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The map label for this gene is amt [H]

Identifier: 119717493

GI number: 119717493

Start: 3471844

End: 3473169

Strand: Reverse

Name: amt [H]

Synonym: Noca_3269

Alternate gene names: 119717493

Gene position: 3473169-3471844 (Counterclockwise)

Preceding gene: 119717494

Following gene: 119717492

Centisome position: 69.66

GC content: 67.87

Gene sequence:

>1326_bases
GTGATCCCTGGAGGTTCCGTGGACGGCTACTACGCCTTCATGCTCGTGGCGACAGCCCTCGTCCTGATGATGACCGTTCC
CGCACTGGCTCTGTTCTACGGCGGCATGAGCCGCTCGAAGTCCGTGCTGAACATGATGATGATGTCGTACGTCGCGATGG
CCGTGGTCGCCATCGTCTACGTGCTGTGGGGCTGGTCGATGACCTTCGGCCCGAACGACGTGGGTGGCCTGTTCGCCAAC
CCGTTCGACCTCTTCGGCCTCAACGACGTGACGCCCGACGGCTATCTCGCGGTGATGTTCCAGATGACGTTCGCCGCGAT
CACCGCGGCGCTCATCTCCGGCGCCATCGCGGACCGCGTCAAGCTCTCCTCCTGGGTCGTGTTCCTGCCCCTGTGGGTCA
CGCTCAGCTACTTCCCGATCGGGCACATGGTCTGGGGCGGCGGCTTCATCTCCGAGCACTTCGGGGCCCAGGACTACGCC
GGCGGCACGGTCGTCCACATCAACGCCGGCGTCGCCGGGCTCGTGCTCGCGCTGGTGATCGGCAAGCGGATCGGGTTCGG
CAAGGAGCCGATGCGCCCGCACAACCTGACCCTGACGATGATCGGGGCGGGCCTGCTGTGGTTCGGCTGGTACGGGTTCA
ACGTCGGCTCGATCGTCTTCACCGGGTCGACCGACGAGGAGAACATCGCGCAGTTCTACTCCGAGACCGGGGTGACGTTC
CTCAACACCACGATCGCGACCTGCGCCGCGATGCTCGGCTGGCTCCTCGTCGAGCGGCTCCTGCACGGCAAGGCCACCTC
GCTCGGTGCCGCGTCGGGCATCGTCGCCGGCCTGGTCGCCATCACGCCGTCGTGCGGCGCGGTGGAGCTGCCCGGCGCGA
TCCTGATCGGCGCGGTCGCCGGCGCCGCCTGCGCCTGGGCGGTGGGCCTGAAGTACAAGGTCGGCCTGGACGACTCGCTG
GACGTGGTGGGCGTCCACCTGGTCGGTGGCGTCATCGGGACGGTGCTGATCGGCCTGGTCTCGTCCTCGGACGCCCCGGG
CGGCATCGACGGCCTGTTCTACGGCGGCGGCTTCGGCTCTCTCGGCGACCAGGCGATGGCCGCCCTCATCGCGATCGTCT
GGTCGGCGGTCGCGACGACGATCATCGCGTACGCCATCAAGCTCACGATCGGCTGGCGGATCCCGGACGAGGCCGAGGTC
GAGGGGGTCGACTTCGACCAGCACGGTGAGACGGCGTACGACCTGCACAGTGGTCTCGGCGGCAGCGCGAACGCGCCCGC
CTCCGTCCTGGTCAAGAACACTGTCACCGAAGGGGTGAACGCATGA

Upstream 100 bases:

>100_bases
TGGCGCGGCGTACGTCGAAACCTCCCGACCACAGGGTTCTCACCAATGGGGCGGGGCCCGGTCGACATCGTCGTCGCCGC
CGGAGCCGCCCTCCCGAGAC

Downstream 100 bases:

>100_bases
GGCTCGTGACCGCGGTGATCAAGCCGCACAAGTGGGAGGACGTCCGTGAGGCGCTCGAGGCCTTCGGCGTGACCGGCATG
ACCGTCAGCGAGGTCAGCGG

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MIPGGSVDGYYAFMLVATALVLMMTVPALALFYGGMSRSKSVLNMMMMSYVAMAVVAIVYVLWGWSMTFGPNDVGGLFAN
PFDLFGLNDVTPDGYLAVMFQMTFAAITAALISGAIADRVKLSSWVVFLPLWVTLSYFPIGHMVWGGGFISEHFGAQDYA
GGTVVHINAGVAGLVLALVIGKRIGFGKEPMRPHNLTLTMIGAGLLWFGWYGFNVGSIVFTGSTDEENIAQFYSETGVTF
LNTTIATCAAMLGWLLVERLLHGKATSLGAASGIVAGLVAITPSCGAVELPGAILIGAVAGAACAWAVGLKYKVGLDDSL
DVVGVHLVGGVIGTVLIGLVSSSDAPGGIDGLFYGGGFGSLGDQAMAALIAIVWSAVATTIIAYAIKLTIGWRIPDEAEV
EGVDFDQHGETAYDLHSGLGGSANAPASVLVKNTVTEGVNA

Sequences:

>Translated_441_residues
MIPGGSVDGYYAFMLVATALVLMMTVPALALFYGGMSRSKSVLNMMMMSYVAMAVVAIVYVLWGWSMTFGPNDVGGLFAN
PFDLFGLNDVTPDGYLAVMFQMTFAAITAALISGAIADRVKLSSWVVFLPLWVTLSYFPIGHMVWGGGFISEHFGAQDYA
GGTVVHINAGVAGLVLALVIGKRIGFGKEPMRPHNLTLTMIGAGLLWFGWYGFNVGSIVFTGSTDEENIAQFYSETGVTF
LNTTIATCAAMLGWLLVERLLHGKATSLGAASGIVAGLVAITPSCGAVELPGAILIGAVAGAACAWAVGLKYKVGLDDSL
DVVGVHLVGGVIGTVLIGLVSSSDAPGGIDGLFYGGGFGSLGDQAMAALIAIVWSAVATTIIAYAIKLTIGWRIPDEAEV
EGVDFDQHGETAYDLHSGLGGSANAPASVLVKNTVTEGVNA
>Mature_441_residues
MIPGGSVDGYYAFMLVATALVLMMTVPALALFYGGMSRSKSVLNMMMMSYVAMAVVAIVYVLWGWSMTFGPNDVGGLFAN
PFDLFGLNDVTPDGYLAVMFQMTFAAITAALISGAIADRVKLSSWVVFLPLWVTLSYFPIGHMVWGGGFISEHFGAQDYA
GGTVVHINAGVAGLVLALVIGKRIGFGKEPMRPHNLTLTMIGAGLLWFGWYGFNVGSIVFTGSTDEENIAQFYSETGVTF
LNTTIATCAAMLGWLLVERLLHGKATSLGAASGIVAGLVAITPSCGAVELPGAILIGAVAGAACAWAVGLKYKVGLDDSL
DVVGVHLVGGVIGTVLIGLVSSSDAPGGIDGLFYGGGFGSLGDQAMAALIAIVWSAVATTIIAYAIKLTIGWRIPDEAEV
EGVDFDQHGETAYDLHSGLGGSANAPASVLVKNTVTEGVNA

Specific function: Involved in the uptake of ammonia (Probable) [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=415, Percent_Identity=44.578313253012, Blast_Score=322, Evalue=4e-89,
Organism=Caenorhabditis elegans, GI17550182, Length=443, Percent_Identity=27.9909706546275, Blast_Score=144, Evalue=8e-35,
Organism=Caenorhabditis elegans, GI17550180, Length=473, Percent_Identity=28.1183932346723, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI133931100, Length=430, Percent_Identity=26.046511627907, Blast_Score=115, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI32564042, Length=437, Percent_Identity=23.3409610983982, Blast_Score=101, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6325396, Length=423, Percent_Identity=33.806146572104, Blast_Score=211, Evalue=3e-55,
Organism=Saccharomyces cerevisiae, GI6321559, Length=419, Percent_Identity=33.890214797136, Blast_Score=209, Evalue=5e-55,
Organism=Saccharomyces cerevisiae, GI6324187, Length=422, Percent_Identity=35.3080568720379, Blast_Score=209, Evalue=7e-55,
Organism=Drosophila melanogaster, GI161078314, Length=442, Percent_Identity=26.6968325791855, Blast_Score=127, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 45692; Mature: 45692

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCHHHEEHHHHHCCCCC
>Mature Secondary Structure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CCCCCCHHHEEHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]