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The map label for this gene is trmD [H]

Identifier: 119717480

GI number: 119717480

Start: 3457126

End: 3458283

Strand: Reverse

Name: trmD [H]

Synonym: Noca_3256

Alternate gene names: 119717480

Gene position: 3458283-3457126 (Counterclockwise)

Preceding gene: 119717481

Following gene: 119717479

Centisome position: 69.36

GC content: 74.01

Gene sequence:

>1158_bases
GTGCGCATCGACATCGTCTCGATCTTCCCCGAGTACCTCGCGCCCCTGGAGCTCTCGCTGGCCGGGAAGGCGCGCGAGAA
GGGCCTGATCGACGTCCGGGTCCACGACCTGCGCGCCTGGACCCACGACCGGCACCGCACGGTCGACGACACCCCGTACG
GCGGCGGCGCCGGCATGGTGATGAAGCCCGAGCCGTGGGGCGAGGCCCTCGACGCGCTCGGCGTGCTCGACGGAGACGGC
GCGACCGTCGTGGTCACCACCCCGTCGGGGCAGCCGTTCACCCAGGAGGTCGCGCGCGAGCTCGCCACGCGGGAGCGGCT
GGTCTTCGCCTGCGGTCGCTACGAGGGGATCGACCAGCGGGTCCTGGAGGAGGTCGCCGAGCGCGCCGAGCTGCGCGAGA
TCTCACTGGGCGACTACGTGCTCAACGGAGGCGAGGTCGCCGCGCTCGCGATCACCGAGGCGGTCGTGCGGCTGGTGCCC
GGGTTCATGGGCAACGCCGAGTCGCTCCTCGAGGAGTCCCACGAGGGCGGCCTGCTGGAGTACCCCGTCTACACCAAGCC
CGGGTCCTGGCGCGGACGCGCCGTACCTCCCGTGCTGCTGTCGGGCGACCACGCCGCCATCGCGGCCTGGCGCCACGACC
AGGCCGTACGCCGGACCGCCGAGCGCCGGCCCGACCTCCTCCACCCCTCGGTGGTGCTGGACGGTGTCGAGGTGCGCGCG
GCCGAGCCGGCCGACGCGGGGGAGCTGTACGTGCTCCAGCTGGCCTGCTGGGTGCAGGAGCTGCACGCCAACCCGGGTGT
GCGGATCCCGGCGCTGCACGAGTCCCTGGAGGACCTGGCGGCCTGGCTGGACCGGGACACGGTGCTGGTGGCGCGGGCCG
GGGGTCGGCTGGTCGGGGCGGTGCGGGCGTCCCTGGACGAGCAGACCTGGGAGGTCGGCCGGCTGATGGTCGCGCCGGAC
CTCGCCGGTCGCGGGCTCGGCCGCACGCTGTTGACCCGCATCGAGGCCTTGGCGCCGCCCGAGGCCACGACGTACGCGCT
GTTCACGGGCGCGGGCAGCGCCCGCAACCAGCGGATGTACAAGAAGGCGGGCTACCGTCTGCGCGGCGAGGTCGAGCCGG
GGGTGGTCCGGATGACCAAGCGGATTTCCCGGCGCTGA

Upstream 100 bases:

>100_bases
TCAAGGCACTGGTCCCCGAGGTCGACCTCGCCGGCCGTCGCGTGGTCATCGCGGACCGTCCCGGCCTGGTCACGCCGCTG
CCCGAGGACGACGAGCACTA

Downstream 100 bases:

>100_bases
CGGAGCTGTGGCAGACTGGCTCCTCGGCCCAGTCGGCCAAACAAGGATCGCCCGGAGGACATGTCCGCCGGGGCTCCGCA
CCGGTTCCTGCCACAGGGGG

Product: tRNA (Guanine37-N1) methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MRIDIVSIFPEYLAPLELSLAGKAREKGLIDVRVHDLRAWTHDRHRTVDDTPYGGGAGMVMKPEPWGEALDALGVLDGDG
ATVVVTTPSGQPFTQEVARELATRERLVFACGRYEGIDQRVLEEVAERAELREISLGDYVLNGGEVAALAITEAVVRLVP
GFMGNAESLLEESHEGGLLEYPVYTKPGSWRGRAVPPVLLSGDHAAIAAWRHDQAVRRTAERRPDLLHPSVVLDGVEVRA
AEPADAGELYVLQLACWVQELHANPGVRIPALHESLEDLAAWLDRDTVLVARAGGRLVGAVRASLDEQTWEVGRLMVAPD
LAGRGLGRTLLTRIEALAPPEATTYALFTGAGSARNQRMYKKAGYRLRGEVEPGVVRMTKRISRR

Sequences:

>Translated_385_residues
MRIDIVSIFPEYLAPLELSLAGKAREKGLIDVRVHDLRAWTHDRHRTVDDTPYGGGAGMVMKPEPWGEALDALGVLDGDG
ATVVVTTPSGQPFTQEVARELATRERLVFACGRYEGIDQRVLEEVAERAELREISLGDYVLNGGEVAALAITEAVVRLVP
GFMGNAESLLEESHEGGLLEYPVYTKPGSWRGRAVPPVLLSGDHAAIAAWRHDQAVRRTAERRPDLLHPSVVLDGVEVRA
AEPADAGELYVLQLACWVQELHANPGVRIPALHESLEDLAAWLDRDTVLVARAGGRLVGAVRASLDEQTWEVGRLMVAPD
LAGRGLGRTLLTRIEALAPPEATTYALFTGAGSARNQRMYKKAGYRLRGEVEPGVVRMTKRISRR
>Mature_385_residues
MRIDIVSIFPEYLAPLELSLAGKAREKGLIDVRVHDLRAWTHDRHRTVDDTPYGGGAGMVMKPEPWGEALDALGVLDGDG
ATVVVTTPSGQPFTQEVARELATRERLVFACGRYEGIDQRVLEEVAERAELREISLGDYVLNGGEVAALAITEAVVRLVP
GFMGNAESLLEESHEGGLLEYPVYTKPGSWRGRAVPPVLLSGDHAAIAAWRHDQAVRRTAERRPDLLHPSVVLDGVEVRA
AEPADAGELYVLQLACWVQELHANPGVRIPALHESLEDLAAWLDRDTVLVARAGGRLVGAVRASLDEQTWEVGRLMVAPD
LAGRGLGRTLLTRIEALAPPEATTYALFTGAGSARNQRMYKKAGYRLRGEVEPGVVRMTKRISRR

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=229, Percent_Identity=46.2882096069869, Blast_Score=187, Evalue=1e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 42030; Mature: 42030

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIDIVSIFPEYLAPLELSLAGKAREKGLIDVRVHDLRAWTHDRHRTVDDTPYGGGAGMV
CEEEEEEHHHHHHCCEEEEECCCHHHCCEEEEEEEHHHHHHCCCCCCCCCCCCCCCCCEE
MKPEPWGEALDALGVLDGDGATVVVTTPSGQPFTQEVARELATRERLVFACGRYEGIDQR
ECCCCHHHHHHHHEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHEEECCCCCCHHHH
VLEEVAERAELREISLGDYVLNGGEVAALAITEAVVRLVPGFMGNAESLLEESHEGGLLE
HHHHHHHHHHHHCCCCCCEEECCCCEEHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEE
YPVYTKPGSWRGRAVPPVLLSGDHAAIAAWRHDQAVRRTAERRPDLLHPSVVLDGVEVRA
CCEEECCCCCCCCCCCCEEECCCCEEEEEHHHHHHHHHHHHHCCCCCCCHHEECCCEEEE
AEPADAGELYVLQLACWVQELHANPGVRIPALHESLEDLAAWLDRDTVLVARAGGRLVGA
CCCCCCCCEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCEEHH
VRASLDEQTWEVGRLMVAPDLAGRGLGRTLLTRIEALAPPEATTYALFTGAGSARNQRMY
HHHHHHHHHHHHCEEEECCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHH
KKAGYRLRGEVEPGVVRMTKRISRR
HHCCCEEECCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MRIDIVSIFPEYLAPLELSLAGKAREKGLIDVRVHDLRAWTHDRHRTVDDTPYGGGAGMV
CEEEEEEHHHHHHCCEEEEECCCHHHCCEEEEEEEHHHHHHCCCCCCCCCCCCCCCCCEE
MKPEPWGEALDALGVLDGDGATVVVTTPSGQPFTQEVARELATRERLVFACGRYEGIDQR
ECCCCHHHHHHHHEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHEEECCCCCCHHHH
VLEEVAERAELREISLGDYVLNGGEVAALAITEAVVRLVPGFMGNAESLLEESHEGGLLE
HHHHHHHHHHHHCCCCCCEEECCCCEEHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEE
YPVYTKPGSWRGRAVPPVLLSGDHAAIAAWRHDQAVRRTAERRPDLLHPSVVLDGVEVRA
CCEEECCCCCCCCCCCCEEECCCCEEEEEHHHHHHHHHHHHHCCCCCCCHHEECCCEEEE
AEPADAGELYVLQLACWVQELHANPGVRIPALHESLEDLAAWLDRDTVLVARAGGRLVGA
CCCCCCCCEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCEEHH
VRASLDEQTWEVGRLMVAPDLAGRGLGRTLLTRIEALAPPEATTYALFTGAGSARNQRMY
HHHHHHHHHHHHCEEEECCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHH
KKAGYRLRGEVEPGVVRMTKRISRR
HHCCCEEECCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA