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The map label for this gene is rihA [H]

Identifier: 119714806

GI number: 119714806

Start: 576702

End: 577691

Strand: Reverse

Name: rihA [H]

Synonym: Noca_0542

Alternate gene names: 119714806

Gene position: 577691-576702 (Counterclockwise)

Preceding gene: 119714807

Following gene: 119714801

Centisome position: 11.59

GC content: 69.29

Gene sequence:

>990_bases
GTGCCCGTCCCGTTCATCCTCGACACCGACACCGCGCAAGACGACTGCGTGGCCATCATCGCCGGGATCCTCGACCCCGA
GGCCGACCTGCGGGCGCTCACGATGGTCGCCGGCAACGTCAGCTTCGACCAGCAGGTCCGCAACGCCCAGCTCACCTTGA
ACGCGCTCGGCCGGCTCGGCGAGGTGCCCATTCACCTCGGCTGCCGGCAGCCGATGGTCCTGCCGTGGGTATCGGCCGAG
AACGTGCACAGCGACGGCTCCGGCGGGCTGGACATGGACTTCGCGGGCACCACGACGGAGGACGAGCACGCGGTCGACGC
GTTGATCCGGATGACCGCCGAGGCGCCCGGCGAGATCTCGGTGGTGGCGATCGGTCCGCTCACGAACATCGCGATGGCCG
CGGTCAAGGACCCCGCGTTCGTCCGCAACGTCCGGCACCTGGTGATCATGGGCGGCTCCAACAACGGCCGCGGCAACATC
ACCCCGGCGGCCGAGTTCAACCTCTACGTCGACCCCCACGCCGCCAAGGTGGTCTTCGAGGCCGGCTTCGACATCACCGT
CGTGCCGTGGGCACCGCTGACACTCAACGACGCCGTGTTCAGCCGCGAGCAGCTCGCGGGCATCGAGTCGCTCGGTACGA
CACTGTCCCAGTTCTTCCTGCAGGTGTGCGGGCCCACGCTGGCCTTCGACGAAAGCGTGGGCATCCCGGGCACGACCCAC
CCCGACTCGCTCTCGGTGGCCGTGCTGCTGCATCCCGACCTGGTCCTCGATGCCGCGGAGTACCACGTCGCGGTCGAGGC
CGACTCGGAGCTGACCCGCGGGTACTCGTCGATGTCGTGGGGCGTGCACGGCCTGGCTCCCAACGCCCGGGTGATCGAGC
ACATCGACGCCGTGGCGTTCCATCGCTACCTGACTGGACTCCTGGCCACTCCCACCGAGCCCACCGTTCCACTCCGGTTC
GGCGCCCCGTTCACGGCTCGCCAGGGCTGA

Upstream 100 bases:

>100_bases
CGACGGCTCGCCGATCGAGTACTCCGACGTCTGGATCCGCTCCGACCGGCTCCGCGTCACCTCCCTGCTGACCCGCCACT
GACCTCCACAGGAGACCCTC

Downstream 100 bases:

>100_bases
CCCGACGTTCCACCTAGGCGGGGGTGGGCAACATGGCGGGGATGACGGCGGCACCCGAGGCCGGGGTGACCCACACCGGC
GAACCGGCCTCGAGTCCGAG

Product: inosine/uridine-preferring nucleoside hydrolase

Products: cytosine; ribose; uracil [C]

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MPVPFILDTDTAQDDCVAIIAGILDPEADLRALTMVAGNVSFDQQVRNAQLTLNALGRLGEVPIHLGCRQPMVLPWVSAE
NVHSDGSGGLDMDFAGTTTEDEHAVDALIRMTAEAPGEISVVAIGPLTNIAMAAVKDPAFVRNVRHLVIMGGSNNGRGNI
TPAAEFNLYVDPHAAKVVFEAGFDITVVPWAPLTLNDAVFSREQLAGIESLGTTLSQFFLQVCGPTLAFDESVGIPGTTH
PDSLSVAVLLHPDLVLDAAEYHVAVEADSELTRGYSSMSWGVHGLAPNARVIEHIDAVAFHRYLTGLLATPTEPTVPLRF
GAPFTARQG

Sequences:

>Translated_329_residues
MPVPFILDTDTAQDDCVAIIAGILDPEADLRALTMVAGNVSFDQQVRNAQLTLNALGRLGEVPIHLGCRQPMVLPWVSAE
NVHSDGSGGLDMDFAGTTTEDEHAVDALIRMTAEAPGEISVVAIGPLTNIAMAAVKDPAFVRNVRHLVIMGGSNNGRGNI
TPAAEFNLYVDPHAAKVVFEAGFDITVVPWAPLTLNDAVFSREQLAGIESLGTTLSQFFLQVCGPTLAFDESVGIPGTTH
PDSLSVAVLLHPDLVLDAAEYHVAVEADSELTRGYSSMSWGVHGLAPNARVIEHIDAVAFHRYLTGLLATPTEPTVPLRF
GAPFTARQG
>Mature_328_residues
PVPFILDTDTAQDDCVAIIAGILDPEADLRALTMVAGNVSFDQQVRNAQLTLNALGRLGEVPIHLGCRQPMVLPWVSAEN
VHSDGSGGLDMDFAGTTTEDEHAVDALIRMTAEAPGEISVVAIGPLTNIAMAAVKDPAFVRNVRHLVIMGGSNNGRGNIT
PAAEFNLYVDPHAAKVVFEAGFDITVVPWAPLTLNDAVFSREQLAGIESLGTTLSQFFLQVCGPTLAFDESVGIPGTTHP
DSLSVAVLLHPDLVLDAAEYHVAVEADSELTRGYSSMSWGVHGLAPNARVIEHIDAVAFHRYLTGLLATPTEPTVPLRFG
APFTARQG

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788486, Length=305, Percent_Identity=33.7704918032787, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1786871, Length=309, Percent_Identity=32.0388349514563, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1786213, Length=319, Percent_Identity=35.1097178683386, Blast_Score=119, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI32565474, Length=294, Percent_Identity=31.2925170068027, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17565698, Length=302, Percent_Identity=28.476821192053, Blast_Score=90, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6320608, Length=238, Percent_Identity=28.9915966386555, Blast_Score=97, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24641839, Length=332, Percent_Identity=28.6144578313253, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24641837, Length=335, Percent_Identity=30.7462686567164, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24583915, Length=331, Percent_Identity=28.3987915407855, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 34905; Mature: 34774

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVPFILDTDTAQDDCVAIIAGILDPEADLRALTMVAGNVSFDQQVRNAQLTLNALGRLG
CCCCEEEECCCCCHHHHHHHHHHCCCCCHHEEEEEEECCCCHHHHHHHHEEEHHHHHCCC
EVPIHLGCRQPMVLPWVSAENVHSDGSGGLDMDFAGTTTEDEHAVDALIRMTAEAPGEIS
CCCEEECCCCCEEEEECCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCCEE
VVAIGPLTNIAMAAVKDPAFVRNVRHLVIMGGSNNGRGNITPAAEFNLYVDPHAAKVVFE
EEEECCHHHHHHHHHCCCHHHHCCEEEEEEECCCCCCCCCCCCEEEEEEECCCCEEEEEE
AGFDITVVPWAPLTLNDAVFSREQLAGIESLGTTLSQFFLQVCGPTLAFDESVGIPGTTH
CCCCEEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCC
PDSLSVAVLLHPDLVLDAAEYHVAVEADSELTRGYSSMSWGVHGLAPNARVIEHIDAVAF
CCCEEEEEEECCCCEEEHHHEEEEEECCCHHHCCCCHHCCCCCCCCCCHHHHHHHHHHHH
HRYLTGLLATPTEPTVPLRFGAPFTARQG
HHHHHHHHCCCCCCCEEEEECCCCCCCCC
>Mature Secondary Structure 
PVPFILDTDTAQDDCVAIIAGILDPEADLRALTMVAGNVSFDQQVRNAQLTLNALGRLG
CCCEEEECCCCCHHHHHHHHHHCCCCCHHEEEEEEECCCCHHHHHHHHEEEHHHHHCCC
EVPIHLGCRQPMVLPWVSAENVHSDGSGGLDMDFAGTTTEDEHAVDALIRMTAEAPGEIS
CCCEEECCCCCEEEEECCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCCEE
VVAIGPLTNIAMAAVKDPAFVRNVRHLVIMGGSNNGRGNITPAAEFNLYVDPHAAKVVFE
EEEECCHHHHHHHHHCCCHHHHCCEEEEEEECCCCCCCCCCCCEEEEEEECCCCEEEEEE
AGFDITVVPWAPLTLNDAVFSREQLAGIESLGTTLSQFFLQVCGPTLAFDESVGIPGTTH
CCCCEEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCC
PDSLSVAVLLHPDLVLDAAEYHVAVEADSELTRGYSSMSWGVHGLAPNARVIEHIDAVAF
CCCEEEEEEECCCCEEEHHHEEEEEECCCHHHCCCCHHCCCCCCCCCCHHHHHHHHHHHH
HRYLTGLLATPTEPTVPLRFGAPFTARQG
HHHHHHHHCCCCCCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: cytidine; H2O; uridine [C]

Specific reaction: cytidine + H2O = cytosine + ribose uridine + H2O = uracil + ribose [C]

General reaction: Pyrimidine-specific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA