Click here to switch to the map view.

The map label for this gene is leuA [H]

Identifier: 119716159

GI number: 119716159

Start: 2054465

End: 2056207

Strand: Direct

Name: leuA [H]

Synonym: Noca_1927

Alternate gene names: 119716159

Gene position: 2054465-2056207 (Clockwise)

Preceding gene: 119716158

Following gene: 119716161

Centisome position: 41.21

GC content: 69.19

Gene sequence:

>1743_bases
GTGCGCCGGCCCCGCACCGACCCGAGCGAGGACATCATGACCATTCCCCAGCAGCAGCCCAGCGGTATGCCGTTCCGGCG
CTACCGCCCGTTCCCCCCGATCGACCTGCCCGACCGCAGCTGGCCGCAGCGCACGATCACCCAGGCACCCCGGTGGCTCT
CCACCGACCTGCGCGACGGCAACCAGGCACTGATCGACCCGATGTCACCCGCCCGCAAGCTGAAGATGTTCGACCTGCTC
GTGCGGATGGGCTACAAGGAGATCGAGGTCGGCTTCCCGTCGGCCAGCGAGACCGACTTCAGCTTCGTGCGCCAGCTCAT
CGAGAGCGACCACATCCCCGACGACGTCACGATCTCGGTGCTGACCCAGGCCCGCGAGGACCTGATCGAGCGCAGCGTGC
AGTCGCTGATCGGCGTACCCCGCGCGAACATCCACCTCTACAACGCGCTCGCGCCGCTGTTCCGCCGTGTGGTGTTCCGG
GCCAGCAAGGACGAGGTCAAGGACATCGCCGTGCGCGGCACCCAGCTGGTGATGAAGCACGTCGAGAACAACCTCGACAC
GACCGTCATCGGCTACGAGTACAGCCCGGAGATCTTCACCAGCACCGAGCTGCCGTTCTCGGTGGAGGTCTGCGAGGCGG
TCTCGGACGTCTGGCAGCCCGACGACGGCCGCGAGATCATCCTGAACCTGCCGGCGACCGTCGAGGTCGCGACCCCGAAC
GTGTACGCCGACCAGATCGAGTGGTTCTCCCGCCAGCTGACCCGGCGCGCGAACACGGTGATCTCGCTGCACCCGCACAA
CGACCGCGGCACCGCGGTCGCGGCGACCGAGCTGGCCGTGATGGCCGGCGCGGACCGGGTCGAGGGCTGCCTGTTCGGGC
ACGGCGAGCGCACCGGCAACGTCGACCTGGTCACGCTGGGGCTGAACCTGTTCAGCCAGGGCGTCGACCCGCAGGTCGAC
TTCTCCGACATCGACGAGATCCGGCGCACGGTCGAGTACTGCACCCAGCTGCCCGTCCACCCGCGACACCCGTACGCCGG
CGACCTGGTCTACACGGCGTTCTCCGGCTCCCACCAGGACGCGATCAAGAAGGGCCTGGAGGACCTGGACCGGCAGGCCG
CCGAGCAGGGCGTGCCGGTGGGGGAGCTGCCCTGGGAGGCGCCGTACCTGCCGATCGACCCGAAGGACGTCGGCCGCACC
TACGAGGCCGTGATCCGGGTGAACAGCCAGTCCGGCAAGGGCGGCGTGGCCTACATCCTGAAGGCCGAGCACAAGCTCGA
CCTGCCCCGCCGCGCGCAGATCGAGTTCAGCCGGGTGGTCCAGGAGCGCACCGACGCCGAGGGCGGCGAGATGTCGCCCG
ACGAGATCTGGTCGGTGTTCTCAGCCGAGTACCTCGACCGTCGGACGCCCCTCGAGCTCAACTCCGTGCACACCTCGTCG
GCGGCGGGGGAGAAGGACGCGCTCGACGTGAACGTCTACGTCCACGGCGAGCTGATGTCGCTGCACGGGGTCGGCAACGG
CCCGATCGCGGCCTTCGTCAGCGCGATCAACGAGCTCCCGAACGACTGGGACGTCCGGGTCCTCGACTACGCCGAGCACG
CACTCTCCGCCGGCGGCGACGCGCTCGCGGCGGCGTACGTCGAGTGCCTGGTCCGCGACCAGGTCTACTGGGGCGTCGGC
GTCGACGCCAACATCGTCACCGCGTCGCTGAAGGCCGTGATCAGCGCCGTCAACAGGGCTTGA

Upstream 100 bases:

>100_bases
GAGTGGCCCCGTCATGACGCGCAGCCTCCTCCTTCGCTGCCGCAGCGAGGTCTGAGCCCACCAGGGCAGAGTCCGGACCC
CCTCGCTGCGGAGTGACTGC

Downstream 100 bases:

>100_bases
CGGGCGGGCCTAACGGGCCAGGGGCAGTCGGACGGTGAAGGTGGTGCCCTGCAGGTGGGCGGAGTCGACGTCGATCCGCC
CACCGTGGGCAGCGACCGTG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 580; Mature: 580

Protein sequence:

>580_residues
MRRPRTDPSEDIMTIPQQQPSGMPFRRYRPFPPIDLPDRSWPQRTITQAPRWLSTDLRDGNQALIDPMSPARKLKMFDLL
VRMGYKEIEVGFPSASETDFSFVRQLIESDHIPDDVTISVLTQAREDLIERSVQSLIGVPRANIHLYNALAPLFRRVVFR
ASKDEVKDIAVRGTQLVMKHVENNLDTTVIGYEYSPEIFTSTELPFSVEVCEAVSDVWQPDDGREIILNLPATVEVATPN
VYADQIEWFSRQLTRRANTVISLHPHNDRGTAVAATELAVMAGADRVEGCLFGHGERTGNVDLVTLGLNLFSQGVDPQVD
FSDIDEIRRTVEYCTQLPVHPRHPYAGDLVYTAFSGSHQDAIKKGLEDLDRQAAEQGVPVGELPWEAPYLPIDPKDVGRT
YEAVIRVNSQSGKGGVAYILKAEHKLDLPRRAQIEFSRVVQERTDAEGGEMSPDEIWSVFSAEYLDRRTPLELNSVHTSS
AAGEKDALDVNVYVHGELMSLHGVGNGPIAAFVSAINELPNDWDVRVLDYAEHALSAGGDALAAAYVECLVRDQVYWGVG
VDANIVTASLKAVISAVNRA

Sequences:

>Translated_580_residues
MRRPRTDPSEDIMTIPQQQPSGMPFRRYRPFPPIDLPDRSWPQRTITQAPRWLSTDLRDGNQALIDPMSPARKLKMFDLL
VRMGYKEIEVGFPSASETDFSFVRQLIESDHIPDDVTISVLTQAREDLIERSVQSLIGVPRANIHLYNALAPLFRRVVFR
ASKDEVKDIAVRGTQLVMKHVENNLDTTVIGYEYSPEIFTSTELPFSVEVCEAVSDVWQPDDGREIILNLPATVEVATPN
VYADQIEWFSRQLTRRANTVISLHPHNDRGTAVAATELAVMAGADRVEGCLFGHGERTGNVDLVTLGLNLFSQGVDPQVD
FSDIDEIRRTVEYCTQLPVHPRHPYAGDLVYTAFSGSHQDAIKKGLEDLDRQAAEQGVPVGELPWEAPYLPIDPKDVGRT
YEAVIRVNSQSGKGGVAYILKAEHKLDLPRRAQIEFSRVVQERTDAEGGEMSPDEIWSVFSAEYLDRRTPLELNSVHTSS
AAGEKDALDVNVYVHGELMSLHGVGNGPIAAFVSAINELPNDWDVRVLDYAEHALSAGGDALAAAYVECLVRDQVYWGVG
VDANIVTASLKAVISAVNRA
>Mature_580_residues
MRRPRTDPSEDIMTIPQQQPSGMPFRRYRPFPPIDLPDRSWPQRTITQAPRWLSTDLRDGNQALIDPMSPARKLKMFDLL
VRMGYKEIEVGFPSASETDFSFVRQLIESDHIPDDVTISVLTQAREDLIERSVQSLIGVPRANIHLYNALAPLFRRVVFR
ASKDEVKDIAVRGTQLVMKHVENNLDTTVIGYEYSPEIFTSTELPFSVEVCEAVSDVWQPDDGREIILNLPATVEVATPN
VYADQIEWFSRQLTRRANTVISLHPHNDRGTAVAATELAVMAGADRVEGCLFGHGERTGNVDLVTLGLNLFSQGVDPQVD
FSDIDEIRRTVEYCTQLPVHPRHPYAGDLVYTAFSGSHQDAIKKGLEDLDRQAAEQGVPVGELPWEAPYLPIDPKDVGRT
YEAVIRVNSQSGKGGVAYILKAEHKLDLPRRAQIEFSRVVQERTDAEGGEMSPDEIWSVFSAEYLDRRTPLELNSVHTSS
AAGEKDALDVNVYVHGELMSLHGVGNGPIAAFVSAINELPNDWDVRVLDYAEHALSAGGDALAAAYVECLVRDQVYWGVG
VDANIVTASLKAVISAVNRA

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=543, Percent_Identity=24.8618784530387, Blast_Score=126, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6324225, Length=568, Percent_Identity=48.943661971831, Blast_Score=546, Evalue=1e-156,
Organism=Saccharomyces cerevisiae, GI6324682, Length=567, Percent_Identity=48.6772486772487, Blast_Score=540, Evalue=1e-154,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 64405; Mature: 64405

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRPRTDPSEDIMTIPQQQPSGMPFRRYRPFPPIDLPDRSWPQRTITQAPRWLSTDLRDG
CCCCCCCCCHHHEECCCCCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHCCCHHHHHCCCC
NQALIDPMSPARKLKMFDLLVRMGYKEIEVGFPSASETDFSFVRQLIESDHIPDDVTISV
CCEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHCCCCCCCHHHHH
LTQAREDLIERSVQSLIGVPRANIHLYNALAPLFRRVVFRASKDEVKDIAVRGTQLVMKH
HHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
VENNLDTTVIGYEYSPEIFTSTELPFSVEVCEAVSDVWQPDDGREIILNLPATVEVATPN
HHCCCCCEEEEEEECCCEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCEEEEECCC
VYADQIEWFSRQLTRRANTVISLHPHNDRGTAVAATELAVMAGADRVEGCLFGHGERTGN
HHHHHHHHHHHHHHHHHCCEEEEECCCCCCCEEEHHHHHHHCCCHHHCCEEEECCCCCCC
VDLVTLGLNLFSQGVDPQVDFSDIDEIRRTVEYCTQLPVHPRHPYAGDLVYTAFSGSHQD
EEEEEECHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCHH
AIKKGLEDLDRQAAEQGVPVGELPWEAPYLPIDPKDVGRTYEAVIRVNSQSGKGGVAYIL
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCEEEEEEECCCCCCCCEEEEE
KAEHKLDLPRRAQIEFSRVVQERTDAEGGEMSPDEIWSVFSAEYLDRRTPLELNSVHTSS
EECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCCC
AAGEKDALDVNVYVHGELMSLHGVGNGPIAAFVSAINELPNDWDVRVLDYAEHALSAGGD
CCCCCCCEEEEEEEEEEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEHHHHHHHHHCCCH
ALAAAYVECLVRDQVYWGVGVDANIVTASLKAVISAVNRA
HHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRRPRTDPSEDIMTIPQQQPSGMPFRRYRPFPPIDLPDRSWPQRTITQAPRWLSTDLRDG
CCCCCCCCCHHHEECCCCCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHCCCHHHHHCCCC
NQALIDPMSPARKLKMFDLLVRMGYKEIEVGFPSASETDFSFVRQLIESDHIPDDVTISV
CCEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHCCCCCCCHHHHH
LTQAREDLIERSVQSLIGVPRANIHLYNALAPLFRRVVFRASKDEVKDIAVRGTQLVMKH
HHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
VENNLDTTVIGYEYSPEIFTSTELPFSVEVCEAVSDVWQPDDGREIILNLPATVEVATPN
HHCCCCCEEEEEEECCCEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCEEEEECCC
VYADQIEWFSRQLTRRANTVISLHPHNDRGTAVAATELAVMAGADRVEGCLFGHGERTGN
HHHHHHHHHHHHHHHHHCCEEEEECCCCCCCEEEHHHHHHHCCCHHHCCEEEECCCCCCC
VDLVTLGLNLFSQGVDPQVDFSDIDEIRRTVEYCTQLPVHPRHPYAGDLVYTAFSGSHQD
EEEEEECHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCHH
AIKKGLEDLDRQAAEQGVPVGELPWEAPYLPIDPKDVGRTYEAVIRVNSQSGKGGVAYIL
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCEEEEEEECCCCCCCCEEEEE
KAEHKLDLPRRAQIEFSRVVQERTDAEGGEMSPDEIWSVFSAEYLDRRTPLELNSVHTSS
EECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCCC
AAGEKDALDVNVYVHGELMSLHGVGNGPIAAFVSAINELPNDWDVRVLDYAEHALSAGGD
CCCCCCCEEEEEEEEEEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEHHHHHHHHHCCCH
ALAAAYVECLVRDQVYWGVGVDANIVTASLKAVISAVNRA
HHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA