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The map label for this gene is dagK [H]

Identifier: 119714440

GI number: 119714440

Start: 185796

End: 187316

Strand: Reverse

Name: dagK [H]

Synonym: Noca_0173

Alternate gene names: 119714440

Gene position: 187316-185796 (Counterclockwise)

Preceding gene: 119714446

Following gene: 119714437

Centisome position: 3.76

GC content: 72.12

Gene sequence:

>1521_bases
ATGTTCGTCCTGGTCACCATCGCCGTGCAGACCGGCTGGGGACCGGTGACCTCGATCGACAACCGGGGCCAGGGCGCCGA
GACCTGGGCCGTCGACGAGCGCTGGCTGCGCCTGCCGCTGCGCTGGATCGAGGTCGGCTTCGGCGCCATCGGCACCTGGG
TGATCGTCGGCGTGCTCGCCGTGCTGCTGTTCGTGCGGCAGCACCGACGCGCCGCGGTGTACGCCGTCGGCGTGGTGGCG
GTCACCAAGATCGCCACCACGATCCTCAAGGCCGTGTTCGGGCGGCACCGTCCCGACTGGCAGAACCCCGAGCACCTGCT
GGACAGCAACGCCTACCCCAGCGGTCACTCCTCGACGATCGTCGCGCTCGTCGGGGTCGCCGCGGTGCTGGTGATGATGC
TGGTCCGGCGGCGCAGCGCCCGGCACACGGCGTACGCCCTCCTCGCCGTGCTGGCGGTCGTGGTGGGCCTCGACCGGGTG
CTGCTCGGTCGGCACTTCCCGAGCGACGTGGTCGGCGGGGCCCTCCTCGGCGCGATCGTCGTCCTGCTCGGGATCGTCGT
CCAGAACCCGCTCCCCCGTAGCCATGCCGCCAGCGTCGAGCCGCTGCCCGAGGTCTTCGGGTCCGAGCACCGGCTGACGG
TGATCCTCAACCCGGTCAAGGTGGAGGACGTCGGCCAGTTCGAGGCGCTGGTCACCGCGATGGCCGCCGAGGCCGGCTGG
TCCGCACCGACCTGGCACTACACGACCGTCGAGGACCCCGGCACCGGGATGGCCGAGGCCGCCGCGGTCGCCGGCACCGA
CCTGGTACTCGTGTGCGGCGGCGACGGCACCGTGCGCGAGGTCTGCGCGGAGCTGGCCGGCACCGGGATCCCGGTCGGGA
TCATCCCGGCGGGCACCGGCAACCTGCTGGCCCGCAACCTCGACATCCCGCTCTACCTGCGGGCGGCGATCGACGTCGCC
CTCACCGGTCAGGACCGCGCGATCGACCTGGTGGAGGTCTCCGGCGACGGCTTCGAGGACACCCACTTCATGGTGATGGC
CGGGATGGGCTTCGACGCCGCGATCATGGAGGGCGTCAACGAGGACATCAAGAAGCGGGTCGGCTGGCTGGCCTACGTGC
TCTCCGCGCTCAAGTCGCTGATGTTCCCCGCCGTCCGGGTCGAGGTCTCGGTCGACGGCGGCGAGTTCACCAGGCACCGG
GCCCGCACGATCGTGGTCGGGAACGTCGGCTTCCTCCAGGCCGGGATGCCGCTGCTGCCGGACGCCGCGATCGACGACGG
CGTCCTCGACGTGGTGATGCTGCACCCGCGGCGGTTCCTGTCGTGGATCCCGCTGGCCTACCGGGTGCTGGCCCGGCGCC
CGCGCACCGACGAGCTCGTGAACCGGATGACCGGGCACACCGTCGTCGTCCGCGCCTCCACCGACACCCCCCGCCAGCTC
GACGGCGACTCGATCGGCCCCGGCCGCGAGCTCCGGATGACCTGTGTCCACGGCCGGCTGCTGGTCCGAGTGCCCCGCTA
G

Upstream 100 bases:

>100_bases
TCGCACCGATTCCTCAGCCGGCGCCCGGCATGGAAAACTCAGGCCCGTGCTCGACCGCCCCCGCGCCACCCTGCTCACCT
GGACGGCGGCGCTCCTCGTC

Downstream 100 bases:

>100_bases
CCGCGGCTGGCGTGTCGGGGTGATCACCCGACGATGTGGTTTCGGTGGGCGCACAGGCTGCGGGCCGCCGCCCCGGGACG
CATCGTGCTGCTTCAGTGCC

Product: PA-phosphatase-like phosphoesterase

Products: NA

Alternate protein names: DAG kinase; DAGK [H]

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MFVLVTIAVQTGWGPVTSIDNRGQGAETWAVDERWLRLPLRWIEVGFGAIGTWVIVGVLAVLLFVRQHRRAAVYAVGVVA
VTKIATTILKAVFGRHRPDWQNPEHLLDSNAYPSGHSSTIVALVGVAAVLVMMLVRRRSARHTAYALLAVLAVVVGLDRV
LLGRHFPSDVVGGALLGAIVVLLGIVVQNPLPRSHAASVEPLPEVFGSEHRLTVILNPVKVEDVGQFEALVTAMAAEAGW
SAPTWHYTTVEDPGTGMAEAAAVAGTDLVLVCGGDGTVREVCAELAGTGIPVGIIPAGTGNLLARNLDIPLYLRAAIDVA
LTGQDRAIDLVEVSGDGFEDTHFMVMAGMGFDAAIMEGVNEDIKKRVGWLAYVLSALKSLMFPAVRVEVSVDGGEFTRHR
ARTIVVGNVGFLQAGMPLLPDAAIDDGVLDVVMLHPRRFLSWIPLAYRVLARRPRTDELVNRMTGHTVVVRASTDTPRQL
DGDSIGPGRELRMTCVHGRLLVRVPR

Sequences:

>Translated_506_residues
MFVLVTIAVQTGWGPVTSIDNRGQGAETWAVDERWLRLPLRWIEVGFGAIGTWVIVGVLAVLLFVRQHRRAAVYAVGVVA
VTKIATTILKAVFGRHRPDWQNPEHLLDSNAYPSGHSSTIVALVGVAAVLVMMLVRRRSARHTAYALLAVLAVVVGLDRV
LLGRHFPSDVVGGALLGAIVVLLGIVVQNPLPRSHAASVEPLPEVFGSEHRLTVILNPVKVEDVGQFEALVTAMAAEAGW
SAPTWHYTTVEDPGTGMAEAAAVAGTDLVLVCGGDGTVREVCAELAGTGIPVGIIPAGTGNLLARNLDIPLYLRAAIDVA
LTGQDRAIDLVEVSGDGFEDTHFMVMAGMGFDAAIMEGVNEDIKKRVGWLAYVLSALKSLMFPAVRVEVSVDGGEFTRHR
ARTIVVGNVGFLQAGMPLLPDAAIDDGVLDVVMLHPRRFLSWIPLAYRVLARRPRTDELVNRMTGHTVVVRASTDTPRQL
DGDSIGPGRELRMTCVHGRLLVRVPR
>Mature_506_residues
MFVLVTIAVQTGWGPVTSIDNRGQGAETWAVDERWLRLPLRWIEVGFGAIGTWVIVGVLAVLLFVRQHRRAAVYAVGVVA
VTKIATTILKAVFGRHRPDWQNPEHLLDSNAYPSGHSSTIVALVGVAAVLVMMLVRRRSARHTAYALLAVLAVVVGLDRV
LLGRHFPSDVVGGALLGAIVVLLGIVVQNPLPRSHAASVEPLPEVFGSEHRLTVILNPVKVEDVGQFEALVTAMAAEAGW
SAPTWHYTTVEDPGTGMAEAAAVAGTDLVLVCGGDGTVREVCAELAGTGIPVGIIPAGTGNLLARNLDIPLYLRAAIDVA
LTGQDRAIDLVEVSGDGFEDTHFMVMAGMGFDAAIMEGVNEDIKKRVGWLAYVLSALKSLMFPAVRVEVSVDGGEFTRHR
ARTIVVGNVGFLQAGMPLLPDAAIDDGVLDVVMLHPRRFLSWIPLAYRVLARRPRTDELVNRMTGHTVVVRASTDTPRQL
DGDSIGPGRELRMTCVHGRLLVRVPR

