGGCGCCGTACGACCTGGCCACTCGCAGGGGTGAGCTGAAGTACCTGCTGGTCACCGAGT
CACCGGACGGTGAGCTGATGGTGCGGTTCGTGCTGCGCTCCCAGGAGCCGGTGGCGCGGATCCGCAAGCACCTGCCCGCG
CTGCTCGAGCGGCTGCCGCAGGTCGCGGTCGCGACCGTGAACCTCCAGCCCGAGCACAAGGCGGTCCTGGAGGGCGAGCG
CGAGATCGTGCTCACCGAGCAGGAGTCGCTGCTGATGCAGGTCGGCGGCCTCGGGCTGCACCTGCGGCCGCAGAGCTTCT
TCCAGACCAACACCGCGATGGCCGCCGAGCTCTACCGGCTCGCCCGCGCGTGGACGGCCGAGCTGGCCCCGGGCCGGGTC
TGGGACCTGTACTGCGGGGTGGGCGGCTTCGCCCTCGCGATCGCGTCCACGGCGCACGCGGAGGTCACCGGCGTCGAGGT
CAGCGGCGAGGCGATCGCCTCGGCGACCGCCAGCGCGCGGGAGGCGGGGCTCGACCGCGTGCGGTTCGAGGCCGGCGACG
CCACCGCCTGGGTGTCGGACCGGCCCGACGTCCCGGACCTGGTCGTCGTGAACCCGCCGCGACGGGGCCTCGGCCCCGAG
CTCAGCGGCTGGCTCGAGGACTCGGGCGTGCGGCACGTCCTGTACTCCAGCTGCAACGCCCACACGCTGGCCCGCGACCT
GGACGCGATGCCCTCGCTGCGCCCGGTGCGCGCGCAGCTGCTCGACATGTTCCCGAACACCGCGCACTACGAGGTCCTCG
CGCTGCTGGAGCGGTTGTGA

Upstream 100 bases:

>100_bases
GAGGCTAGCGTCGGGGCAGCGTCCGGTCCCGGGAACGGCCGTCCTGGTGCCACCGGATCAGACCAGTGCCGCACCGGGGC
CCCCGTAGCCTGGTGCGGTC

Downstream 100 bases:

>100_bases
CCGGCCCACGCGGGCGGCTCGACGAGGAGCGTCGCCGTACGACGGCCCGGCTGGCGGAACTCCGCGGGGAGTACCGCGGG
TTCGTCGACGCCTCGCGCGA

Product: 23S rRNA methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U747)-methyltransferase [H]

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MLCSHFDAGRCRSCTWLGTAYAAQLGAKQHTVEELLARSVGAAGATAWLPPVASAQLGVRNKAKMVVSGTLAEPTLGILN
PAGAGVDLRDCPLHTPGIQAALPVLADFVTRAALAPYDLATRRGELKYLLVTESPDGELMVRFVLRSQEPVARIRKHLPA
LLERLPQVAVATVNLQPEHKAVLEGEREIVLTEQESLLMQVGGLGLHLRPQSFFQTNTAMAAELYRLARAWTAELAPGRV
WDLYCGVGGFALAIASTAHAEVTGVEVSGEAIASATASAREAGLDRVRFEAGDATAWVSDRPDVPDLVVVNPPRRGLGPE
LSGWLEDSGVRHVLYSSCNAHTLARDLDAMPSLRPVRAQLLDMFPNTAHYEVLALLERL

Sequences:

>Translated_379_residues
MLCSHFDAGRCRSCTWLGTAYAAQLGAKQHTVEELLARSVGAAGATAWLPPVASAQLGVRNKAKMVVSGTLAEPTLGILN
PAGAGVDLRDCPLHTPGIQAALPVLADFVTRAALAPYDLATRRGELKYLLVTESPDGELMVRFVLRSQEPVARIRKHLPA
LLERLPQVAVATVNLQPEHKAVLEGEREIVLTEQESLLMQVGGLGLHLRPQSFFQTNTAMAAELYRLARAWTAELAPGRV
WDLYCGVGGFALAIASTAHAEVTGVEVSGEAIASATASAREAGLDRVRFEAGDATAWVSDRPDVPDLVVVNPPRRGLGPE
LSGWLEDSGVRHVLYSSCNAHTLARDLDAMPSLRPVRAQLLDMFPNTAHYEVLALLERL
>Mature_379_residues
MLCSHFDAGRCRSCTWLGTAYAAQLGAKQHTVEELLARSVGAAGATAWLPPVASAQLGVRNKAKMVVSGTLAEPTLGILN
PAGAGVDLRDCPLHTPGIQAALPVLADFVTRAALAPYDLATRRGELKYLLVTESPDGELMVRFVLRSQEPVARIRKHLPA
LLERLPQVAVATVNLQPEHKAVLEGEREIVLTEQESLLMQVGGLGLHLRPQSFFQTNTAMAAELYRLARAWTAELAPGRV
WDLYCGVGGFALAIASTAHAEVTGVEVSGEAIASATASAREAGLDRVRFEAGDATAWVSDRPDVPDLVVVNPPRRGLGPE
LSGWLEDSGVRHVLYSSCNAHTLARDLDAMPSLRPVRAQLLDMFPNTAHYEVLALLERL

Specific function: Catalyzes the formation of 5-methyl-uridine at position 747 (M-5-U747) in 23S rRNA [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. RumB subfamily [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=423, Percent_Identity=29.3144208037825, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI51173878, Length=423, Percent_Identity=29.3144208037825, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI1787083, Length=377, Percent_Identity=50.132625994695, Blast_Score=372, Evalue=1e-104,
Organism=Escherichia coli, GI1789148, Length=385, Percent_Identity=26.7532467532467, Blast_Score=100, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24666579, Length=306, Percent_Identity=27.4509803921569, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011825
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.189 [H]

Molecular weight: Translated: 40690; Mature: 40690

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS01230 TRMA_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLCSHFDAGRCRSCTWLGTAYAAQLGAKQHTVEELLARSVGAAGATAWLPPVASAQLGVR
CCCCCCCCCCCCCEEEHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCC
NKAKMVVSGTLAEPTLGILNPAGAGVDLRDCPLHTPGIQAALPVLADFVTRAALAPYDLA
CCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHH
TRRGELKYLLVTESPDGELMVRFVLRSQEPVARIRKHLPALLERLPQVAVATVNLQPEHK
HCCCCEEEEEEEECCCCCEEHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCHH
AVLEGEREIVLTEQESLLMQVGGLGLHLRPQSFFQTNTAMAAELYRLARAWTAELAPGRV
HHHCCCCEEEEECHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
WDLYCGVGGFALAIASTAHAEVTGVEVSGEAIASATASAREAGLDRVRFEAGDATAWVSD
EEEHHCCCHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHCCHHEEEECCCCEEEECC
RPDVPDLVVVNPPRRGLGPELSGWLEDSGVRHVLYSSCNAHTLARDLDAMPSLRPVRAQL
CCCCCCEEEECCCCCCCCCCCHHHHHCCCCHHHHHHCCCHHHHHHHHHHCCCCHHHHHHH
LDMFPNTAHYEVLALLERL
HHHCCCCHHHHHHHHHHHC
>Mature Secondary Structure
MLCSHFDAGRCRSCTWLGTAYAAQLGAKQHTVEELLARSVGAAGATAWLPPVASAQLGVR
CCCCCCCCCCCCCEEEHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCC
NKAKMVVSGTLAEPTLGILNPAGAGVDLRDCPLHTPGIQAALPVLADFVTRAALAPYDLA
CCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHH
TRRGELKYLLVTESPDGELMVRFVLRSQEPVARIRKHLPALLERLPQVAVATVNLQPEHK
HCCCCEEEEEEEECCCCCEEHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCHH
AVLEGEREIVLTEQESLLMQVGGLGLHLRPQSFFQTNTAMAAELYRLARAWTAELAPGRV
HHHCCCCEEEEECHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
WDLYCGVGGFALAIASTAHAEVTGVEVSGEAIASATASAREAGLDRVRFEAGDATAWVSD
EEEHHCCCHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHCCHHEEEECCCCEEEECC
RPDVPDLVVVNPPRRGLGPELSGWLEDSGVRHVLYSSCNAHTLARDLDAMPSLRPVRAQL
CCCCCCEEEECCCCCCCCCCCHHHHHCCCCHHHHHHCCCHHHHHHHHHHCCCCHHHHHHH
LDMFPNTAHYEVLALLERL
HHHCCCCHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]