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The map label for this gene is bcpA [H]

Identifier: 119714471

GI number: 119714471

Start: 219141

End: 220118

Strand: Reverse

Name: bcpA [H]

Synonym: Noca_0204

Alternate gene names: 119714471

Gene position: 220118-219141 (Counterclockwise)

Preceding gene: 119714472

Following gene: 119714470

Centisome position: 4.41

GC content: 74.23

Gene sequence:

>978_bases
GTGAACAGCAGCAGCCACCAGGAGTTCCCGCACCTGGCCGACCTGGTCGGCGTCGGCCCCGCGCCGACCGCGCACGACCG
GCGTACGGCGCTGCGCACCGCACTGCGAGAGGCCACGGCCGGCGCCGGACCGCTGCTGCTCCCGGGGGTCACTGACGCCC
TCGGTGCGCGGCTCGTCGAGGCCGCGGGCTTCGGCGCGGCCTATGCGACCGGGGCCGGGCTCGCCAATGCGCAGTACGGG
CTGCCCGACCTCGGCCTGGTCTCCCTCGGCGAGGTCGCCGACCACGTCGGCCGCATCACCGAGGCCACCAGGCTGCCGGT
CGTCGTCGACGCGGACACCGGGTACGGCGGTCCGCTGGCGGCGATGCGCACGATGCGGCTGCTCGAACGCGCCGGGGCTG
CCGGCATCCAGCTCGAGGACCAGGAGATGCCCAAGCGGTGCGGGCACTTCGACTCCCACACGCTGATCCCGCTCGGGCAC
ATGCAGGCCAAGATCGCCGCGGTCCTCGACGCCCGCGAGGACGACGCGACGGTGCTCGTCGCACGCACCGACGCCCGCAG
CGCCGAGGGGATCGACCGGGCGGTCGAGCGGGCGCGCGCCTACGTCGAAGCGGGCGCGGACGTGATCTTCGTCGAGGCGC
CGCGCACGGTCGCGGAGCTGACGCTGGTCGGCCGCGAGCTGGCCGGCACCCCCCTGGTCGTCAACGTCGTCGAGGGCGGC
AAGACCCCACACCTCAGCGCGCAGGAGTACGCCGACCTCGGCTTCACCGTCGTGCTGCACGCCAACTACCTGATGCGGTC
GATGATGTCGGCCGGCCGCGCCGCGCTCGCCCACCTGGCGGCCGCCGGCGAGACCGTGACCCGGGCCGAGCAGATGGCGA
CCTGGAGCGAGCGTCAGTCGCTGTTCCACCTCCCGGCGTTCACCGCCGCCGAGGCCTACTTCGACCAGCCCCTCGACGTG
CTTCGGAGCGAGCGGTGA

Upstream 100 bases:

>100_bases
ATTCTCCGGGGGTTCGGGGTTGCGGAGATCGCGCCGGGGTGCAACCCTGCTCTCTGTAACGATCACTACATGGAAGCCAC
CGGATCGGAGCCACTCGCCC

Downstream 100 bases:

>100_bases
TCCCCGGCCTGCCGATCGAGGGCGAGCTGCCCGACCGGGTCGGTGTACTCGTGCTCGGCAGCGGGCTCGCCGGGTGCGCC
GCCCTGCTCGCCGCGGCTGA

Product: 2,3-dimethylmalate lyase

Products: NA

Alternate protein names: Carboxyphosphonoenolpyruvate phosphonomutase; CPEP phosphonomutase [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MNSSSHQEFPHLADLVGVGPAPTAHDRRTALRTALREATAGAGPLLLPGVTDALGARLVEAAGFGAAYATGAGLANAQYG
LPDLGLVSLGEVADHVGRITEATRLPVVVDADTGYGGPLAAMRTMRLLERAGAAGIQLEDQEMPKRCGHFDSHTLIPLGH
MQAKIAAVLDAREDDATVLVARTDARSAEGIDRAVERARAYVEAGADVIFVEAPRTVAELTLVGRELAGTPLVVNVVEGG
KTPHLSAQEYADLGFTVVLHANYLMRSMMSAGRAALAHLAAAGETVTRAEQMATWSERQSLFHLPAFTAAEAYFDQPLDV
LRSER

Sequences:

>Translated_325_residues
MNSSSHQEFPHLADLVGVGPAPTAHDRRTALRTALREATAGAGPLLLPGVTDALGARLVEAAGFGAAYATGAGLANAQYG
LPDLGLVSLGEVADHVGRITEATRLPVVVDADTGYGGPLAAMRTMRLLERAGAAGIQLEDQEMPKRCGHFDSHTLIPLGH
MQAKIAAVLDAREDDATVLVARTDARSAEGIDRAVERARAYVEAGADVIFVEAPRTVAELTLVGRELAGTPLVVNVVEGG
KTPHLSAQEYADLGFTVVLHANYLMRSMMSAGRAALAHLAAAGETVTRAEQMATWSERQSLFHLPAFTAAEAYFDQPLDV
LRSER
>Mature_325_residues
MNSSSHQEFPHLADLVGVGPAPTAHDRRTALRTALREATAGAGPLLLPGVTDALGARLVEAAGFGAAYATGAGLANAQYG
LPDLGLVSLGEVADHVGRITEATRLPVVVDADTGYGGPLAAMRTMRLLERAGAAGIQLEDQEMPKRCGHFDSHTLIPLGH
MQAKIAAVLDAREDDATVLVARTDARSAEGIDRAVERARAYVEAGADVIFVEAPRTVAELTLVGRELAGTPLVVNVVEGG
KTPHLSAQEYADLGFTVVLHANYLMRSMMSAGRAALAHLAAAGETVTRAEQMATWSERQSLFHLPAFTAAEAYFDQPLDV
LRSER

Specific function: Catalyzes the formation of an unusual C-P bond that is involved in the biosynthesis of the antibiotic bialaphos (BA). Catalyzes the rearrangement of the carboxyphosphono group of CPEP to form the C-P bond of phosphinopyruvate [H]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily [H]

Homologues:

Organism=Escherichia coli, GI1786525, Length=268, Percent_Identity=36.5671641791045, Blast_Score=158, Evalue=5e-40,
Organism=Saccharomyces cerevisiae, GI6325263, Length=194, Percent_Identity=28.8659793814433, Blast_Score=70, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012697
- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =2.7.8.23 [H]

Molecular weight: Translated: 34230; Mature: 34230

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSSSHQEFPHLADLVGVGPAPTAHDRRTALRTALREATAGAGPLLLPGVTDALGARLVE
CCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHH
AAGFGAAYATGAGLANAQYGLPDLGLVSLGEVADHVGRITEATRLPVVVDADTGYGGPLA
HHCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHH
AMRTMRLLERAGAAGIQLEDQEMPKRCGHFDSHTLIPLGHMQAKIAAVLDAREDDATVLV
HHHHHHHHHHCCCCCCEECHHHHHHHCCCCCCCCEEECHHHHHHHHHHHCCCCCCCEEEE
ARTDARSAEGIDRAVERARAYVEAGADVIFVEAPRTVAELTLVGRELAGTPLVVNVVEGG
EECCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCCEEEEEECCC
KTPHLSAQEYADLGFTVVLHANYLMRSMMSAGRAALAHLAAAGETVTRAEQMATWSERQS
CCCCCCHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
LFHLPAFTAAEAYFDQPLDVLRSER
EECCCCHHHHHHHHCCCHHHHCCCC
>Mature Secondary Structure
MNSSSHQEFPHLADLVGVGPAPTAHDRRTALRTALREATAGAGPLLLPGVTDALGARLVE
CCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHH
AAGFGAAYATGAGLANAQYGLPDLGLVSLGEVADHVGRITEATRLPVVVDADTGYGGPLA
HHCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHH
AMRTMRLLERAGAAGIQLEDQEMPKRCGHFDSHTLIPLGHMQAKIAAVLDAREDDATVLV
HHHHHHHHHHCCCCCCEECHHHHHHHCCCCCCCCEEECHHHHHHHHHHHCCCCCCCEEEE
ARTDARSAEGIDRAVERARAYVEAGADVIFVEAPRTVAELTLVGRELAGTPLVVNVVEGG
EECCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCCEEEEEECCC
KTPHLSAQEYADLGFTVVLHANYLMRSMMSAGRAALAHLAAAGETVTRAEQMATWSERQS
CCCCCCHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
LFHLPAFTAAEAYFDQPLDVLRSER
EECCCCHHHHHHHHCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2160937; 1320191; 1330557; 3611020 [H]