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The map label for this gene is mhpE [C]

Identifier: 119715884

GI number: 119715884

Start: 1751756

End: 1752775

Strand: Reverse

Name: mhpE [C]

Synonym: Noca_1649

Alternate gene names: 119715884

Gene position: 1752775-1751756 (Counterclockwise)

Preceding gene: 119715885

Following gene: 119715881

Centisome position: 35.15

GC content: 71.57

Gene sequence:

>1020_bases
GTGAACGCACACCAGATCTTCGTCCAGGACGTCACGCTGCGCGACGGGATGCACGCCGTGCGGCACCGGATCGGGCTCGA
CGACGTGCGCCGCATCGTCGCCGCGCTCGACGCCGCCGGCGTCGACGCCATCGAGGTCGCCCACGGCGACGGCCTCGCCG
GCTCCTCGGTGAACTACGGACCCGGATCCCACACCGACTGGGAGTGGATCGAGGCCGCGGCCGACGTGCTCGAGCGGGCC
CGCCTGACCACGCTGCTGCTACCCGGGGTCGGCACCATCCACGAGCTCAAGACCGCCTACGACCTCGGGGTCCGCTCGGT
CCGGGTCGCCACGCACTGCACGGAGGCCGACATCTCCGCCCAGCACATCACCGCGGCCCGGGAGATCGGCATGGACGTCT
CCGGCTTCCTGATGCTCTCCCACATGGCGCCGCCGGCGGAGCTCGCCAAGCAGGCCCTGCTCATGGAGTCCTACGGTGCG
CACTGCGTCTACGTCACCGACTCCGGCGGCCGGCTCACGATGAACGACGTCCGCGACCGGGTCGCGGCGTACCGAGACGT
CCTCGACCCTGCCACCGAGATCGGCATCCACGCCCACGAGAACCTCTCGCTCTCGGTCGCCAACTCCGTCGTGGCCGTCG
AGACCGGTGCGGTGCGGGTCGACGCCTCCCTCGCCGGGCACGGTGCCGGCGCCGGCAACTGTCCGATCGAGGCGTTCGTC
GCGGTGGCGAACCTCTCCGGCTTCGAGCACGGCTGCGACCTGTTCGCGCTGCAGGACGCCGCCGACGACCTGGTCCGCCC
GTTGCAGGACCGCCCGGTCCGGGTGGACCGCGAGACCCTCACCCTCGGCTACGCCGGGGTCTACTCCTCGTTCCTACGGC
ACGCCGAGCGGGCCGCCGATCAGTACGGCGTCGACGTGCGCGAGCTGCTGATGGAGTGCGGCCGGCGCGGCCTGGTCGGT
GGCCAGGAGGACATGATCATCGACATCGCGCTCGATCAGGTCGGCGCCGTCGCCAGCTGA

Upstream 100 bases:

>100_bases
GCGCCGCGCACTACCTCCCGGCGTACGCCGGGAACCTCGACATCATGACCTCCGCCGCGCTCCAGGTGGCCGAGCGGATC
GCTGCAGGGAAGGCCGACCG

Downstream 100 bases:

>100_bases
CCGGTGCCCGCACCGGGGGGAGCCGGTCACTCGGGGGAGCCCCGCCACCCGGGCTCCGAAGGAGGCAGGCTCCACTGAGT
GCATCGCCCTACCTGCGGAG

Product: 4-hydroxy-2-ketovalerate aldolase

Products: NA

Alternate protein names: HOA 1; 4-hydroxy-2-keto-pentanoic acid aldolase 1; 4-hydroxy-2-oxopentanoate aldolase 1

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MNAHQIFVQDVTLRDGMHAVRHRIGLDDVRRIVAALDAAGVDAIEVAHGDGLAGSSVNYGPGSHTDWEWIEAAADVLERA
RLTTLLLPGVGTIHELKTAYDLGVRSVRVATHCTEADISAQHITAAREIGMDVSGFLMLSHMAPPAELAKQALLMESYGA
HCVYVTDSGGRLTMNDVRDRVAAYRDVLDPATEIGIHAHENLSLSVANSVVAVETGAVRVDASLAGHGAGAGNCPIEAFV
AVANLSGFEHGCDLFALQDAADDLVRPLQDRPVRVDRETLTLGYAGVYSSFLRHAERAADQYGVDVRELLMECGRRGLVG
GQEDMIIDIALDQVGAVAS

Sequences:

>Translated_339_residues
MNAHQIFVQDVTLRDGMHAVRHRIGLDDVRRIVAALDAAGVDAIEVAHGDGLAGSSVNYGPGSHTDWEWIEAAADVLERA
RLTTLLLPGVGTIHELKTAYDLGVRSVRVATHCTEADISAQHITAAREIGMDVSGFLMLSHMAPPAELAKQALLMESYGA
HCVYVTDSGGRLTMNDVRDRVAAYRDVLDPATEIGIHAHENLSLSVANSVVAVETGAVRVDASLAGHGAGAGNCPIEAFV
AVANLSGFEHGCDLFALQDAADDLVRPLQDRPVRVDRETLTLGYAGVYSSFLRHAERAADQYGVDVRELLMECGRRGLVG
GQEDMIIDIALDQVGAVAS
>Mature_339_residues
MNAHQIFVQDVTLRDGMHAVRHRIGLDDVRRIVAALDAAGVDAIEVAHGDGLAGSSVNYGPGSHTDWEWIEAAADVLERA
RLTTLLLPGVGTIHELKTAYDLGVRSVRVATHCTEADISAQHITAAREIGMDVSGFLMLSHMAPPAELAKQALLMESYGA
HCVYVTDSGGRLTMNDVRDRVAAYRDVLDPATEIGIHAHENLSLSVANSVVAVETGAVRVDASLAGHGAGAGNCPIEAFV
AVANLSGFEHGCDLFALQDAADDLVRPLQDRPVRVDRETLTLGYAGVYSSFLRHAERAADQYGVDVRELLMECGRRGLVG
GQEDMIIDIALDQVGAVAS

Specific function: Catalyzes the retro-aldol cleavage of 4-hydroxy-2- oxopentanoate to pyruvate and acetaldehyde. Is involved in the meta-cleavage pathway for the degradation of aromatic compounds

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 4-hydroxy-2-oxovalerate aldolase family

Homologues:

Organism=Escherichia coli, GI1786548, Length=332, Percent_Identity=66.566265060241, Blast_Score=444, Evalue=1e-126,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HOA1_NOCSJ (A1SH77)

Other databases:

- EMBL:   CP000509
- RefSeq:   YP_922849.1
- ProteinModelPortal:   A1SH77
- SMR:   A1SH77
- STRING:   A1SH77
- GeneID:   4600028
- GenomeReviews:   CP000509_GR
- KEGG:   nca:Noca_1649
- eggNOG:   COG0119
- HOGENOM:   HBG300122
- OMA:   TTRLFIQ
- ProtClustDB:   PRK08195
- BioCyc:   NSP35761:NOCA_1649-MONOMER
- HAMAP:   MF_01656
- InterPro:   IPR017629
- InterPro:   IPR013785
- InterPro:   IPR012425
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- ProDom:   PD005364
- TIGRFAMs:   TIGR03217

Pfam domain/function: PF07836 DmpG_comm; PF00682 HMGL-like

EC number: =4.1.3.39

Molecular weight: Translated: 36092; Mature: 36092

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS50991 PYR_CT

Important sites: BINDING 168-168 BINDING 197-197 BINDING 288-288

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAHQIFVQDVTLRDGMHAVRHRIGLDDVRRIVAALDAAGVDAIEVAHGDGLAGSSVNYG
CCCCEEEEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCC
PGSHTDWEWIEAAADVLERARLTTLLLPGVGTIHELKTAYDLGVRSVRVATHCTEADISA
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEHHHHCCCCCCCH
QHITAAREIGMDVSGFLMLSHMAPPAELAKQALLMESYGAHCVYVTDSGGRLTMNDVRDR
HHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCCEEEHHHHHHH
VAAYRDVLDPATEIGIHAHENLSLSVANSVVAVETGAVRVDASLAGHGAGAGNCPIEAFV
HHHHHHHHCHHHHHCCCCCCCCEEEECCCEEEEECCCEEEECCCCCCCCCCCCCCHHHHH
AVANLSGFEHGCDLFALQDAADDLVRPLQDRPVRVDRETLTLGYAGVYSSFLRHAERAAD
HHHHCCCCCCCCCEEEEHHHHHHHHHHHHCCCCEECCHHEEEEHHHHHHHHHHHHHHHHH
QYGVDVRELLMECGRRGLVGGQEDMIIDIALDQVGAVAS
HCCCCHHHHHHHCCCCCCCCCCCCEEEEEEHHHHCCCCC
>Mature Secondary Structure
MNAHQIFVQDVTLRDGMHAVRHRIGLDDVRRIVAALDAAGVDAIEVAHGDGLAGSSVNYG
CCCCEEEEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCC
PGSHTDWEWIEAAADVLERARLTTLLLPGVGTIHELKTAYDLGVRSVRVATHCTEADISA
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEHHHHCCCCCCCH
QHITAAREIGMDVSGFLMLSHMAPPAELAKQALLMESYGAHCVYVTDSGGRLTMNDVRDR
HHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCCEEEHHHHHHH
VAAYRDVLDPATEIGIHAHENLSLSVANSVVAVETGAVRVDASLAGHGAGAGNCPIEAFV
HHHHHHHHCHHHHHCCCCCCCCEEEECCCEEEEECCCEEEECCCCCCCCCCCCCCHHHHH
AVANLSGFEHGCDLFALQDAADDLVRPLQDRPVRVDRETLTLGYAGVYSSFLRHAERAAD
HHHHCCCCCCCCCEEEEHHHHHHHHHHHHCCCCEECCHHEEEEHHHHHHHHHHHHHHHHH
QYGVDVRELLMECGRRGLVGGQEDMIIDIALDQVGAVAS
HCCCCHHHHHHHCCCCCCCCCCCCEEEEEEHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA