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The map label for this gene is prfB [H]

Identifier: 119715500

GI number: 119715500

Start: 1338433

End: 1339560

Strand: Direct

Name: prfB [H]

Synonym: Noca_1264

Alternate gene names: 119715500

Gene position: 1338433-1339560 (Clockwise)

Preceding gene: 119715499

Following gene: 119715501

Centisome position: 26.84

GC content: 68.79

Gene sequence:

>1128_bases
TTGGTCCTTGTGGCAGGACCGGACTACGACACCGAGATCAAGCAGCTCCAGGCGACGATGCACACCATCGAGCAGGTGCT
CGACCTGGAGACCATGCGCGCCGAGATCGCCGACCTCGGCGAGCAGGTCGCCGCGCCCGACCTCTGGGACGACCAGGACA
ACGCCACCCGGGTGACCGGGCGCCTCTCCGCCCTCCAAGGTGAGCTGGACCGCTTCACCGCGCTCCAGGGCAGCGTCGAC
GACCTGGCGATCATGGTGGAGATGGCCCAGGAGGAGGGCGACGCCGACGCACTCGCCGAGGCCGAGGCCGAGCTCGGCCG
CCTGAAGCGGTCGGTGGAGGCCCTCGAGGTCCGCACCCTGCTCTCCGGCGAGTACGACGCCCGCGAGGCGATCGTCACGA
TCCGCTCCGGCGCCGGCGGCGTCGACGCGGCCGACTTCGCCGAGATGCTGATGCGGATGTACACGCGCTGGGCCGAGCAG
CACAAGTACCCCGTCGAGGTCTTCGACGTCTCCTACGCCGAGGAGGCTGGCATCAAGTCGGCGGAGTTCGCGATCCACGC
GCCGTATGCCTACGGCACCCTCTCGGTCGAGGCCGGCACGCACCGGCTGGTCCGGATCAGCCCGTTCGACAACCAGGGCC
GTCGCCAGACCTCGTTCGCGGCGGTCGAGGTGGTGCCGGTCCTGGAGCAGACCGACGAGATCGAGATCGACGAGAACGAC
ATCCGCACCGACGTGTTCCGCTCCGGCGGCCCGGGCGGCCAGTCGGTCAACACCACCGACTCCGCCGTCCGCCTGACCCA
CATCCCGACCGGCATCGTCGTCAGCTGCCAGAACGAGAAGTCGCAGCTTCAGAACAAGGCCTCCGCGATGGTGGTCCTCA
AGGCCAAGCTGCTGGCGAAGAAGAAGGCCGAGGAGGCCGCGCTCAAGAAGGACCTGAAGGGTGATGTCGCGGCCGCGTGG
GGCGACCAGATGCGCAACTACGTGCTGAACCCCTACCAGATGGTCAAGGACCTGCGCACCGGCTACGAGGTCGGCAACCC
GCAGTCGGTCTTCGACGGCGACGTCGACGACTTCATCGAGGCCGGCATCCGCTGGCGCCGCGGCGCGGAGAAGGCCGCCG
CCAACTGA

Upstream 100 bases:

>100_bases
TCGGCCGGCAGCGGGAAGCGGGTCTGGGCCGAGCTTCCGCTGTCGATCGCGGTCGCCGAGTAGGCGGCCGAGTAGGCCGG
GGCCGCGGCGCACCGTAACC

Downstream 100 bases:

>100_bases
CGACTCGGCCAACTGGCCAGAACGTGTTCTAAGGTCGGTCCATGCGGCGGGACCACTGGAAGAGGCGCAACGACTCGCTC
GACCCGGAGACCGACTTCGT

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MVLVAGPDYDTEIKQLQATMHTIEQVLDLETMRAEIADLGEQVAAPDLWDDQDNATRVTGRLSALQGELDRFTALQGSVD
DLAIMVEMAQEEGDADALAEAEAELGRLKRSVEALEVRTLLSGEYDAREAIVTIRSGAGGVDAADFAEMLMRMYTRWAEQ
HKYPVEVFDVSYAEEAGIKSAEFAIHAPYAYGTLSVEAGTHRLVRISPFDNQGRRQTSFAAVEVVPVLEQTDEIEIDEND
IRTDVFRSGGPGGQSVNTTDSAVRLTHIPTGIVVSCQNEKSQLQNKASAMVVLKAKLLAKKKAEEAALKKDLKGDVAAAW
GDQMRNYVLNPYQMVKDLRTGYEVGNPQSVFDGDVDDFIEAGIRWRRGAEKAAAN

Sequences:

