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The map label for this gene is wtpB [H]

Identifier: 119716301

GI number: 119716301

Start: 2215289

End: 2216104

Strand: Direct

Name: wtpB [H]

Synonym: Noca_2070

Alternate gene names: 119716301

Gene position: 2215289-2216104 (Clockwise)

Preceding gene: 119716300

Following gene: 119716302

Centisome position: 44.43

GC content: 72.3

Gene sequence:

>816_bases
GTGACCACCGCCTCGCCATGGCGGGGTCGCGTCTGGGTGCTGGGAGCGGTCGTGCTGCTCTGGGCGCCCTTCCTCCCGCT
GCTGCTCCGGGCCGTCGCCGCGAACTCGCCCTATCCCGAGGTCGTCCCGTCCACGTTCACCGGGCGCGGGCTGGTGCAGG
CCTTCGCCCCCTCCGGGCGGGTGCTCGAGGGGCTGGTGCTCTCCGTGCTGACGGCGAGCGTGGTGGCCGTCCTGGCGTGC
GCCGTCGCCTGGCCGGCCGGTCGCGCCCTGGGGCAGCACCGGTTCCGTGGCCGGGGCTTCATCAAACTGCTGCTGCTCGC
GCCGGTCGTCGTGCCACCGATGGCCGCGACCATGGGTCTGCAGGTCTTCTTCATCCGCCTCGGTCTCGCCCACACCCCGT
TCGGCGTGGCGCTGGTGCAGCTGGTCCCGGCGCTTCCCTACGCGATCTTCGTCCTCGAGGCGGGCTTCGCGAACTTCGAC
CGCTCCTACGAGCGGCAGGCCCGAGCGCTCGGCGCCCGACGGCTGCGCCGCTGGTGGTCACTGACGCTCCCGCTGATGCT
GCCCTGGCTGGTCGCTGCCGCCGTGCTCGCCTTCCTCGTCTCCTGGAGCGACTACACGCTGACCCTGCTGGTGGGTGGGG
GACAGGTGCAGACCCTGCCGATCCTGCTGTTCGCCGCTGTGGGTTCGTCCGACACGACCCTGGCTGCGTCGCTGGCGCTG
CTGGTGATGCTGCCGCCGATCGTGCTGACCGCTGCCGCGGCCAGGCAGCTGTCCGGGGGGGCTCCTGCCGAGATCGCGAT
CGGAGGTCTGGGATGA

Upstream 100 bases:

>100_bases
GTCCGCAAGCCATGGCGCTTGCCATGGTCGCGACGGTGATCAGCGTGGCGGGTGCGGCGGGGTACGTCTTGCTGTTCACC
CGCCTCGTGCGGAGACCACT

Downstream 100 bases:

>100_bases
CCGGCGCGGTGATCGGCGCCCGGGTGCGCCTGGCCGGCCTGTCGAAGACGTACGACGGCGTGTGCGCCGTCGAGCAGGCG
GACCTCGAGCTCGCACCGGG

Product: binding-protein-dependent transport systems inner membrane component

Products: Phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MTTASPWRGRVWVLGAVVLLWAPFLPLLLRAVAANSPYPEVVPSTFTGRGLVQAFAPSGRVLEGLVLSVLTASVVAVLAC
AVAWPAGRALGQHRFRGRGFIKLLLLAPVVVPPMAATMGLQVFFIRLGLAHTPFGVALVQLVPALPYAIFVLEAGFANFD
RSYERQARALGARRLRRWWSLTLPLMLPWLVAAAVLAFLVSWSDYTLTLLVGGGQVQTLPILLFAAVGSSDTTLAASLAL
LVMLPPIVLTAAAARQLSGGAPAEIAIGGLG

Sequences:

>Translated_271_residues
MTTASPWRGRVWVLGAVVLLWAPFLPLLLRAVAANSPYPEVVPSTFTGRGLVQAFAPSGRVLEGLVLSVLTASVVAVLAC
AVAWPAGRALGQHRFRGRGFIKLLLLAPVVVPPMAATMGLQVFFIRLGLAHTPFGVALVQLVPALPYAIFVLEAGFANFD
RSYERQARALGARRLRRWWSLTLPLMLPWLVAAAVLAFLVSWSDYTLTLLVGGGQVQTLPILLFAAVGSSDTTLAASLAL
LVMLPPIVLTAAAARQLSGGAPAEIAIGGLG
>Mature_270_residues
TTASPWRGRVWVLGAVVLLWAPFLPLLLRAVAANSPYPEVVPSTFTGRGLVQAFAPSGRVLEGLVLSVLTASVVAVLACA
VAWPAGRALGQHRFRGRGFIKLLLLAPVVVPPMAATMGLQVFFIRLGLAHTPFGVALVQLVPALPYAIFVLEAGFANFDR
SYERQARALGARRLRRWWSLTLPLMLPWLVAAAVLAFLVSWSDYTLTLLVGGGQVQTLPILLFAAVGSSDTTLAASLALL
VMLPPIVLTAAAARQLSGGAPAEIAIGGLG

Specific function: Part of the ABC transporter complex wtpABC involved in molybdate/tungstate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0395

COG function: function code G; ABC-type sugar transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787368, Length=180, Percent_Identity=24.4444444444444, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 28591; Mature: 28459

Theoretical pI: Translated: 11.97; Mature: 11.97

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTASPWRGRVWVLGAVVLLWAPFLPLLLRAVAANSPYPEVVPSTFTGRGLVQAFAPSGR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCH
VLEGLVLSVLTASVVAVLACAVAWPAGRALGQHRFRGRGFIKLLLLAPVVVPPMAATMGL
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHH
QVFFIRLGLAHTPFGVALVQLVPALPYAIFVLEAGFANFDRSYERQARALGARRLRRWWS
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHCCHHHHHHHHHHHHHHHHHHHH
LTLPLMLPWLVAAAVLAFLVSWSDYTLTLLVGGGQVQTLPILLFAAVGSSDTTLAASLAL
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHH
LVMLPPIVLTAAAARQLSGGAPAEIAIGGLG
HHHHHHHHHHHHHHHHHCCCCCCEEEECCCC
>Mature Secondary Structure 
TTASPWRGRVWVLGAVVLLWAPFLPLLLRAVAANSPYPEVVPSTFTGRGLVQAFAPSGR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCH
VLEGLVLSVLTASVVAVLACAVAWPAGRALGQHRFRGRGFIKLLLLAPVVVPPMAATMGL
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHH
QVFFIRLGLAHTPFGVALVQLVPALPYAIFVLEAGFANFDRSYERQARALGARRLRRWWS
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHCCHHHHHHHHHHHHHHHHHHHH
LTLPLMLPWLVAAAVLAFLVSWSDYTLTLLVGGGQVQTLPILLFAAVGSSDTTLAASLAL
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHH
LVMLPPIVLTAAAARQLSGGAPAEIAIGGLG
HHHHHHHHHHHHHHHHHCCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]