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The map label for this gene is emrB [C]

Identifier: 119716399

GI number: 119716399

Start: 2320036

End: 2321700

Strand: Reverse

Name: emrB [C]

Synonym: Noca_2169

Alternate gene names: 119716399

Gene position: 2321700-2320036 (Counterclockwise)

Preceding gene: 119716400

Following gene: 119716394

Centisome position: 46.57

GC content: 65.59

Gene sequence:

>1665_bases
ATGAAGCAGGCACGCCCCCCGAGGTCGCAGTCGCAGAACGGTCGAAGCGAAACTCCTTCCGCGTCTCCTGGGGTAATCGG
AACCCAACCCGAGATGCCGCACCTGACCCACCGGCAGATTGTCACGGTGCTCGTCGGATTGATGCTGGGAATGCTCGTCG
CCGCGTTGTCGCAGACGATCGTGGCGACAGCTCTACCGACGATCGTCGGTGAGCTGGGCGGGCAGGACCAACTGGCCTGG
GTGGTCTCGGCGACACTGCTGACCGCGACCGCGTCCACACCCATCTGGGGAAAGCTCTCTGACCTCTACGGCCGCAAGAT
CATGTTCCAAGCGGCCATCGCGATCTTCCTCGTCTCCTCCCTCGCAGCAGGTTTCTCGCAAAACATGGCCCAGCTCGTTT
CGTTCCGAGCGGTGATGGGTATCGGTGTCGGCGGCTTGATGGCCTTGTCCCAGGCCATCATCGGTGACGTCGTCAGTCCG
CGGGAGCGCGGGCGGTACCAGGGCTACATCGGTTCGGTGTTCGGGCTCGCCACGGTGGCGGGGCCGCTGTTGGGTGGCTT
CCTGGTCGAGCACCTGTCGTGGCGTTGGTGCTTCTGGGTCGGGATCCCGATCGGGATCGTCGCGCTCGTGGTGACCGAAC
GGGTTCTCCAGCTCCCGTTCCCGCGCCGCCGCCATGCCATCGACTGGCTCGGCGCTTTCTTGATCGTGGCCGGGGTATCC
GCCCTGCTGCTCGTCCTGTCGCTTGGCGGCAAGGAGTTCGCCTGGAACTCCGCATGGACCTATGGCTTGACCGCCGCAGC
GATCGTCCTCCTCGCCCTTGCTGTGGTCCAGGAACGCCGAGCTGCCGAGCCGATCATGCCGCCACGCCTGTTCGCCAACC
ACACCTTCGTGATCACGAGCCTGGCCGGCTTCGTCATCGGGGTGGCCATGTTCGGCGCCATCATCTTCCTGCCGCAGTAC
CTCCAGATCGTCAAGGGCGAATCGCCGACCGCCTCCGGTCTGCAGACACTCCCCCTCATGGTGGGACTGCTGCTCACCTC
GATCGGATCCGGTCGGATCATCACCCGCACCGGTCGCTACAAGATTTTCCCCGTCGCCGGGATGTTCATCGCCGCCATCG
GGCTGGCATTGATGAGCACGCTGGACGTGGACACGTCGCTGTGGGTCGCCGGGATCTACATGTTCGTGACCGGCTTCGGG
ATCGGCATGACGATGCAGGTGCTGGTACTCGCCACCCAGAACGCCGTACCCCACGACGACCTGGGAGTGGCGACCTCAGG
CGCGACATTCTTCCGTTCCCTGGGCGGCGCGATGGGTGTCGCCATGTTCGGCGCCCTGCTTACGCACCGCCTCCGCGACA
CCATCCCGGCCCACCTCGCCTCAGCCGGCATCACACCCGACCAGATGCCGCAGGGATCACCCACCCAGGCCACCCCCCAA
CAGATCGATCAACTACCTGACGCCATCCACGCCGCGGTCACCGGTGGTTTCGCGGAAGCGCTCCAGACGACCTTCCTCGC
CGCCGTGCCCTTCGCCCTCGCCGGATTCGTGATCCTCCTCTTTTTGCACGAGACACCACTGCGACACAGCCGCGGGACCG
CCCGCGAGGACGACAGCCGCGGAGCCGGGAGTCTCGTGGCCGATACCTCTACCCACGGCGAGTGA

Upstream 100 bases:

>100_bases
GGCTCCCCACTGACCGCCGCCGGACCGCTTGGACGGGTGACGTGGGCCGCCTCTCCCAACCCTCTACGACGATGTGAACA
GCCAAGACAGGAAGTGGTCG

Downstream 100 bases:

>100_bases
TCCCGCTACCCACAGCGCAGCCCGGTCAGGTCGGTCACCCTCGAAGCGCCTCTGAGGCGCCCCGGAGGCGTTCGGTCAAC
GTGAACTCGAGCTCAGGGAG

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MKQARPPRSQSQNGRSETPSASPGVIGTQPEMPHLTHRQIVTVLVGLMLGMLVAALSQTIVATALPTIVGELGGQDQLAW
VVSATLLTATASTPIWGKLSDLYGRKIMFQAAIAIFLVSSLAAGFSQNMAQLVSFRAVMGIGVGGLMALSQAIIGDVVSP
RERGRYQGYIGSVFGLATVAGPLLGGFLVEHLSWRWCFWVGIPIGIVALVVTERVLQLPFPRRRHAIDWLGAFLIVAGVS
ALLLVLSLGGKEFAWNSAWTYGLTAAAIVLLALAVVQERRAAEPIMPPRLFANHTFVITSLAGFVIGVAMFGAIIFLPQY
LQIVKGESPTASGLQTLPLMVGLLLTSIGSGRIITRTGRYKIFPVAGMFIAAIGLALMSTLDVDTSLWVAGIYMFVTGFG
IGMTMQVLVLATQNAVPHDDLGVATSGATFFRSLGGAMGVAMFGALLTHRLRDTIPAHLASAGITPDQMPQGSPTQATPQ
QIDQLPDAIHAAVTGGFAEALQTTFLAAVPFALAGFVILLFLHETPLRHSRGTAREDDSRGAGSLVADTSTHGE

Sequences:

>Translated_554_residues
MKQARPPRSQSQNGRSETPSASPGVIGTQPEMPHLTHRQIVTVLVGLMLGMLVAALSQTIVATALPTIVGELGGQDQLAW
VVSATLLTATASTPIWGKLSDLYGRKIMFQAAIAIFLVSSLAAGFSQNMAQLVSFRAVMGIGVGGLMALSQAIIGDVVSP
RERGRYQGYIGSVFGLATVAGPLLGGFLVEHLSWRWCFWVGIPIGIVALVVTERVLQLPFPRRRHAIDWLGAFLIVAGVS
ALLLVLSLGGKEFAWNSAWTYGLTAAAIVLLALAVVQERRAAEPIMPPRLFANHTFVITSLAGFVIGVAMFGAIIFLPQY
LQIVKGESPTASGLQTLPLMVGLLLTSIGSGRIITRTGRYKIFPVAGMFIAAIGLALMSTLDVDTSLWVAGIYMFVTGFG
IGMTMQVLVLATQNAVPHDDLGVATSGATFFRSLGGAMGVAMFGALLTHRLRDTIPAHLASAGITPDQMPQGSPTQATPQ
QIDQLPDAIHAAVTGGFAEALQTTFLAAVPFALAGFVILLFLHETPLRHSRGTAREDDSRGAGSLVADTSTHGE
>Mature_554_residues
MKQARPPRSQSQNGRSETPSASPGVIGTQPEMPHLTHRQIVTVLVGLMLGMLVAALSQTIVATALPTIVGELGGQDQLAW
VVSATLLTATASTPIWGKLSDLYGRKIMFQAAIAIFLVSSLAAGFSQNMAQLVSFRAVMGIGVGGLMALSQAIIGDVVSP
RERGRYQGYIGSVFGLATVAGPLLGGFLVEHLSWRWCFWVGIPIGIVALVVTERVLQLPFPRRRHAIDWLGAFLIVAGVS
ALLLVLSLGGKEFAWNSAWTYGLTAAAIVLLALAVVQERRAAEPIMPPRLFANHTFVITSLAGFVIGVAMFGAIIFLPQY
LQIVKGESPTASGLQTLPLMVGLLLTSIGSGRIITRTGRYKIFPVAGMFIAAIGLALMSTLDVDTSLWVAGIYMFVTGFG
IGMTMQVLVLATQNAVPHDDLGVATSGATFFRSLGGAMGVAMFGALLTHRLRDTIPAHLASAGITPDQMPQGSPTQATPQ
QIDQLPDAIHAAVTGGFAEALQTTFLAAVPFALAGFVILLFLHETPLRHSRGTAREDDSRGAGSLVADTSTHGE

Specific function: Translocase That Confers Resistance To Substances Of High Hydrophobicity. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=460, Percent_Identity=27.6086956521739, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1788710, Length=477, Percent_Identity=26.4150943396226, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI87081983, Length=336, Percent_Identity=27.3809523809524, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI1790195, Length=407, Percent_Identity=26.5356265356265, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1788392, Length=416, Percent_Identity=27.1634615384615, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI48994889, Length=105, Percent_Identity=34.2857142857143, Blast_Score=64, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322958, Length=548, Percent_Identity=24.0875912408759, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6323735, Length=536, Percent_Identity=25.1865671641791, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6319770, Length=461, Percent_Identity=26.0303687635575, Blast_Score=136, Evalue=8e-33,
Organism=Saccharomyces cerevisiae, GI6325455, Length=449, Percent_Identity=23.8307349665924, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6319783, Length=462, Percent_Identity=23.3766233766234, Blast_Score=123, Evalue=9e-29,
Organism=Saccharomyces cerevisiae, GI6321663, Length=541, Percent_Identity=22.181146025878, Blast_Score=111, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6324954, Length=427, Percent_Identity=27.6346604215457, Blast_Score=101, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829
- InterPro:   IPR001958
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 58409; Mature: 58409

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCEEECCCCCCC
>Mature Secondary Structure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CCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]