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The map label for this gene is pyrB [H]

Identifier: 119715866

GI number: 119715866

Start: 1731439

End: 1732392

Strand: Direct

Name: pyrB [H]

Synonym: Noca_1631

Alternate gene names: 119715866

Gene position: 1731439-1732392 (Clockwise)

Preceding gene: 119715865

Following gene: 119715867

Centisome position: 34.73

GC content: 68.24

Gene sequence:

>954_bases
ATGAGGAGCTTCCTGGGCCGCGACATCCTGTCCCTGAAGGACGTCGAGCGCGACGACTACTTCCGGATCTTCCAGGTGGC
CGAGGAGCTGCGCCCTCACGCGGAGAACCGTCGCAACGGGGATCTCCTCCAGCACAAGACGCTGCTGACCGCGTTCTACC
AGCCCAGCACCCGCACCCGGCTCTCGACGGAGGCCGCGATGCACCGCCTCGGCGGTCACGTCCTCGGCTTCTCCGACGCC
AAGATGACCCGCGCCGGCGACTTCTACCAGGAGTCGATGAAGGACACCGTGAAGATGCTGGAGTTCTACGGCGACGTGAT
CGCCATGCGCTCCCACGTCCAGGGCGCCCCGCACGAAGCCGCGCGCTGGGCCTCGGTCCCGGTGATCAACTGCGGTGACG
GCTGGGGCGAGCACCCGACCCAGGTGCTCACCGACCTCTACACGATCTGGCGCGACAAGGGCAGCCTCGACGGCATCAAG
ATGCTCCTTGTCGGCGACATGCGGATGCGCACCATGCACTCGATCCTGCTCGCGTCCTCGCAGTTCGACATGGAGACGAT
GGTCGTCTCCCCGCCGGAGCTGGCCCTGCTCGAGGACTTCAAGGGCGAGCTCGCCGAGCGCAGCGTGAACTACCGCGAGC
TCGAGTCGGTCGAGCAGGGCATCGCCGACGCCGACGTGATCTACATGGAGCCGGTGGTCCAGGCCGACTACACGCAGTCC
CGCGTCGACCCCACCGTCGAGAAGGGCACCACGCCGCCGGCGTACCAGGTCACCCGGCGGCTGCTGCACGAGAAGGCCAA
GCAGGACTCGATCGTCCTGCACTCGCTGCCCCGGATGGACGAGCTCACTCCCGACGTGGACGCCACCCGGCACGCCCGCT
ACTGGGCCGAGGCCGCCAACGGCGTCTCGGTCCGGATGGCGCTGCTGGCGCTGGTCCTCGGCGCGGTCGAGTAG

Upstream 100 bases:

>100_bases
GTACGTCGAGGCGGGCGCCACGTGCCCCGTCCTCTACCCGCTCGGCGAGGACGTCCGACTGATGATCGACACCTTCGGTG
TCTGAAGAGGAGAACGCACG

Downstream 100 bases:

>100_bases
CCGGGGGAGGGGGACCGATGCAGACGCACCTGCGCGGCCGGGACGTCATCGACTTCCAGGAGTGGACCCGCGACGAGATC
GAGACCGTGCTCGACGTCGC

Product: aspartate carbamoyltransferase

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MRSFLGRDILSLKDVERDDYFRIFQVAEELRPHAENRRNGDLLQHKTLLTAFYQPSTRTRLSTEAAMHRLGGHVLGFSDA
KMTRAGDFYQESMKDTVKMLEFYGDVIAMRSHVQGAPHEAARWASVPVINCGDGWGEHPTQVLTDLYTIWRDKGSLDGIK
MLLVGDMRMRTMHSILLASSQFDMETMVVSPPELALLEDFKGELAERSVNYRELESVEQGIADADVIYMEPVVQADYTQS
RVDPTVEKGTTPPAYQVTRRLLHEKAKQDSIVLHSLPRMDELTPDVDATRHARYWAEAANGVSVRMALLALVLGAVE

Sequences:

>Translated_317_residues
MRSFLGRDILSLKDVERDDYFRIFQVAEELRPHAENRRNGDLLQHKTLLTAFYQPSTRTRLSTEAAMHRLGGHVLGFSDA
KMTRAGDFYQESMKDTVKMLEFYGDVIAMRSHVQGAPHEAARWASVPVINCGDGWGEHPTQVLTDLYTIWRDKGSLDGIK
MLLVGDMRMRTMHSILLASSQFDMETMVVSPPELALLEDFKGELAERSVNYRELESVEQGIADADVIYMEPVVQADYTQS
RVDPTVEKGTTPPAYQVTRRLLHEKAKQDSIVLHSLPRMDELTPDVDATRHARYWAEAANGVSVRMALLALVLGAVE
>Mature_317_residues
MRSFLGRDILSLKDVERDDYFRIFQVAEELRPHAENRRNGDLLQHKTLLTAFYQPSTRTRLSTEAAMHRLGGHVLGFSDA
KMTRAGDFYQESMKDTVKMLEFYGDVIAMRSHVQGAPHEAARWASVPVINCGDGWGEHPTQVLTDLYTIWRDKGSLDGIK
MLLVGDMRMRTMHSILLASSQFDMETMVVSPPELALLEDFKGELAERSVNYRELESVEQGIADADVIYMEPVVQADYTQS
RVDPTVEKGTTPPAYQVTRRLLHEKAKQDSIVLHSLPRMDELTPDVDATRHARYWAEAANGVSVRMALLALVLGAVE

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=315, Percent_Identity=36.5079365079365, Blast_Score=202, Evalue=4e-52,
Organism=Homo sapiens, GI38788445, Length=189, Percent_Identity=32.2751322751323, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI2367364, Length=311, Percent_Identity=38.2636655948553, Blast_Score=201, Evalue=4e-53,
Organism=Escherichia coli, GI1790703, Length=285, Percent_Identity=27.719298245614, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1786469, Length=286, Percent_Identity=27.6223776223776, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI193204318, Length=330, Percent_Identity=33.9393939393939, Blast_Score=167, Evalue=6e-42,
Organism=Saccharomyces cerevisiae, GI6322331, Length=316, Percent_Identity=36.0759493670886, Blast_Score=184, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24642586, Length=316, Percent_Identity=33.5443037974684, Blast_Score=166, Evalue=1e-41,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 35809; Mature: 35809

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSFLGRDILSLKDVERDDYFRIFQVAEELRPHAENRRNGDLLQHKTLLTAFYQPSTRTR
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCHH
LSTEAAMHRLGGHVLGFSDAKMTRAGDFYQESMKDTVKMLEFYGDVIAMRSHVQGAPHEA
HHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
ARWASVPVINCGDGWGEHPTQVLTDLYTIWRDKGSLDGIKMLLVGDMRMRTMHSILLASS
HHHCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEHCCHHHHHHHHHHHHHCC
QFDMETMVVSPPELALLEDFKGELAERSVNYRELESVEQGIADADVIYMEPVVQADYTQS
CCCCEEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEECCHHHCCCHHH
RVDPTVEKGTTPPAYQVTRRLLHEKAKQDSIVLHSLPRMDELTPDVDATRHARYWAEAAN
HCCCCHHCCCCCHHHHHHHHHHHHHHHCCCCHHHCCCCHHCCCCCCCHHHHHHHHHHHCC
GVSVRMALLALVLGAVE
CHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRSFLGRDILSLKDVERDDYFRIFQVAEELRPHAENRRNGDLLQHKTLLTAFYQPSTRTR
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCHH
LSTEAAMHRLGGHVLGFSDAKMTRAGDFYQESMKDTVKMLEFYGDVIAMRSHVQGAPHEA
HHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
ARWASVPVINCGDGWGEHPTQVLTDLYTIWRDKGSLDGIKMLLVGDMRMRTMHSILLASS
HHHCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEHCCHHHHHHHHHHHHHCC
QFDMETMVVSPPELALLEDFKGELAERSVNYRELESVEQGIADADVIYMEPVVQADYTQS
CCCCEEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEECCHHHCCCHHH
RVDPTVEKGTTPPAYQVTRRLLHEKAKQDSIVLHSLPRMDELTPDVDATRHARYWAEAAN
HCCCCHHCCCCCHHHHHHHHHHHHHHHCCCCHHHCCCCHHCCCCCCCHHHHHHHHHHHCC
GVSVRMALLALVLGAVE
CHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11121031 [H]