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The map label for this gene is ligT [C]

Identifier: 119714540

GI number: 119714540

Start: 295784

End: 296350

Strand: Direct

Name: ligT [C]

Synonym: Noca_0273

Alternate gene names: 119714540

Gene position: 295784-296350 (Clockwise)

Preceding gene: 119714539

Following gene: 119714543

Centisome position: 5.93

GC content: 76.9

Gene sequence:

>567_bases
GTGCGGATGTTCGTGGCGCTGGTGCCGCCGGCCGAGGCGGTCGAGCACCTGGACGCGTTCCTCGAGCCGCGGCGCTCGGC
TGCGGCGTTCCGGTGGGCCGGTGTCGAGCAGCTGCACGTGACTGTGGCGTTCCTGGCCGAGGTCGAGGAGCGCCGGCTCG
AGGACCTGGTCGAGCGGCTCGGGCGCGCGGCGGCCCGCCGGTCCGCGTTCGACACCGCGATCACGGGTGGCGGCGCGTTC
CCGAACGTGGGGCGGGCCCGGGTGCTCTGGGCGGGCCTCGCGCTCGATGAGCACGGCCGCACCCAGCTCGACCGACTCGC
CACCGGCTGTCGGACCGCCGCCAACCGGGCCGGCGTCCCGGTCGACGGGCAGCGCTTCCGGCCGCACCTCACCGTCGCCA
GGATCGCGCACCCCACGGAGGCGACCAGCTGGGTCCGGCTCCTCGACGGGTACGCCGGACCCGCATGGCGGGCCGACCGG
GTCGAGCTGGTGGCGTCGTACCTCGGCGAGGGCCCGCGCGGGCGGCCGCGCTACGAGACGGTCGCCGAGCTGGAGCTCGC
CGGGTAG

Upstream 100 bases:

>100_bases
ACGGGGTTGACCGTCCGACAATGTGGTTGCGGTCGGCGCTTGGCTGCGGACCGATGCTTGCGGACCCGATGCCTGTAGAG
GGGCCGACTAGGTTGAGCGG

Downstream 100 bases:

>100_bases
CCGGCGTCAGGCCGTCTCGGGGGTGGCGCCCAGCTTGCGCAGCGCCTCCCGCAGGGCGGCGTCGGGCCGCTCTCCGCCGT
CGCGGAGCTGAGCGAGCACG

Product: 2'-5' RNA ligase

Products: NA

Alternate protein names: 2 5' RNA Ligase; tRNA Ligase

Number of amino acids: Translated: 188; Mature: 188

Protein sequence:

>188_residues
MRMFVALVPPAEAVEHLDAFLEPRRSAAAFRWAGVEQLHVTVAFLAEVEERRLEDLVERLGRAAARRSAFDTAITGGGAF
PNVGRARVLWAGLALDEHGRTQLDRLATGCRTAANRAGVPVDGQRFRPHLTVARIAHPTEATSWVRLLDGYAGPAWRADR
VELVASYLGEGPRGRPRYETVAELELAG

Sequences:

>Translated_188_residues
MRMFVALVPPAEAVEHLDAFLEPRRSAAAFRWAGVEQLHVTVAFLAEVEERRLEDLVERLGRAAARRSAFDTAITGGGAF
PNVGRARVLWAGLALDEHGRTQLDRLATGCRTAANRAGVPVDGQRFRPHLTVARIAHPTEATSWVRLLDGYAGPAWRADR
VELVASYLGEGPRGRPRYETVAELELAG
>Mature_188_residues
MRMFVALVPPAEAVEHLDAFLEPRRSAAAFRWAGVEQLHVTVAFLAEVEERRLEDLVERLGRAAARRSAFDTAITGGGAF
PNVGRARVLWAGLALDEHGRTQLDRLATGCRTAANRAGVPVDGQRFRPHLTVARIAHPTEATSWVRLLDGYAGPAWRADR
VELVASYLGEGPRGRPRYETVAELELAG

Specific function: Specifically Ligates tRNA Half-Molecules Containing Nucleoside Base Modifications And Shows A Preference Among Different tRNA Species. Acts In The Absence Of ATP To Form A 2'-5' Phosphodiester Linkage. [C]

COG id: COG1514

COG function: function code J; 2'-5' RNA ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.5.1.- [C]

Molecular weight: Translated: 20588; Mature: 20588

Theoretical pI: Translated: 8.52; Mature: 8.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMFVALVPPAEAVEHLDAFLEPRRSAAAFRWAGVEQLHVTVAFLAEVEERRLEDLVERL
CCEEEEECCCHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GRAAARRSAFDTAITGGGAFPNVGRARVLWAGLALDEHGRTQLDRLATGCRTAANRAGVP
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCEECCCCHHHHHHHHHHHHHHHHCCCCC
VDGQRFRPHLTVARIAHPTEATSWVRLLDGYAGPAWRADRVELVASYLGEGPRGRPRYET
CCCCCCCCCHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHH
VAELELAG
HHHEEECC
>Mature Secondary Structure
MRMFVALVPPAEAVEHLDAFLEPRRSAAAFRWAGVEQLHVTVAFLAEVEERRLEDLVERL
CCEEEEECCCHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GRAAARRSAFDTAITGGGAFPNVGRARVLWAGLALDEHGRTQLDRLATGCRTAANRAGVP
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCEECCCCHHHHHHHHHHHHHHHHCCCCC
VDGQRFRPHLTVARIAHPTEATSWVRLLDGYAGPAWRADRVELVASYLGEGPRGRPRYET
CCCCCCCCCHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHH
VAELELAG
HHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA