BacMap

Definition	Nocardioides sp. JS614 chromosome, complete genome.
Accession	NC_008699
Length	4,985,871

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The map label for this gene is gno [H]

Identifier: 119715413

GI number: 119715413

Start: 1250472

End: 1251278

Strand: Reverse

Name: gno [H]

Synonym: Noca_1177

Alternate gene names: 119715413

Gene position: 1251278-1250472 (Counterclockwise)

Preceding gene: 119715414

Following gene: 119715412

Centisome position: 25.1

GC content: 71.25

Gene sequence:

>807_bases
ATGGCGACGGCCCAGGTCAGCACGACCGAACTGCAGCGCTTCAGGCTCGACGATCGCGTGGCCATCGTCGCGGGTGGCTC
GGGCGGTGTCGGCGTCCGGACCTGCGCTGCACTCGCCGGTGTCGGCGCCAAGGTGGCCGTCATCGGCCGGTCCGAAGCGG
GACTCGCCGCGGCCCGCAGTGCCGTGGAGGACGCCGGGGGCGAGGCTCTGGTCATCACCGGAGACATGACTCAGAAGGAG
GCCGCCGATGACGCCATCGCGCAGACCCTCGGTGCCTTCGGCCGCGTCGACGTCCTGGTCAACGGGATCGGCGGCGGCGC
CGGTACCGCCCTGCACGCTGCCGAGGACTACCCGCAAGCGGACTGGAACCGGATCCTCGACCTGAACCTGACGACGGCCC
TGCTGGTCTCCCAGGCCGCGGCCCGGGCCATGGTCGCCGGCGGCCGGGGCGGCGCCATCCTCAACATCAGCTCCGTGCGC
GGCCAGCTCGGCATCGACGCGGGCTTCTCAGCCTATGTCGCGGCCAAGGGCGCGCTGGACGCCTTGACCCGACAGCACGC
CACGGAATGGGCCAAGCACGACATCCGGGTGAACACCATCTCGCCGACGTTCGTGCGCACCGAGCAGGCGGCGGAGCTGC
TCGCCGCCCCCGGGTTCTACGACAACCTGGTCAGCCGGATCCCGCTGCACCGGATCGCGGAGACCGACGACCTGGTCGGC
GCCGTCCTCTTCTTCTGCACCGATGCCTCGTCCTTCGTGACCGGACAGCTGCTCACGCTCGACGGCGGCCTGACGGCCAC
CCAGTGA

Upstream 100 bases:

>100_bases
ACATCCAGACGCTCAACGCCGCGTCGCTGTGGGACAAGTTCACCACCAAGCGCTCCCCTGCCCGCTGAGCGCGCACACCC
ACTTCGACCGGAGGAACTTC

Downstream 100 bases:

>100_bases
GTGATCCGTCCGTGACCACCACGGCCACGTGTGCCGTCGTCCGCGAGACGGGCGGCCCGTTCCGGCTCGAGCAGGTCGAG
CTCTCGGGGCCTCGGCCGGA

Product: short-chain dehydrogenase/reductase SDR

Products: NA

Alternate protein names: 5-keto-D-gluconate 5-reductase [H]

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MATAQVSTTELQRFRLDDRVAIVAGGSGGVGVRTCAALAGVGAKVAVIGRSEAGLAAARSAVEDAGGEALVITGDMTQKE
AADDAIAQTLGAFGRVDVLVNGIGGGAGTALHAAEDYPQADWNRILDLNLTTALLVSQAAARAMVAGGRGGAILNISSVR
GQLGIDAGFSAYVAAKGALDALTRQHATEWAKHDIRVNTISPTFVRTEQAAELLAAPGFYDNLVSRIPLHRIAETDDLVG
AVLFFCTDASSFVTGQLLTLDGGLTATQ

Sequences:

>Translated_268_residues
MATAQVSTTELQRFRLDDRVAIVAGGSGGVGVRTCAALAGVGAKVAVIGRSEAGLAAARSAVEDAGGEALVITGDMTQKE
AADDAIAQTLGAFGRVDVLVNGIGGGAGTALHAAEDYPQADWNRILDLNLTTALLVSQAAARAMVAGGRGGAILNISSVR
GQLGIDAGFSAYVAAKGALDALTRQHATEWAKHDIRVNTISPTFVRTEQAAELLAAPGFYDNLVSRIPLHRIAETDDLVG
AVLFFCTDASSFVTGQLLTLDGGLTATQ
>Mature_267_residues
ATAQVSTTELQRFRLDDRVAIVAGGSGGVGVRTCAALAGVGAKVAVIGRSEAGLAAARSAVEDAGGEALVITGDMTQKEA
ADDAIAQTLGAFGRVDVLVNGIGGGAGTALHAAEDYPQADWNRILDLNLTTALLVSQAAARAMVAGGRGGAILNISSVRG
QLGIDAGFSAYVAAKGALDALTRQHATEWAKHDIRVNTISPTFVRTEQAAELLAAPGFYDNLVSRIPLHRIAETDDLVGA
VLFFCTDASSFVTGQLLTLDGGLTATQ

Specific function: Involved in the non-phosphorylative, ketogenic oxidation of glucose and oxidizes gluconate to 5-ketogluconate. Dependent on NADP, almost inactive with NAD [H]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI7705925, Length=248, Percent_Identity=35.4838709677419, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI40254992, Length=247, Percent_Identity=34.0080971659919, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI15277342, Length=268, Percent_Identity=30.5970149253731, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI19923817, Length=258, Percent_Identity=29.0697674418605, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI32483357, Length=257, Percent_Identity=28.0155642023346, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI10190704, Length=254, Percent_Identity=30.7086614173228, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI5031737, Length=253, Percent_Identity=30.8300395256917, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI4503301, Length=229, Percent_Identity=24.8908296943231, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI126723750, Length=260, Percent_Identity=27.6923076923077, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI59889578, Length=236, Percent_Identity=29.2372881355932, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI4758504, Length=260, Percent_Identity=30.3846153846154, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI66933014, Length=256, Percent_Identity=28.125, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI20149619, Length=210, Percent_Identity=28.0952380952381, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI33667109, Length=199, Percent_Identity=32.1608040201005, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI31542939, Length=201, Percent_Identity=28.3582089552239, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1790717, Length=253, Percent_Identity=33.201581027668, Blast_Score=135, Evalue=2e-33,
Organism=Escherichia coli, GI87082160, Length=263, Percent_Identity=31.9391634980989, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1789208, Length=261, Percent_Identity=31.8007662835249, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI2367175, Length=251, Percent_Identity=33.0677290836653, Blast_Score=110, Evalue=8e-26,
Organism=Escherichia coli, GI1787905, Length=258, Percent_Identity=32.5581395348837, Blast_Score=110, Evalue=9e-26,
Organism=Escherichia coli, GI1788459, Length=252, Percent_Identity=31.3492063492063, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1787335, Length=251, Percent_Identity=30.6772908366534, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI87082100, Length=191, Percent_Identity=31.9371727748691, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1789057, Length=262, Percent_Identity=29.0076335877863, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI2367365, Length=249, Percent_Identity=30.1204819277108, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1789378, Length=255, Percent_Identity=23.5294117647059, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787526, Length=201, Percent_Identity=28.8557213930348, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1786812, Length=255, Percent_Identity=27.0588235294118, Blast_Score=61, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI115534694, Length=253, Percent_Identity=31.6205533596838, Blast_Score=123, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI17555706, Length=249, Percent_Identity=32.9317269076305, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17560332, Length=262, Percent_Identity=33.206106870229, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17551412, Length=269, Percent_Identity=33.0855018587361, Blast_Score=114, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17562910, Length=267, Percent_Identity=29.2134831460674, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17560150, Length=264, Percent_Identity=28.7878787878788, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17538480, Length=262, Percent_Identity=32.824427480916, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17559104, Length=267, Percent_Identity=31.0861423220974, Blast_Score=103, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17562904, Length=266, Percent_Identity=30.4511278195489, Blast_Score=103, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17562906, Length=268, Percent_Identity=28.7313432835821, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17538486, Length=262, Percent_Identity=32.0610687022901, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17560676, Length=253, Percent_Identity=27.6679841897233, Blast_Score=100, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17562908, Length=267, Percent_Identity=28.8389513108614, Blast_Score=100, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI193204405, Length=265, Percent_Identity=28.6792452830189, Blast_Score=100, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI25147288, Length=251, Percent_Identity=29.4820717131474, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17531453, Length=262, Percent_Identity=29.0076335877863, Blast_Score=97, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17560220, Length=261, Percent_Identity=28.3524904214559, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17544670, Length=273, Percent_Identity=27.1062271062271, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17562990, Length=266, Percent_Identity=27.8195488721804, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17565030, Length=272, Percent_Identity=30.8823529411765, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71994600, Length=255, Percent_Identity=27.843137254902, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI71994604, Length=253, Percent_Identity=27.6679841897233, Blast_Score=87, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17561402, Length=260, Percent_Identity=26.9230769230769, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI72000259, Length=265, Percent_Identity=26.0377358490566, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17561272, Length=186, Percent_Identity=29.0322580645161, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17536025, Length=255, Percent_Identity=26.2745098039216, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17563726, Length=264, Percent_Identity=24.2424242424242, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17536651, Length=252, Percent_Identity=25.7936507936508, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17568967, Length=199, Percent_Identity=31.1557788944724, Blast_Score=67, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17538182, Length=254, Percent_Identity=27.5590551181102, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6324126, Length=251, Percent_Identity=26.2948207171315, Blast_Score=92, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6322861, Length=194, Percent_Identity=31.9587628865979, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI23397609, Length=254, Percent_Identity=33.0708661417323, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24639444, Length=249, Percent_Identity=29.3172690763052, Blast_Score=100, Evalue=9e-22,
Organism=Drosophila melanogaster, GI21357041, Length=253, Percent_Identity=29.6442687747036, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24643142, Length=250, Percent_Identity=28.4, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21355319, Length=252, Percent_Identity=24.2063492063492, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI28571526, Length=255, Percent_Identity=29.8039215686275, Blast_Score=91, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24644339, Length=252, Percent_Identity=29.3650793650794, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24644337, Length=231, Percent_Identity=28.1385281385281, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI18859837, Length=200, Percent_Identity=33, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.1.1.69 [H]

Molecular weight: Translated: 27346; Mature: 27215

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATAQVSTTELQRFRLDDRVAIVAGGSGGVGVRTCAALAGVGAKVAVIGRSEAGLAAARS
CCCCCCCHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHH
AVEDAGGEALVITGDMTQKEAADDAIAQTLGAFGRVDVLVNGIGGGAGTALHAAEDYPQA
HHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEECCCCCCCCC
DWNRILDLNLTTALLVSQAAARAMVAGGRGGAILNISSVRGQLGIDAGFSAYVAAKGALD
CHHEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEEEHHCCCCCCCCCCCHHHHHHHHHHH
ALTRQHATEWAKHDIRVNTISPTFVRTEQAAELLAAPGFYDNLVSRIPLHRIAETDDLVG
HHHHHHHHHHHHCCEEEEECCCHHEEHHHHHHHHHCCCHHHHHHHHCCHHHHCCCHHHHH
AVLFFCTDASSFVTGQLLTLDGGLTATQ
HHHHHHCCCCHHHEEEEEEECCCCCCCC
>Mature Secondary Structure 
ATAQVSTTELQRFRLDDRVAIVAGGSGGVGVRTCAALAGVGAKVAVIGRSEAGLAAARS
CCCCCCHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHH
AVEDAGGEALVITGDMTQKEAADDAIAQTLGAFGRVDVLVNGIGGGAGTALHAAEDYPQA
HHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEECCCCCCCCC
DWNRILDLNLTTALLVSQAAARAMVAGGRGGAILNISSVRGQLGIDAGFSAYVAAKGALD
CHHEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEEEHHCCCCCCCCCCCHHHHHHHHHHH
ALTRQHATEWAKHDIRVNTISPTFVRTEQAAELLAAPGFYDNLVSRIPLHRIAETDDLVG
HHHHHHHHHHHHCCEEEEECCCHHEEHHHHHHHHHCCCHHHHHHHHCCHHHHCCCHHHHH
AVLFFCTDASSFVTGQLLTLDGGLTATQ
HHHHHHCCCCHHHEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7751271 [H]