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The map label for this gene is menD

Identifier: 119714774

GI number: 119714774

Start: 540289

End: 541902

Strand: Direct

Name: menD

Synonym: Noca_0510

Alternate gene names: 119714774

Gene position: 540289-541902 (Clockwise)

Preceding gene: 119714773

Following gene: 119714775

Centisome position: 10.84

GC content: 77.26

Gene sequence:

>1614_bases
GTGACCGCCCCTGAGCCCGCCCCGGAGTCCGCCACCGCCACCGCCCGCCGGATCCTGGCGGCCCTGGTCGAGGCCGGCGT
CACCGAGATCGTGGTCGCCCCCGGGTCGCGGAACGCGCCGCTGTCGTTCGCGGCGTACGACGCCGCCGCCGCGGGCCTGG
TCCGGCTGCACACGCGCCTCGACGAGCGGACCGCCGGCTTCCTCGCGCTCGGCCTCACCAGGAGCGGCCGGCGGGCAGCC
GTGGTCTGCACGTCCGGCACCGCGGTCGCCAACCTGCACCCGGCGGTCCTCGAGGCGGCCCACGCCGGCGTCGACCTGGT
CGTCGTGTCCGCCGACCGGCCGGCCCGGCTGCGCGGCACCGGCGCGAACCAGACCACCGACCAGGTCGGCATCTTCGGCC
CCCTGGTTCCCACCCAGGACGCCAGCGGCCCGGTCGAGCTGCTGACCGCGGGCCCGGTCCATCTCAACGTCCCGCTCGAG
GAGCCGCTCGTCCCCGACGACCGCTGGCTCCCGGACGTGGTGCCGGGGGAGCCGGCCCACCGGGTGATCCCCGCGTCCCT
GACCGTGCTGGCGCGGGGGCCGCGCACGGTCGTGGTCGCCGGTGACGACGCCGGCCCGCGGGTCCGGGTCCTGGCCGAGC
AGGCCGGCTGGCCGCTGCTCGCCGAGCCGTCCAGCGGGTCGCGCACCGGGGACAACGCGATCCGCACCTACCGGCTGCTG
CTGGACGGCGACCTCGGCGCGCGCGTCGAGCGGGTCGTGGTCGGCGGTCACCCCACGCTGTCCCGCCCGGTCAGCCGGCT
GCTGGCCCGACCGGACGTGGAGGTGGTCGACCTCGGTGCCTGGGGGGTCTGGAGCGAGCGACCCTTCCCCGTGCACTCCC
GGATCCTCGGTGGCGTCGGCGTCGACGCCCCGGTCGACGCGGCCGACGGCACGGCCGACGACACGGACTGGCTGGAGGAG
TGGCGGACGGCGGACCGGGCGGTGTCCGCCCGGCTGGACGCGCTGCTCGCCGCCGAGCCCGGGCTCACGCCGCACGAGGT
CGCGGGCGCGGTCGCCCGGGCCCTGCCCGCCGGCGGGCTGCTCGTCGTCGGCGCCTCGAGCCCGATCCGCGACCTGGACC
TGATGGTGCCGCGCTACGACGTGGGCGCCCGGCGCAAGGTGGTCGCCAACCGCGGCCTCGCCGGCATCGACGGCACCATC
TCGACCGCGGTCGGCGCCGCCCTGGGCCGGCCGCGCAGCACCCGCGCCCTGACCCTGATGGGGGACGTCACGTTCCTCCA
CGACGCCGGCGCGCTGGTGATCGGGCCCGGTGAGGCGGTCCCCGACCTCACGATCGTCGTGGTCAACGACGACGGCGGTT
CGATCTTCGCGATGCTCGAGCAGGGGGCGGCGGAGTACGCCGACCAGTACGACCGGCTCTTCGGCACCCCGCACCGGGTC
GACCTCGCCAGCCTCTGCGCCGCGACCCGCACGCCCCACTGGCGGGTCGACTCCCTGGCCGAGCTCGAGCACGCCCTCGC
CTCGCCCGCGGGCGGGATCGAGGTGGTGGAGGCCGTCGTACGCCGCGACAACCGGCGCGACCTCGACGAGCGGATCCGCG
CGCTGCGTCCCTGA

Upstream 100 bases:

>100_bases
TGCAGGTCGACGAGTCGCTCGCGCCCGCCGACCCGGACAGGGTCGCCCACTGGGAGGCGCGGCTGGCCGAGGTGCGCGCG
CTGCGACAGGATCGTCCCTC

Downstream 100 bases:

>100_bases
CAGTCCGGTCCGTCCGACCGCCGGTCCGCGACAATGGCGGACGTGGACACCGCCCTCTTCCTGGTCGCGCTGGCCGTCGC
CGTGCTCGCCGGCGTGGTGG

Product: 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD

Number of amino acids: Translated: 537; Mature: 536

Protein sequence:

>537_residues
MTAPEPAPESATATARRILAALVEAGVTEIVVAPGSRNAPLSFAAYDAAAAGLVRLHTRLDERTAGFLALGLTRSGRRAA
VVCTSGTAVANLHPAVLEAAHAGVDLVVVSADRPARLRGTGANQTTDQVGIFGPLVPTQDASGPVELLTAGPVHLNVPLE
EPLVPDDRWLPDVVPGEPAHRVIPASLTVLARGPRTVVVAGDDAGPRVRVLAEQAGWPLLAEPSSGSRTGDNAIRTYRLL
LDGDLGARVERVVVGGHPTLSRPVSRLLARPDVEVVDLGAWGVWSERPFPVHSRILGGVGVDAPVDAADGTADDTDWLEE
WRTADRAVSARLDALLAAEPGLTPHEVAGAVARALPAGGLLVVGASSPIRDLDLMVPRYDVGARRKVVANRGLAGIDGTI
STAVGAALGRPRSTRALTLMGDVTFLHDAGALVIGPGEAVPDLTIVVVNDDGGSIFAMLEQGAAEYADQYDRLFGTPHRV
DLASLCAATRTPHWRVDSLAELEHALASPAGGIEVVEAVVRRDNRRDLDERIRALRP

Sequences:

>Translated_537_residues
MTAPEPAPESATATARRILAALVEAGVTEIVVAPGSRNAPLSFAAYDAAAAGLVRLHTRLDERTAGFLALGLTRSGRRAA
VVCTSGTAVANLHPAVLEAAHAGVDLVVVSADRPARLRGTGANQTTDQVGIFGPLVPTQDASGPVELLTAGPVHLNVPLE
EPLVPDDRWLPDVVPGEPAHRVIPASLTVLARGPRTVVVAGDDAGPRVRVLAEQAGWPLLAEPSSGSRTGDNAIRTYRLL
LDGDLGARVERVVVGGHPTLSRPVSRLLARPDVEVVDLGAWGVWSERPFPVHSRILGGVGVDAPVDAADGTADDTDWLEE
WRTADRAVSARLDALLAAEPGLTPHEVAGAVARALPAGGLLVVGASSPIRDLDLMVPRYDVGARRKVVANRGLAGIDGTI
STAVGAALGRPRSTRALTLMGDVTFLHDAGALVIGPGEAVPDLTIVVVNDDGGSIFAMLEQGAAEYADQYDRLFGTPHRV
DLASLCAATRTPHWRVDSLAELEHALASPAGGIEVVEAVVRRDNRRDLDERIRALRP
>Mature_536_residues
TAPEPAPESATATARRILAALVEAGVTEIVVAPGSRNAPLSFAAYDAAAAGLVRLHTRLDERTAGFLALGLTRSGRRAAV
VCTSGTAVANLHPAVLEAAHAGVDLVVVSADRPARLRGTGANQTTDQVGIFGPLVPTQDASGPVELLTAGPVHLNVPLEE
PLVPDDRWLPDVVPGEPAHRVIPASLTVLARGPRTVVVAGDDAGPRVRVLAEQAGWPLLAEPSSGSRTGDNAIRTYRLLL
DGDLGARVERVVVGGHPTLSRPVSRLLARPDVEVVDLGAWGVWSERPFPVHSRILGGVGVDAPVDAADGTADDTDWLEEW
RTADRAVSARLDALLAAEPGLTPHEVAGAVARALPAGGLLVVGASSPIRDLDLMVPRYDVGARRKVVANRGLAGIDGTIS
TAVGAALGRPRSTRALTLMGDVTFLHDAGALVIGPGEAVPDLTIVVVNDDGGSIFAMLEQGAAEYADQYDRLFGTPHRVD
LASLCAATRTPHWRVDSLAELEHALASPAGGIEVVEAVVRRDNRRDLDERIRALRP

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily

Homologues:

Organism=Escherichia coli, GI1788599, Length=563, Percent_Identity=30.7282415630551, Blast_Score=162, Evalue=4e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MEND_NOCSJ (A1SE17)

Other databases:

- EMBL:   CP000509
- RefSeq:   YP_921739.1
- ProteinModelPortal:   A1SE17
- STRING:   A1SE17
- GeneID:   4597409
- GenomeReviews:   CP000509_GR
- KEGG:   nca:Noca_0510
- eggNOG:   COG1165
- HOGENOM:   HBG634405
- OMA:   IAEPTAP
- PhylomeDB:   A1SE17
- BioCyc:   NSP35761:NOCA_0510-MONOMER
- HAMAP:   MF_01659
- InterPro:   IPR004433
- InterPro:   IPR012001
- InterPro:   IPR011766
- TIGRFAMs:   TIGR00173

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N

EC number: =2.2.1.9

Molecular weight: Translated: 56034; Mature: 55903

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00226 IF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPEPAPESATATARRILAALVEAGVTEIVVAPGSRNAPLSFAAYDAAAAGLVRLHTRL
CCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEHHHHHHHHHHHHHHHH
DERTAGFLALGLTRSGRRAAVVCTSGTAVANLHPAVLEAAHAGVDLVVVSADRPARLRGT
HHHCCCEEEEEECCCCCEEEEEEECCCEEECCCHHHHHHHHCCEEEEEEECCCCCEEECC
GANQTTDQVGIFGPLVPTQDASGPVELLTAGPVHLNVPLEEPLVPDDRWLPDVVPGEPAH
CCCCCCCCCCEEECCCCCCCCCCCEEEEEECCEEEECCCCCCCCCCCCCCCCCCCCCCCC
RVIPASLTVLARGPRTVVVAGDDAGPRVRVLAEQAGWPLLAEPSSGSRTGDNAIRTYRLL
EEECCEEEEEECCCEEEEEECCCCCCEEEEEEHHCCCCEEECCCCCCCCCCCCEEEEEEE
LDGDLGARVERVVVGGHPTLSRPVSRLLARPDVEVVDLGAWGVWSERPFPVHSRILGGVG
EECCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCHHHHHHCCCC
VDAPVDAADGTADDTDWLEEWRTADRAVSARLDALLAAEPGLTPHEVAGAVARALPAGGL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCE
LVVGASSPIRDLDLMVPRYDVGARRKVVANRGLAGIDGTISTAVGAALGRPRSTRALTLM
EEEECCCCCCHHHEECCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEE
GDVTFLHDAGALVIGPGEAVPDLTIVVVNDDGGSIFAMLEQGAAEYADQYDRLFGTPHRV
ECEEEEECCCEEEECCCCCCCCEEEEEEECCCCEEEEHHHHHHHHHHHHHHHHCCCCCCC
DLASLCAATRTPHWRVDSLAELEHALASPAGGIEVVEAVVRRDNRRDLDERIRALRP
CHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHCCC
>Mature Secondary Structure 
TAPEPAPESATATARRILAALVEAGVTEIVVAPGSRNAPLSFAAYDAAAAGLVRLHTRL
CCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEHHHHHHHHHHHHHHHH
DERTAGFLALGLTRSGRRAAVVCTSGTAVANLHPAVLEAAHAGVDLVVVSADRPARLRGT
HHHCCCEEEEEECCCCCEEEEEEECCCEEECCCHHHHHHHHCCEEEEEEECCCCCEEECC
GANQTTDQVGIFGPLVPTQDASGPVELLTAGPVHLNVPLEEPLVPDDRWLPDVVPGEPAH
CCCCCCCCCCEEECCCCCCCCCCCEEEEEECCEEEECCCCCCCCCCCCCCCCCCCCCCCC
RVIPASLTVLARGPRTVVVAGDDAGPRVRVLAEQAGWPLLAEPSSGSRTGDNAIRTYRLL
EEECCEEEEEECCCEEEEEECCCCCCEEEEEEHHCCCCEEECCCCCCCCCCCCEEEEEEE
LDGDLGARVERVVVGGHPTLSRPVSRLLARPDVEVVDLGAWGVWSERPFPVHSRILGGVG
EECCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCHHHHHHCCCC
VDAPVDAADGTADDTDWLEEWRTADRAVSARLDALLAAEPGLTPHEVAGAVARALPAGGL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCE
LVVGASSPIRDLDLMVPRYDVGARRKVVANRGLAGIDGTISTAVGAALGRPRSTRALTLM
EEEECCCCCCHHHEECCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEE
GDVTFLHDAGALVIGPGEAVPDLTIVVVNDDGGSIFAMLEQGAAEYADQYDRLFGTPHRV
ECEEEEECCCEEEECCCCCCCCEEEEEEECCCCEEEEHHHHHHHHHHHHHHHHCCCCCCC
DLASLCAATRTPHWRVDSLAELEHALASPAGGIEVVEAVVRRDNRRDLDERIRALRP
CHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA