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The map label for this gene is nth [H]

Identifier: 119714606

GI number: 119714606

Start: 362084

End: 362815

Strand: Direct

Name: nth [H]

Synonym: Noca_0340

Alternate gene names: 119714606

Gene position: 362084-362815 (Clockwise)

Preceding gene: 119714604

Following gene: 119714607

Centisome position: 7.26

GC content: 72.54

Gene sequence:

>732_bases
GTGCCTCGCCGTACCCTTGGGCCCGTGCCCGCAGTCCCCGAGTCCCGCACCGGTCTGGTCCGGCGCGCCCGCAAGATCGA
CCGGCTGCTCGCGGAGACCTACCCCGATGCCAGGTGCGAGCTGGACTTCGACAACCCCTTCGAGCTGCTCGTCGTCACCG
TGCTGTCGGCGCAGACCACCGACAAGCGGGTCAACGCGGTGCGGCCCACCCTGTTCGCGGCCTACCCCGACGCGAGGACG
ATGGCCGGCGCGGACCGCGCGACGCTCGAGGGCATCGTGGGGCCGTTGGGCTTCTTCCGTGCCAAGACCGAGTCGCTGCT
CAAGCTGAGCGCCGCGCTGGTCGAGCGCCACGGCGGCGAGGTGCCGCCCCGGCTCGACGACCTGGTGCAACTGCCCGGCG
TCGGGCGCAAGACCGCGAACGTCGTGCTCGGCAACGCCTTCGGCATCCCCGGCATCACCGTCGACACCCACTTCGGTCGG
CTGTCCCGCCGGTTCGCCTGGACGGAGGAGACCGACCCGGTCAAGGTCGAGCACGCCGTCGGCGCCCTGTTCGAGAAGCG
CGACTGGACGATGCTCAGCCACCACCTGATCTGGCACGGTCGGCGGATCTGCCACGCCCGCAACCCCGCCTGTGGGGCGT
GCCCGGTCGCGCGCTGGTGCCCGGCGTACGGCGAGGGGCCGACCGACCCGGCCGCCGCGGCGAAGCTGGTGAAGACCGAG
GGCAGGGCATGA

Upstream 100 bases:

>100_bases
ACGGGTTCCGCATTGATCCTATCCAGTGGAAAAGGTCACAGGAACAACCGTCCGGCGTATGTCGGGGTGCGCTGTCCGGT
CACCGACCACCGGGTGGTCG

Downstream 100 bases:

>100_bases
GGCGACTCCTCCCGGCCGTGCTCGCCGCGGCCGTGCTGGTGCTGGGCGCGTGCGGCTCCGCGGACCTGCCGAAACCACCC
GGCCAGGCCAAGGTCGACGT

Product: endonuclease III / DNA-(apurinic or apyrimidinic site) lyase

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MPRRTLGPVPAVPESRTGLVRRARKIDRLLAETYPDARCELDFDNPFELLVVTVLSAQTTDKRVNAVRPTLFAAYPDART
MAGADRATLEGIVGPLGFFRAKTESLLKLSAALVERHGGEVPPRLDDLVQLPGVGRKTANVVLGNAFGIPGITVDTHFGR
LSRRFAWTEETDPVKVEHAVGALFEKRDWTMLSHHLIWHGRRICHARNPACGACPVARWCPAYGEGPTDPAAAAKLVKTE
GRA

Sequences:

>Translated_243_residues
MPRRTLGPVPAVPESRTGLVRRARKIDRLLAETYPDARCELDFDNPFELLVVTVLSAQTTDKRVNAVRPTLFAAYPDART
MAGADRATLEGIVGPLGFFRAKTESLLKLSAALVERHGGEVPPRLDDLVQLPGVGRKTANVVLGNAFGIPGITVDTHFGR
LSRRFAWTEETDPVKVEHAVGALFEKRDWTMLSHHLIWHGRRICHARNPACGACPVARWCPAYGEGPTDPAAAAKLVKTE
GRA
>Mature_242_residues
PRRTLGPVPAVPESRTGLVRRARKIDRLLAETYPDARCELDFDNPFELLVVTVLSAQTTDKRVNAVRPTLFAAYPDARTM
AGADRATLEGIVGPLGFFRAKTESLLKLSAALVERHGGEVPPRLDDLVQLPGVGRKTANVVLGNAFGIPGITVDTHFGRL
SRRFAWTEETDPVKVEHAVGALFEKRDWTMLSHHLIWHGRRICHARNPACGACPVARWCPAYGEGPTDPAAAAKLVKTEG
RA

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=179, Percent_Identity=31.8435754189944, Blast_Score=100, Evalue=1e-21,
Organism=Escherichia coli, GI1787920, Length=206, Percent_Identity=39.3203883495146, Blast_Score=136, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17554540, Length=195, Percent_Identity=34.3589743589744, Blast_Score=107, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6324530, Length=213, Percent_Identity=27.6995305164319, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI45550361, Length=179, Percent_Identity=29.608938547486, Blast_Score=89, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 26573; Mature: 26442

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRRTLGPVPAVPESRTGLVRRARKIDRLLAETYPDARCELDFDNPFELLVVTVLSAQTT
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHCCCC
DKRVNAVRPTLFAAYPDARTMAGADRATLEGIVGPLGFFRAKTESLLKLSAALVERHGGE
HHHHHHHCCCEEEECCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VPPRLDDLVQLPGVGRKTANVVLGNAFGIPGITVDTHFGRLSRRFAWTEETDPVKVEHAV
CCCCHHHHHHCCCCCCCCCEEEEECCCCCCCEEEECHHHHHHHHCCCCCCCCCEEHHHHH
GALFEKRDWTMLSHHLIWHGRRICHARNPACGACPVARWCPAYGEGPTDPAAAAKLVKTE
HHHHHHCCHHHHHHHHHHHCHHHCCCCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHCC
GRA
CCC
>Mature Secondary Structure 
PRRTLGPVPAVPESRTGLVRRARKIDRLLAETYPDARCELDFDNPFELLVVTVLSAQTT
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHCCCC
DKRVNAVRPTLFAAYPDARTMAGADRATLEGIVGPLGFFRAKTESLLKLSAALVERHGGE
HHHHHHHCCCEEEECCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VPPRLDDLVQLPGVGRKTANVVLGNAFGIPGITVDTHFGRLSRRFAWTEETDPVKVEHAV
CCCCHHHHHHCCCCCCCCCEEEEECCCCCCCEEEECHHHHHHHHCCCCCCCCCEEHHHHH
GALFEKRDWTMLSHHLIWHGRRICHARNPACGACPVARWCPAYGEGPTDPAAAAKLVKTE
HHHHHHCCHHHHHHHHHHHCHHHCCCCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHCC
GRA
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]