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The map label for this gene is nadE [H]

Identifier: 119716275

GI number: 119716275

Start: 2187588

End: 2189633

Strand: Direct

Name: nadE [H]

Synonym: Noca_2044

Alternate gene names: 119716275

Gene position: 2187588-2189633 (Clockwise)

Preceding gene: 119716274

Following gene: 119716276

Centisome position: 43.88

GC content: 71.31

Gene sequence:

>2046_bases
GTGGACTTCTACTCCGCCTACGCCCATGGCTTCGCCCGGGTCGCCGCCTGCACGGTGCCGATCCGGGTGGCCGACCCCGC
GACCAACGCCCGGGCCGTGCTGGAGCAGGCCCAGGCGTGCTCCGACGAGGGGGTCGCGGTCGCGATCTTCCCCGAGCTCT
GCCTGAGCGGGTACGCCCTCGACGACCTGTTCCTGCAGGACGTGCTGCTCGACGCGGTCGCCGACGAGATCGCCGGCCTG
GTCGCCGCGTCGGAGGGCCTGCTGCCGGTGCTCGTCGTGGGCGCACCCGTCGCGCACGGCAGCCGGGTGCTGAACGCCGC
GGTCGTCATCCACCGCGGCCGGATCCTCGGCGTTGCGCCGAAGTCCTACCTGCCGACGTACCGCGAGTTCTACGAGCGCC
GCTGGTTCGCGCCCGGCGACGACGTGCGCGGCTCGATGGTGCTGGCCGGCCAGGAGGTGCCGGTCGGGCCGGACCTGCTC
TTCGAGGCCGAGGACGTACGCGGGCTGGTGCTGCACGTCGAGGTCTGCGAGGACATGTGGGTGCCGGTGCCGCCGAGCGC
CGAGGCGGCGCTCGCCGGCGCCACCGTGCTCGCCAACATCTCCGGCAGCCCGATCACCGTCGGCCGGGCCAGCGACCGGC
ACCTGCTGGCGCGCAGCGCGTCCAGCCGCTGCCTCGCGGCGTACCTCTATGCCGCCGGCGGCCAGGGCGAGTCCACGACC
GACTTGTCGTGGGACGGGCAGACGATGGTCTACGAGCACGGCGAGCTGCTCGCCGAGACCGAGCGGTTCCCCGACGGACC
GCGCGCAGCGATCGTCGACCTCGACCTCGACCGCACCCGCCTCGAGCGGCTCCGGACCGGGACGTTCGACGACAACCGCC
GCACGATCGGACCGGAGTTCCGCACCGTCGACTTCGAGCTCGGCCCGCCGACCAAGGACATCGGGCTGCGCCGCAAGGTG
GACCGCTTCCCGTTCGTGCCCGACGACGCCGAGCGGCTGGCCCAGGACTGCTACGAGGCCTACAACATCCAGGTCTCGGG
CCTCGAGCAGCGGCTCTCGGCCATCGGGGAGGGGCACGTCGGCCAGCCGAAGGTCGTGATCGGCATCAGCGGCGGCCTGG
ACTCCACCCACGCGCTGATCGTCGCGGCGAAGGCGATGGACCGGCTCGGCCGCGACCGCAGCGAGATCCTCGGCTTCACG
CTGCCCGGATTCGCGACCGGCGAGGCCACCAAGGGCTATGCGTGGGCGCTCGCCGACTCGCTCGGCATCACCATGCAGGA
GATCGACATCACCGACGCCGCGCGGGCGATGCTCACCGACCTCGACCACCCCTACGCCAAGGGGGAGGAGGTCTACGACA
TCACCTTCGAGAACGTCCAGGCCGGGCTGCGCACCGACTACCTGTTCCGGCTGGCCAACCACCGCGGCGGCATCGTGCTC
GGCACCGGTGACCTCTCCGAGCTGGCGCTGGGCTGGTGCACGTACGGCGTGGGCGACCAGATGTCCCACTACAACGTCAA
CGCCGGCGTACCGAAGACGCTGATCCAGCACCTGATCCGTTGGGTGATCAGCACCGGCCAGTTCGACGAGAGCACCAACG
CGGTGCTCCTCGACGTGGTCGGCCAGGAGATCACTCCGGAGCTGATCCCCACCCGGGAGGACCACCTGCCGCAGTCCACC
GAGGAGTCGGTCGGTCCGTACTCCCTGCAGGACTTCACGCTGTTCCACGTGCTGCGCTACGGCTACGCACCCAGCAAGAT
CGCGTTCCTCGCCTGGCACGCCTGGCACGACGCCGACGCGGGGGAGTGGCCGCCGAACTACCCCGACGACCGGCGCATCG
AGTTCGACCTCGCCACCATCCGGCGTTGGCTCGAGGTGTTCGTGCGGCGGTTCTTCGCCAGCCAGTTCAAGCGTTCCGCG
CTGCCGAACGGGCCCAAGGTCAGCCACGGCGGCACGATGTCCCCGCGCGGTGACTGGCGGATGCCCTCGGACGCCTCGCC
GGCCGCGTGGCTGGCCGAGCTGGAGGCCAACGTCCCGACGAGCTGA

Upstream 100 bases:

>100_bases
GCTCCCCGACGGCGCCTTCACGGTCGACGCGACGGCTTGGACGGTCCTCGGGCGCGCCTGACCCACGAATGCGGGGGCGC
CGCGTGGGAAACTCCGGAGC

Downstream 100 bases:

>100_bases
GAAATGCTGCTCCGGCAGGCAACCGAACCGGCGGCGCAGGCGTCACCACCGTGACACGATCTAGATCCTGCCCCGAGGAT
CCAGCCAGAGGAGCAACGCC

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 681; Mature: 681

Protein sequence:

>681_residues
MDFYSAYAHGFARVAACTVPIRVADPATNARAVLEQAQACSDEGVAVAIFPELCLSGYALDDLFLQDVLLDAVADEIAGL
VAASEGLLPVLVVGAPVAHGSRVLNAAVVIHRGRILGVAPKSYLPTYREFYERRWFAPGDDVRGSMVLAGQEVPVGPDLL
FEAEDVRGLVLHVEVCEDMWVPVPPSAEAALAGATVLANISGSPITVGRASDRHLLARSASSRCLAAYLYAAGGQGESTT
DLSWDGQTMVYEHGELLAETERFPDGPRAAIVDLDLDRTRLERLRTGTFDDNRRTIGPEFRTVDFELGPPTKDIGLRRKV
DRFPFVPDDAERLAQDCYEAYNIQVSGLEQRLSAIGEGHVGQPKVVIGISGGLDSTHALIVAAKAMDRLGRDRSEILGFT
LPGFATGEATKGYAWALADSLGITMQEIDITDAARAMLTDLDHPYAKGEEVYDITFENVQAGLRTDYLFRLANHRGGIVL
GTGDLSELALGWCTYGVGDQMSHYNVNAGVPKTLIQHLIRWVISTGQFDESTNAVLLDVVGQEITPELIPTREDHLPQST
EESVGPYSLQDFTLFHVLRYGYAPSKIAFLAWHAWHDADAGEWPPNYPDDRRIEFDLATIRRWLEVFVRRFFASQFKRSA
LPNGPKVSHGGTMSPRGDWRMPSDASPAAWLAELEANVPTS

Sequences:

>Translated_681_residues
MDFYSAYAHGFARVAACTVPIRVADPATNARAVLEQAQACSDEGVAVAIFPELCLSGYALDDLFLQDVLLDAVADEIAGL
VAASEGLLPVLVVGAPVAHGSRVLNAAVVIHRGRILGVAPKSYLPTYREFYERRWFAPGDDVRGSMVLAGQEVPVGPDLL
FEAEDVRGLVLHVEVCEDMWVPVPPSAEAALAGATVLANISGSPITVGRASDRHLLARSASSRCLAAYLYAAGGQGESTT
DLSWDGQTMVYEHGELLAETERFPDGPRAAIVDLDLDRTRLERLRTGTFDDNRRTIGPEFRTVDFELGPPTKDIGLRRKV
DRFPFVPDDAERLAQDCYEAYNIQVSGLEQRLSAIGEGHVGQPKVVIGISGGLDSTHALIVAAKAMDRLGRDRSEILGFT
LPGFATGEATKGYAWALADSLGITMQEIDITDAARAMLTDLDHPYAKGEEVYDITFENVQAGLRTDYLFRLANHRGGIVL
GTGDLSELALGWCTYGVGDQMSHYNVNAGVPKTLIQHLIRWVISTGQFDESTNAVLLDVVGQEITPELIPTREDHLPQST
EESVGPYSLQDFTLFHVLRYGYAPSKIAFLAWHAWHDADAGEWPPNYPDDRRIEFDLATIRRWLEVFVRRFFASQFKRSA
LPNGPKVSHGGTMSPRGDWRMPSDASPAAWLAELEANVPTS
>Mature_681_residues
MDFYSAYAHGFARVAACTVPIRVADPATNARAVLEQAQACSDEGVAVAIFPELCLSGYALDDLFLQDVLLDAVADEIAGL
VAASEGLLPVLVVGAPVAHGSRVLNAAVVIHRGRILGVAPKSYLPTYREFYERRWFAPGDDVRGSMVLAGQEVPVGPDLL
FEAEDVRGLVLHVEVCEDMWVPVPPSAEAALAGATVLANISGSPITVGRASDRHLLARSASSRCLAAYLYAAGGQGESTT
DLSWDGQTMVYEHGELLAETERFPDGPRAAIVDLDLDRTRLERLRTGTFDDNRRTIGPEFRTVDFELGPPTKDIGLRRKV
DRFPFVPDDAERLAQDCYEAYNIQVSGLEQRLSAIGEGHVGQPKVVIGISGGLDSTHALIVAAKAMDRLGRDRSEILGFT
LPGFATGEATKGYAWALADSLGITMQEIDITDAARAMLTDLDHPYAKGEEVYDITFENVQAGLRTDYLFRLANHRGGIVL
GTGDLSELALGWCTYGVGDQMSHYNVNAGVPKTLIQHLIRWVISTGQFDESTNAVLLDVVGQEITPELIPTREDHLPQST
EESVGPYSLQDFTLFHVLRYGYAPSKIAFLAWHAWHDADAGEWPPNYPDDRRIEFDLATIRRWLEVFVRRFFASQFKRSA
LPNGPKVSHGGTMSPRGDWRMPSDASPAAWLAELEANVPTS

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI41393551, Length=613, Percent_Identity=23.3278955954323, Blast_Score=96, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17538756, Length=620, Percent_Identity=22.5806451612903, Blast_Score=89, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6321865, Length=687, Percent_Identity=22.1251819505095, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24641841, Length=626, Percent_Identity=23.961661341853, Blast_Score=81, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24641843, Length=626, Percent_Identity=23.961661341853, Blast_Score=81, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 74290; Mature: 74290

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFYSAYAHGFARVAACTVPIRVADPATNARAVLEQAQACSDEGVAVAIFPELCLSGYAL
CCHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEHHHHHCCCCH
DDLFLQDVLLDAVADEIAGLVAASEGLLPVLVVGAPVAHGSRVLNAAVVIHRGRILGVAP
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHEEEEEECCEEEEECC
KSYLPTYREFYERRWFAPGDDVRGSMVLAGQEVPVGPDLLFEAEDVRGLVLHVEVCEDMW
HHHCCHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCEEECCCCCEEEEEEEEHHCCC
VPVPPSAEAALAGATVLANISGSPITVGRASDRHLLARSASSRCLAAYLYAAGGQGESTT
CCCCCCCHHHHHCCEEEEECCCCCEEEECCCCCHHHHHCCCCHHHHHHHHHCCCCCCCCC
DLSWDGQTMVYEHGELLAETERFPDGPRAAIVDLDLDRTRLERLRTGTFDDNRRTIGPEF
CCCCCCCEEEEECCHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCE
RTVDFELGPPTKDIGLRRKVDRFPFVPDDAERLAQDCYEAYNIQVSGLEQRLSAIGEGHV
EEEEEECCCCCHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHCEEEECHHHHHHHHCCCCC
GQPKVVIGISGGLDSTHALIVAAKAMDRLGRDRSEILGFTLPGFATGEATKGYAWALADS
CCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHCEEECCCCCCCCCCCCEEEEEHHH
LGITMQEIDITDAARAMLTDLDHPYAKGEEVYDITFENVQAGLRTDYLFRLANHRGGIVL
HCCEEEEECHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHCCCHHHHHHHHHCCCCCEEE
GTGDLSELALGWCTYGVGDQMSHYNVNAGVPKTLIQHLIRWVISTGQFDESTNAVLLDVV
ECCCHHHHHHHHHHCCCCCCCCCEECCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEC
GQEITPELIPTREDHLPQSTEESVGPYSLQDFTLFHVLRYGYAPSKIAFLAWHAWHDADA
CCCCCCCCCCCCHHCCCCCCHHCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCC
GEWPPNYPDDRRIEFDLATIRRWLEVFVRRFFASQFKRSALPNGPKVSHGGTMSPRGDWR
CCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
MPSDASPAAWLAELEANVPTS
CCCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MDFYSAYAHGFARVAACTVPIRVADPATNARAVLEQAQACSDEGVAVAIFPELCLSGYAL
CCHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEHHHHHCCCCH
DDLFLQDVLLDAVADEIAGLVAASEGLLPVLVVGAPVAHGSRVLNAAVVIHRGRILGVAP
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHEEEEEECCEEEEECC
KSYLPTYREFYERRWFAPGDDVRGSMVLAGQEVPVGPDLLFEAEDVRGLVLHVEVCEDMW
HHHCCHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCEEECCCCCEEEEEEEEHHCCC
VPVPPSAEAALAGATVLANISGSPITVGRASDRHLLARSASSRCLAAYLYAAGGQGESTT
CCCCCCCHHHHHCCEEEEECCCCCEEEECCCCCHHHHHCCCCHHHHHHHHHCCCCCCCCC
DLSWDGQTMVYEHGELLAETERFPDGPRAAIVDLDLDRTRLERLRTGTFDDNRRTIGPEF
CCCCCCCEEEEECCHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCE
RTVDFELGPPTKDIGLRRKVDRFPFVPDDAERLAQDCYEAYNIQVSGLEQRLSAIGEGHV
EEEEEECCCCCHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHCEEEECHHHHHHHHCCCCC
GQPKVVIGISGGLDSTHALIVAAKAMDRLGRDRSEILGFTLPGFATGEATKGYAWALADS
CCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHCEEECCCCCCCCCCCCEEEEEHHH
LGITMQEIDITDAARAMLTDLDHPYAKGEEVYDITFENVQAGLRTDYLFRLANHRGGIVL
HCCEEEEECHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHCCCHHHHHHHHHCCCCCEEE
GTGDLSELALGWCTYGVGDQMSHYNVNAGVPKTLIQHLIRWVISTGQFDESTNAVLLDVV
ECCCHHHHHHHHHHCCCCCCCCCEECCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEC
GQEITPELIPTREDHLPQSTEESVGPYSLQDFTLFHVLRYGYAPSKIAFLAWHAWHDADA
CCCCCCCCCCCCHHCCCCCCHHCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCC
GEWPPNYPDDRRIEFDLATIRRWLEVFVRRFFASQFKRSALPNGPKVSHGGTMSPRGDWR
CCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
MPSDASPAAWLAELEANVPTS
CCCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]