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The map label for this gene is proB [H]

Identifier: 119717670

GI number: 119717670

Start: 3655266

End: 3656381

Strand: Reverse

Name: proB [H]

Synonym: Noca_3448

Alternate gene names: 119717670

Gene position: 3656381-3655266 (Counterclockwise)

Preceding gene: 119717671

Following gene: 119717669

Centisome position: 73.33

GC content: 74.46

Gene sequence:

>1116_bases
ATGAGCCTGCGCCACGAGGTCACCGAGGCGAAGCGGATCGTCGTCAAGATCGGTTCCTCCTCGCTCACCACGGCGGCCGG
CGGCATCGACCCGGGCCGCGTGCGCGACCTCGTCGACGTGCTCGCGAAGTTGCGCGCCGGCGGCAGCGAGGTGGTGCTGG
TCTCCTCGGGCGCGATCGCTGCCGGACTGGCGCCGCTCGGGCTGCGGCGCCGGCCCCGCTCCCTCCCCGCCCAGCAGGCC
GCCGCCTCCGTCGGGCAGGGGCTGCTCGTGCACCGCTACAACGAGGAGCTCGCCCGGCACGGCGTGGTCGCCGGCCAGGT
GCTGCTCACCGTCGACGACGTCACCCGCCGCTCGCACTACCGCAACGCCTACCAGACCTTCGCCAAGCTGCTCGAGCTCG
GCGTGCTGCCGATCGTCAACGAGAACGACACCGTCGCGACCAGCGAGATCCGGTTCGGCGACAACGACCGGCTGGCGGCC
CTCGTCGCACACCTGGTGCACGCCGACCTGCTGGTGCTGCTCTCCGACGTCGAGGGCCTGTACGACGCCGACCCGGCCGC
GCCGGGCAGCTCGATGCTGCCCGACGTCGTCTCGGTCCACGACCTCGCCGACGTACGCATCGGCAAGGCCGGCGCCGCCG
GCCTCGGCACCGGTGGCATGCACACCAAGATCGACGCCGCCCGGATCGCGACCGGTGCCGGGATCCCCGTCGTGCTCACC
TCCGCCGAGCGCGCGCCGGCCGCGCTGCTCGGGGAGCCGGTCGGAACGCTGTTCCACGCCACCGGCCGACGGCGGCCGAC
CCGGTTGCTGTGGCTGGCCCACGCGACCGAGCCGAAGGGGACGCTGCTGCTGGACGCGGGCGCGGTCCGCGCCGTCGTCG
AGCGCCGGGCCTCGCTGCTGGCCGCCGGGATCACCGGGGTCGAGGGGACCTTCCACGCGGGCGACCCGGTGGACATCGCC
GCGCCGGACGGCACCCCGGTCGCGCGCGGCCTGGTCAACTTCGACGCCGAGGAGCTCCCGAACCTCCTCGGCCGCTCCTC
CCACGAGCTCAAGCGCGAGCTCGGGGCGGGCTACGAGCGCGAGGTCGTCCACCGCGACGACCTGGTCCTGCTGTAG

Upstream 100 bases:

>100_bases
CGGCGGAGCCTACGGGCCGCAGTCCTACGAGATCGGCGGCAGGGACGACCCGGACTGGGCCGAGGAGGATCTCGGGGAAT
GAGCAGGAGATGAGGCAGTC

Downstream 100 bases:

>100_bases
ACGGGGGTGGCGGTTTCCCCGGTTCACCGGGGAAGTTGGAACTTCTGGCCCGAAATTTTGGCCCAACAGTTCCGGTTTCC
CCGGTTCACCGGGGAAACCG

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MSLRHEVTEAKRIVVKIGSSSLTTAAGGIDPGRVRDLVDVLAKLRAGGSEVVLVSSGAIAAGLAPLGLRRRPRSLPAQQA
AASVGQGLLVHRYNEELARHGVVAGQVLLTVDDVTRRSHYRNAYQTFAKLLELGVLPIVNENDTVATSEIRFGDNDRLAA
LVAHLVHADLLVLLSDVEGLYDADPAAPGSSMLPDVVSVHDLADVRIGKAGAAGLGTGGMHTKIDAARIATGAGIPVVLT
SAERAPAALLGEPVGTLFHATGRRRPTRLLWLAHATEPKGTLLLDAGAVRAVVERRASLLAAGITGVEGTFHAGDPVDIA
APDGTPVARGLVNFDAEELPNLLGRSSHELKRELGAGYEREVVHRDDLVLL

Sequences:

>Translated_371_residues
MSLRHEVTEAKRIVVKIGSSSLTTAAGGIDPGRVRDLVDVLAKLRAGGSEVVLVSSGAIAAGLAPLGLRRRPRSLPAQQA
AASVGQGLLVHRYNEELARHGVVAGQVLLTVDDVTRRSHYRNAYQTFAKLLELGVLPIVNENDTVATSEIRFGDNDRLAA
LVAHLVHADLLVLLSDVEGLYDADPAAPGSSMLPDVVSVHDLADVRIGKAGAAGLGTGGMHTKIDAARIATGAGIPVVLT
SAERAPAALLGEPVGTLFHATGRRRPTRLLWLAHATEPKGTLLLDAGAVRAVVERRASLLAAGITGVEGTFHAGDPVDIA
APDGTPVARGLVNFDAEELPNLLGRSSHELKRELGAGYEREVVHRDDLVLL
>Mature_370_residues
SLRHEVTEAKRIVVKIGSSSLTTAAGGIDPGRVRDLVDVLAKLRAGGSEVVLVSSGAIAAGLAPLGLRRRPRSLPAQQAA
ASVGQGLLVHRYNEELARHGVVAGQVLLTVDDVTRRSHYRNAYQTFAKLLELGVLPIVNENDTVATSEIRFGDNDRLAAL
VAHLVHADLLVLLSDVEGLYDADPAAPGSSMLPDVVSVHDLADVRIGKAGAAGLGTGGMHTKIDAARIATGAGIPVVLTS
AERAPAALLGEPVGTLFHATGRRRPTRLLWLAHATEPKGTLLLDAGAVRAVVERRASLLAAGITGVEGTFHAGDPVDIAA
PDGTPVARGLVNFDAEELPNLLGRSSHELKRELGAGYEREVVHRDDLVLL

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=337, Percent_Identity=29.9703264094956, Blast_Score=115, Evalue=9e-26,
Organism=Homo sapiens, GI62912457, Length=335, Percent_Identity=30.1492537313433, Blast_Score=115, Evalue=1e-25,
Organism=Escherichia coli, GI1786437, Length=367, Percent_Identity=37.874659400545, Blast_Score=230, Evalue=1e-61,
Organism=Caenorhabditis elegans, GI17569967, Length=280, Percent_Identity=32.1428571428571, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17569969, Length=280, Percent_Identity=32.1428571428571, Blast_Score=127, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6320506, Length=422, Percent_Identity=33.6492890995261, Blast_Score=211, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6321822, Length=424, Percent_Identity=32.311320754717, Blast_Score=189, Evalue=7e-49,
Organism=Drosophila melanogaster, GI21357643, Length=290, Percent_Identity=33.1034482758621, Blast_Score=132, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 38812; Mature: 38681

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLRHEVTEAKRIVVKIGSSSLTTAAGGIDPGRVRDLVDVLAKLRAGGSEVVLVSSGAIA
CCCHHHHHHHHEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCHH
AGLAPLGLRRRPRSLPAQQAAASVGQGLLVHRYNEELARHGVVAGQVLLTVDDVTRRSHY
HCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEHHHHHHHHCCCEECEEEEEEHHHHHHHHH
RNAYQTFAKLLELGVLPIVNENDTVATSEIRFGDNDRLAALVAHLVHADLLVLLSDVEGL
HHHHHHHHHHHHHCCCEEECCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
YDADPAAPGSSMLPDVVSVHDLADVRIGKAGAAGLGTGGMHTKIDAARIATGAGIPVVLT
CCCCCCCCCCCCCCHHHHHHHHHHHEECCCCCCCCCCCCCCCCCHHHHHHCCCCCEEEEE
SAERAPAALLGEPVGTLFHATGRRRPTRLLWLAHATEPKGTLLLDAGAVRAVVERRASLL
CCCCCCHHHHCCCHHHHHHHCCCCCCEEEEEEEECCCCCCEEEEECHHHHHHHHHHHHHH
AAGITGVEGTFHAGDPVDIAAPDGTPVARGLVNFDAEELPNLLGRSSHELKRELGAGYER
HHCCCCCCCCCCCCCCCEEECCCCCHHHHHHHCCCHHHHHHHHCCCHHHHHHHHCCCCCH
EVVHRDDLVLL
HHCCCCCEEEC
>Mature Secondary Structure 
SLRHEVTEAKRIVVKIGSSSLTTAAGGIDPGRVRDLVDVLAKLRAGGSEVVLVSSGAIA
CCHHHHHHHHEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCHH
AGLAPLGLRRRPRSLPAQQAAASVGQGLLVHRYNEELARHGVVAGQVLLTVDDVTRRSHY
HCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEHHHHHHHHCCCEECEEEEEEHHHHHHHHH
RNAYQTFAKLLELGVLPIVNENDTVATSEIRFGDNDRLAALVAHLVHADLLVLLSDVEGL
HHHHHHHHHHHHHCCCEEECCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
YDADPAAPGSSMLPDVVSVHDLADVRIGKAGAAGLGTGGMHTKIDAARIATGAGIPVVLT
CCCCCCCCCCCCCCHHHHHHHHHHHEECCCCCCCCCCCCCCCCCHHHHHHCCCCCEEEEE
SAERAPAALLGEPVGTLFHATGRRRPTRLLWLAHATEPKGTLLLDAGAVRAVVERRASLL
CCCCCCHHHHCCCHHHHHHHCCCCCCEEEEEEEECCCCCCEEEEECHHHHHHHHHHHHHH
AAGITGVEGTFHAGDPVDIAAPDGTPVARGLVNFDAEELPNLLGRSSHELKRELGAGYER
HHCCCCCCCCCCCCCCCEEECCCCCHHHHHHHCCCHHHHHHHHCCCHHHHHHHHCCCCCH
EVVHRDDLVLL
HHCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572948; 12692562 [H]