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The map label for this gene is pcrA [H]

Identifier: 119717815

GI number: 119717815

Start: 3811375

End: 3813768

Strand: Reverse

Name: pcrA [H]

Synonym: Noca_3593

Alternate gene names: 119717815

Gene position: 3813768-3811375 (Counterclockwise)

Preceding gene: 119717816

Following gene: 119717813

Centisome position: 76.49

GC content: 71.6

Gene sequence:

>2394_bases
ATGCCCGCCACCCCCACGTCCGACGGGCCGGAGCCGACCCGCCGCGGACCCACTCGCGAGGAGCTGCTCGAGGGGCTCAA
CGAGCCCCAGCGGGCGGCCGTCGTCCACGAGGGCGCGCCGCTCCTGGTCGTCGCGGGAGCGGGCTCGGGCAAGACCCGGG
TGCTGACCCGCCGGATCGCCTGGCTGATCTCCGAGCGGAAGGCCCACCCCGGGTCGATCCTGGCGATCACGTTCACCAAC
AAGGCCGCGGCCGAGATGAAGGAGCGGGTCGAGGACCTGGTCGGCCGGCGGGCCCGGATCATGTGGGTCTCGACGTTCCA
CTCCGCCTGCGTGCGGATCCTCCGCAAGGAAGTCGACAAGCTGGGGTTCAAGTCGAACTTCTCGATCTACGACGCGGCCG
ACCAGAAGCGGCTGATGACGCTGGTCTGCAACGACCTGGATCTCGACCCGAAGCGCTACCAGCCGGGCGCGCTGCTGCAC
TGGGTGTCCAACCAGAAGAACGAGCTGCGCGACCCCGACGAGGCGACCAAGGACGCGCGCAACCACCTGGAGGAGACCTA
CGCCGCGGCGTACACCACCTACCAGCGGCGGCTGCGCCAGGCCAACGCGCTCGACTTCGACGACCTGATCATGTTCACGG
TCCACCTGTTCCAGCAGTTCCCCGAGGTGCGCGAGACCTACCGGCGCCGGTTCCGGCACGTGCTCGTCGACGAGTACCAG
GACACCAACCACGCGCAGTACGCCTTCATCCACCAGCTCTGCGCCGACCGGATGGAGGAGCTGGACCCGTCCGCGGAGCG
CATCGAGCCGGCCGAGCTGATGGTCGTCGGTGACGCCGACCAGTCGATCTACGCGTTCCGGGGCGCCAACATCCGCAACA
TCCTCGACTTCGAGCAGGACTTCCCGAACGCCACCTCGATCCTGCTCGAGCAGAACTACCGCTCCACGCAGACGATCCTG
ACCGCCGCGAACTCGGTGATCGGCCACAACCAGGCGCGCAAGCCCAAGCGGCTGTGGTCCGACGCCGGCGACGGCGAGCG
GATCGTCGGGTACGTCGCCGACGACGAGCACGACGAGGCGCGGTTCGTCTCCGAGGAGATCGACCGGCTCACCGACGCGA
AGGCCTACCGGCCCGGGGACGTCGCCGTGTTCTACCGCACGAACGCCCAGTCGCGAGTGTTCGAGGAGATCTTCATCCGC
CTCGGCATGCCCTACAAGGTGGTCGGGGGCGTCCGCTTCTACGAGCGCCGTGAGGTCCGGGACGCGCTCGCCTACCTTCG
GCTGCTGGTCAACCCTGCCGACCAGGTCTCGCTGCGCCGGATCCTGAACACCCCCAAGCGGGGCATCGGCGACCGGGCCG
TCGCGTGCGTCAACGCCCTCGCGGAGCGGGACCGGACCACGTTCTGGGAGGCGCTGCGCCGCGCGGAGGACGCGCCGGGG
CTCGCCACCCGGTCGCTGACCCAGATCCAAGGGTTCGTCGCGATGATCGAGGAGCTCCAGTCGATGGTCGACGGGGGCGA
GCGCCCCGACGTGATCCTGGAGACCACGCTGGCGCGCTCGGGCTACCTCACCGCGCTGGAGGAGAGCGACGACCCCCAGG
ACGAGACCCGGCTGGAGAACCTCGCCGAGCTGGTCGCCGTCGCGCGCGAGTTCGCCGACGACCCCGTCGCAGGTCCGTCC
GCCGACCCGGCCGACGTCGACGCCGGCACCGTCGCGCCGGGCCTGGCGGACTTCCTGGAGCGGGTCGCGCTGGTCGCCGA
CGCCGACCAGATCCCCGACGACGACCCCGAGGACGCCGGTGTCGTCACCCTGATGACCCTGCACACCGCGAAGGGCCTGG
AGTTCCCCGTCGTCTTCCTCACCGGTCTCGAGGACGGCGTGTTCCCGCACGCGCGCTCGCTCGGTGAGCAGCAGGAGCTC
GAGGAGGAGCGCCGGCTGGCGTACGTCGGCGTCACGCGGGCCCGCGAGCGCCTCTACGTCTCCCGGGCCGTGGTCCGCTC
GGCGTGGGGCGCGCCCTCGCACAACCCCGGGTCGAGGTTCCTCGACGAGCTGCCCGTCGACCTGGTCGACTGGCGCCGCA
CCGAGGCCGCCCAGACCCGCTGGGGGCGCCCCGACCTGGCGAGCGGCTCGGCCGCCCGGCTCGGCTCGCCCACCGCGGCC
GGCCGCCGGAACTTCTCCGCCGCCGCCGTCCGCGCCGACGCGGCGGCCCGGGCCAAGCCGGCCCGCGAGATCCCGAGCCT
CGAGCCCGGCGACCGGGTGCTGCACGACTCCTTCGGCATGGGCACGGTCGTCGCGCTCGAGGGGGTCGCGGAGAAGGCGG
TCGCCTCGATCGACTTCGGCTCCGAGGGCGTCAAGCGGCTGCTGTTGCGCTACGCGCCCGTGGAGAAGCTGTAG

