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The map label for this gene is nei [C]

Identifier: 119717704

GI number: 119717704

Start: 3692409

End: 3693257

Strand: Reverse

Name: nei [C]

Synonym: Noca_3482

Alternate gene names: 119717704

Gene position: 3693257-3692409 (Counterclockwise)

Preceding gene: 119717705

Following gene: 119717703

Centisome position: 74.07

GC content: 74.09

Gene sequence:

>849_bases
ATGCCTGAGGGCCACACCCTCCACCGCCTGGCCGACGAGATCACCGCGACCTTCGCCGGCCGGGTGGTGCGCACCGGCAG
CCCGCAGGGCCGGTTCGCCGACTCGGCCGCCCTCGTCGACGGGCAGGTGCTGGTCGGCGCCGAGGCCTGGGGCAAGCACC
TGTTCATCGCCTTCCCCGACGAGCGCTTCGTGCACATCCACCTCGGGCTCTACGGGAAGCTCGACCTGGTCGCGGGCGTC
GAGCAGGTGCCGGCGGCGGTCGGGCAGGTGCGCCTGCGGATCGTGGCCGCGGACCGGTCCTGGCACGCGTACGCCGACCT
GCGCGGCGCCACGACCTGCGAGCTGGTGACCCGGGAGCAGCGTGACGCCGTCGTCGCGCGCTCGGGTCCCGACCCGCTGC
GTGCCGATGCCGATCCAGGCCGGGCCTGGGCCCGGATCCGCCGCAGCAAGGCGCCGATCGGCGGCCTGCTGATGGACCAG
GCCGTGCTTGCCGGCGTCGGGAACGTCTACCGGGCCGAGCTCCTGTTCCGGCACCGGATCGACCCGCAACGCCCCGGCAA
CACGCTGCGCGTCGGGCAGTGGCAGGCGATGTGGGACGACCTGGTGGTGCTGATGCGCGAGGGCGTCCGGACCGGCCGGA
TCGACACCGTGCGGCCCGAGCACACGCCCGAGGCGATGGGCCGGCCGCCGCGCCGCGACGACCACGGGGGCGAGGTCTAC
GTCTACCGCCGGACCGGACAGCCGTGCCACGTCTGCGGCGCCACGGTCCGCACGGCCGAGCTCCAGGGGAGAAACCTGTT
CTGGTGTCCGCGTTGCCAGCCCCGGTTCCGATCCCGGGCATTACAGTGA

Upstream 100 bases:

>100_bases
TTCCTCGCCGAGCCGTTCAGCGGCGAGGAGCGACACGTGCGTCGGATCGGGCAGCTGGCGGCGTACGAGAGCACCGGCGA
GCTCCCCGCCCTCCCCGGCG

Downstream 100 bases:

>100_bases
GCACCCCCGAGCACGAGGACCGATCCCGACCATGACCGCTCCCTCCGAGGTGGCCCGGCCCCTCCGGTCCGCGCGGCGGC
GACCCTCCGTCAGCCAGGCC

Product: formamidopyrimidine-DNA glycolase

Products: NA

Alternate protein names: Putative DNA-(apurinic or apyrimidinic site) lyase Rv2464c/MT2539; Putative AP lyase Rv2464c/MT2539 [H]

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MPEGHTLHRLADEITATFAGRVVRTGSPQGRFADSAALVDGQVLVGAEAWGKHLFIAFPDERFVHIHLGLYGKLDLVAGV
EQVPAAVGQVRLRIVAADRSWHAYADLRGATTCELVTREQRDAVVARSGPDPLRADADPGRAWARIRRSKAPIGGLLMDQ
AVLAGVGNVYRAELLFRHRIDPQRPGNTLRVGQWQAMWDDLVVLMREGVRTGRIDTVRPEHTPEAMGRPPRRDDHGGEVY
VYRRTGQPCHVCGATVRTAELQGRNLFWCPRCQPRFRSRALQ

Sequences:

