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The map label for this gene is xylF [H]

Identifier: 119716603

GI number: 119716603

Start: 2532004

End: 2533134

Strand: Direct

Name: xylF [H]

Synonym: Noca_2376

Alternate gene names: 119716603

Gene position: 2532004-2533134 (Clockwise)

Preceding gene: 119716601

Following gene: 119716604

Centisome position: 50.78

GC content: 67.9

Gene sequence:

>1131_bases
ATGGTCAGGATCCAGGGGGCGCGGCTCGCGTCCCTCGTTGCAGTAGGAGCGCTCGCCTTCGGGTCGCTCGCCGCGTGCGG
CAGCGACTCCGACGGCGGCGACACCGGCAGTGAGAGCACCAACGGCACCAGCGAGTCGTCCGCACCGGAGGTCACCGGGA
AGGTCGGCGTGATCCTTCCGGACTCGAAGACCTCGGTCCGCTGGGAGACGCAGGACCGGCCGAACCTCGAGGCCGCCTTC
AAGGCAGCGGGCCTGGAGTCGATCATCCAGAACGCCGAGGGCTCGCCGGACACGTTCGGTCAGCTCTGTGACCAGATGAT
CACCGAGGGCGTCCAGGTCCTCATCGAGACCGACCTCGACCCGGACTCGGGCAAGGCCTGCATCGACAAGGCCAAGAACG
CGGGCATCGCCGTCATCGACTACGACCGTCTCACCCTGGGCGGCGGCGCGGACTACTACGTGTCGTTCGACAACGTGAAG
GTCGGCGCGCTGCAGGGTCAGGGCCTCATCGACGCCCTCCAGGCCAAGGGCGAGAAGAGCGGCAACATCGCCTTCATCAA
CGGCGCCGCGACCGACAACAACGCCACGCTGTTCAAGCAGGGCTACGAGCAGGTCATCAAGGACGACGGCAGCTACACGG
TCGTCGGCGACCAGACCGGTGAATGGGACCCGGACACCGCCGGCAAGGTCTTCGACCAGTTCTACGCCGCGCACCCCGAC
CTGGTCGGGCTCGTCACCGCCAACGACGGGATGGCCGGTGGCGTCATCGCCCGTGAGAAGGCCGCGGGCGTCGAGGGCAA
GATCCTGGTGACCGGCCAGGACGCGACCGCCGAGGGTCTCGCCAACGTGCTGCTGGGCTACCAGGCCGGCACCGTCTTCA
AGGACACCTCGATCGAGGCCAAGGCCGCTGCCGACCTGGCGATCGCGATCATCAAGGGCGAGGACCCGTCGACGATCGCG
ACCGGCTCCGTCAACGACTCGGTGCTCGGTGTCGACGTGCCGTCGGTGCTCGCCACCCCGGTCTGGATCACCGCGGACAC
GGTCAAGGACGTGGTCGCCGCCGGCCAGGCGAACGCCGCCGACATCTGCAAGGGTATCGAGGACCTCTGCAAGAAGTACG
GAGTCGCGTGA

Upstream 100 bases:

>100_bases
GGTCCCCTTGCCGGGGGTGGTGGTGCTCAGTAAGTTGTCCGCCACAACATATCCGGTGATGGTCGTCACATCATCGGGTC
CAAGCCATGGAGGTCGAGGA

Downstream 100 bases:

>100_bases
CCGCCGTCGTGGTCGTCTGACAGCTTGAGACACGGGGGCCCGCCCGGCTGGGCGGGCTCCCGTTCCACGATCAACCCCGG
AGGGGACGCAAATGTCGAGT

Product: periplasmic binding protein/LacI transcriptional regulator

Products: ADP; phosphate; xylose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MVRIQGARLASLVAVGALAFGSLAACGSDSDGGDTGSESTNGTSESSAPEVTGKVGVILPDSKTSVRWETQDRPNLEAAF
KAAGLESIIQNAEGSPDTFGQLCDQMITEGVQVLIETDLDPDSGKACIDKAKNAGIAVIDYDRLTLGGGADYYVSFDNVK
VGALQGQGLIDALQAKGEKSGNIAFINGAATDNNATLFKQGYEQVIKDDGSYTVVGDQTGEWDPDTAGKVFDQFYAAHPD
LVGLVTANDGMAGGVIAREKAAGVEGKILVTGQDATAEGLANVLLGYQAGTVFKDTSIEAKAAADLAIAIIKGEDPSTIA
TGSVNDSVLGVDVPSVLATPVWITADTVKDVVAAGQANAADICKGIEDLCKKYGVA

Sequences:

>Translated_376_residues
MVRIQGARLASLVAVGALAFGSLAACGSDSDGGDTGSESTNGTSESSAPEVTGKVGVILPDSKTSVRWETQDRPNLEAAF
KAAGLESIIQNAEGSPDTFGQLCDQMITEGVQVLIETDLDPDSGKACIDKAKNAGIAVIDYDRLTLGGGADYYVSFDNVK
VGALQGQGLIDALQAKGEKSGNIAFINGAATDNNATLFKQGYEQVIKDDGSYTVVGDQTGEWDPDTAGKVFDQFYAAHPD
LVGLVTANDGMAGGVIAREKAAGVEGKILVTGQDATAEGLANVLLGYQAGTVFKDTSIEAKAAADLAIAIIKGEDPSTIA
TGSVNDSVLGVDVPSVLATPVWITADTVKDVVAAGQANAADICKGIEDLCKKYGVA
>Mature_376_residues
MVRIQGARLASLVAVGALAFGSLAACGSDSDGGDTGSESTNGTSESSAPEVTGKVGVILPDSKTSVRWETQDRPNLEAAF
KAAGLESIIQNAEGSPDTFGQLCDQMITEGVQVLIETDLDPDSGKACIDKAKNAGIAVIDYDRLTLGGGADYYVSFDNVK
VGALQGQGLIDALQAKGEKSGNIAFINGAATDNNATLFKQGYEQVIKDDGSYTVVGDQTGEWDPDTAGKVFDQFYAAHPD
LVGLVTANDGMAGGVIAREKAAGVEGKILVTGQDATAEGLANVLLGYQAGTVFKDTSIEAKAAADLAIAIIKGEDPSTIA
TGSVNDSVLGVDVPSVLATPVWITADTVKDVVAAGQANAADICKGIEDLCKKYGVA

Specific function: Involved in the high-affinity D-xylose membrane transport system. Binds with high affinity to xylose [H]

COG id: COG4213

COG function: function code G; ABC-type xylose transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1789990, Length=312, Percent_Identity=33.6538461538462, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI1790526, Length=155, Percent_Identity=33.5483870967742, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761
- InterPro:   IPR013456 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 38418; Mature: 38418

Theoretical pI: Translated: 4.03; Mature: 4.03

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00211 ABC_TRANSPORTER_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRIQGARLASLVAVGALAFGSLAACGSDSDGGDTGSESTNGTSESSAPEVTGKVGVILP
CEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEC
DSKTSVRWETQDRPNLEAAFKAAGLESIIQNAEGSPDTFGQLCDQMITEGVQVLIETDLD
CCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEEEECCCC
PDSGKACIDKAKNAGIAVIDYDRLTLGGGADYYVSFDNVKVGALQGQGLIDALQAKGEKS
CCCCHHHHHHHCCCCEEEEEECEEEECCCCEEEEEECCEEEEEECCCCHHHHHHHCCCCC
GNIAFINGAATDNNATLFKQGYEQVIKDDGSYTVVGDQTGEWDPDTAGKVFDQFYAAHPD
CCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHCCCC
LVGLVTANDGMAGGVIAREKAAGVEGKILVTGQDATAEGLANVLLGYQAGTVFKDTSIEA
EEEEEEECCCCCCCEEEHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCCEEECCCCCH
KAAADLAIAIIKGEDPSTIATGSVNDSVLGVDVPSVLATPVWITADTVKDVVAAGQANAA
HHCCEEEEEEEECCCCCEEEECCCCCCEEECCCHHHHHCCEEEEHHHHHHHHHCCCCCHH
DICKGIEDLCKKYGVA
HHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVRIQGARLASLVAVGALAFGSLAACGSDSDGGDTGSESTNGTSESSAPEVTGKVGVILP
CEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEC
DSKTSVRWETQDRPNLEAAFKAAGLESIIQNAEGSPDTFGQLCDQMITEGVQVLIETDLD
CCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEEEECCCC
PDSGKACIDKAKNAGIAVIDYDRLTLGGGADYYVSFDNVKVGALQGQGLIDALQAKGEKS
CCCCHHHHHHHCCCCEEEEEECEEEECCCCEEEEEECCEEEEEECCCCHHHHHHHCCCCC
GNIAFINGAATDNNATLFKQGYEQVIKDDGSYTVVGDQTGEWDPDTAGKVFDQFYAAHPD
CCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHCCCC
LVGLVTANDGMAGGVIAREKAAGVEGKILVTGQDATAEGLANVLLGYQAGTVFKDTSIEA
EEEEEEECCCCCCCEEEHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCCEEECCCCCH
KAAADLAIAIIKGEDPSTIATGSVNDSVLGVDVPSVLATPVWITADTVKDVVAAGQANAA
HHCCEEEEEEEECCCCCEEEECCCCCCEEECCCHHHHHCCEEEEHHHHHHHHHCCCCCHH
DICKGIEDLCKKYGVA
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; xylose [Periplasm]; H2O [C]

Specific reaction: ATP + xylose [Periplasm] + H2O = ADP + phosphate + xylose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 8581399; 9298646 [H]