Upstream 100 bases:

>100_bases
GCAGTGGCACGCTCTCGGTGGTGACGGTCGTCGTCTGAGACCGGGGGTCCCGACACGTAGGCTGGCGGACAGCATGAGCA
TCTCGATCCCCCTGCCCGGC

Downstream 100 bases:

>100_bases
CGCTCGTCCGCGCTACGGCGTCAGGCCGTGGACCACGAGCGCCTGGTACGGGTCGACGGGGTCGCCGGCGCCGGGGCGGA
CCTCGAGGTGCAGGTGCGGG

Product: ATP-dependent DNA helicase PcrA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 797; Mature: 796

Protein sequence:

>797_residues
MPATPTSDGPEPTRRGPTREELLEGLNEPQRAAVVHEGAPLLVVAGAGSGKTRVLTRRIAWLISERKAHPGSILAITFTN
KAAAEMKERVEDLVGRRARIMWVSTFHSACVRILRKEVDKLGFKSNFSIYDAADQKRLMTLVCNDLDLDPKRYQPGALLH
WVSNQKNELRDPDEATKDARNHLEETYAAAYTTYQRRLRQANALDFDDLIMFTVHLFQQFPEVRETYRRRFRHVLVDEYQ
DTNHAQYAFIHQLCADRMEELDPSAERIEPAELMVVGDADQSIYAFRGANIRNILDFEQDFPNATSILLEQNYRSTQTIL
TAANSVIGHNQARKPKRLWSDAGDGERIVGYVADDEHDEARFVSEEIDRLTDAKAYRPGDVAVFYRTNAQSRVFEEIFIR
LGMPYKVVGGVRFYERREVRDALAYLRLLVNPADQVSLRRILNTPKRGIGDRAVACVNALAERDRTTFWEALRRAEDAPG
LATRSLTQIQGFVAMIEELQSMVDGGERPDVILETTLARSGYLTALEESDDPQDETRLENLAELVAVAREFADDPVAGPS
ADPADVDAGTVAPGLADFLERVALVADADQIPDDDPEDAGVVTLMTLHTAKGLEFPVVFLTGLEDGVFPHARSLGEQQEL
EEERRLAYVGVTRARERLYVSRAVVRSAWGAPSHNPGSRFLDELPVDLVDWRRTEAAQTRWGRPDLASGSAARLGSPTAA
GRRNFSAAAVRADAAARAKPAREIPSLEPGDRVLHDSFGMGTVVALEGVAEKAVASIDFGSEGVKRLLLRYAPVEKL

Sequences:

>Translated_797_residues
MPATPTSDGPEPTRRGPTREELLEGLNEPQRAAVVHEGAPLLVVAGAGSGKTRVLTRRIAWLISERKAHPGSILAITFTN
KAAAEMKERVEDLVGRRARIMWVSTFHSACVRILRKEVDKLGFKSNFSIYDAADQKRLMTLVCNDLDLDPKRYQPGALLH
WVSNQKNELRDPDEATKDARNHLEETYAAAYTTYQRRLRQANALDFDDLIMFTVHLFQQFPEVRETYRRRFRHVLVDEYQ
DTNHAQYAFIHQLCADRMEELDPSAERIEPAELMVVGDADQSIYAFRGANIRNILDFEQDFPNATSILLEQNYRSTQTIL
TAANSVIGHNQARKPKRLWSDAGDGERIVGYVADDEHDEARFVSEEIDRLTDAKAYRPGDVAVFYRTNAQSRVFEEIFIR
LGMPYKVVGGVRFYERREVRDALAYLRLLVNPADQVSLRRILNTPKRGIGDRAVACVNALAERDRTTFWEALRRAEDAPG
LATRSLTQIQGFVAMIEELQSMVDGGERPDVILETTLARSGYLTALEESDDPQDETRLENLAELVAVAREFADDPVAGPS
ADPADVDAGTVAPGLADFLERVALVADADQIPDDDPEDAGVVTLMTLHTAKGLEFPVVFLTGLEDGVFPHARSLGEQQEL
EEERRLAYVGVTRARERLYVSRAVVRSAWGAPSHNPGSRFLDELPVDLVDWRRTEAAQTRWGRPDLASGSAARLGSPTAA
GRRNFSAAAVRADAAARAKPAREIPSLEPGDRVLHDSFGMGTVVALEGVAEKAVASIDFGSEGVKRLLLRYAPVEKL
>Mature_796_residues
PATPTSDGPEPTRRGPTREELLEGLNEPQRAAVVHEGAPLLVVAGAGSGKTRVLTRRIAWLISERKAHPGSILAITFTNK
AAAEMKERVEDLVGRRARIMWVSTFHSACVRILRKEVDKLGFKSNFSIYDAADQKRLMTLVCNDLDLDPKRYQPGALLHW
VSNQKNELRDPDEATKDARNHLEETYAAAYTTYQRRLRQANALDFDDLIMFTVHLFQQFPEVRETYRRRFRHVLVDEYQD
TNHAQYAFIHQLCADRMEELDPSAERIEPAELMVVGDADQSIYAFRGANIRNILDFEQDFPNATSILLEQNYRSTQTILT
AANSVIGHNQARKPKRLWSDAGDGERIVGYVADDEHDEARFVSEEIDRLTDAKAYRPGDVAVFYRTNAQSRVFEEIFIRL
GMPYKVVGGVRFYERREVRDALAYLRLLVNPADQVSLRRILNTPKRGIGDRAVACVNALAERDRTTFWEALRRAEDAPGL
ATRSLTQIQGFVAMIEELQSMVDGGERPDVILETTLARSGYLTALEESDDPQDETRLENLAELVAVAREFADDPVAGPSA
DPADVDAGTVAPGLADFLERVALVADADQIPDDDPEDAGVVTLMTLHTAKGLEFPVVFLTGLEDGVFPHARSLGEQQELE
EERRLAYVGVTRARERLYVSRAVVRSAWGAPSHNPGSRFLDELPVDLVDWRRTEAAQTRWGRPDLASGSAARLGSPTAAG
RRNFSAAAVRADAAARAKPAREIPSLEPGDRVLHDSFGMGTVVALEGVAEKAVASIDFGSEGVKRLLLRYAPVEKL

Specific function: Has Both Atpase And Helicase Activities. Unwinds DNA Duplexes With 3' To 5' Polarity With Respect To The Bound Strand And Initiates Unwinding Most Effectively When A Single-Stranded Region Is Present. Involved In The Post-Incision Events Of Nucleotide Exc

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=780, Percent_Identity=40.3846153846154, Blast_Score=520, Evalue=1e-148,
Organism=Escherichia coli, GI48994965, Length=703, Percent_Identity=37.2688477951636, Blast_Score=385, Evalue=1e-108,
Organism=Escherichia coli, GI1787196, Length=354, Percent_Identity=27.1186440677966, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6322369, Length=761, Percent_Identity=28.515111695138, Blast_Score=227, Evalue=7e-60,
Organism=Saccharomyces cerevisiae, GI6324477, Length=690, Percent_Identity=22.8985507246377, Blast_Score=116, Evalue=1e-26,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 88794; Mature: 88663

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPATPTSDGPEPTRRGPTREELLEGLNEPQRAAVVHEGAPLLVVAGAGSGKTRVLTRRIA
CCCCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHEEECCCCEEEEECCCCCCHHHHHHHHH
WLISERKAHPGSILAITFTNKAAAEMKERVEDLVGRRARIMWVSTFHSACVRILRKEVDK
HHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHH
LGFKSNFSIYDAADQKRLMTLVCNDLDLDPKRYQPGALLHWVSNQKNELRDPDEATKDAR
CCCCCCCCEEECCHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCHHCCCCHHHHHHHH
NHLEETYAAAYTTYQRRLRQANALDFDDLIMFTVHLFQQFPEVRETYRRRFRHVLVDEYQ
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DTNHAQYAFIHQLCADRMEELDPSAERIEPAELMVVGDADQSIYAFRGANIRNILDFEQD
CCCCHHHHHHHHHHHHHHHHCCCCHHHCCCEEEEEEECCCCCEEEECCCCHHHHHHHHHC
FPNATSILLEQNYRSTQTILTAANSVIGHNQARKPKRLWSDAGDGERIVGYVADDEHDEA
CCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCEEEEEECCCCCHHH
RFVSEEIDRLTDAKAYRPGDVAVFYRTNAQSRVFEEIFIRLGMPYKVVGGVRFYERREVR
HHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHH
DALAYLRLLVNPADQVSLRRILNTPKRGIGDRAVACVNALAERDRTTFWEALRRAEDAPG
HHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCC
LATRSLTQIQGFVAMIEELQSMVDGGERPDVILETTLARSGYLTALEESDDPQDETRLEN
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHCCCCEEEECCCCCCHHHHHHHH
LAELVAVAREFADDPVAGPSADPADVDAGTVAPGLADFLERVALVADADQIPDDDPEDAG
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
VVTLMTLHTAKGLEFPVVFLTGLEDGVFPHARSLGEQQELEEERRLAYVGVTRARERLYV
EEEEEEEHHCCCCCCCEEEEECCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
SRAVVRSAWGAPSHNPGSRFLDELPVDLVDWRRTEAAQTRWGRPDLASGSAARLGSPTAA
HHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
GRRNFSAAAVRADAAARAKPAREIPSLEPGDRVLHDSFGMGTVVALEGVAEKAVASIDFG
CCCCCHHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCCC
SEGVKRLLLRYAPVEKL
HHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
PATPTSDGPEPTRRGPTREELLEGLNEPQRAAVVHEGAPLLVVAGAGSGKTRVLTRRIA
CCCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHEEECCCCEEEEECCCCCCHHHHHHHHH
WLISERKAHPGSILAITFTNKAAAEMKERVEDLVGRRARIMWVSTFHSACVRILRKEVDK
HHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHH
LGFKSNFSIYDAADQKRLMTLVCNDLDLDPKRYQPGALLHWVSNQKNELRDPDEATKDAR
CCCCCCCCEEECCHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCHHCCCCHHHHHHHH
NHLEETYAAAYTTYQRRLRQANALDFDDLIMFTVHLFQQFPEVRETYRRRFRHVLVDEYQ
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DTNHAQYAFIHQLCADRMEELDPSAERIEPAELMVVGDADQSIYAFRGANIRNILDFEQD
CCCCHHHHHHHHHHHHHHHHCCCCHHHCCCEEEEEEECCCCCEEEECCCCHHHHHHHHHC
FPNATSILLEQNYRSTQTILTAANSVIGHNQARKPKRLWSDAGDGERIVGYVADDEHDEA
CCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCEEEEEECCCCCHHH
RFVSEEIDRLTDAKAYRPGDVAVFYRTNAQSRVFEEIFIRLGMPYKVVGGVRFYERREVR
HHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHH
DALAYLRLLVNPADQVSLRRILNTPKRGIGDRAVACVNALAERDRTTFWEALRRAEDAPG
HHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCC
LATRSLTQIQGFVAMIEELQSMVDGGERPDVILETTLARSGYLTALEESDDPQDETRLEN
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHCCCCEEEECCCCCCHHHHHHHH
LAELVAVAREFADDPVAGPSADPADVDAGTVAPGLADFLERVALVADADQIPDDDPEDAG
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
VVTLMTLHTAKGLEFPVVFLTGLEDGVFPHARSLGEQQELEEERRLAYVGVTRARERLYV
EEEEEEEHHCCCCCCCEEEEECCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
SRAVVRSAWGAPSHNPGSRFLDELPVDLVDWRRTEAAQTRWGRPDLASGSAARLGSPTAA
HHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
GRRNFSAAAVRADAAARAKPAREIPSLEPGDRVLHDSFGMGTVVALEGVAEKAVASIDFG
CCCCCHHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCCC
SEGVKRLLLRYAPVEKL
HHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]