Click here to switch to the map view.

The map label for this gene is mmsB [H]

Identifier: 119716390

GI number: 119716390

Start: 2308711

End: 2309637

Strand: Direct

Name: mmsB [H]

Synonym: Noca_2159

Alternate gene names: 119716390

Gene position: 2308711-2309637 (Clockwise)

Preceding gene: 119716385

Following gene: 119716391

Centisome position: 46.31

GC content: 62.89

Gene sequence:

>927_bases
ATGGAGCGCATCGCATTCATTGGACTCGGCAACATGGGCAACTCGATGGCGAAGAACCTGATCGCCGCCGGGTTCGACGT
GGTTGGGTTCGACGCCGACTCTCGTACGAACCAGCGTTCGGCCGAGACAGGCATCCCAGTCGCCGCGGACGTTGTGGACG
CCGTCACCGGCGCAGACGTTGTGATCACGATGCTCCCCAATGGCGACATCCTTCGCCAGGTGCTGCTGGGCGCTGGCGCC
GAATCTGGCGTCTTGAACTACCTCGCACCGGGGGCGCTCGTCATCGACTCATCCTCGATCGACGTCGCCAACTGTTTGGC
GGTACACGAGAGCGCGGCCTCGGCGGGCATCGCCTTCCTCGACGCTCCGGTCTCCGGCGGGGTCGGAGGCGCTGCGGCAG
GGACCCTCACCTTTATGGTGGGTGGGGACGAAGCACACGTGAGGCGAGCCTGGCCGCTCTTCGAGGCCATGGGCAAGAAG
GTCGTTCGAGTCGGCGGACCGTCGACCGGACAAGCCGTCAAGATGTGCAATCAGATGATCTACGCCTCGTCGCTCGTCGC
GGTTGCAGAAGCCTTCTCTCTCGCCGACAAACTCGGTGTCGCACCGAAGATGCTGTTCGAGGTAGTCACGAGCTCCTCAG
GAGACTGTTGGGCCATCCGCAACTCCTGCCCCTGGCCCGATGTTGTCGAAGGAGGATCAGCAGCCGACGAAGGCTTCGCC
CCACGCTTCTCCTCGCGGCTAATGAGCAAGGATCTTCGACTGGCTACCTCCGCAGCCTCGAACGTTGGCCAGCCTCTGCC
TCTGACGGGCAAGACTGTGACGTTCTACGAGAAGGTCGCCGTACATGAGCCGGCACTTGATGTCACTGCCGTGATCCGCG
AGATCAATGAAGCCGGCGCGTCTACTGCGACTGTAGTCACCCGATGA

Upstream 100 bases:

>100_bases
AGGAGGTGCACGCAGTTGGCCCTTGTCCTTGGACCTTGCTACGCCGAGTGTTGCGTCCAAATTCCTTACTCCAACACTGA
CTACGGGAGATGGCCTCGAC

Downstream 100 bases:

>100_bases
GCCTCGGCATCACCGCGCCGACAGACGCGATCTCGATGGACATGCAGATGCTCATCGGAGGCAACTGGGTGGACGCGCTG
GGCGAGGAACGCATCCCCGT

Product: 3-hydroxyisobutyrate dehydrogenase

Products: NA

Alternate protein names: HIBADH [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MERIAFIGLGNMGNSMAKNLIAAGFDVVGFDADSRTNQRSAETGIPVAADVVDAVTGADVVITMLPNGDILRQVLLGAGA
ESGVLNYLAPGALVIDSSSIDVANCLAVHESAASAGIAFLDAPVSGGVGGAAAGTLTFMVGGDEAHVRRAWPLFEAMGKK
VVRVGGPSTGQAVKMCNQMIYASSLVAVAEAFSLADKLGVAPKMLFEVVTSSSGDCWAIRNSCPWPDVVEGGSAADEGFA
PRFSSRLMSKDLRLATSAASNVGQPLPLTGKTVTFYEKVAVHEPALDVTAVIREINEAGASTATVVTR

Sequences:

>Translated_308_residues
MERIAFIGLGNMGNSMAKNLIAAGFDVVGFDADSRTNQRSAETGIPVAADVVDAVTGADVVITMLPNGDILRQVLLGAGA
ESGVLNYLAPGALVIDSSSIDVANCLAVHESAASAGIAFLDAPVSGGVGGAAAGTLTFMVGGDEAHVRRAWPLFEAMGKK
VVRVGGPSTGQAVKMCNQMIYASSLVAVAEAFSLADKLGVAPKMLFEVVTSSSGDCWAIRNSCPWPDVVEGGSAADEGFA
PRFSSRLMSKDLRLATSAASNVGQPLPLTGKTVTFYEKVAVHEPALDVTAVIREINEAGASTATVVTR
>Mature_308_residues
MERIAFIGLGNMGNSMAKNLIAAGFDVVGFDADSRTNQRSAETGIPVAADVVDAVTGADVVITMLPNGDILRQVLLGAGA
ESGVLNYLAPGALVIDSSSIDVANCLAVHESAASAGIAFLDAPVSGGVGGAAAGTLTFMVGGDEAHVRRAWPLFEAMGKK
VVRVGGPSTGQAVKMCNQMIYASSLVAVAEAFSLADKLGVAPKMLFEVVTSSSGDCWAIRNSCPWPDVVEGGSAADEGFA
PRFSSRLMSKDLRLATSAASNVGQPLPLTGKTVTFYEKVAVHEPALDVTAVIREINEAGASTATVVTR

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=295, Percent_Identity=37.9661016949153, Blast_Score=181, Evalue=6e-46,
Organism=Homo sapiens, GI40556376, Length=295, Percent_Identity=25.7627118644068, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI145693186, Length=275, Percent_Identity=34.5454545454545, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1790315, Length=306, Percent_Identity=32.0261437908497, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1786719, Length=296, Percent_Identity=32.7702702702703, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1789092, Length=278, Percent_Identity=31.6546762589928, Blast_Score=107, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17557316, Length=281, Percent_Identity=38.4341637010676, Blast_Score=177, Evalue=9e-45,
Organism=Drosophila melanogaster, GI24655230, Length=278, Percent_Identity=37.7697841726619, Blast_Score=167, Evalue=8e-42,
Organism=Drosophila melanogaster, GI19922568, Length=278, Percent_Identity=37.7697841726619, Blast_Score=167, Evalue=8e-42,
Organism=Drosophila melanogaster, GI28574115, Length=277, Percent_Identity=25.9927797833935, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR011548
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: =1.1.1.31 [H]

Molecular weight: Translated: 31531; Mature: 31531

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERIAFIGLGNMGNSMAKNLIAAGFDVVGFDADSRTNQRSAETGIPVAADVVDAVTGADV
CCEEEEEEECCCCHHHHHHHHHCCCEEEEECCCCCCCCCCHHCCCCHHHHHHHHHCCCCE
VITMLPNGDILRQVLLGAGAESGVLNYLAPGALVIDSSSIDVANCLAVHESAASAGIAFL
EEEECCCCHHHHHHHHCCCCCCCCHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCEEEE
DAPVSGGVGGAAAGTLTFMVGGDEAHVRRAWPLFEAMGKKVVRVGGPSTGQAVKMCNQMI
ECCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHH
YASSLVAVAEAFSLADKLGVAPKMLFEVVTSSSGDCWAIRNSCPWPDVVEGGSAADEGFA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEECCCCCCCHHHCCCCCCCCCCC
PRFSSRLMSKDLRLATSAASNVGQPLPLTGKTVTFYEKVAVHEPALDVTAVIREINEAGA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHCCCCHHHHHHHHHHHHCCC
STATVVTR
CCEEEEEC
>Mature Secondary Structure
MERIAFIGLGNMGNSMAKNLIAAGFDVVGFDADSRTNQRSAETGIPVAADVVDAVTGADV
CCEEEEEEECCCCHHHHHHHHHCCCEEEEECCCCCCCCCCHHCCCCHHHHHHHHHCCCCE
VITMLPNGDILRQVLLGAGAESGVLNYLAPGALVIDSSSIDVANCLAVHESAASAGIAFL
EEEECCCCHHHHHHHHCCCCCCCCHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCEEEE
DAPVSGGVGGAAAGTLTFMVGGDEAHVRRAWPLFEAMGKKVVRVGGPSTGQAVKMCNQMI
ECCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHH
YASSLVAVAEAFSLADKLGVAPKMLFEVVTSSSGDCWAIRNSCPWPDVVEGGSAADEGFA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEECCCCCCCHHHCCCCCCCCCCC
PRFSSRLMSKDLRLATSAASNVGQPLPLTGKTVTFYEKVAVHEPALDVTAVIREINEAGA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHCCCCHHHHHHHHHHHHCCC
STATVVTR
CCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]