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The map label for this gene is menF [H]

Identifier: 119714780

GI number: 119714780

Start: 547044

End: 548330

Strand: Reverse

Name: menF [H]

Synonym: Noca_0516

Alternate gene names: 119714780

Gene position: 548330-547044 (Counterclockwise)

Preceding gene: 119714782

Following gene: 119714778

Centisome position: 11.0

GC content: 74.36

Gene sequence:

>1287_bases
GTGAGTACCCTCGGCAGGGTGACGAGCAGCCCCACACCCGGCGATCCGCCGCCGGCCCCGGCGGACGCGCTCGTCGTCCG
CACGGTCCGCCTCGATCTCGACCAGGCGGCGAGCCTGCTGGACCTGGTCCCGGCCGACCGGCCGGTCACCTGGTTGCGGC
GGGGCGACGGCCTGGTCGGTTGGGGCGTCGCCGCCCAGGTGCGCACCAGCGGGGCCACCCGGTTCGCCGACGCCGTGAAG
TGGTGGAGCGAGACCATCGCTCGGGCGGACGTGCACGACGACGTCAACGAGCCTGGCACCGGCCTCGTGTCGTTCGGGTC
GTTCGCGTTCGCCGACGACCCGGGCGACTCGATCCTGGTCCTCCCCCGCGTGGTCGTCGGACGCCGCGGCGACGTCACGT
GGCTCACCACGGTCGGCGAGGTCGGACCCCCGCTCGGGCCGGTGGAGCGTCCAGCGGCCCCGGCCGCGGTCAGCTTCGCC
GACGGCGCGCTGAACGGCGAGCAGTGGATGTCCGTGGTCGCCGACGCCGTGGCACGGATCAACGCCGGCGACCTGGAGAA
GGTCGTGCTCGCTCGGGACCTGGTCGCCACGGCGAGCGACCCGGTCGACGTACGCTGGCCGTTGGGCCGGCTCGGCGAGA
CCTATCCGATGTGCTGGACGTTCCACGTCGACGGCATGTTCGGGGCGACCCCGGAGATGCTGGTGCGCCGCGAGCGCGGC
CTGGTGACCTCGCGGGTGCTGGCCGGCACCATCCGCCGCACCGGCGACCACGAGCGCGACCTGGCCCTGGCCGCGACCCT
GGCCCATTCCTCCAAGGACCTCGAGGAGCACGAGTACGCCGTCCGCTCGGTCGCCGACGCGCTCGAGCCGCACTGCTCGT
CGATGAACGTGCCCGAGGCTCCGTTCGTGCTGCACCTGCCGAACGTCATGCACCTGGCGACCGACGTGGCCGGCGTGGTC
CATGACGCGGCCACCGTGTCCTCGCTCCAGCTCGCCGAGGCGCTGCACCCGTCGGCCGCCGTCGGCGGCACGCCGACCCC
GGTCGCGACCGCGTTGATCGCGGACATCGAGGGCATGGACCGCGACCGCTACGCCGGGCCGGTCGGGTGGATGGACGCCT
CCGGCGACGGCGAGTGGGGCATCGCCCTGCGCTCCGCCCGTCTCGAGGGCCGCACCGTCCGGCTGTTCGCCGGCTGCGGC
ATCGTCGCCGACTCCGACCCAGCCGCGGAGCTCGCCGAGGCCCAGGCCAAGTTCGTCCCGGTCCGCGACGCGCTCACCGC
GTCCTGA

Upstream 100 bases:

>100_bases
CCCGCCCAGGTGGAGGGACGGCCGGACGTCGGGGCGGAGGTGCGGCTGGTCACGTCCGGCGACGCTACCGGGCCCGTGGG
TGCGGAGGTTGGCGGGGTCC

Downstream 100 bases:

>100_bases
CGGACCGGCGGGCGGTTTCCCCGGTTCACCGGGGAAGTTGGAACTTCTGGCCCGAAGTTTCGGGTCAACAGTTCCGGTTT
CCCCGGTTCACCGGGGAAAC

Product: isochorismate synthases

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MSTLGRVTSSPTPGDPPPAPADALVVRTVRLDLDQAASLLDLVPADRPVTWLRRGDGLVGWGVAAQVRTSGATRFADAVK
WWSETIARADVHDDVNEPGTGLVSFGSFAFADDPGDSILVLPRVVVGRRGDVTWLTTVGEVGPPLGPVERPAAPAAVSFA
DGALNGEQWMSVVADAVARINAGDLEKVVLARDLVATASDPVDVRWPLGRLGETYPMCWTFHVDGMFGATPEMLVRRERG
LVTSRVLAGTIRRTGDHERDLALAATLAHSSKDLEEHEYAVRSVADALEPHCSSMNVPEAPFVLHLPNVMHLATDVAGVV
HDAATVSSLQLAEALHPSAAVGGTPTPVATALIADIEGMDRDRYAGPVGWMDASGDGEWGIALRSARLEGRTVRLFAGCG
IVADSDPAAELAEAQAKFVPVRDALTAS

Sequences:

>Translated_428_residues
MSTLGRVTSSPTPGDPPPAPADALVVRTVRLDLDQAASLLDLVPADRPVTWLRRGDGLVGWGVAAQVRTSGATRFADAVK
WWSETIARADVHDDVNEPGTGLVSFGSFAFADDPGDSILVLPRVVVGRRGDVTWLTTVGEVGPPLGPVERPAAPAAVSFA
DGALNGEQWMSVVADAVARINAGDLEKVVLARDLVATASDPVDVRWPLGRLGETYPMCWTFHVDGMFGATPEMLVRRERG
LVTSRVLAGTIRRTGDHERDLALAATLAHSSKDLEEHEYAVRSVADALEPHCSSMNVPEAPFVLHLPNVMHLATDVAGVV
HDAATVSSLQLAEALHPSAAVGGTPTPVATALIADIEGMDRDRYAGPVGWMDASGDGEWGIALRSARLEGRTVRLFAGCG
IVADSDPAAELAEAQAKFVPVRDALTAS
>Mature_427_residues
STLGRVTSSPTPGDPPPAPADALVVRTVRLDLDQAASLLDLVPADRPVTWLRRGDGLVGWGVAAQVRTSGATRFADAVKW
WSETIARADVHDDVNEPGTGLVSFGSFAFADDPGDSILVLPRVVVGRRGDVTWLTTVGEVGPPLGPVERPAAPAAVSFAD
GALNGEQWMSVVADAVARINAGDLEKVVLARDLVATASDPVDVRWPLGRLGETYPMCWTFHVDGMFGATPEMLVRRERGL
VTSRVLAGTIRRTGDHERDLALAATLAHSSKDLEEHEYAVRSVADALEPHCSSMNVPEAPFVLHLPNVMHLATDVAGVVH
DAATVSSLQLAEALHPSAAVGGTPTPVATALIADIEGMDRDRYAGPVGWMDASGDGEWGIALRSARLEGRTVRLFAGCGI
VADSDPAAELAEAQAKFVPVRDALTAS

Specific function: Siderophore biosynthesis; enterobactin biosynthesis. [C]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786809, Length=279, Percent_Identity=36.9175627240143, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI87082077, Length=262, Percent_Identity=30.9160305343511, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1787518, Length=296, Percent_Identity=29.3918918918919, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1788114, Length=258, Percent_Identity=24.8062015503876, Blast_Score=69, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6320935, Length=285, Percent_Identity=28.0701754385965, Blast_Score=84, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 45063; Mature: 44932

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLGRVTSSPTPGDPPPAPADALVVRTVRLDLDQAASLLDLVPADRPVTWLRRGDGLVG
CCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHCHHHHHHHHHHCCCCCCCHHHHCCCCEEE
WGVAAQVRTSGATRFADAVKWWSETIARADVHDDVNEPGTGLVSFGSFAFADDPGDSILV
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCEEECCCCCCCEEE
LPRVVVGRRGDVTWLTTVGEVGPPLGPVERPAAPAAVSFADGALNGEQWMSVVADAVARI
ECHHHHCCCCCEEEEEECHHCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHC
NAGDLEKVVLARDLVATASDPVDVRWPLGRLGETYPMCWTFHVDGMFGATPEMLVRRERG
CCCCHHHHHHHHHHHHHCCCCCEEECCHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHC
LVTSRVLAGTIRRTGDHERDLALAATLAHSSKDLEEHEYAVRSVADALEPHCSSMNVPEA
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
PFVLHLPNVMHLATDVAGVVHDAATVSSLQLAEALHPSAAVGGTPTPVATALIADIEGMD
CEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCC
RDRYAGPVGWMDASGDGEWGIALRSARLEGRTVRLFAGCGIVADSDPAAELAEAQAKFVP
CCCCCCCCCCCCCCCCCCEEEEEEEEECCCCEEEEEECCCEEECCCHHHHHHHHHHHEEE
VRDALTAS
HHHHHCCC
>Mature Secondary Structure 
STLGRVTSSPTPGDPPPAPADALVVRTVRLDLDQAASLLDLVPADRPVTWLRRGDGLVG
CCCCCCCCCCCCCCCCCCCCCCEEEEHHHHCHHHHHHHHHHCCCCCCCHHHHCCCCEEE
WGVAAQVRTSGATRFADAVKWWSETIARADVHDDVNEPGTGLVSFGSFAFADDPGDSILV
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCEEECCCCCCCEEE
LPRVVVGRRGDVTWLTTVGEVGPPLGPVERPAAPAAVSFADGALNGEQWMSVVADAVARI
ECHHHHCCCCCEEEEEECHHCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHC
NAGDLEKVVLARDLVATASDPVDVRWPLGRLGETYPMCWTFHVDGMFGATPEMLVRRERG
CCCCHHHHHHHHHHHHHCCCCCEEECCHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHC
LVTSRVLAGTIRRTGDHERDLALAATLAHSSKDLEEHEYAVRSVADALEPHCSSMNVPEA
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
PFVLHLPNVMHLATDVAGVVHDAATVSSLQLAEALHPSAAVGGTPTPVATALIADIEGMD
CEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCC
RDRYAGPVGWMDASGDGEWGIALRSARLEGRTVRLFAGCGIVADSDPAAELAEAQAKFVP
CCCCCCCCCCCCCCCCCCEEEEEEEEECCCCEEEEEECCCEEECCCHHHHHHHHHHHEEE
VRDALTAS
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629163; 8566759; 9387221; 9384377; 3131310 [H]