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The map label for this gene is tyrS [H]

Identifier: 119716211

GI number: 119716211

Start: 2110631

End: 2111902

Strand: Direct

Name: tyrS [H]

Synonym: Noca_1979

Alternate gene names: 119716211

Gene position: 2110631-2111902 (Clockwise)

Preceding gene: 119716207

Following gene: 119716212

Centisome position: 42.33

GC content: 70.05

Gene sequence:

>1272_bases
GTGTCCATCGACCCGACTCTCCTCGACGACCTCGAGTGGCGCGGCCTGATCGCGCACTCGACGGACCTCGACGCCCTGCG
CGAGGCCCTGGCCGGGGGGAGTGTGCGGTTCTATGTCGGGTTCGACCCGACGGCGCCGAGCCTGCACCTGGGCAACCTGG
TGCAGATCCTCACCGCCAAGCGGCTGCAGGAGGCCGGGCACACGCCGTACGCCCTGGTCGGGGGAGCCACCGGCATGATC
GGCGATCCCAAGGAGTCCGGCGAGCGGACCCTCAACTCGCTCGACACGGTCAAGGAGTGGGTCGAGCGGGTGCGCCGCCA
GATCGAGCCGTTCCTGTCCTTCGAGGGCGCCAACGCCGCCACGATGGTGAACAACTACGACTGGACCGCCAGCCTGTCGA
CCATCGACTTCCTGCGTGACATCGGCAAGCACTTCCCGGTCAACCGGATGCTCTCGCGCGACGTCGTGCGCAGCCGCCTG
GACGCCGGCATCAGCTACACCGAGTTCAGCTACGTGCTGCTCCAGTCGATGGACTTCCTCAACCTCTACCGCGACCACGG
CGTGGTGCTGCAGTTCGGCGGCTCCGACCAATGGGGCAACCTCACCGGCGGCGTCGAGCTGATCCGTCGTGCCGCCGGTG
GCAAGGCGCACGCCTTCGCGACACCGCTGATCACCAAGGCCGACGGCACGAAGTACGGCAAGACCGAGGGCGGCGCGCTC
TGGCTGGACCCCGAGATGATGTCGCCGTACGCCTTCTACCAGTTCTGGCTCAACGTCGAGGACGAGAAGGTCGGCGAGCT
GCTGCGCGTCTTCACCTTCCTGCCGCGCGCCGAGATCGAGGCGCTCGAGGCCGAGCACGCGGAGAAGCCGTTCCTGCGCG
CCGGTCAGCGCGCGCTGGCCGAGCACGTCACCACCCTGGTCCACGGCGCCGAGGAGACCGAGCGGATCAGGGCGGCCTCC
GCCGCCCTGTTCGGCGGCGGCGACCTCACGACGCTCAGCGCCGCGACGTTGGGCGCCGCGCTGCGCGAGGCCGGCAGCGC
GTCCGTCGACGGGGACGAGGACCTCCCGACGGTGGTCGACCTGCTGGTCGCGACCGGGCTGGCGAAGAGCAAGGGCGACG
CGCGCCGGACCGTCACCGAGGGTGGTGCCTACCTCAACAATGCGCGGGTCGAGGATCCCGACCACGTCCCGGCCCCGGAG
GACCTGATCGACGGCACCTGGCTGGTGCTGCGGCGGGGCAAGAAGAACTTCGCCGGCGTCGAGGTGCGCTGA

Upstream 100 bases:

>100_bases
TGGGCGGCGGCGGCCGCCATGGGGCAGGCTTGACCCGCACGTACCCATCCGAGGATCCACCTCACCGTCCAGGTGACGAT
CCACCAGCGAAAGGTCCACC

Downstream 100 bases:

>100_bases
GCGTGGCGCCGGCGGCCGAGCGGGATCCGCGCTGGCTCGACACGCTGGCCGAGCGGGCCCGCGACCGGCTGCCGGCGTCC
GTCCTGGAGTACGTGCTGCA

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MSIDPTLLDDLEWRGLIAHSTDLDALREALAGGSVRFYVGFDPTAPSLHLGNLVQILTAKRLQEAGHTPYALVGGATGMI
GDPKESGERTLNSLDTVKEWVERVRRQIEPFLSFEGANAATMVNNYDWTASLSTIDFLRDIGKHFPVNRMLSRDVVRSRL
DAGISYTEFSYVLLQSMDFLNLYRDHGVVLQFGGSDQWGNLTGGVELIRRAAGGKAHAFATPLITKADGTKYGKTEGGAL
WLDPEMMSPYAFYQFWLNVEDEKVGELLRVFTFLPRAEIEALEAEHAEKPFLRAGQRALAEHVTTLVHGAEETERIRAAS
AALFGGGDLTTLSAATLGAALREAGSASVDGDEDLPTVVDLLVATGLAKSKGDARRTVTEGGAYLNNARVEDPDHVPAPE
DLIDGTWLVLRRGKKNFAGVEVR

Sequences:

>Translated_423_residues
MSIDPTLLDDLEWRGLIAHSTDLDALREALAGGSVRFYVGFDPTAPSLHLGNLVQILTAKRLQEAGHTPYALVGGATGMI
GDPKESGERTLNSLDTVKEWVERVRRQIEPFLSFEGANAATMVNNYDWTASLSTIDFLRDIGKHFPVNRMLSRDVVRSRL
DAGISYTEFSYVLLQSMDFLNLYRDHGVVLQFGGSDQWGNLTGGVELIRRAAGGKAHAFATPLITKADGTKYGKTEGGAL
WLDPEMMSPYAFYQFWLNVEDEKVGELLRVFTFLPRAEIEALEAEHAEKPFLRAGQRALAEHVTTLVHGAEETERIRAAS
AALFGGGDLTTLSAATLGAALREAGSASVDGDEDLPTVVDLLVATGLAKSKGDARRTVTEGGAYLNNARVEDPDHVPAPE
DLIDGTWLVLRRGKKNFAGVEVR
>Mature_422_residues
SIDPTLLDDLEWRGLIAHSTDLDALREALAGGSVRFYVGFDPTAPSLHLGNLVQILTAKRLQEAGHTPYALVGGATGMIG
DPKESGERTLNSLDTVKEWVERVRRQIEPFLSFEGANAATMVNNYDWTASLSTIDFLRDIGKHFPVNRMLSRDVVRSRLD
AGISYTEFSYVLLQSMDFLNLYRDHGVVLQFGGSDQWGNLTGGVELIRRAAGGKAHAFATPLITKADGTKYGKTEGGALW
LDPEMMSPYAFYQFWLNVEDEKVGELLRVFTFLPRAEIEALEAEHAEKPFLRAGQRALAEHVTTLVHGAEETERIRAASA
ALFGGGDLTTLSAATLGAALREAGSASVDGDEDLPTVVDLLVATGLAKSKGDARRTVTEGGAYLNNARVEDPDHVPAPED
LIDGTWLVLRRGKKNFAGVEVR

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=394, Percent_Identity=34.5177664974619, Blast_Score=206, Evalue=2e-53,
Organism=Escherichia coli, GI1787925, Length=418, Percent_Identity=48.5645933014354, Blast_Score=395, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI25145802, Length=402, Percent_Identity=35.0746268656716, Blast_Score=198, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI25145805, Length=297, Percent_Identity=34.006734006734, Blast_Score=149, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6325160, Length=444, Percent_Identity=35.3603603603604, Blast_Score=257, Evalue=2e-69,
Organism=Drosophila melanogaster, GI20130383, Length=412, Percent_Identity=37.621359223301, Blast_Score=258, Evalue=5e-69,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 46071; Mature: 45940

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIDPTLLDDLEWRGLIAHSTDLDALREALAGGSVRFYVGFDPTAPSLHLGNLVQILTAK
CCCCCCHHHCCCCCEEEECCCCHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHH
RLQEAGHTPYALVGGATGMIGDPKESGERTLNSLDTVKEWVERVRRQIEPFLSFEGANAA
HHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
TMVNNYDWTASLSTIDFLRDIGKHFPVNRMLSRDVVRSRLDAGISYTEFSYVLLQSMDFL
EEECCCCCEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
NLYRDHGVVLQFGGSDQWGNLTGGVELIRRAAGGKAHAFATPLITKADGTKYGKTEGGAL
HHHHCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCEEECCCEEECCCCCCCCCCCCCEE
WLDPEMMSPYAFYQFWLNVEDEKVGELLRVFTFLPRAEIEALEAEHAEKPFLRAGQRALA
EECHHHHCCCEEHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHH
EHVTTLVHGAEETERIRAASAALFGGGDLTTLSAATLGAALREAGSASVDGDEDLPTVVD
HHHHHHHHCCHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
LLVATGLAKSKGDARRTVTEGGAYLNNARVEDPDHVPAPEDLIDGTWLVLRRGKKNFAGV
HHHHHCCCCCCCCHHHHHHCCCCEECCCCCCCCCCCCCCHHHHCCEEEEEECCCCCCCCC
EVR
CCC
>Mature Secondary Structure 
SIDPTLLDDLEWRGLIAHSTDLDALREALAGGSVRFYVGFDPTAPSLHLGNLVQILTAK
CCCCCHHHCCCCCEEEECCCCHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHH
RLQEAGHTPYALVGGATGMIGDPKESGERTLNSLDTVKEWVERVRRQIEPFLSFEGANAA
HHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
TMVNNYDWTASLSTIDFLRDIGKHFPVNRMLSRDVVRSRLDAGISYTEFSYVLLQSMDFL
EEECCCCCEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
NLYRDHGVVLQFGGSDQWGNLTGGVELIRRAAGGKAHAFATPLITKADGTKYGKTEGGAL
HHHHCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCEEECCCEEECCCCCCCCCCCCCEE
WLDPEMMSPYAFYQFWLNVEDEKVGELLRVFTFLPRAEIEALEAEHAEKPFLRAGQRALA
EECHHHHCCCEEHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHH
EHVTTLVHGAEETERIRAASAALFGGGDLTTLSAATLGAALREAGSASVDGDEDLPTVVD
HHHHHHHHCCHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
LLVATGLAKSKGDARRTVTEGGAYLNNARVEDPDHVPAPEDLIDGTWLVLRRGKKNFAGV
HHHHHCCCCCCCCHHHHHHCCCCEECCCCCCCCCCCCCCHHHHCCEEEEEECCCCCCCCC
EVR
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]