Specific function: Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is a key enzyme involved in the production of lipoteichoic acid by reintroducing DAG formed from the breakdown of membrane phospholipids into the phosphatidylglycerol biosynthet

COG id: COG1597

COG function: function code IR; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DAGKc domain [H]

Homologues:

Organism=Escherichia coli, GI1788402, Length=254, Percent_Identity=28.3464566929134, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017438
- InterPro:   IPR005218
- InterPro:   IPR001206 [H]

Pfam domain/function: PF00781 DAGK_cat [H]

EC number: =2.7.1.107 [H]

Molecular weight: Translated: 54333; Mature: 54333

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFVLVTIAVQTGWGPVTSIDNRGQGAETWAVDERWLRLPLRWIEVGFGAIGTWVIVGVLA
CEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
VLLFVRQHRRAAVYAVGVVAVTKIATTILKAVFGRHRPDWQNPEHLLDSNAYPSGHSSTI
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCHHH
VALVGVAAVLVMMLVRRRSARHTAYALLAVLAVVVGLDRVLLGRHFPSDVVGGALLGAIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
VLLGIVVQNPLPRSHAASVEPLPEVFGSEHRLTVILNPVKVEDVGQFEALVTAMAAEAGW
HHHHHHHCCCCCCCCCCCCCCHHHHCCCCCEEEEEECCEECCCCHHHHHHHHHHHHHCCC
SAPTWHYTTVEDPGTGMAEAAAVAGTDLVLVCGGDGTVREVCAELAGTGIPVGIIPAGTG
CCCCEEEEECCCCCCCHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEECCCC
NLLARNLDIPLYLRAAIDVALTGQDRAIDLVEVSGDGFEDTHFMVMAGMGFDAAIMEGVN
CCEEECCCCCEEEEEEEEEEECCCCCEEEEEEECCCCCCCCEEEEEECCCCHHHHHHCCC
EDIKKRVGWLAYVLSALKSLMFPAVRVEVSVDGGEFTRHRARTIVVGNVGFLQAGMPLLP
HHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHCCCCEEEEECCHHHHCCCCCCC
DAAIDDGVLDVVMLHPRRFLSWIPLAYRVLARRPRTDELVNRMTGHTVVVRASTDTPRQL
CCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEEEEECCCCCCCC
DGDSIGPGRELRMTCVHGRLLVRVPR
CCCCCCCCCCEEEEEECCEEEEEECC
>Mature Secondary Structure
MFVLVTIAVQTGWGPVTSIDNRGQGAETWAVDERWLRLPLRWIEVGFGAIGTWVIVGVLA
CEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
VLLFVRQHRRAAVYAVGVVAVTKIATTILKAVFGRHRPDWQNPEHLLDSNAYPSGHSSTI
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCHHH
VALVGVAAVLVMMLVRRRSARHTAYALLAVLAVVVGLDRVLLGRHFPSDVVGGALLGAIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
VLLGIVVQNPLPRSHAASVEPLPEVFGSEHRLTVILNPVKVEDVGQFEALVTAMAAEAGW
HHHHHHHCCCCCCCCCCCCCCHHHHCCCCCEEEEEECCEECCCCHHHHHHHHHHHHHCCC
SAPTWHYTTVEDPGTGMAEAAAVAGTDLVLVCGGDGTVREVCAELAGTGIPVGIIPAGTG
CCCCEEEEECCCCCCCHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEECCCC
NLLARNLDIPLYLRAAIDVALTGQDRAIDLVEVSGDGFEDTHFMVMAGMGFDAAIMEGVN
CCEEECCCCCEEEEEEEEEEECCCCCEEEEEEECCCCCCCCEEEEEECCCCHHHHHHCCC
EDIKKRVGWLAYVLSALKSLMFPAVRVEVSVDGGEFTRHRARTIVVGNVGFLQAGMPLLP
HHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHCCCCEEEEECCHHHHCCCCCCC
DAAIDDGVLDVVMLHPRRFLSWIPLAYRVLARRPRTDELVNRMTGHTVVVRASTDTPRQL
CCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEEEEECCCCCCCC
DGDSIGPGRELRMTCVHGRLLVRVPR
CCCCCCCCCCEEEEEECCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]