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The map label for this gene is polA [H]

Identifier: 119717066

GI number: 119717066

Start: 3011763

End: 3012632

Strand: Reverse

Name: polA [H]

Synonym: Noca_2842

Alternate gene names: 119717066

Gene position: 3012632-3011763 (Counterclockwise)

Preceding gene: 119717067

Following gene: 119717065

Centisome position: 60.42

GC content: 73.79

Gene sequence:

>870_bases
ATGCTGCGCCAGATCCTCGAGGCGATCGACCGGTTCGCCCCGGACGCGGTGCTGTTCGGGCTCGACGACCGGCGCACGTC
GGTGCGCGAGCAGGCCTACCCGGCGTACAAGGCCGGTCGCGCCGAGAAGGACCCGGCCCTCGTCGACCAGCTGGAGCGCG
CGGCCGGGCTCCTGGACGCCCTCGGGATGCTGACCGTGACCCCGGACGGCCTCGAGGCCGACGACGTGAACGCCTCGGCG
GCGACCTGGGCCGAGCAGCACGGCTGGAGCTGCGTGCTGATCACCTCCGACCGCGACGCGTTCGCGCACATCAGCGAGCA
CACCCAGGTGCTGCGGCTGATCAGCGGCGGGATCAACGGCTCGCCGCTGCTGAACCCGGCCCGGCTGCGGACCATGTACG
GGGTCGCGGCCGAGCACTACCTGGAGTACGCCGCGCTCCGCGGCGACCCGAGCGACAACCTGCCGGGCGTGCCCGGCATC
GGCGAGAAGATCGCGCCGTTCCTGCTCTCCGAGATGGGCTCGATGCGGGCCGTGTGGGCCGACATCGACCACTGCGGCGG
CGCGACGCTGGTGGCGACCCTCGACTCCTACTGCGAGGAGGTCGGCCGGCCCCGGATCGGGGCCGCGGTGCTGAAGAAGC
TGGCGACACCGGCCGCTCGGGAGCGCTTCGAGTTCAACCTCGCGATCATGTCCGGGCGCACCGACCTCGACCTCGGCCTG
ACCCCGGACCTGCCCGGCAGCCCCGGCCTGCTGCCGCTCCACCCGGACCGGGTCTCGCGGGTCGTCGGCCACCTCGGCGT
CGCCGCGACCACCCAGCTGGCGCTCCGGGTGCTCACCGAGCCACCGGCGAGCGCCGGCGCGATGGGCTGA

Upstream 100 bases:

>100_bases
GACGCCCCGTCGCTGCTGCACCGCAAACCGCAACCACCACGCGCGGGTCCAGTCGGGGCTGCTGGACCACGGCGGCCGGC
CGGCCTGGGCGCTGCACGGC

Downstream 100 bases:

>100_bases
GCCCACCGGGGGAGGTCGGTGGGCGGGCGTCCGGTCCGTGGTGCCCGGGGACTGTTGGGGCAATGCCTGAACTTCTCGGC
CGAAACTTCGGCCCAACAGT

Product: 5'-3' exonuclease

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MLRQILEAIDRFAPDAVLFGLDDRRTSVREQAYPAYKAGRAEKDPALVDQLERAAGLLDALGMLTVTPDGLEADDVNASA
ATWAEQHGWSCVLITSDRDAFAHISEHTQVLRLISGGINGSPLLNPARLRTMYGVAAEHYLEYAALRGDPSDNLPGVPGI
GEKIAPFLLSEMGSMRAVWADIDHCGGATLVATLDSYCEEVGRPRIGAAVLKKLATPAARERFEFNLAIMSGRTDLDLGL
TPDLPGSPGLLPLHPDRVSRVVGHLGVAATTQLALRVLTEPPASAGAMG

Sequences:

>Translated_289_residues
MLRQILEAIDRFAPDAVLFGLDDRRTSVREQAYPAYKAGRAEKDPALVDQLERAAGLLDALGMLTVTPDGLEADDVNASA
ATWAEQHGWSCVLITSDRDAFAHISEHTQVLRLISGGINGSPLLNPARLRTMYGVAAEHYLEYAALRGDPSDNLPGVPGI
GEKIAPFLLSEMGSMRAVWADIDHCGGATLVATLDSYCEEVGRPRIGAAVLKKLATPAARERFEFNLAIMSGRTDLDLGL
TPDLPGSPGLLPLHPDRVSRVVGHLGVAATTQLALRVLTEPPASAGAMG
>Mature_289_residues
MLRQILEAIDRFAPDAVLFGLDDRRTSVREQAYPAYKAGRAEKDPALVDQLERAAGLLDALGMLTVTPDGLEADDVNASA
ATWAEQHGWSCVLITSDRDAFAHISEHTQVLRLISGGINGSPLLNPARLRTMYGVAAEHYLEYAALRGDPSDNLPGVPGI
GEKIAPFLLSEMGSMRAVWADIDHCGGATLVATLDSYCEEVGRPRIGAAVLKKLATPAARERFEFNLAIMSGRTDLDLGL
TPDLPGSPGLLPLHPDRVSRVVGHLGVAATTQLALRVLTEPPASAGAMG

Specific function: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity [H]

COG id: COG0258

COG function: function code L; 5'-3' exonuclease (including N-terminal domain of PolI)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1790294, Length=190, Percent_Identity=29.4736842105263, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI226510970, Length=166, Percent_Identity=26.5060240963855, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR018320
- InterPro:   IPR002298
- InterPro:   IPR008918
- InterPro:   IPR003583
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 30750; Mature: 30750

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRQILEAIDRFAPDAVLFGLDDRRTSVREQAYPAYKAGRAEKDPALVDQLERAAGLLDA
CHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHH
LGMLTVTPDGLEADDVNASAATWAEQHGWSCVLITSDRDAFAHISEHTQVLRLISGGING
HHHEEECCCCCCCCCCCCHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCC
SPLLNPARLRTMYGVAAEHYLEYAALRGDPSDNLPGVPGIGEKIAPFLLSEMGSMRAVWA
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHH
DIDHCGGATLVATLDSYCEEVGRPRIGAAVLKKLATPAARERFEFNLAIMSGRTDLDLGL
HHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHEEEEEEEEECCCCCCCCC
TPDLPGSPGLLPLHPDRVSRVVGHLGVAATTQLALRVLTEPPASAGAMG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLRQILEAIDRFAPDAVLFGLDDRRTSVREQAYPAYKAGRAEKDPALVDQLERAAGLLDA
CHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHH
LGMLTVTPDGLEADDVNASAATWAEQHGWSCVLITSDRDAFAHISEHTQVLRLISGGING
HHHEEECCCCCCCCCCCCHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCC
SPLLNPARLRTMYGVAAEHYLEYAALRGDPSDNLPGVPGIGEKIAPFLLSEMGSMRAVWA
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHH
DIDHCGGATLVATLDSYCEEVGRPRIGAAVLKKLATPAARERFEFNLAIMSGRTDLDLGL
HHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHEEEEEEEEECCCCCCCCC
TPDLPGSPGLLPLHPDRVSRVVGHLGVAATTQLALRVLTEPPASAGAMG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]