>Translated_282_residues
MPEGHTLHRLADEITATFAGRVVRTGSPQGRFADSAALVDGQVLVGAEAWGKHLFIAFPDERFVHIHLGLYGKLDLVAGV
EQVPAAVGQVRLRIVAADRSWHAYADLRGATTCELVTREQRDAVVARSGPDPLRADADPGRAWARIRRSKAPIGGLLMDQ
AVLAGVGNVYRAELLFRHRIDPQRPGNTLRVGQWQAMWDDLVVLMREGVRTGRIDTVRPEHTPEAMGRPPRRDDHGGEVY
VYRRTGQPCHVCGATVRTAELQGRNLFWCPRCQPRFRSRALQ
>Mature_281_residues
PEGHTLHRLADEITATFAGRVVRTGSPQGRFADSAALVDGQVLVGAEAWGKHLFIAFPDERFVHIHLGLYGKLDLVAGVE
QVPAAVGQVRLRIVAADRSWHAYADLRGATTCELVTREQRDAVVARSGPDPLRADADPGRAWARIRRSKAPIGGLLMDQA
VLAGVGNVYRAELLFRHRIDPQRPGNTLRVGQWQAMWDDLVVLMREGVRTGRIDTVRPEHTPEAMGRPPRRDDHGGEVYV
YRRTGQPCHVCGATVRTAELQGRNLFWCPRCQPRFRSRALQ

Specific function: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA b

COG id: COG0266

COG function: function code L; Formamidopyrimidine-DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FPG-type zinc finger [H]

Homologues:

Organism=Escherichia coli, GI1786932, Length=285, Percent_Identity=28.7719298245614, Blast_Score=100, Evalue=7e-23,
Organism=Escherichia coli, GI1790066, Length=263, Percent_Identity=24.3346007604563, Blast_Score=82, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015886
- InterPro:   IPR015887
- InterPro:   IPR012319
- InterPro:   IPR010979
- InterPro:   IPR000214
- InterPro:   IPR010663 [H]

Pfam domain/function: PF01149 Fapy_DNA_glyco; PF06831 H2TH; PF06827 zf-FPG_IleRS [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 31352; Mature: 31221

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: PS01242 ZF_FPG_1 ; PS51066 ZF_FPG_2 ; PS51068 FPG_CAT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEGHTLHRLADEITATFAGRVVRTGSPQGRFADSAALVDGQVLVGAEAWGKHLFIAFPD
CCCCHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCEEEECCEEEEECCCCCCEEEEEECC
ERFVHIHLGLYGKLDLVAGVEQVPAAVGQVRLRIVAADRSWHAYADLRGATTCELVTREQ
CCEEEEEEECCCCHHHHHCHHHHHHHHCEEEEEEEEECCCCEEEECCCCCCEEEEEHHCC
RDAVVARSGPDPLRADADPGRAWARIRRSKAPIGGLLMDQAVLAGVGNVYRAELLFRHRI
CCEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCC
DPQRPGNTLRVGQWQAMWDDLVVLMREGVRTGRIDTVRPEHTPEAMGRPPRRDDHGGEVY
CCCCCCCCEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCEEE
VYRRTGQPCHVCGATVRTAELQGRNLFWCPRCQPRFRSRALQ
EEECCCCCHHHHCCEEEEEEECCCCEEECCCCCHHHHHHCCC
>Mature Secondary Structure 
PEGHTLHRLADEITATFAGRVVRTGSPQGRFADSAALVDGQVLVGAEAWGKHLFIAFPD
CCCHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCEEEECCEEEEECCCCCCEEEEEECC
ERFVHIHLGLYGKLDLVAGVEQVPAAVGQVRLRIVAADRSWHAYADLRGATTCELVTREQ
CCEEEEEEECCCCHHHHHCHHHHHHHHCEEEEEEEEECCCCEEEECCCCCCEEEEEHHCC
RDAVVARSGPDPLRADADPGRAWARIRRSKAPIGGLLMDQAVLAGVGNVYRAELLFRHRI
CCEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCC
DPQRPGNTLRVGQWQAMWDDLVVLMREGVRTGRIDTVRPEHTPEAMGRPPRRDDHGGEVY
CCCCCCCCEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCEEE
VYRRTGQPCHVCGATVRTAELQGRNLFWCPRCQPRFRSRALQ
EEECCCCCHHHHCCEEEEEEECCCCEEECCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]