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The map label for this gene is dus [H]

Identifier: 119716174

GI number: 119716174

Start: 2072359

End: 2073519

Strand: Direct

Name: dus [H]

Synonym: Noca_1942

Alternate gene names: 119716174

Gene position: 2072359-2073519 (Clockwise)

Preceding gene: 119716173

Following gene: 119716175

Centisome position: 41.56

GC content: 73.64

Gene sequence:

>1161_bases
ATGTCCTCGCCCACCTCACCGACCGGCAGTCCGGCCGGGGGGCTGGCCCTGGGGTCGCTGCGCGTCGACACCCCGGTGGT
GCTCGCGCCGATGGCCGGCATCACCAACGCGGCCTACCGCCGGCTGTGTGCCGAGCAGGGGGCCGGCCTCTACGTCTGCG
AGATGATCACCAGTCGGGGGCTGGTCGAGGGCGACCAGCACACCAAGGACATGCTGGTCTTCGACGAGCTCGAGACGATC
CGCTCCGTCCAGCTCTACGGCAGCGACCCGGCCTACGTCGGCAAGGCCGCCGAGATCCTCTGCGCGGAGTACGGCGTCGC
GCACATCGACCTCAACTTCGGCTGCCCGGTGCCCAAGGTGACCCGCAAGGGCGGCGGCGGCGCGCTGCCGTGGAAGCGCG
GGCTGCTCGCCGAGATCCTGGAGTCGGCCGTCGCCGCGGCCGCGCCGTACGACGTGCCGGTCACGATGAAGACCCGCAAG
GGCATCGACGAGGATCACCTCACCTACCTCGACGCCGGCCGGATCGCGCAGGAGTCCGGATGTGCGGCGATCGCGCTGCA
CGGCCGGACGGTCGCGCAGGCCTACTCCGGCGCGGCCGATTGGGACGCGATCGCGGCGCTGGTCGAGCACGTGGACATCC
CGGTGCTCGGCAACGGCGACGTCTGGGAGGCCGCGGACGCACTGCGGATGGTCGAGGAGACCGGCGTCGCGGGCGTCGTG
GTCGGCCGCGGCTGCCTGGGCCGGCCCTGGCTCTTCCGCGACCTCGCCGCCGCGTTCGGTGGCGAGGACGTCGCGACCCT
GCCGGCCCTGGGCGAGGTGGCCGCGATGATGCGCCGGCACGCCGAGCTGCTGTGCCAGCACCTGGGGGAGGAGCGCGGCT
GCAAGGAGTTCCGCAAGCACGTGACCTGGTACCTCAAGGGTTTCGGCGCGGGCGGCGAGATGCGGCGCTCGCTGGGCCTG
GTCGACAGTCTCGCGGCACTCGACCGGCTGCTGGCCGAGCTCGACCCGGACGAGCCGTTCCCGGAGCGCGAGCTGGGCGC
CCCGCGCGGGCGCCAGGGATCGCCGCGGGCCAAGGTCGCCCTGCCCGAGGGTTGGCTCGAGGACGCGGACGGTCGCGGCC
GGCACGTCCAGGAGGACGCGGACGAGACCACCGGCGGGTGA

Upstream 100 bases:

>100_bases
CCCAAGGCCGGCAAGGGCGGCACCCGGGCCGGCAAGGGCGGCACCCGCGGCGGCTGAGCCCGATTTGGGGCGCCGGACGG
CGGCCCCGGACAATGGCTGC

Downstream 100 bases:

>100_bases
CGAGGAACACGCCGCTCCTGCTGCCCCCCGATTCCGGAGACCGCGGGGCGTGTGGTTGACTCGGGCGGTCGTTGCCGATT
CGTGACCTTTCTCGGGTGTC

Product: nifR3 family TIM-barrel protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MSSPTSPTGSPAGGLALGSLRVDTPVVLAPMAGITNAAYRRLCAEQGAGLYVCEMITSRGLVEGDQHTKDMLVFDELETI
RSVQLYGSDPAYVGKAAEILCAEYGVAHIDLNFGCPVPKVTRKGGGGALPWKRGLLAEILESAVAAAAPYDVPVTMKTRK
GIDEDHLTYLDAGRIAQESGCAAIALHGRTVAQAYSGAADWDAIAALVEHVDIPVLGNGDVWEAADALRMVEETGVAGVV
VGRGCLGRPWLFRDLAAAFGGEDVATLPALGEVAAMMRRHAELLCQHLGEERGCKEFRKHVTWYLKGFGAGGEMRRSLGL
VDSLAALDRLLAELDPDEPFPERELGAPRGRQGSPRAKVALPEGWLEDADGRGRHVQEDADETTGG

Sequences:

>Translated_386_residues
MSSPTSPTGSPAGGLALGSLRVDTPVVLAPMAGITNAAYRRLCAEQGAGLYVCEMITSRGLVEGDQHTKDMLVFDELETI
RSVQLYGSDPAYVGKAAEILCAEYGVAHIDLNFGCPVPKVTRKGGGGALPWKRGLLAEILESAVAAAAPYDVPVTMKTRK
GIDEDHLTYLDAGRIAQESGCAAIALHGRTVAQAYSGAADWDAIAALVEHVDIPVLGNGDVWEAADALRMVEETGVAGVV
VGRGCLGRPWLFRDLAAAFGGEDVATLPALGEVAAMMRRHAELLCQHLGEERGCKEFRKHVTWYLKGFGAGGEMRRSLGL
VDSLAALDRLLAELDPDEPFPERELGAPRGRQGSPRAKVALPEGWLEDADGRGRHVQEDADETTGG
>Mature_385_residues
SSPTSPTGSPAGGLALGSLRVDTPVVLAPMAGITNAAYRRLCAEQGAGLYVCEMITSRGLVEGDQHTKDMLVFDELETIR
SVQLYGSDPAYVGKAAEILCAEYGVAHIDLNFGCPVPKVTRKGGGGALPWKRGLLAEILESAVAAAAPYDVPVTMKTRKG
IDEDHLTYLDAGRIAQESGCAAIALHGRTVAQAYSGAADWDAIAALVEHVDIPVLGNGDVWEAADALRMVEETGVAGVVV
GRGCLGRPWLFRDLAAAFGGEDVATLPALGEVAAMMRRHAELLCQHLGEERGCKEFRKHVTWYLKGFGAGGEMRRSLGLV
DSLAALDRLLAELDPDEPFPERELGAPRGRQGSPRAKVALPEGWLEDADGRGRHVQEDADETTGG

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788462, Length=232, Percent_Identity=34.4827586206897, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI239788483, Length=232, Percent_Identity=34.4827586206897, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI40807366, Length=230, Percent_Identity=33.0434782608696, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI31742496, Length=304, Percent_Identity=25.3289473684211, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI8923374, Length=254, Percent_Identity=28.3464566929134, Blast_Score=72, Evalue=8e-13,
Organism=Escherichia coli, GI1789660, Length=310, Percent_Identity=37.4193548387097, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1788462, Length=326, Percent_Identity=26.6871165644172, Blast_Score=91, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17543114, Length=245, Percent_Identity=31.4285714285714, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI25144369, Length=223, Percent_Identity=30.0448430493274, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17507177, Length=270, Percent_Identity=25.1851851851852, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323433, Length=315, Percent_Identity=26.6666666666667, Blast_Score=99, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6323437, Length=232, Percent_Identity=29.7413793103448, Blast_Score=89, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24585320, Length=268, Percent_Identity=30.5970149253731, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24580595, Length=303, Percent_Identity=29.7029702970297, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI19920448, Length=303, Percent_Identity=29.7029702970297, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI19921524, Length=229, Percent_Identity=29.6943231441048, Blast_Score=89, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 40864; Mature: 40733

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPTSPTGSPAGGLALGSLRVDTPVVLAPMAGITNAAYRRLCAEQGAGLYVCEMITSRG
CCCCCCCCCCCCCCEEECCEECCCCEEECCHHHHHHHHHHHHHHHCCCCEEEHHHHHHCC
LVEGDQHTKDMLVFDELETIRSVQLYGSDPAYVGKAAEILCAEYGVAHIDLNFGCPVPKV
CCCCCCCCHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCHH
TRKGGGGALPWKRGLLAEILESAVAAAAPYDVPVTMKTRKGIDEDHLTYLDAGRIAQESG
HCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEHHHCCCCHHHHEEECCCCHHHHCC
CAAIALHGRTVAQAYSGAADWDAIAALVEHVDIPVLGNGDVWEAADALRMVEETGVAGVV
CEEEEECCHHHHHHHCCCCCHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHCCCCEEE
VGRGCLGRPWLFRDLAAAFGGEDVATLPALGEVAAMMRRHAELLCQHLGEERGCKEFRKH
ECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHCCHHHHHHH
VTWYLKGFGAGGEMRRSLGLVDSLAALDRLLAELDPDEPFPERELGAPRGRQGSPRAKVA
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCCCCCEEE
LPEGWLEDADGRGRHVQEDADETTGG
CCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SSPTSPTGSPAGGLALGSLRVDTPVVLAPMAGITNAAYRRLCAEQGAGLYVCEMITSRG
CCCCCCCCCCCCCEEECCEECCCCEEECCHHHHHHHHHHHHHHHCCCCEEEHHHHHHCC
LVEGDQHTKDMLVFDELETIRSVQLYGSDPAYVGKAAEILCAEYGVAHIDLNFGCPVPKV
CCCCCCCCHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCHH
TRKGGGGALPWKRGLLAEILESAVAAAAPYDVPVTMKTRKGIDEDHLTYLDAGRIAQESG
HCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEHHHCCCCHHHHEEECCCCHHHHCC
CAAIALHGRTVAQAYSGAADWDAIAALVEHVDIPVLGNGDVWEAADALRMVEETGVAGVV
CEEEEECCHHHHHHHCCCCCHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHCCCCEEE
VGRGCLGRPWLFRDLAAAFGGEDVATLPALGEVAAMMRRHAELLCQHLGEERGCKEFRKH
ECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHCCHHHHHHH
VTWYLKGFGAGGEMRRSLGLVDSLAALDRLLAELDPDEPFPERELGAPRGRQGSPRAKVA
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCCCCCEEE
LPEGWLEDADGRGRHVQEDADETTGG
CCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]