>Translated_375_residues
MVLVAGPDYDTEIKQLQATMHTIEQVLDLETMRAEIADLGEQVAAPDLWDDQDNATRVTGRLSALQGELDRFTALQGSVD
DLAIMVEMAQEEGDADALAEAEAELGRLKRSVEALEVRTLLSGEYDAREAIVTIRSGAGGVDAADFAEMLMRMYTRWAEQ
HKYPVEVFDVSYAEEAGIKSAEFAIHAPYAYGTLSVEAGTHRLVRISPFDNQGRRQTSFAAVEVVPVLEQTDEIEIDEND
IRTDVFRSGGPGGQSVNTTDSAVRLTHIPTGIVVSCQNEKSQLQNKASAMVVLKAKLLAKKKAEEAALKKDLKGDVAAAW
GDQMRNYVLNPYQMVKDLRTGYEVGNPQSVFDGDVDDFIEAGIRWRRGAEKAAAN
>Mature_375_residues
MVLVAGPDYDTEIKQLQATMHTIEQVLDLETMRAEIADLGEQVAAPDLWDDQDNATRVTGRLSALQGELDRFTALQGSVD
DLAIMVEMAQEEGDADALAEAEAELGRLKRSVEALEVRTLLSGEYDAREAIVTIRSGAGGVDAADFAEMLMRMYTRWAEQ
HKYPVEVFDVSYAEEAGIKSAEFAIHAPYAYGTLSVEAGTHRLVRISPFDNQGRRQTSFAAVEVVPVLEQTDEIEIDEND
IRTDVFRSGGPGGQSVNTTDSAVRLTHIPTGIVVSCQNEKSQLQNKASAMVVLKAKLLAKKKAEEAALKKDLKGDVAAAW
GDQMRNYVLNPYQMVKDLRTGYEVGNPQSVFDGDVDDFIEAGIRWRRGAEKAAAN

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=281, Percent_Identity=32.7402135231317, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI34577120, Length=247, Percent_Identity=34.0080971659919, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI166795305, Length=189, Percent_Identity=33.8624338624339, Blast_Score=99, Evalue=7e-21,
Organism=Escherichia coli, GI2367172, Length=359, Percent_Identity=42.0612813370474, Blast_Score=285, Evalue=4e-78,
Organism=Escherichia coli, GI1787462, Length=252, Percent_Identity=40.4761904761905, Blast_Score=176, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17542784, Length=263, Percent_Identity=28.8973384030418, Blast_Score=93, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6321295, Length=351, Percent_Identity=32.7635327635328, Blast_Score=150, Evalue=4e-37,
Organism=Drosophila melanogaster, GI19921226, Length=243, Percent_Identity=33.3333333333333, Blast_Score=127, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 41101; Mature: 41101

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLVAGPDYDTEIKQLQATMHTIEQVLDLETMRAEIADLGEQVAAPDLWDDQDNATRVTG
CEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
RLSALQGELDRFTALQGSVDDLAIMVEMAQEEGDADALAEAEAELGRLKRSVEALEVRTL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LSGEYDAREAIVTIRSGAGGVDAADFAEMLMRMYTRWAEQHKYPVEVFDVSYAEEAGIKS
HCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHCCCCC
AEFAIHAPYAYGTLSVEAGTHRLVRISPFDNQGRRQTSFAAVEVVPVLEQTDEIEIDEND
CCEEEECCEEEEEEEEECCCCEEEEECCCCCCCCCHHHHHHEEEEHHHCCCCCCEECCCH
IRTDVFRSGGPGGQSVNTTDSAVRLTHIPTGIVVSCQNEKSQLQNKASAMVVLKAKLLAK
HHHHHHHCCCCCCCCCCCCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHH
KKAEEAALKKDLKGDVAAAWGDQMRNYVLNPYQMVKDLRTGYEVGNPQSVFDGDVDDFIE
HHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHCCCCHHHHHH
AGIRWRRGAEKAAAN
HCHHHHCCCHHHCCC
>Mature Secondary Structure
MVLVAGPDYDTEIKQLQATMHTIEQVLDLETMRAEIADLGEQVAAPDLWDDQDNATRVTG
CEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
RLSALQGELDRFTALQGSVDDLAIMVEMAQEEGDADALAEAEAELGRLKRSVEALEVRTL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LSGEYDAREAIVTIRSGAGGVDAADFAEMLMRMYTRWAEQHKYPVEVFDVSYAEEAGIKS
HCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHCCCCC
AEFAIHAPYAYGTLSVEAGTHRLVRISPFDNQGRRQTSFAAVEVVPVLEQTDEIEIDEND
CCEEEECCEEEEEEEEECCCCEEEEECCCCCCCCCHHHHHHEEEEHHHCCCCCCEECCCH
IRTDVFRSGGPGGQSVNTTDSAVRLTHIPTGIVVSCQNEKSQLQNKASAMVVLKAKLLAK
HHHHHHHCCCCCCCCCCCCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHH
KKAEEAALKKDLKGDVAAAWGDQMRNYVLNPYQMVKDLRTGYEVGNPQSVFDGDVDDFIE
HHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHCCCCHHHHHH
AGIRWRRGAEKAAAN
HCHHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA