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The map label for this gene is leuB [H]

Identifier: 119717591

GI number: 119717591

Start: 3568764

End: 3570200

Strand: Reverse

Name: leuB [H]

Synonym: Noca_3368

Alternate gene names: 119717591

Gene position: 3570200-3568764 (Counterclockwise)

Preceding gene: 119717593

Following gene: 119717590

Centisome position: 71.61

GC content: 70.63

Gene sequence:

>1437_bases
TTGCACGGTCTCTCGGTACATCTGAGTCGAAGATGTCTCCGCGCGAGGCCCGCCCCAGCAGTACGGCGTGATCCGCATCC
TGATGGTTCTCCCTCGGCTCGTGCGACCCACGAGCAGACCGCGCGTCCGGCGCCGGGCCATCTTCGTCTCAGGTGTATCG
GGACCGCACGGCTACCCATCCACCTGAAGAGCACACGAGAGAGAACAGACCGATGTACGACATGTACCCCGACTGGGGTC
CCGCCAGCCACCACCCCGACAACCCGCGCAGCTACGAGAACGTCACCGCGGCCGTCCAGGCCTCGCTCGACCTGCGGGAC
AACGGCGGCGAGCGCCCGGACGACAACTAGCCTCGGCCGCATGACCACCACCCACACTGGAACGTCCGGCTCCCTGCGCC
TCGCCGTCATCCCCGGCGACGGCATCGGGCCGGAGGTGACCGCCGAGGCCCTGAAGGTGCTCGAGGTCGCGTCCCCGGCC
GGCGTGAAGTTCGAGCAGACGCGGTACGACCTCGGCGCCGAGCGCTACCTCGCGACCGGCGAGGTGCTGCCCGACTCCGT
GCTCGAGGAGATCCGTGAGCACGACGCGATCCTGCTCGGCGCGGTCGGTGGCAAGCCGAACGACCCGAACCTGCCGCCCG
GCATCCTGGAGCGCGGGCTGCTGCTGCGGCTGCGCTTCGAGCTCGACCACTACGTCAACCTGCGGCCGTCCCGGATCTTC
CCGGGTGTCGCGTCGCCCCTCGCCAACCCTGGAGAGGTCGACTTCGTGGTGGTCCGCGAGGGCACCGAGGGCCCGTACAC
GGGCAACGGTGGCGCCCTGCGCGTCGGCACCCCGCACGAGATCGCCACCGAGGTCTCGGTGAACACCGCCTTCGGCGTCG
AGCGCGTCGTCCGCGACGCGTTCGCGCGCGCCCAGCGCCGGCCGCGCAAGAAGCTGACCCTGGTCCACAAGACCAACGTG
CTCGTCAACGCCGGTGCCGTGTGGTGGCGGATCACCCAGCAGGTGGCGGCGGAGTACCCCGAGGTCAGCGTCGACTACAT
GCACATCGACGCGGCGATGATCTTCATGACCACCGACCCCGCACGCTTCGACGTGATCGTCACCGACAACCTCTTCGGCG
ACATCATCACCGACCTCGCCGCCGCGATCACCGGCGGCATCGGCCTGGCGGCCTCCGGCAACGTCAACCCCGACCGGACC
GCGCCGTCCATGTTCGAGCCGGTGCACGGTTCGGCACCCGACATCGCGGGCCAGCAGAAGGCCGACCCCACCGCCGCGAT
CCTCTCCGCGTCCCTGCTGCTCGACCACCTCGGGTACGCCGACGCGGCCGCGGTCATCGAGACCGCGGCGATCGCCGATC
TCGCCGCGCGCGAGCCGGGCGCGGCCCGGCGTACCGCCGACGTCGGTGAGGCGATCGCCGCCCGAGTAGCCGGCTAG

Upstream 100 bases:

>100_bases
CCGTGCTCTACGGTGGTCGCACCATGCGGAGCGTCTTCGTACTTATTGAGCGCCGCGGCGAGGCTTCTTAAGAGAGGCCA
CCTCGCCGCGGAGTTCGGCG

Downstream 100 bases:

>100_bases
CCGGCGGGCTGCGGGATTCGGCTGTCGGCAGCCGAAACCCGCACGACACGCCGGCGTGCCTCGGCGTGTCGTGCGGGGAT
GGGCTGCCAGCAGCCGAACC

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MHGLSVHLSRRCLRARPAPAVRRDPHPDGSPSARATHEQTARPAPGHLRLRCIGTARLPIHLKSTRERTDRCTTCTPTGV
PPATTPTTRAATRTSPRPSRPRSTCGTTAASARTTTSLGRMTTTHTGTSGSLRLAVIPGDGIGPEVTAEALKVLEVASPA
GVKFEQTRYDLGAERYLATGEVLPDSVLEEIREHDAILLGAVGGKPNDPNLPPGILERGLLLRLRFELDHYVNLRPSRIF
PGVASPLANPGEVDFVVVREGTEGPYTGNGGALRVGTPHEIATEVSVNTAFGVERVVRDAFARAQRRPRKKLTLVHKTNV
LVNAGAVWWRITQQVAAEYPEVSVDYMHIDAAMIFMTTDPARFDVIVTDNLFGDIITDLAAAITGGIGLAASGNVNPDRT
APSMFEPVHGSAPDIAGQQKADPTAAILSASLLLDHLGYADAAAVIETAAIADLAAREPGAARRTADVGEAIAARVAG

Sequences:

>Translated_478_residues
MHGLSVHLSRRCLRARPAPAVRRDPHPDGSPSARATHEQTARPAPGHLRLRCIGTARLPIHLKSTRERTDRCTTCTPTGV
PPATTPTTRAATRTSPRPSRPRSTCGTTAASARTTTSLGRMTTTHTGTSGSLRLAVIPGDGIGPEVTAEALKVLEVASPA
GVKFEQTRYDLGAERYLATGEVLPDSVLEEIREHDAILLGAVGGKPNDPNLPPGILERGLLLRLRFELDHYVNLRPSRIF
PGVASPLANPGEVDFVVVREGTEGPYTGNGGALRVGTPHEIATEVSVNTAFGVERVVRDAFARAQRRPRKKLTLVHKTNV
LVNAGAVWWRITQQVAAEYPEVSVDYMHIDAAMIFMTTDPARFDVIVTDNLFGDIITDLAAAITGGIGLAASGNVNPDRT
APSMFEPVHGSAPDIAGQQKADPTAAILSASLLLDHLGYADAAAVIETAAIADLAAREPGAARRTADVGEAIAARVAG
>Mature_478_residues
MHGLSVHLSRRCLRARPAPAVRRDPHPDGSPSARATHEQTARPAPGHLRLRCIGTARLPIHLKSTRERTDRCTTCTPTGV
PPATTPTTRAATRTSPRPSRPRSTCGTTAASARTTTSLGRMTTTHTGTSGSLRLAVIPGDGIGPEVTAEALKVLEVASPA
GVKFEQTRYDLGAERYLATGEVLPDSVLEEIREHDAILLGAVGGKPNDPNLPPGILERGLLLRLRFELDHYVNLRPSRIF
PGVASPLANPGEVDFVVVREGTEGPYTGNGGALRVGTPHEIATEVSVNTAFGVERVVRDAFARAQRRPRKKLTLVHKTNV
LVNAGAVWWRITQQVAAEYPEVSVDYMHIDAAMIFMTTDPARFDVIVTDNLFGDIITDLAAAITGGIGLAASGNVNPDRT
APSMFEPVHGSAPDIAGQQKADPTAAILSASLLLDHLGYADAAAVIETAAIADLAAREPGAARRTADVGEAIAARVAG

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=354, Percent_Identity=32.7683615819209, Blast_Score=182, Evalue=9e-46,
Organism=Homo sapiens, GI28178838, Length=322, Percent_Identity=30.1242236024845, Blast_Score=142, Evalue=9e-34,
Organism=Homo sapiens, GI4758582, Length=322, Percent_Identity=30.1242236024845, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI28178816, Length=344, Percent_Identity=29.9418604651163, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI28178821, Length=324, Percent_Identity=30.8641975308642, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI28178819, Length=158, Percent_Identity=32.9113924050633, Blast_Score=89, Evalue=7e-18,
Organism=Escherichia coli, GI1788101, Length=316, Percent_Identity=47.4683544303797, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI87081683, Length=367, Percent_Identity=38.9645776566758, Blast_Score=209, Evalue=4e-55,
Organism=Escherichia coli, GI1787381, Length=380, Percent_Identity=30.7894736842105, Blast_Score=133, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71986051, Length=334, Percent_Identity=35.6287425149701, Blast_Score=174, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17550882, Length=359, Percent_Identity=30.6406685236769, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI25144293, Length=362, Percent_Identity=30.939226519337, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17505779, Length=341, Percent_Identity=29.6187683284457, Blast_Score=130, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6324709, Length=355, Percent_Identity=31.830985915493, Blast_Score=165, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6319830, Length=371, Percent_Identity=36.1185983827493, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6322097, Length=360, Percent_Identity=33.6111111111111, Blast_Score=149, Evalue=8e-37,
Organism=Saccharomyces cerevisiae, GI6324291, Length=365, Percent_Identity=30.1369863013699, Blast_Score=134, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24643270, Length=328, Percent_Identity=33.8414634146341, Blast_Score=179, Evalue=3e-45,
Organism=Drosophila melanogaster, GI24643268, Length=328, Percent_Identity=33.8414634146341, Blast_Score=179, Evalue=3e-45,
Organism=Drosophila melanogaster, GI24661184, Length=328, Percent_Identity=34.7560975609756, Blast_Score=163, Evalue=3e-40,
Organism=Drosophila melanogaster, GI161078635, Length=345, Percent_Identity=29.5652173913043, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI161078637, Length=345, Percent_Identity=29.5652173913043, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI161078633, Length=345, Percent_Identity=29.5652173913043, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24650122, Length=345, Percent_Identity=29.5652173913043, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI161078639, Length=343, Percent_Identity=29.7376093294461, Blast_Score=129, Evalue=6e-30,
Organism=Drosophila melanogaster, GI281362242, Length=344, Percent_Identity=31.1046511627907, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24648872, Length=344, Percent_Identity=31.1046511627907, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI20130355, Length=319, Percent_Identity=22.884012539185, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 50597; Mature: 50597

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHGLSVHLSRRCLRARPAPAVRRDPHPDGSPSARATHEQTARPAPGHLRLRCIGTARLPI
CCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCEEEEEEEEEECEE
HLKSTRERTDRCTTCTPTGVPPATTPTTRAATRTSPRPSRPRSTCGTTAASARTTTSLGR
EECCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCC
MTTTHTGTSGSLRLAVIPGDGIGPEVTAEALKVLEVASPAGVKFEQTRYDLGAERYLATG
EEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEHHHHHCCHHHEEECC
EVLPDSVLEEIREHDAILLGAVGGKPNDPNLPPGILERGLLLRLRFELDHYVNLRPSRIF
CCCCHHHHHHHHHCCEEEEECCCCCCCCCCCCHHHHCCCEEEEEEEECCCEECCCCCCCC
PGVASPLANPGEVDFVVVREGTEGPYTGNGGALRVGTPHEIATEVSVNTAFGVERVVRDA
CCHHHCCCCCCCEEEEEEECCCCCCCCCCCCEEEECCCHHHHHEEECHHHHHHHHHHHHH
FARAQRRPRKKLTLVHKTNVLVNAGAVWWRITQQVAAEYPEVSVDYMHIDAAMIFMTTDP
HHHHHHCCCHHEEEEEECCEEEECCHHHHHHHHHHHHCCCCCEEEEEEEEEEEEEEECCC
ARFDVIVTDNLFGDIITDLAAAITGGIGLAASGNVNPDRTAPSMFEPVHGSAPDIAGQQK
CEEEEEEECCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHCCCCCCCCCCCCCCC
ADPTAAILSASLLLDHLGYADAAAVIETAAIADLAAREPGAARRTADVGEAIAARVAG
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHGLSVHLSRRCLRARPAPAVRRDPHPDGSPSARATHEQTARPAPGHLRLRCIGTARLPI
CCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCEEEEEEEEEECEE
HLKSTRERTDRCTTCTPTGVPPATTPTTRAATRTSPRPSRPRSTCGTTAASARTTTSLGR
EECCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCC
MTTTHTGTSGSLRLAVIPGDGIGPEVTAEALKVLEVASPAGVKFEQTRYDLGAERYLATG
EEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEHHHHHCCHHHEEECC
EVLPDSVLEEIREHDAILLGAVGGKPNDPNLPPGILERGLLLRLRFELDHYVNLRPSRIF
CCCCHHHHHHHHHCCEEEEECCCCCCCCCCCCHHHHCCCEEEEEEEECCCEECCCCCCCC
PGVASPLANPGEVDFVVVREGTEGPYTGNGGALRVGTPHEIATEVSVNTAFGVERVVRDA
CCHHHCCCCCCCEEEEEEECCCCCCCCCCCCEEEECCCHHHHHEEECHHHHHHHHHHHHH
FARAQRRPRKKLTLVHKTNVLVNAGAVWWRITQQVAAEYPEVSVDYMHIDAAMIFMTTDP
HHHHHHCCCHHEEEEEECCEEEECCHHHHHHHHHHHHCCCCCEEEEEEEEEEEEEEECCC
ARFDVIVTDNLFGDIITDLAAAITGGIGLAASGNVNPDRTAPSMFEPVHGSAPDIAGQQK
CEEEEEEECCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHCCCCCCCCCCCCCCC
ADPTAAILSASLLLDHLGYADAAAVIETAAIADLAAREPGAARRTADVGEAIAARVAG
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]