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The map label for this gene is rph

Identifier: 119715559

GI number: 119715559

Start: 1395035

End: 1395763

Strand: Direct

Name: rph

Synonym: Noca_1323

Alternate gene names: 119715559

Gene position: 1395035-1395763 (Clockwise)

Preceding gene: 119715558

Following gene: 119715560

Centisome position: 27.98

GC content: 72.98

Gene sequence:

>729_bases
ATGAGTGCAGCACCACGCGAGGACGGCCGGGCCGACGACGAGCTCCGTCCGATCACGATCACCCGGCACTGGCTGGACCA
CGCCGCCGGGTCGGTCCTGGTGGAGTTCGGCAGGACCAGGGTCCTCTGCGCGGCCTCGGCCGCCGAGGGGGTGCCGCGGT
GGCGCAAGGGCTCCGGCCTCGGCTGGGTCACCGCCGAGTACGCCATGCTCCCCGCCTCCACCAACACCCGGTCCGACCGC
GAGTCCGTCAAGGGCCGGATCGGCGGCCGCACCCACGAGATCAGCCGCCTGATCGGCCGGTCGCTGCGCGCGATCATCGA
CTACAAGGCGCTCGGGGAGAACACCATCCAGCTCGACTGCGACGTGCTCCAGGCCGACGGCGGTACTCGCACGGCGGCCA
TCACCGGCGCCTACGTCGCGCTCGCCGACGCGGTCGAGCACCTGCGCTCGACCGGGGCCCTCACCGGCGAGCCGCTCACC
GGCTCCGTGGCCGCGGTCAGCGTCGGGATCATCGACGGCGTGCCCCGCCTCGACCTGCCCTACGTCGAGGACGTGCGGGC
CGAGACCGACATGAACGTCGTGATGACCGGCTCCGGGAAGTTCGTCGAGGTGCAGGGCACTGCGGAGGGGGCCGCCTTCG
ACCGCGCCGAGCTCGACGCGCTGCTGGCCCTGGCGGAGAAGGGGTGCGCCGACCTCACCCGTCTCCAGCAGGAGGCGCTG
AGCCGGTGA

Upstream 100 bases:

>100_bases
TGGTCGTGTCCGGGATCGTCTGGCTCGGCCTGCCCCTCGGGTTCGGCACCTGGCGGGTGCTGCGCGCGGAGGTGAAGTAG
CCCCGGCTAGGGTCCTGGGC

Downstream 100 bases:

>100_bases
CCGCTCCCGTCCGGCGGGTGTTCCTGGCCTCGCGCAACCGCAAGAAGATCGCGGAGATGGAGCGGATCCTGCGCGAGCAC
GTCCCCGGCATCGTGGTCGT

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase

Number of amino acids: Translated: 242; Mature: 241

Protein sequence:

>242_residues
MSAAPREDGRADDELRPITITRHWLDHAAGSVLVEFGRTRVLCAASAAEGVPRWRKGSGLGWVTAEYAMLPASTNTRSDR
ESVKGRIGGRTHEISRLIGRSLRAIIDYKALGENTIQLDCDVLQADGGTRTAAITGAYVALADAVEHLRSTGALTGEPLT
GSVAAVSVGIIDGVPRLDLPYVEDVRAETDMNVVMTGSGKFVEVQGTAEGAAFDRAELDALLALAEKGCADLTRLQQEAL
SR

Sequences:

>Translated_242_residues
MSAAPREDGRADDELRPITITRHWLDHAAGSVLVEFGRTRVLCAASAAEGVPRWRKGSGLGWVTAEYAMLPASTNTRSDR
ESVKGRIGGRTHEISRLIGRSLRAIIDYKALGENTIQLDCDVLQADGGTRTAAITGAYVALADAVEHLRSTGALTGEPLT
GSVAAVSVGIIDGVPRLDLPYVEDVRAETDMNVVMTGSGKFVEVQGTAEGAAFDRAELDALLALAEKGCADLTRLQQEAL
SR
>Mature_241_residues
SAAPREDGRADDELRPITITRHWLDHAAGSVLVEFGRTRVLCAASAAEGVPRWRKGSGLGWVTAEYAMLPASTNTRSDRE
SVKGRIGGRTHEISRLIGRSLRAIIDYKALGENTIQLDCDVLQADGGTRTAAITGAYVALADAVEHLRSTGALTGEPLTG
SVAAVSVGIIDGVPRLDLPYVEDVRAETDMNVVMTGSGKFVEVQGTAEGAAFDRAELDALLALAEKGCADLTRLQQEALS
R

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family

Homologues:

Organism=Escherichia coli, GI157672248, Length=208, Percent_Identity=64.4230769230769, Blast_Score=270, Evalue=6e-74,
Organism=Caenorhabditis elegans, GI71981632, Length=192, Percent_Identity=31.7708333333333, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNPH_NOCSJ (A1SGA2)

Other databases:

- EMBL:   CP000509
- RefSeq:   YP_922524.1
- ProteinModelPortal:   A1SGA2
- SMR:   A1SGA2
- STRING:   A1SGA2
- GeneID:   4598561
- GenomeReviews:   CP000509_GR
- KEGG:   nca:Noca_1323
- eggNOG:   COG0689
- HOGENOM:   HBG737187
- OMA:   MLPRATG
- PhylomeDB:   A1SGA2
- ProtClustDB:   PRK00173
- BioCyc:   NSP35761:NOCA_1323-MONOMER
- HAMAP:   MF_00564
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336
- TIGRFAMs:   TIGR01966

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.56

Molecular weight: Translated: 25671; Mature: 25540

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAAPREDGRADDELRPITITRHWLDHAAGSVLVEFGRTRVLCAASAAEGVPRWRKGSGL
CCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCEEEEECHHHHCCCCCCCCCCC
GWVTAEYAMLPASTNTRSDRESVKGRIGGRTHEISRLIGRSLRAIIDYKALGENTIQLDC
EEEEEEEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
DVLQADGGTRTAAITGAYVALADAVEHLRSTGALTGEPLTGSVAAVSVGIIDGVPRLDLP
EEEECCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHEEEEHHCCCCCCCCC
YVEDVRAETDMNVVMTGSGKFVEVQGTAEGAAFDRAELDALLALAEKGCADLTRLQQEAL
HHHHHHCCCCCEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHH
SR
CC
>Mature Secondary Structure 
SAAPREDGRADDELRPITITRHWLDHAAGSVLVEFGRTRVLCAASAAEGVPRWRKGSGL
CCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCEEEEECHHHHCCCCCCCCCCC
GWVTAEYAMLPASTNTRSDRESVKGRIGGRTHEISRLIGRSLRAIIDYKALGENTIQLDC
EEEEEEEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
DVLQADGGTRTAAITGAYVALADAVEHLRSTGALTGEPLTGSVAAVSVGIIDGVPRLDLP
EEEECCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHEEEEHHCCCCCCCCC
YVEDVRAETDMNVVMTGSGKFVEVQGTAEGAAFDRAELDALLALAEKGCADLTRLQQEAL
HHHHHHCCCCCEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHH
SR
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA