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The map label for this gene is menE [H]

Identifier: 119714772

GI number: 119714772

Start: 538338

End: 539339

Strand: Direct

Name: menE [H]

Synonym: Noca_0508

Alternate gene names: 119714772

Gene position: 538338-539339 (Clockwise)

Preceding gene: 119714770

Following gene: 119714773

Centisome position: 10.8

GC content: 77.45

Gene sequence:

>1002_bases
GTGGACACGGTGGTCGAACCAGTCGCCGGGTGGCTGGCGGCCGACGACCCGGAACCGTGGGTGGTGGAGACCTCCGGCTC
GACCGGTGCCCCGAAGCGGGTGGTCCTCCCGCGCGACGCCGTGCTCGCCTCGGTGCGCGCCTCCGCCCGCCGCGTGGGGG
CCACGGGCCAGTGGCTCCTCGCCCTGCCGCCGGCGTACGTCGCGGGCCTCCAGGTGGTCTGCCGGTCCCTGGCCGCCGGG
CACCCGCCGGTGCTGCTCGAGGACCACGGCTCCTTCGCGGCCGCGGCGGCCGCGATGGCGCCCGGCGAGCGGTTCGTCTC
GCTGGTCCCGACCCAGCTGCACCGGCTGCTGGAGACCGACGCGCCCGCGCTGGCGACGTTCCACACCGTGCTCCTCGGCG
GCGGTCCGATCGACCCGGCGCTGCGCCGCCGGGCAGCGGACGCCGGTGTCCACGTGATCGCGACGTACGGATCCGCGGAG
ACCGCCGGCGGCTGCGTCTACGACGGCGTCGCGCTCGACGGGGTCGCCGTCGCGCTCGACGCCGACGGCCGGGTCCGGAT
CGCGGGCCCGACGCTCTTCGCGGGGTACGCCGGCGACCCGGAGCTGACCGCCCGGGTCCTGGTCGACGGGTGGTTCCGCA
CCGCCGACGCCGGCCGGCTCGACGAGGACGGGCGGCTCCAGGTGCTCGGCCGGATCGACGACATGGTGGTCACCGGCGGC
GTGAACGTGCCCGCCCCCGCGGTCGCCGCCCGCCTGCGCGAGCACCCCGACGTCGAGGCGGCCGAGGTGCTCGGCGTCCC
CGACGAGGAGTGGGGCAACCGCGTGGTCGCGTTCGTGGTCGGCCCGGTCTCCCTCGACGTGGCGCGCGACTGGGTCGCCG
CGGCCCACCCGCGGTCCTGGGCGCCGCGCCAGCTCGTCGCCCTCGACACGATCCCCCTGCTCGGCAACGGCAAGCCGGAC
CGGTTGGCGCTGCGGCACGCTGCCGTCGAGGAGTGGGCATGA

Upstream 100 bases:

>100_bases
AGGGTCCGAGGCCGCGCGCCGGCGAGCCACTGGTCGGGAGTTGCCACGACCGGAGATTCAAGCATCAATCGCACCGTCTA
GCCTCAGCCAGGTGGACCGA

Downstream 100 bases:

>100_bases
TCGTCTGGTCGGTGCCGATGCGGACCCGGTTCCGCGGCATCACGGTCCGCGAGGGGGTGCTGCTGCGGGGCCCCGCCGAT
GCCGAGCGGCCGGGTTGGGG

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MDTVVEPVAGWLAADDPEPWVVETSGSTGAPKRVVLPRDAVLASVRASARRVGATGQWLLALPPAYVAGLQVVCRSLAAG
HPPVLLEDHGSFAAAAAAMAPGERFVSLVPTQLHRLLETDAPALATFHTVLLGGGPIDPALRRRAADAGVHVIATYGSAE
TAGGCVYDGVALDGVAVALDADGRVRIAGPTLFAGYAGDPELTARVLVDGWFRTADAGRLDEDGRLQVLGRIDDMVVTGG
VNVPAPAVAARLREHPDVEAAEVLGVPDEEWGNRVVAFVVGPVSLDVARDWVAAAHPRSWAPRQLVALDTIPLLGNGKPD
RLALRHAAVEEWA

Sequences:

>Translated_333_residues
MDTVVEPVAGWLAADDPEPWVVETSGSTGAPKRVVLPRDAVLASVRASARRVGATGQWLLALPPAYVAGLQVVCRSLAAG
HPPVLLEDHGSFAAAAAAMAPGERFVSLVPTQLHRLLETDAPALATFHTVLLGGGPIDPALRRRAADAGVHVIATYGSAE
TAGGCVYDGVALDGVAVALDADGRVRIAGPTLFAGYAGDPELTARVLVDGWFRTADAGRLDEDGRLQVLGRIDDMVVTGG
VNVPAPAVAARLREHPDVEAAEVLGVPDEEWGNRVVAFVVGPVSLDVARDWVAAAHPRSWAPRQLVALDTIPLLGNGKPD
RLALRHAAVEEWA
>Mature_333_residues
MDTVVEPVAGWLAADDPEPWVVETSGSTGAPKRVVLPRDAVLASVRASARRVGATGQWLLALPPAYVAGLQVVCRSLAAG
HPPVLLEDHGSFAAAAAAMAPGERFVSLVPTQLHRLLETDAPALATFHTVLLGGGPIDPALRRRAADAGVHVIATYGSAE
TAGGCVYDGVALDGVAVALDADGRVRIAGPTLFAGYAGDPELTARVLVDGWFRTADAGRLDEDGRLQVLGRIDDMVVTGG
VNVPAPAVAARLREHPDVEAAEVLGVPDEEWGNRVVAFVVGPVSLDVARDWVAAAHPRSWAPRQLVALDTIPLLGNGKPD
RLALRHAAVEEWA

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA). May be involved in the biosynthesis of menaquinone [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=154, Percent_Identity=34.4155844155844, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI187761343, Length=154, Percent_Identity=34.4155844155844, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI115511026, Length=144, Percent_Identity=34.7222222222222, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI156151445, Length=157, Percent_Identity=25.4777070063694, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI122937307, Length=260, Percent_Identity=26.1538461538462, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI145693145, Length=342, Percent_Identity=28.6549707602339, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1788595, Length=320, Percent_Identity=30.9375, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788107, Length=302, Percent_Identity=23.841059602649, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI32563687, Length=337, Percent_Identity=27.299703264095, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17558820, Length=297, Percent_Identity=28.956228956229, Blast_Score=101, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17559526, Length=351, Percent_Identity=24.7863247863248, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17557194, Length=185, Percent_Identity=29.1891891891892, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17538037, Length=162, Percent_Identity=29.6296296296296, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17560308, Length=107, Percent_Identity=33.6448598130841, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24581924, Length=350, Percent_Identity=25.4285714285714, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI20130357, Length=337, Percent_Identity=23.7388724035608, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI21355181, Length=149, Percent_Identity=29.5302013422819, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 34693; Mature: 34693

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTVVEPVAGWLAADDPEPWVVETSGSTGAPKRVVLPRDAVLASVRASARRVGATGQWLL
CCCHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEECCHHHHHHHHHHHHHHCCCCCCEEE
ALPPAYVAGLQVVCRSLAAGHPPVLLEDHGSFAAAAAAMAPGERFVSLVPTQLHRLLETD
ECCHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
APALATFHTVLLGGGPIDPALRRRAADAGVHVIATYGSAETAGGCVYDGVALDGVAVALD
CCHHHHHHHHHCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCEEECCEEECCEEEEEE
ADGRVRIAGPTLFAGYAGDPELTARVLVDGWFRTADAGRLDEDGRLQVLGRIDDMVVTGG
CCCEEEEECCEEEECCCCCHHHEEEEEEECCEECCCCCCCCCCCCEEEEECCCCEEEECC
VNVPAPAVAARLREHPDVEAAEVLGVPDEEWGNRVVAFVVGPVSLDVARDWVAAAHPRSW
CCCCHHHHHHHHHHCCCCCHHHHCCCCCHHHCCEEEEEEECCEEHHHHHHHHHHCCCCCC
APRQLVALDTIPLLGNGKPDRLALRHAAVEEWA
CCCEEEEEECEECCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MDTVVEPVAGWLAADDPEPWVVETSGSTGAPKRVVLPRDAVLASVRASARRVGATGQWLL
CCCHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEECCHHHHHHHHHHHHHHCCCCCCEEE
ALPPAYVAGLQVVCRSLAAGHPPVLLEDHGSFAAAAAAMAPGERFVSLVPTQLHRLLETD
ECCHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
APALATFHTVLLGGGPIDPALRRRAADAGVHVIATYGSAETAGGCVYDGVALDGVAVALD
CCHHHHHHHHHCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCEEECCEEECCEEEEEE
ADGRVRIAGPTLFAGYAGDPELTARVLVDGWFRTADAGRLDEDGRLQVLGRIDDMVVTGG
CCCEEEEECCEEEECCCCCHHHEEEEEEECCEECCCCCCCCCCCCEEEEECCCCEEEECC
VNVPAPAVAARLREHPDVEAAEVLGVPDEEWGNRVVAFVVGPVSLDVARDWVAAAHPRSW
CCCCHHHHHHHHHHCCCCCHHHHCCCCCHHHCCEEEEEEECCEEHHHHHHHHHHCCCCCC
APRQLVALDTIPLLGNGKPDRLALRHAAVEEWA
CCCEEEEEECEECCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]