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The map label for this gene is yerD [H]

Identifier: 119714546

GI number: 119714546

Start: 299702

End: 301321

Strand: Reverse

Name: yerD [H]

Synonym: Noca_0279

Alternate gene names: 119714546

Gene position: 301321-299702 (Counterclockwise)

Preceding gene: 119714551

Following gene: 119714542

Centisome position: 6.04

GC content: 72.35

Gene sequence:

>1620_bases
ATGAGGAAGACGACGCTCGCCGCGCTCCCCGCTGCTGCTCTCGGGGCGGTCGCCGCCCACGACCTGCTGCAACGACGCCA
CGCCATCCTGCGCAACTTCCCGGTGGTCGGGCACGCGCGGTACTGGCTGGAGGCGCTCGGACCGGAGCTGCGGCAGTACA
TCGTCGCGAGCAACGACGAGGAGCGGCCCTTCAGCCGGGACCAGCGGCGCTGGGTCTACGCCTCGGCGAAGCTGCAGAAC
AACTACTTCGGGTTCGGCACCGACAACGACCTCGAGCACAGCTCCGGCAACGTGATCGTCCATCACCGCACGTTCGGGCG
CTCCTACCCGACCCACGGCGCGGTCGGTCAGGAGGCTCGGCTGCCCTCGGCGAAGATCCTCGGCGGCCCGCGCGGGCGCC
GGCGGGCGTTCCGGCCGGCGTCGGTCGTCAACGTGTCGGGCATGAGCTTCGGGTCGCTGTCGGGCAACGCCGTCGAAGCG
ATCAACCGGGGCGCGGCCGAGGCCGGGTGCCTGCACAACACCGGCGAGGGCGCGGTGTCGCCGTACCACCGCCAGGGCGC
CGACCTGGTCTTCCAGATCGGTACGGCGTACTTCGGCTGCCGCGACGACGACGGCAGGTTCAGCCTGCCGCGGCTCAAGG
ACCTCGTGGAGTCGGCGCCGGTCCGGGCCCTGGAGATCAAGCTCAGCCAGGGCGCCAAACCCGGGCTCGGCGGCGTCCTG
CCGGCCGCCAAGGTGTCGGCGGAGATCGCCGCGACGCGCGGGGTCCCGGCCGGTCTCGACTGCCTGAGCCCGTCGCGGCA
CGCGGAGTTCGACGACGTGGACTCGCTGCTCGACTTCGTCGAGCTGCTCGCGGACGAGACCGGGCTGCCGGTCGGGATCA
AGTCGGCGGTGGGCGACCTCGGGTTCTGGCGAGACCTCACCGAGGAGATGGCCAAGGGCCAGCGCGGCGTCGACTTCGTC
ACGATCGACGGCGGCGAGGGCGGGACCGGAGCCTCCCCGCTGATCTTCACCGACGCGGTGTCACTGCCGTTCCGGCTCGG
GTTCGCGCGCGTGTACGCCGAGTTCGCCCGGCGCGGCCTCCACGAGGACGTGACCTTCATCGGCTCCGGCAAGCTCGGGC
TGCCCGACAACGCGGTCGTCGCCTTCGCCCTGGGCGCCGACCTGGTCAACGTGGCCCGTGAGGCGATGCTCGCCGTCGGG
TGCATCCAGGCGCAGAAGTGCCATACCGGCGAGTGCCCGACCGGCGTCGCGACCCAGAACCCGTGGTTGGCCCACGGCCT
CGACCCGGTCGAGAAGTCCGGACGCGTCGCCAACTACGTGCGCACCCTGCGGCGCGACCTGCTCAAGGTCGCCGAGACCT
GCGGGGTCGAGCACCCGGGGCTGATCGGCCCCCACGACGTCGAGATCCTCGACAACCTCTCCGACGGCCGGCTCCTCGAC
GAGGTGTACGGCTACGACCCCGGGTGGGGGTTCCCGTCGGCGCGGGACCGGCAGGAGATCGCCGCCATCATGTCCGCGAC
CGAGGAGCCCGAGACCCGCACCGAAGGTCCGCCGGAGACCGCCGAGGAGGGCGCGCCCGGCACCGAGCACGCCGGCGACG
AGGAGCCGAGCGGCGACTGA

Upstream 100 bases:

>100_bases
GGTCGCGTCGACGTGGGAGCTGACGTTTCACCCCGACATCATCGCCGAAGCGGCTCCCGAGGTCCCGACTCGCCCGCTGC
CGGTTGGGTACTGCCCCGAC

Downstream 100 bases:

>100_bases
CCGGCTCGACCGAACGCCTCCGCGAGGTCAGGACGGCCGGACGACCTGCTGGAGCGCCCACCGGTTGCCGTCGGGGTCCT
GGAAGTACACGAAGCGCCCC

Product: glutamate synthase (ferredoxin)

Products: L-glutamine; 2-oxoglutarate; reduced ferredoxin

Alternate protein names: NA

Number of amino acids: Translated: 539; Mature: 539

Protein sequence:

>539_residues
MRKTTLAALPAAALGAVAAHDLLQRRHAILRNFPVVGHARYWLEALGPELRQYIVASNDEERPFSRDQRRWVYASAKLQN
NYFGFGTDNDLEHSSGNVIVHHRTFGRSYPTHGAVGQEARLPSAKILGGPRGRRRAFRPASVVNVSGMSFGSLSGNAVEA
INRGAAEAGCLHNTGEGAVSPYHRQGADLVFQIGTAYFGCRDDDGRFSLPRLKDLVESAPVRALEIKLSQGAKPGLGGVL
PAAKVSAEIAATRGVPAGLDCLSPSRHAEFDDVDSLLDFVELLADETGLPVGIKSAVGDLGFWRDLTEEMAKGQRGVDFV
TIDGGEGGTGASPLIFTDAVSLPFRLGFARVYAEFARRGLHEDVTFIGSGKLGLPDNAVVAFALGADLVNVAREAMLAVG
CIQAQKCHTGECPTGVATQNPWLAHGLDPVEKSGRVANYVRTLRRDLLKVAETCGVEHPGLIGPHDVEILDNLSDGRLLD
EVYGYDPGWGFPSARDRQEIAAIMSATEEPETRTEGPPETAEEGAPGTEHAGDEEPSGD

Sequences:

>Translated_539_residues
MRKTTLAALPAAALGAVAAHDLLQRRHAILRNFPVVGHARYWLEALGPELRQYIVASNDEERPFSRDQRRWVYASAKLQN
NYFGFGTDNDLEHSSGNVIVHHRTFGRSYPTHGAVGQEARLPSAKILGGPRGRRRAFRPASVVNVSGMSFGSLSGNAVEA
INRGAAEAGCLHNTGEGAVSPYHRQGADLVFQIGTAYFGCRDDDGRFSLPRLKDLVESAPVRALEIKLSQGAKPGLGGVL
PAAKVSAEIAATRGVPAGLDCLSPSRHAEFDDVDSLLDFVELLADETGLPVGIKSAVGDLGFWRDLTEEMAKGQRGVDFV
TIDGGEGGTGASPLIFTDAVSLPFRLGFARVYAEFARRGLHEDVTFIGSGKLGLPDNAVVAFALGADLVNVAREAMLAVG
CIQAQKCHTGECPTGVATQNPWLAHGLDPVEKSGRVANYVRTLRRDLLKVAETCGVEHPGLIGPHDVEILDNLSDGRLLD
EVYGYDPGWGFPSARDRQEIAAIMSATEEPETRTEGPPETAEEGAPGTEHAGDEEPSGD
>Mature_539_residues
MRKTTLAALPAAALGAVAAHDLLQRRHAILRNFPVVGHARYWLEALGPELRQYIVASNDEERPFSRDQRRWVYASAKLQN
NYFGFGTDNDLEHSSGNVIVHHRTFGRSYPTHGAVGQEARLPSAKILGGPRGRRRAFRPASVVNVSGMSFGSLSGNAVEA
INRGAAEAGCLHNTGEGAVSPYHRQGADLVFQIGTAYFGCRDDDGRFSLPRLKDLVESAPVRALEIKLSQGAKPGLGGVL
PAAKVSAEIAATRGVPAGLDCLSPSRHAEFDDVDSLLDFVELLADETGLPVGIKSAVGDLGFWRDLTEEMAKGQRGVDFV
TIDGGEGGTGASPLIFTDAVSLPFRLGFARVYAEFARRGLHEDVTFIGSGKLGLPDNAVVAFALGADLVNVAREAMLAVG
CIQAQKCHTGECPTGVATQNPWLAHGLDPVEKSGRVANYVRTLRRDLLKVAETCGVEHPGLIGPHDVEILDNLSDGRLLD
EVYGYDPGWGFPSARDRQEIAAIMSATEEPETRTEGPPETAEEGAPGTEHAGDEEPSGD

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0069

COG function: function code E; Glutamate synthase domain 2

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI308199519, Length=252, Percent_Identity=32.1428571428571, Blast_Score=95, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17570289, Length=305, Percent_Identity=32.4590163934426, Blast_Score=122, Evalue=7e-28,
Organism=Saccharomyces cerevisiae, GI6320030, Length=322, Percent_Identity=29.8136645962733, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI28574881, Length=335, Percent_Identity=29.2537313432836, Blast_Score=115, Evalue=7e-26,
Organism=Drosophila melanogaster, GI24665539, Length=335, Percent_Identity=29.2537313432836, Blast_Score=115, Evalue=7e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR002932 [H]

Pfam domain/function: PF01645 Glu_synthase [H]

EC number: 1.4.7.1

Molecular weight: Translated: 57539; Mature: 57539

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKTTLAALPAAALGAVAAHDLLQRRHAILRNFPVVGHARYWLEALGPELRQYIVASNDE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCHHHHHHHHCCCCC
ERPFSRDQRRWVYASAKLQNNYFGFGTDNDLEHSSGNVIVHHRTFGRSYPTHGAVGQEAR
CCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCC
LPSAKILGGPRGRRRAFRPASVVNVSGMSFGSLSGNAVEAINRGAAEAGCLHNTGEGAVS
CCCHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHCCCHHHCCCCCCCCCCCC
PYHRQGADLVFQIGTAYFGCRDDDGRFSLPRLKDLVESAPVRALEIKLSQGAKPGLGGVL
CHHHCCCCEEEEECCCEECCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCC
PAAKVSAEIAATRGVPAGLDCLSPSRHAEFDDVDSLLDFVELLADETGLPVGIKSAVGDL
CHHHHHHHHHHHCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHH
GFWRDLTEEMAKGQRGVDFVTIDGGEGGTGASPLIFTDAVSLPFRLGFARVYAEFARRGL
HHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCEEEECCHHCHHHHHHHHHHHHHHHCCC
HEDVTFIGSGKLGLPDNAVVAFALGADLVNVAREAMLAVGCIQAQKCHTGECPTGVATQN
CCCCEEEECCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
PWLAHGLDPVEKSGRVANYVRTLRRDLLKVAETCGVEHPGLIGPHDVEILDNLSDGRLLD
CCHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCHHH
EVYGYDPGWGFPSARDRQEIAAIMSATEEPETRTEGPPETAEEGAPGTEHAGDEEPSGD
HHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRKTTLAALPAAALGAVAAHDLLQRRHAILRNFPVVGHARYWLEALGPELRQYIVASNDE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCHHHHHHHHCCCCC
ERPFSRDQRRWVYASAKLQNNYFGFGTDNDLEHSSGNVIVHHRTFGRSYPTHGAVGQEAR
CCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCC
LPSAKILGGPRGRRRAFRPASVVNVSGMSFGSLSGNAVEAINRGAAEAGCLHNTGEGAVS
CCCHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHCCCHHHCCCCCCCCCCCC
PYHRQGADLVFQIGTAYFGCRDDDGRFSLPRLKDLVESAPVRALEIKLSQGAKPGLGGVL
CHHHCCCCEEEEECCCEECCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCC
PAAKVSAEIAATRGVPAGLDCLSPSRHAEFDDVDSLLDFVELLADETGLPVGIKSAVGDL
CHHHHHHHHHHHCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHH
GFWRDLTEEMAKGQRGVDFVTIDGGEGGTGASPLIFTDAVSLPFRLGFARVYAEFARRGL
HHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCEEEECCHHCHHHHHHHHHHHHHHHCCC
HEDVTFIGSGKLGLPDNAVVAFALGADLVNVAREAMLAVGCIQAQKCHTGECPTGVATQN
CCCCEEEECCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
PWLAHGLDPVEKSGRVANYVRTLRRDLLKVAETCGVEHPGLIGPHDVEILDNLSDGRLLD
CCHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCHHH
EVYGYDPGWGFPSARDRQEIAAIMSATEEPETRTEGPPETAEEGAPGTEHAGDEEPSGD
HHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-glutamate; oxidized ferredoxin

Specific reaction: 2 L-glutamate + 2 oxidized ferredoxin = L-glutamine + 2-oxoglutarate + 2 reduced ferredoxin

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9701819; 9384377; 8969499 [H]